Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_008463114,8650TC31.8% 1357.0 / inf 718Y529H (TAC→CAC) vgrG1btype VI secretion system spike protein VgrG1b
Reads supporting (aligned to +/- strand):  ref base T (245/244);  new base C (115/113);  total (360/358)
Fisher's exact test for biased strand distribution p-value = 9.36e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.19e-02
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 724 total aligned reads displayed.

GCGGCACGCCGGCCAACTTCAACGAGATCCGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAAGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATAACCATCGGCGTCGACCGCACCGAGAAGGTCGGTAACAACGAGAAGATCAGTATCGGCGCGAACCGCACCGAGGACGTCGGCAGCAAC  >  NC_008463/114716‑115014
                                                                                                                                                     |                                                                                                                                                     
  ggcACGCCGGCCAACTTCAACGAGATCCGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAAGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTac                                                                                                                                                     >  1:9535476/1‑150 (MQ=255)
    cACGCCGGCCAACTTCAACGAGATCCGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAAGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAATACCATCGACTacga                                                                                                                                                   >  2:237121/1‑150 (MQ=255)
     aCGCCGGCCAACTTCAACGAGATCCGCATGGAGGACACGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAAGACCCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTacgac                                                                                                                                                  <  2:8916834/150‑1 (MQ=255)
     aCGCCGGCCAACTTCAACGAGATCCGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAGGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCacgac                                                                                                                                                  <  1:8220307/150‑1 (MQ=255)
        ccGGCCAACTTCAACGAGATCCGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAGGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACgag                                                                                                                                               <  1:6536761/150‑1 (MQ=255)
           gCCAACTTCAACGAGATCCGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAGGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACg                                                                                                                                            >  2:6587695/1‑150 (MQ=255)
           gCCAACTTCAACGAGATCCGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAGGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACg                                                                                                                                            >  2:2782672/1‑150 (MQ=255)
            ccAACTTCAACGAGATCCGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAGGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACgg                                                                                                                                           >  2:7919237/1‑150 (MQ=255)
             cAACTTCAACGAGATCCGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAGGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGt                                                                                                                                          >  2:3956439/1‑150 (MQ=255)
             cAACTTCAACGAGATCCGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAAGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGt                                                                                                                                          <  2:9592128/150‑1 (MQ=255)
                cTTCAACGAGATCCGCATGGAGGACACGAAGGGCGCCGAGCAGTGGTTCATCCATGCCGAGAAGAACCAGGACATCGAGTTCGAGAACGCCGAGAGCCACTGGGTCGGCCACTACCGGACCAAGACCATCGACTACGACGAGACGGTGCa                                                                                                                                       <  2:2624886/150‑1 (MQ=14)
                  tCAACGAGATCCGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAGGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCAtg                                                                                                                                     >  2:5094638/1‑150 (MQ=255)
                  tCAACGAGATCCGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAGGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCAtg                                                                                                                                     >  1:3663985/1‑150 (MQ=255)
                  tCAACGAGATCCGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAAGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCAtg                                                                                                                                     <  2:7235959/150‑1 (MQ=255)
                    aaCGAGATCCGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAAGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCAtgtg                                                                                                                                   <  2:1523608/150‑1 (MQ=255)
                     aCGAGATCCGCTTGGAGGACAAGAAGGGCTCCGAGCATTTGTTCATCCATGCCGAGAGGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGa                                                                                                                                  <  2:8137419/150‑1 (MQ=11)
                       gagaTCCGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAAGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAg                                                                                                                                <  1:1213711/150‑1 (MQ=255)
                        agaTCCGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAGGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGc                                                                                                                               <  1:10376478/150‑1 (MQ=255)
                        agaTCCGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAGGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGc                                                                                                                               >  1:1806760/1‑150 (MQ=255)
                         gaTCCGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAAGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCa                                                                                                                              <  2:1740616/150‑1 (MQ=255)
                          aTCCGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAGGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCAc                                                                                                                             <  2:7562822/150‑1 (MQ=255)
                             cGCATGGAGGACAAGAAGGGCGCCTAGCAGTTGTTCATCCAGGCCGAGAAGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGAt                                                                                                                          <  2:8322132/150‑1 (MQ=255)
                             cGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAGGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGAt                                                                                                                          <  1:3543559/150‑1 (MQ=255)
                             cGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAAGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGAt                                                                                                                          <  2:3146400/150‑1 (MQ=255)
                              gCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAAGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATc                                                                                                                         >  2:7497314/1‑150 (MQ=255)
                               cATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAGGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCg                                                                                                                        >  1:6717529/1‑150 (MQ=255)
                               cATGGACGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAGGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCg                                                                                                                        <  2:1982865/150‑1 (MQ=255)
                                aTGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAGGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGc                                                                                                                       <  2:9074166/150‑1 (MQ=255)
                                aTGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAGGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGc                                                                                                                       <  1:3184060/150‑1 (MQ=255)
                                aTGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAGGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGc                                                                                                                       >  1:7465550/1‑150 (MQ=255)
                                 tGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAGGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCa                                                                                                                      <  2:1144976/150‑1 (MQ=255)
                                 tGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAGGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCa                                                                                                                      >  1:7362200/1‑150 (MQ=255)
                                  ggaggaCAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAAGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCAc                                                                                                                     >  1:2720656/1‑150 (MQ=255)
                                      gaCAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAAGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCgag                                                                                                                 >  1:1000042/1‑150 (MQ=255)
                                          agaagGGCGCCGAGCAGTTGTTCATCCATGCCGAGAGGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCg                                                                                                             >  1:1425672/1‑150 (MQ=255)
                                          agaagGGCGCCGAGCAGTTGTTCATCCATGCCGAGAGGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCg                                                                                                             <  2:2477943/150‑1 (MQ=255)
                                           gaagGGCGCCGAGCAGTTGTTCATCCATGCCGAGAGGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGt                                                                                                            >  1:4217576/1‑150 (MQ=255)
                                            aagGGCGCCGAGCAGTTGTTCATCCATGCCGAGAAGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTg                                                                                                           >  1:2436989/1‑150 (MQ=255)
                                            aagGGCGCCGAGCAGTTGTTCATCCATGCCGAGAAGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTg                                                                                                           >  2:8811098/1‑150 (MQ=255)
                                             agGGCGCCGAGCAGTTGTTCATCCATGCCGAGAGGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTgg                                                                                                          >  1:4571357/1‑150 (MQ=255)
                                              gggCGCCGAGCAGTTGTTCATCCATGCCGAGAGGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGa                                                                                                         >  2:6334426/1‑150 (MQ=255)
                                               ggCGCCGAGCAGTTGTTCATCCATGCCGAGAAGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGac                                                                                                        >  1:2176656/1‑150 (MQ=255)
                                                 cgcCGAGCAGTTGTTCATCCATGCCGAGAAGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGacaa                                                                                                      <  1:8366782/150‑1 (MQ=255)
                                                   ccGAGCAGTTGTTCATCCATGCCGAGAGGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGacaaca                                                                                                    >  1:921829/1‑150 (MQ=255)
                                                   ccGAGCAGTTGTTCATCCATGCCGAGAAGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGacaaca                                                                                                    >  1:4107486/1‑150 (MQ=255)
                                                    cGAGCAGTTGTTCATCCATGCCGAGAGGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGAcaacaa                                                                                                   >  1:2960553/1‑150 (MQ=255)
                                                    cGAGCAGTTGTTCATCCATGCCGAGAAGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGAcaacaa                                                                                                   >  1:2560691/1‑150 (MQ=255)
                                                      aGCAGTTGTTCATCCATGCCGAGAAGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACg                                                                                                 >  1:4956869/1‑150 (MQ=255)
                                                       gCAGTTGTTCATCCATGCCGAGAAGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACga                                                                                                >  1:9339267/1‑150 (MQ=255)
                                                       gCAGGTGTACATCCAAGCCGAGAGGAACCAGGACATCGAGGTCGAGAACGCCGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCAACGCCCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACga                                                                                                <  2:9374475/150‑1 (MQ=11)
                                                        cAGTTGTTCATCCATGCCGAGAAGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACgag                                                                                               >  1:5145196/1‑150 (MQ=255)
                                                         aGTTGTTCATCCATGCCGAGAGGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACgaga                                                                                              >  2:7037848/1‑150 (MQ=255)
                                                          gttgttCATCCATGCCGAGAGGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGAc                                                                                             >  1:5831176/1‑150 (MQ=255)
                                                           ttgttCATCCATGCCGAGAGGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTTGACAACAACGAGAcc                                                                                            <  2:2080671/150‑1 (MQ=255)
                                                           ttgttCATCCAAGCCGAGAAGAACCAGGACATCGAGGTCGACAACGACGAGAGCCACTGCGTCGGCCACGACCGGACCAAGACCAACGACTACGACGAGACGGTGCATGTCAAGCACGAACGCACCGAGACCGAGGACAACAACGAGAcc                                                                                            >  2:7715809/1‑150 (MQ=14)
                                                              ttCATCCATGCCGAGAAGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATa                                                                                         >  2:10649206/1‑150 (MQ=255)
                                                                cATCCATGCCGAGAGGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCAc                                                                                       <  1:2051438/150‑1 (MQ=255)
                                                                 aTCCATGCCGAGAAGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATAAcc                                                                                      <  2:1388900/150‑1 (MQ=255)
                                                                  tCCATGCCGAGAAGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATAACCa                                                                                     >  2:4820438/1‑150 (MQ=255)
                                                                  tCCATGCCGAGAAGAACCAGGACAACGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGAGGACAACAACGAGACCAGAACCa                                                                                     >  2:5347410/1‑150 (MQ=255)
                                                                    cATGCCGAGAAGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATAACCATc                                                                                   <  2:103138/150‑1 (MQ=255)
                                                                    cATGCCGAGAAGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATAACCATc                                                                                   <  2:4359650/150‑1 (MQ=255)
                                                                     aTGCCGAGAGGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCAccgtgc                                                                                  >  2:4166754/1‑146 (MQ=255)
                                                                     aTGCCGAGAGGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCAccgtgc                                                                                  >  1:5422745/1‑146 (MQ=255)
                                                                      tGCCGAGAGGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCAccgtgca                                                                                 >  2:294263/1‑145 (MQ=255)
                                                                       gCCGAGAAGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATAACCATCGGc                                                                                >  2:3627706/1‑150 (MQ=255)
                                                                        ccGAGAGGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCAccgtgcatg                                                                               <  1:9904367/150‑8 (MQ=11)
                                                                         cGAGAAGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATAACCATCGGCGt                                                                              >  1:6294714/1‑150 (MQ=255)
                                                                         cGAGAAGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATAACCATCGGCGt                                                                              <  1:2825357/150‑1 (MQ=255)
                                                                         cGAGAAGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATAACCATCGGCGt                                                                              <  2:6799098/150‑1 (MQ=255)
                                                                          gagaAGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATAACCATCGGCGTc                                                                             >  1:9716396/1‑150 (MQ=255)
                                                                          gagaAGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATAACCATCGGCGTc                                                                             >  1:6176066/1‑150 (MQ=255)
                                                                          gagaAGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATAACCATCGGCGTc                                                                             >  1:4368260/1‑150 (MQ=255)
                                                                           agaagaACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATAACCATCGGCGTCg                                                                            >  1:6213305/1‑150 (MQ=255)
                                                                            gaagaaCCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATAACCATCGGCGTCGa                                                                           <  1:6095232/150‑1 (MQ=255)
                                                                                  ccAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATAACCATCGGCGTCGACCGCAc                                                                     <  2:454057/150‑1 (MQ=255)
                                                                                  ccAGGACATCGACGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATAACCATCGGCGTCGACCGCAc                                                                     <  2:334064/150‑1 (MQ=255)
                                                                                    aGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATAACCATCGGCGTCGACCGCACCg                                                                   >  1:7671587/1‑150 (MQ=255)
                                                                                         aTCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATAACCAACGGCGTCGACCGCACCGAGAAg                                                              >  2:3442910/1‑150 (MQ=255)
                                                                                            gAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATAACCATCGGCGTCGACCGCACCGAGAAGGTc                                                           >  1:5626677/1‑150 (MQ=255)
                                                                                             aGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATAACCATCGGCGTCGACCGCACCGAGAAGGTCg                                                          >  1:3782179/1‑150 (MQ=255)
                                                                                                tCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATAACCATCGGCGTCGACCGCACCGAGAAGGTCGGTa                                                       <  1:8602777/150‑1 (MQ=255)
                                                                                                      acgacgAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATAACCATCGGCGTCGACCGCACCGAGAAGGTCGGTAACAACg                                                 <  2:2287458/150‑1 (MQ=255)
                                                                                                      acgacgAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATAACCATCGGCGTCGACCGCACCGAGAAGGTCGGTAACAACg                                                 >  1:8954994/1‑150 (MQ=255)
                                                                                                         acgaGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATAACCATCGGCGTCGACCGCACCGAGAAGGTCGGTAACAACgaga                                              >  1:3403856/1‑150 (MQ=255)
                                                                                                          cgaGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATAACCATCGGCGTCGACCGCACCGAGACGGTCGGGAACAACGagaa                                             >  2:10046282/1‑150 (MQ=255)
                                                                                                                 cACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATAACCATCGGCGTCGACCGCACCGAGAAGGTCGGTAACAACGAGAAGATCAGt                                      <  2:1609093/150‑1 (MQ=255)
                                                                                                                   cTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATAACCATCGGCGTCGACCGCACCGAGAAGGTCGGTAACAACGAGAAGATCAGTAt                                    <  1:10202393/150‑1 (MQ=255)
                                                                                                                     gggTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATAACCATCGGCGTCGACCGCACCGAGAAGGTCGGTAACAACGAGAAGATCAGTATCg                                  >  1:7932314/1‑150 (MQ=255)
                                                                                                                      ggTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATAACCATCGGCGTCGACCGCACCGAGAAGGTCGGTAACAACGAGAAGATCAGTATCgg                                 <  2:6166651/150‑1 (MQ=255)
                                                                                                                      ggTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATAACCATCGGCGTCGACCGCACCGAGAAGGTCGGTAACAACGAGAAGATCAGTATCgg                                 <  1:10711688/150‑1 (MQ=255)
                                                                                                                          ggCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATAACCATCGGCGTCGACCGCACCGAGAAGGTCGGTAACAACGAGAAGATCAGTATCGGcgcg                             >  2:5713711/1‑150 (MQ=255)
                                                                                                                                    ggACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATAACCATCGGCGTCGACCGCACCGAGAAGGTCGGTAACAACGAGAAGATCAGTATCGGCGCGACCCGCACCg                   >  1:7009569/1‑150 (MQ=255)
                                                                                                                                       ccAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATAACCATCGGCGTCGACCGCACCGAGAAGGTCGGTAACAACGAGACGATCAGAATCGGCGCGAACCGCACCGAgg                >  2:5986806/1‑150 (MQ=255)
                                                                                                                                       ccAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATAACCATCGGCGTCGACCGCACCGAGAAGGTCGGTAACAACGAGACGATCAGAATCGGCGCGAACCGCACCGAgg                >  2:9744005/1‑150 (MQ=255)
                                                                                                                                        cAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATAACCATCGGCGTCGACCGCACCGAGAAGGTCGGTAACAACGAGAAGATCAGTATCGGCGCGAACCGCACCGAGGa               >  2:10146618/1‑150 (MQ=255)
                                                                                                                                           gACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATAACCATCGGCGTCGACCGCACCGAGAAGGTCGGTAACAACGAGAAGATCAGTATCGGCGCGAACCGCACCGAGGACGt            >  1:169557/1‑150 (MQ=255)
                                                                                                                                            aCCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATAACCATCGGCGTCGACCGCACCGAGAAGGTCGGTAACAACGAGAAGATCAGTATCGGCGCGAACCGCACCGAGGACGTc           <  2:3851278/150‑1 (MQ=255)
                                                                                                                                                  gACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATAACCATCGGCGTCGACCGCACCGAGAAGGTCGGTAACAACGAGAAGATCAGTATCGGCGCGAACCGCACCGAGGACGTCGgcagc     >  1:10480386/1‑150 (MQ=255)
                                                                                                                                                     tACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATAACCATCGGCGTCGACCGCACCGAGAAGGTCGGTAACAACGAGAAGATCAGTATCGGCGCGAACCGCACCGAGGACGTCGGCAGCAAc  <  1:6823383/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GCGGCACGCCGGCCAACTTCAACGAGATCCGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAAGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATAACCATCGGCGTCGACCGCACCGAGAAGGTCGGTAACAACGAGAAGATCAGTATCGGCGCGAACCGCACCGAGGACGTCGGCAGCAAC  >  NC_008463/114716‑115014

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑