BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_008463	5,791,973	Δ1 bp	intergenic (+67/‑68)	nadE → / → choE	ammonia‑dependent NAD(+) synthetase/cholinesterase ChoE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_008463	5,791,973	0	A	.	100.0%	2731.3 / NA	549	intergenic (+67/‑68)	nadE/choE	ammonia‑dependent NAD(+) synthetase/cholinesterase ChoE
Reads supporting (aligned to +/- strand): ref base A (0/0); new base . (292/257); total (292/257)

Only 100 of 549 total aligned reads displayed.

GCGCGAGGAAGCCGCGCGGGTGATCGTCGACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCAACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTCAGCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAAAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCTGCTCCTCGGTGTCCTGCTGGCCGGCCAGGCCTGCGGACACACGTCCCCGCTGCT  >  NC_008463/5791828‑5792117

 cgcgAGGAAGCCGCGCGGGTGATCGTCGACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAAcc                                                                                                                                            <  1:1990460/150‑1 (MQ=255)

   cgAGGAAGCCGCGCGGGTGATCGTCGACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAg                                                                                                                                          >  2:2751939/1‑150 (MQ=255)

   cgAGGAAGCCGCGCGGGTGATCGTCGACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAg                                                                                                                                          >  2:2463433/1‑150 (MQ=255)

    gAGGAAGCCGCGCGGGTGATCGTCGACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGCCGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAgg                                                                                                                                         >  1:894491/1‑150 (MQ=255)

     aGGAAGCCGCGCGGGTGATCGTCGACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGt                                                                                                                                        <  2:6932442/150‑1 (MQ=255)

         aGCCGCGCGGGTGATCGTCGACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTc                                                                                                                                    >  2:223152/1‑150 (MQ=255)

           ccGCGCGGGTGATCGTCGACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCaa                                                                                                                                  <  1:2604645/150‑1 (MQ=255)

            cgcgcgGGTGATCGTCGACACCTACCCCAAGACCCAGCCCAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAg                                                                                                                                 <  2:7607823/150‑1 (MQ=255)

              cgcgGGTGATCGTCGACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTc                                                                                                                               <  2:2615436/150‑1 (MQ=255)

               gcgGGTGATCGTCGACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGACTGTGCCTGTC‑GCAACCAGGTCTTCAAGTcc                                                                                                                              >  2:10228557/1‑150 (MQ=255)

                  ggTGATCGTCGACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCcgcg                                                                                                                           >  1:1271864/1‑150 (MQ=255)

                   gTGATCGTCGACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCcgcgc                                                                                                                          <  2:9360104/150‑1 (MQ=255)

                    tGATCGTCGACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCcgcgcg                                                                                                                         <  1:10336197/150‑1 (MQ=255)

                    tGATCGTCGACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCcgcgcg                                                                                                                         >  1:8640039/1‑150 (MQ=255)

                     gATCGTCGACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCgcgg                                                                                                                        <  1:8021653/150‑1 (MQ=255)

                      aTCGTCGACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCgcggc                                                                                                                       >  1:3373594/1‑150 (MQ=255)

                        cgtcgACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCgcggcgg                                                                                                                     <  1:276755/150‑1 (MQ=255)

                        cgtcgACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCgcggcgg                                                                                                                     <  1:5656617/150‑1 (MQ=255)

                         gtcgACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCgcggcggc                                                                                                                    <  2:87786/150‑1 (MQ=255)

                          tcgACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCgcggcggcg                                                                                                                   >  1:5771727/1‑150 (MQ=255)

                           cgACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGgcgc                                                                                                                  >  2:1670271/1‑150 (MQ=255)

                            gACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGgcgcg                                                                                                                 >  2:4270142/1‑150 (MQ=255)

                             acacCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGgcgcgc                                                                                                                <  1:9484334/150‑1 (MQ=255)

                             acacCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGgcgcgc                                                                                                                <  2:1194682/150‑1 (MQ=255)

                               acCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTa                                                                                                              <  2:3189074/150‑1 (MQ=255)

                               acCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTa                                                                                                              >  2:5160370/1‑150 (MQ=255)

                               acCTACCACAAGACCCAGCACAAGCGCGAACAGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTa                                                                                                              <  1:5476698/150‑1 (MQ=255)

                                  tACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAGTGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTagaa                                                                                                           >  1:514520/1‑150 (MQ=255)

                                  tACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTagaa                                                                                                           >  2:5777132/1‑150 (MQ=255)

                                      acaAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGaa                                                                                                       <  2:1809182/150‑1 (MQ=255)

                                         aGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCGTCAAGTCCGCGCGGCGGCGCGCGAGACGGAAGCt                                                                                                    >  2:4017651/1‑150 (MQ=255)

                                         aGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCGTTGTCGCGGGCGGGTATGTGCCTGTC‑GCAACCAGGTCTTCATGTCCGCGCGGCGGCGCGCGAGAAggtcgct                                                                                                    >  1:181532/1‑145 (MQ=255)

                                          gACCCAGCTCATGCGCGACCTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAAGGTGCCTGTC‑GCAACCCGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTg                                                                                                   <  2:6309354/150‑1 (MQ=255)

                                           aCCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGACTGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGa                                                                                                  >  2:4462096/1‑150 (MQ=255)

                                            cccAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGaa                                                                                                 <  1:7583284/150‑1 (MQ=255)

                                                gCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATccc                                                                                             <  1:8813507/150‑1 (MQ=255)

                                                  acaAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTg                                                                                           <  1:6126812/150‑1 (MQ=255)

                                                  acaAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTg                                                                                           <  1:5556594/150‑1 (MQ=255)

                                                  acaAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTg                                                                                           <  1:134840/150‑1 (MQ=255)

                                                    aaGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCg                                                                                         >  1:4082418/1‑150 (MQ=255)

                                                        gcgAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAgg                                                                                     <  2:5657826/150‑1 (MQ=255)

                                                        gcgAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAgg                                                                                     >  2:3051756/1‑150 (MQ=255)

                                                         cgAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGACGCTGAATCCCTGCGAAGgc                                                                                    >  2:5240739/1‑150 (MQ=255)

                                                           aaCTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCTGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCGAGAAGGAAGCTGAATCCCTGCGAAGgcca                                                                                  >  1:4991700/1‑150 (MQ=255)

                                                           aaCTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCTTCGTCGACGGGCCTTTGTCGCGGGCGGGTATGTGCCTGTC‑GCAACCCGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGgcca                                                                                  <  2:7331228/150‑1 (MQ=255)

                                                           aaCTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGgcca                                                                                  >  2:1998838/1‑150 (MQ=255)

                                                            aCTGCCGAAGGCGCCCAGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGgccag                                                                                 <  1:1651708/150‑1 (MQ=255)

                                                               gCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGgccagcca                                                                              >  1:6837564/1‑150 (MQ=255)

                                                                ccGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCAt                                                                             >  2:6144783/1‑150 (MQ=255)

                                                                 cGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATg                                                                            >  1:9794235/1‑150 (MQ=255)

                                                                 cGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTGTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATg                                                                            >  2:6593162/1‑150 (MQ=255)

                                                                   aaGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATgcg                                                                          <  1:4487850/150‑1 (MQ=255)

                                                                    aGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATgcgc                                                                         <  2:8282130/150‑1 (MQ=255)

                                                                    aGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATgcgc                                                                         <  2:3297431/150‑1 (MQ=255)

                                                                        gcCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCAccc                                                                     <  2:8623052/150‑1 (MQ=255)

                                                                        gcCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCAccc                                                                     <  1:10000877/150‑1 (MQ=255)

                                                                         cccTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCg                                                                    <  1:10131298/150‑1 (MQ=255)

                                                                         cccTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCg                                                                    >  1:1188107/1‑150 (MQ=255)

                                                                           cTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGtc                                                                  >  1:2329746/1‑150 (MQ=255)

                                                                             gAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGtctc                                                                <  1:2277938/150‑1 (MQ=255)

                                                                             gAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGtctc                                                                <  1:148520/150‑1 (MQ=255)

                                                                                    ccGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGccc                                                         <  2:4105505/150‑1 (MQ=255)

                                                                                     cGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCt                                                        >  1:7094411/1‑150 (MQ=255)

                                                                                       gCCCGTGCGGCAGGCCCGGCGTCGACGGGCCTTTGTCGCGGGCGGGAAGGTGCCTGTC‑GCAACCCGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCTGc                                                      >  2:3638189/1‑150 (MQ=255)

                                                                                         ccGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCTGctc                                                    >  1:5257385/1‑150 (MQ=255)

                                                                                             gCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGATTCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCTGCTCCTCg                                                <  2:5823065/150‑1 (MQ=255)

                                                                                               ggCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGACCAGCCATGCGCACCCGTCTCCCCGCCCTGCTCCTCGgt                                              <  2:4973525/150‑1 (MQ=255)

                                                                                                  aGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCGAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCTGCTCCTCGGTGTc                                           >  1:6001396/1‑150 (MQ=255)

                                                                                                    gCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCTGCTCCTCGGTGTCct                                         >  1:7187873/1‑150 (MQ=255)

                                                                                                    gCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCTGCTCCTCGGTGTCct                                         >  2:3902991/1‑150 (MQ=255)

                                                                                                     cccGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCTGCTCCTCGGTGTCctg                                        >  1:6617490/1‑150 (MQ=255)

                                                                                                        gtcgtcGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCTGCTCCTCGGTGTCctgctg                                     >  2:8501880/1‑150 (MQ=255)

                                                                                                         tcgtcgACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCTGCTCCTCGGTGTCCTGCTgg                                    >  1:6374075/1‑150 (MQ=255)

                                                                                                         tcgtcgACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCTGCTCCTCGGTGTCCTGCTgg                                    >  1:927007/1‑150 (MQ=255)

                                                                                                          cgtcGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCCGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCTGCTCCTCGGTGTCCTGCTggc                                   <  2:1620014/150‑1 (MQ=255)

                                                                                                           gtcGACGGGCCTTTGTCGCGGGCGGGACTGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCTGCTCCTCGGTGTCCTGCTggcc                                  >  2:3453578/1‑150 (MQ=255)

                                                                                                           gtcGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCTGCTCCTCGGTGTCCTGCTggcc                                  >  1:3250372/1‑150 (MQ=255)

                                                                                                            tcgaCGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCTGCTCCTCGGTGTCCTGCTggccg                                 >  2:4204961/4‑150 (MQ=255)

                                                                                                            tcgaCGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCTGCTCCTCGGTGTCCTGCTggccg                                 >  1:3024545/4‑150 (MQ=255)

                                                                                                              gaCGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCTGCTCCTCGGTGTCCTGCTggccggc                               >  1:4746411/2‑150 (MQ=255)

                                                                                                              gaCGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCTGCTCCTCGGTGTCCTGCTggccggc                               <  2:10649175/149‑1 (MQ=255)

                                                                                                                cGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCTGCTCCTCGGTGTCCTGCTGGCCGGCCa                             <  2:8025459/150‑1 (MQ=255)

                                                                                                                cGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCTGCTCCTCGGTGTCCTGCTGGCCGGCCa                             >  1:8365393/1‑150 (MQ=255)

                                                                                                                cGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCTGCTCCTCGGTGTCCTGCTGGCCGGCCa                             >  2:6427964/1‑150 (MQ=255)

                                                                                                                 gggCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCTGCTCCTCGGTGTCCTGCTGGCCGGCCAg                            >  2:9614413/1‑150 (MQ=255)

                                                                                                                  ggCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCTGCTCCTCGGTGTCCTGCTGGCCGGCCAgg                           >  2:4626146/1‑150 (MQ=255)

                                                                                                                  ggCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCTGCTCCTCGGTGTCCTGCTGGCCGGCCAgg                           <  1:8181754/150‑1 (MQ=255)

                                                                                                                        tGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCTGCTCCTCGGTGTCCTGCTGGCCGGCCAGGCCTGCg                     <  1:10496821/150‑1 (MQ=255)

                                                                                                                        tGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCTGCTCCTCGGTGTCCTGCTGGCCGGCCAGGCCTGCg                     >  2:9421355/1‑150 (MQ=255)

                                                                                                                           cgcgGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCTGCTCCTCGGTGTCCTGCTGGCCGGCCAGGCCTGCGGac                  >  1:2460350/1‑150 (MQ=255)

                                                                                                                            gcgggcggGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCTGCTCCTCGGTGTCCTGCTGGCCGGCCAGGCCTGCGGaca                 >  1:4281136/1‑150 (MQ=255)

                                                                                                                                    gAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCTGCTCCTCGGTGTCCTGCTGGCCGGCCAGGCCTGCGGACACACGTccc         >  1:4018980/1‑150 (MQ=255)

                                                                                                                                    gAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCTGCTCCTCGGTGTCCTGCTGGCCGGCCAGGCCTGCGGACACACGTccc         >  1:7718253/1‑150 (MQ=255)

                                                                                                                                    gAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCTGCTCCTCGGTGTCCTGCTGGCCGGCCAGGCCTGCGGACACACGTccc         >  2:8875044/1‑150 (MQ=255)

                                                                                                                                    gAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCTGCTCCTCGGTGTCCTGCTGGCCGGCCAGGCCTGCGGACACACGTccc         >  2:9083593/1‑150 (MQ=255)

                                                                                                                                      aTGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCTGCTCCTCGGTGTCCTGCTGGCCGGCCAGGCCTGCGGACACACGTCCCCg       >  2:6737264/1‑150 (MQ=255)

                                                                                                                                       tgtgCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCAGGCGCACCCGTCTCCCCGCCCTGCTCCTCGGTGTCCTGCTGGCCGGCCAGGCCTGCGGACACACGTCCCCgc      <  1:2091380/150‑1 (MQ=255)

                                                                                                                                       ggtgCCTGTC‑GCAACCCGGTCTTCAAGTCCGCGCGGCGGCGCGCTCGACGGAAGCTGACTCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCTGCTCCTCGGTGTCCTGCGGGCCGGCCAGGCCTGCGGACACACGTCCCCgc      <  1:10633313/149‑1 (MQ=255)

                                                                                                                                           ccTGTC‑GCACCCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCTGCTCCTCGGTGTCCTGCTGGCCGGCCAGGCCTGCGGACACACGTCCCCgctgct  <  2:713921/150‑1 (MQ=255)

                                                                                                                                           ccTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCTGCTCCTCGGTGTCCTGCTGGCCGGCCAGGCCTGCGGACACACGTCCCCgctgct  >  2:7750909/1‑150 (MQ=255)

GCGCGAGGAAGCCGCGCGGGTGATCGTCGACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCAACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTCAGCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAAAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCTGCTCCTCGGTGTCCTGCTGGCCGGCCAGGCCTGCGGACACACGTCCCCGCTGCT  >  NC_008463/5791828‑5792117

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`