Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084634,616,4540TG33.3% 282.4 / 19.9 154intergenic (‑174/+28)hda/PA14_RS21180DnaA regulatory inactivator Hda/DUF2066 domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base T (56/44);  new base G (50/0);  total (110/44)
Fisher's exact test for biased strand distribution p-value = 3.72e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 159 total aligned reads displayed.

AACGCCGCAGGAGTCACCGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGCG‑CCGGCCCGCTCC‑GTTGCATAGGAGCGGATCGGCGCGCCGACTCACTGCCAGCGGAAATTCA‑GTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTACGCTGTCGGCCGGGATTTCCTGCAACTGCGCCAGGCCCA  >  NC_008463/4616305‑4616603
                                                                                                                                                       |                                                                                                                                                      
aaCGCCGCAGGAGTCACCGTAAAGGCTGCAAAGTTTATACGCCTCGGCACGGCCGCACACTGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGCG‑CCGGCCCGCTCC‑Gt                                                                                                                                                        <  2:3510885/150‑1 (MQ=255)
 aCGCCGCAGGAGTCACCGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAACATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGCG‑CCGGCCCGCTCC‑Gtt                                                                                                                                                       <  2:5898499/150‑1 (MQ=255)
 aCGCCGCAGGAGTCACCGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGCG‑CCGGCCCGCTCC‑Gtt                                                                                                                                                       <  1:5006900/150‑1 (MQ=255)
    ccgcAGGAGTCACCGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGGG‑CCGGCCCCCACC‑Gtttga                                                                                                                                                    >  2:883771/1‑147 (MQ=255)
      gcAGGAGTCACCGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGCG‑CCGGCCCGCTCC‑GTTGCATa                                                                                                                                                  <  1:2398583/150‑1 (MQ=255)
       cAGGAGTCACCGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTATGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGATCCTTGCCCAGAGCAACGGCCGGTCGCGCG‑CCGGCCCGCTCA‑GTTTCaaat                                                                                                                                                 >  2:7751511/1‑147 (MQ=255)
        aGGAGTCACCGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCATAGCATCGGCCGGTCGCGCG‑CCCGCCCCCACC‑CGTGCATAgg                                                                                                                                                >  2:6144084/1‑150 (MQ=255)
        aGGAGTCACCGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGCG‑GCGGCCCCCGCC‑GGGGGATAgg                                                                                                                                                >  1:5072781/1‑150 (MQ=255)
          gAGTCACCGTCAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGGG‑CCGGGCCGCTCC‑GGTGGATAGGAg                                                                                                                                              >  2:6797441/1‑150 (MQ=255)
             tCACCGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGCG‑CCGGCCCGCTCC‑GTTGCATAGGAGCgg                                                                                                                                           <  1:2934178/150‑1 (MQ=255)
               aCCGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGCG‑CCGGCCCGCTCC‑GTTGCATAGGAGCGGAt                                                                                                                                         <  1:9285140/150‑1 (MQ=255)
                ccGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAACGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGCG‑CCGGCCCGCTCC‑GTTGCATAGGAGCGGATc                                                                                                                                        <  1:3695894/150‑1 (MQ=255)
                ccGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGCG‑CCGGCCCGCTCC‑GTTGCATAGGAGCGGATc                                                                                                                                        <  1:3574443/150‑1 (MQ=255)
                 cGTAAAGGCTGCACAGTTTATCCGCCTCCTCCAGGCCGCACACTGAGCAACTAGGCAAGCATAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCTGTCGCGCG‑CCGGCCCGCACC‑GTTGCATAGGAGCGGATCg                                                                                                                                       <  2:2314573/150‑1 (MQ=255)
                 cGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGGG‑CCGGGCCGCTCC‑CGTTCAAATGAGCggctag                                                                                                                                       >  2:7170909/1‑146 (MQ=255)
                 cGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGCG‑CCGGCCCGCTCC‑GTTGCATAGGAGCGGATCg                                                                                                                                       <  2:3455956/150‑1 (MQ=255)
                 cGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGCG‑CCGGCCCGCTCC‑GTTGCATAGGAGCGGATCg                                                                                                                                       <  1:5654627/150‑1 (MQ=255)
                 cGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACGAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGCG‑CCGGCCCGCTCC‑GTTGCATAGGAGCGGATCg                                                                                                                                       <  1:5672163/150‑1 (MQ=255)
                 cGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAAAGAGCAACTAGGCAAGCAAAATCAAAGGTTGTCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGCG‑ACGGCCCGCTCC‑GTTGCATAGGAGCGGATCg                                                                                                                                       >  2:5122520/1‑150 (MQ=255)
                  gTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGCG‑CCGGCCCGCCCC‑GGTGCCAAGGTGCGGaaagg                                                                                                                                      >  2:4338708/1‑146 (MQ=255)
                   tAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGCG‑CCGGCCCGCTCC‑GTTGCATAGGAGCGGATCGgc                                                                                                                                     <  1:7509395/150‑1 (MQ=255)
                     aaGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGCGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGCG‑CCGGCCCGCTCC‑GTTGCATAGGAGCGGATCGgcgc                                                                                                                                   <  2:9400924/150‑1 (MQ=255)
                      aGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGCG‑CCGGGCCGCGCC‑GTTGCATATGAGCGGAACCgcgcc                                                                                                                                  >  2:8750911/1‑149 (MQ=255)
                      aGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGCG‑CCGGCCCGCGCC‑GGTGCATAGGAGCGGATCGgcgcg                                                                                                                                  >  2:8749786/1‑150 (MQ=255)
                        gCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGCG‑GCGGCCCCCGGC‑GGTGGATATGTGCGGATGGGGGCGcc                                                                                                                                >  1:9414704/1‑150 (MQ=255)
                         cTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGGG‑CCGGCCCGCGGC‑CTGGGAAAGGAGCGGAGAGGCGCGCCg                                                                                                                               >  2:938819/1‑150 (MQ=255)
                         cTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGCG‑CCGGCCCGCTCC‑GTTGCATAGGAGCGGATCGGCGCGCCg                                                                                                                               <  2:6789063/150‑1 (MQ=255)
                          tGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGGG‑CCGGCCCCCTCC‑CGTGGGAAGGAGCGGAGAGGCGCGcccc                                                                                                                              >  2:5369481/1‑148 (MQ=255)
                          ggCAACGTTAATACGCCTCGGAAAGCCCGCACAATGTGAAACTAGGCAAGCAACATCAACGTTTTGCCGATAGCCGAGGCTCCTTGCCCAGTGCATCGGCCGTTCGCGCG‑CCGGCCCGCACG‑GTTGAATAGGTGCGTATCGGCGCGCCGa                                                                                                                              <  2:9194594/149‑1 (MQ=255)
                           gCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGGG‑CCGGCCCGCACC‑CTTGCTAATGAGGGGGTCGGGGCGCCCAc                                                                                                                             >  2:7248336/1‑150 (MQ=255)
                             aaaGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGCG‑CCCGCCCGCTAC‑GATGGATATGAGCGGATCGGCGCGCCGAcgc                                                                                                                           >  2:4737925/1‑148 (MQ=255)
                             aaaGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGCG‑CCCGCCCGCGCC‑CGTGCCTAGGAGCGGATCGGCGCGCCGAcac                                                                                                                           >  2:10148828/1‑148 (MQ=255)
                                     taCGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGCG‑GCGGCCCGCTCC‑GGTGCATATGAGCGGATAGGGGCGGCGCCTCAAAGCCAg                                                                                                                   >  1:10543771/1‑150 (MQ=255)
                                     taCGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGCG‑CCGGCCCCCGCC‑GGTGGCTAGGTGCGGTTGGGCGCGCCGGCTCCCTGCCAg                                                                                                                   >  1:10545115/1‑150 (MQ=255)
                                        gCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGGG‑CCCGCCCGCGCC‑GGTGGAGGGGTGGGGAGGGGCGCGCCCGCGCACAGCCCGCgg                                                                                                                >  1:2624969/1‑150 (MQ=255)
                                         ccTCGGCAAGGCCGCACAATGAGCAACTAGGCACGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGCG‑CCGGCCCCCGCC‑GGTGCCGAGGAGGGGCTCGGCGCGCCGACACACAGCCCGCCGa                                                                                                               >  2:10533431/1‑150 (MQ=255)
                                          cTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGGG‑CCGGCCCCCGCC‑GGTGCCGAGGGGCGGGTCGGCGCGCCCCCTGACAGCCCGCggga                                                                                                              >  2:7607587/1‑148 (MQ=255)
                                           tCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGGG‑CCGGCCCGCGCC‑GGTGGATAGTATCGGATCGGGGCGCCGACACAAAGACAGCGGaaa                                                                                                             >  1:1768685/1‑150 (MQ=255)
                                              gCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGGG‑CCCGGCCGCGGC‑CGTGGATAGGTGGGGATCGGCGCCCCGCCACACAGCCAGCGGgaaatg                                                                                                          >  1:6115335/1‑147 (MQ=255)
                                                      gCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGCG‑CCGGCCCCCGCC‑CTTGGCTAGGAGGGGGTAGGGGCGCCGCCACACAGCCCGCGGGAAATAA‑GTTgggg                                                                                                 >  2:2432659/1‑149 (MQ=255)
                                                        acaATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGCGCCCGGCCCCGCCC‑GTGGCA‑AGGAGCGGGTCGGCGCGCCCCCACACAGACCGCGGGAATTGA‑GGTGGggcg                                                                                               >  2:2936185/1‑150 (MQ=255)
                                                         caATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGCG‑CCCGCCCCCTCC‑GGTGGCTAGGGGCGGATCGGGGCGCCGACACACAGCCAACGGAAATTCA‑GTTGGgacga                                                                                              >  2:9553470/1‑150 (MQ=255)
                                                           aTGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGCG‑CCGGCCCGCACC‑CTTGCATAGGGGCGGATAGGGGCGCCGACACAAAGCCCGCGGGAATTAA‑GGTGGgtcgagg                                                                                            >  2:7971743/1‑148 (MQ=255)
                                                            tGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGGG‑CCGGCCCGCTCC‑GATGGAGAGGTGCGGGTAGGCGCGCCGAAGCACATCCAGCGGAAATTAA‑TTTGGggagacga                                                                                           >  1:8619296/1‑150 (MQ=255)
                                                             gAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGCG‑CCGGCCCCCACC‑CGTTCAAAGGAGCGGATAGGCGCGCCCACACACAGCCACCCCAAAATAA‑GTTGGggccacgca                                                                                          >  1:8773788/1‑148 (MQ=255)
                                                               gCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGCG‑CCGGCCCGCGCC‑GGTGGATTGGAGCGGGTAGGCGCCCCCCCTCACAGCCCCCGGGAATTGA‑GGTTGgacgaacaccg                                                                                        >  2:3082317/1‑144 (MQ=255)
                                                                 aaCTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGGG‑CCCGGCCGGTCC‑CGTGGCTAGGTGCGGCTGGGCGCCCCGGCACACAGCCCGCGGAAATAAA‑GTTGGggcgacgcccgcc                                                                                      >  2:5584114/1‑144 (MQ=255)
                                                                  aCTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGGG‑GCGGGCCGCGCC‑GTTGGAAATTTTGGGATCGGCGCGCCCGCGGACAGGCACCGGGAAATAA‑GGTGGGTCGACGACCGCAc                                                                                     >  2:8150837/1‑150 (MQ=255)
                                                                  aCTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGCG‑CCGGCCCGCGCC‑GGTGCATAGGGGCGGGTCGGCGCGCCGACACACAGCCCGCGGTAATTAA‑GGTGGGGCGACGACGGCAc                                                                                     >  2:808809/1‑150 (MQ=255)
                                                                  aCTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGCG‑CCGGCCCCCAAC‑CTTTCATATGAGCGGATACGCGCGCCGACACACAGCCACCCGAAAATAA‑GTTTTGACGACCACGGCAc                                                                                     >  2:9568009/1‑150 (MQ=255)
                                                                           gcaaAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGCG‑CCGGCCCCCCCC‑CGTGGAAAGGGGCGGGTAGGGGCGCCGCCGCAAAGACACCGGAGAATAA‑GTTTGGGCGACCACCGCAcccccccccg                                                                            >  2:4590967/1‑145 (MQ=255)
                                                                            caaAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGGG‑GCGGCCCGCGCC‑GGTGGAAAGGAGCGGGTCGGCGCGCCGCCGCACAGCCCGCGGGAAATAA‑CTTGGGGCGGCGACGGCACCCCCccgcgg                                                                           >  1:7093866/1‑148 (MQ=255)
                                                                            caaAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGCC‑GCGGCCCGCACC‑GTTGCCTACGCGCCGAGAGGCGCGCCCCCCCACAGCCACCCGCAAATCA‑GTTGGGCCGTCGACGGCACCCCCgcgcag                                                                           >  2:7589126/1‑148 (MQ=255)
                                                                                 tCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGCC‑CCCGCCCCCGCC‑CGTTCATATGATCGGATAGGCCCGCCCCCACACATCCACCGGAAAATAA‑GTTTGGACGACGACCGCACCCCCCCGCAGGgcgc                                                                      >  2:4416955/1‑150 (MQ=255)
                                                                                   aaaGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGCG‑CCGGCCCCCTCC‑CTTTCAAAGGAGCGGCTCGGGGCGCCCACACACAGCCCGCGGAAATTGA‑GTTTGGGCGAAGAAGGCACCCCCCCGCCTGgcgcgg                                                                    >  2:7128934/1‑148 (MQ=255)
                                                                                    aaGGTTTGCCGATAGCCGATGCTCCTTGCCCAGAGCATCGGCCGGTCGCGCG‑CCGGCCCCCGCCGGTTAAATTGGTG‑GGGTGGGCGCGCCGCAACACATCCCGCCGCAAGTAA‑GGTTGGGCGACGCGAGCACCGCCGCGCGGGGCGCGGc                                                                   >  2:2016075/1‑150 (MQ=255)
                                                                                        tttGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGCG‑GCCGCCCGCGCC‑GGTGGCTAGGAGCGGATAGGCGCGCCCCCACACAGCCCCCGGGCATTAA‑GGGGGGGCGACGACCGCACCGCCGCGCAGGGGGCGGCGGGc                                                               >  2:563066/1‑150 (MQ=255)
                                                                                        tttGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGGCGCGGG‑CCGGGCCGCGGC‑GTTGGCGTGGGGCGGGTAGGGGCGCCGACGCACTGCCCGCCGTACATCA‑GGTGGGACGACGGCCGCCCCGCCGCGCAGGGGGCGGCgggg                                                               >  2:3344353/1‑149 (MQ=255)
                                                                                          tGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGCG‑CCCGCCCCCGCC‑CGAGCAGTCGAGCCGCGAGGCGCGCCGCCACCAAGCCCGCGGAAAGAAA‑GTTGGGGAGACAACCGCACCCCCGCGCGGGGCGCGGCGGGGTg                                                             >  2:4429792/1‑150 (MQ=255)
                                                                                           gcgtatcgCCGCGGCTCCTTGCCCATTGCATCGGCCGGCCGCGCG‑CCGCCCCGCTCG‑GTTGCATTGGCGGGGAGGGGCGCCCCGACTCACTGCCAGCGGAAATTCA‑GTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTgg                                                            <  2:5727995/143‑1 (MQ=255)
                                                                                            cctaTAGCCGTGGCACCTGCCCCACCGAACCGGCGGGTCGCGCG‑CCGCCCCGCTCG‑GTTGCATCGCCGCGCTCCGGCGCCCCGACTCACTGCCAGCGGAAATTCA‑GTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGc                                                           <  2:9653422/147‑1 (MQ=255)
                                                                                              gATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGGG‑GCGGCCCGCGCC‑GGTGGCTTGGAGCGGGGCGGCGCGCCCGCACACAGCCCGCGGGAAATGA‑GGTGGGGCGACGACGGCCCCCCCGCGCGGGGGGGTGCggggggggcg                                                         >  2:9360460/1‑143 (MQ=255)
                                                                                              gATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGGG‑CCGGCCCGCGCC‑CGGGGCTAGGAGCGGCTCGGGGCGGCGGAGCGCAGCCCCCGGACATTAA‑GTTGGGGCCTCGACCGCACCCCCCCGCGGGGGGCGGCGGGGGGGCCg                                                         >  2:497929/1‑150 (MQ=255)
                                                                                               aTAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGGG‑CCGGGCCCCACC‑CGTGCAAATGAGCGGTGCGGCGCGCCGACACACCGCCCCCGGAAAATGA‑GGTGGGGCGGCCACGGCAACCCCCCGCGGGGGGCGGCGGGGTGGGCgg                                                        >  2:5080799/1‑150 (MQ=255)
                                                                                                  gCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGGG‑CCCGCCCCCGCC‑CGTGCCTAGGAGAGGCTAGGCGGGCCCACACACAAACAGCCGAAAATGA‑GTTTGGCCGAAGACGGCACCGCCCCCCGGGGGGCGGGGGGGGGGCCCGAgg                                                     >  2:4000588/1‑150 (MQ=255)
                                                                                                    cGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGGG‑CCGGCCCCCCCC‑GTTTCATATGCGCGGATAGGGGCCGCGCCACACTTCCACCCGAAATTTACTTTGGGAC‑TCGACCGCACCCCCCCCCAGGGCGCGGGGGGCGGGGCGGGGGcc                                                   >  2:8832972/1‑149 (MQ=255)
                                                                                                           ccgggCCCAGAGCAGCGGCCGCCCGCGCG‑CGGCCCCCCTCC‑GTTGCAGCGCCGCGGCCCGGCGCCCCGACTCACTGCCAGCGGAAATACC‑GTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCg                                            <  2:10512946/146‑1 (MQ=255)
                                                                                                             ttGCCCAGAGCATCGGCCGGTCGCGGG‑GCCGGCCGCACC‑CTTTCATAAGAGCGGATAGGCGCGCCGACACAAAGCCCGCCGAAAATAA‑CTTTGGTCCAACCAGGCACCCCCCCCCCTGGGGGTGGCGGCTGGCCGGGGGCATCCAGccgg                                          >  2:3958973/1‑147 (MQ=255)
                                                                                                                   tgtgactCGGCGGGCCGCGCG‑CGGCCCCGCCCG‑TTTGATTTGGCGCGGAAGGGCGCCCCGACTCACTGCCAGCGGAAATTCA‑GTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTACGCTg                                    <  1:791222/144‑1 (MQ=255)
                                                                                                                   agcgctccGGCCGCGCGCGCG‑CCGGCCCGCTCG‑GTTGCATCGGCGCGCAGCGCCCCCCCGACTCACTGCCAGCGGAAATTCA‑GTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTACGCTg                                    <  1:6994844/143‑1 (MQ=255)
                                                                                                                     ccccgcggcggGCCGCCCG‑CCGGCCCGACCG‑TTTCCTTCGGCGCGGATCGGCCCCCCGACTCACTGCCAGCGGAAATTCA‑GTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTACGCTGTc                                  <  1:5237701/145‑1 (MQ=255)
                                                                                                                     agCTTCGGCGGGTGGCGCG‑CCGCCCCGCTCG‑TTTTAATTGGGGGGTCTCGGCCCCCCGACTCACTGCCAGCGGAAATTCA‑GTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTACGCTGTc                                  <  1:5242710/150‑1 (MQ=255)
                                                                                                                                 tCGCGCG‑CCGGCCCGCTCC‑GTTGCATAGGAGCGGATCGGCGCGCCGACTCACTGCCAGCGGAAAATCA‑GTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCCTCCAGCGGTACGCTGTCGGCCGGGATTTc                      >  2:10164364/1‑150 (MQ=255)
                                                                                                                                 tCGCGCG‑CCGGCCCGCTCC‑GTTGCAAAGGAGCGGATCGGCGCGCCGACTCACTGCCCGCGGACAATCA‑GTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCAGGCCGGAGGCATCCAGCGGTACGCAGTCGGCCGGGATTTc                      >  2:10164464/1‑150 (MQ=255)
                                                                                                                                   gcgcg‑cCGGCCCGCTCC‑GTTGCATAGGAGCGGATCGGCGCGCCGACTCACTGCCAGCGGAAATTCA‑GTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTACGCTGTCGGCCGGGATTTCCt                    >  1:2527980/1‑150 (MQ=255)
                                                                                                                                    cgcg‑cCGGCCCGCTCC‑GTTCAATCGCTGCTGCTCGGCGCCCCGACTCACTGCCAGCGGAAATTCA‑GTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTACGCTGTCGGCCGGGATTTCCTg                   <  2:4081203/150‑1 (MQ=255)
                                                                                                                                     gcg‑cCGGCCCGCTCC‑GTTGCATAGGAGCGGATCGGCGCGCCGACTCACTGCCAGCGGAAATTCC‑GTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTACGCTGGCGGCCGGGATTTCCTGc                  >  1:10256998/1‑150 (MQ=255)
                                                                                                                                     gcg‑cCGGCCCGCTCC‑GTTGCATAGGAGCGGATCGGCGCGCCGACTCACTGCCAGCGGAAATTCA‑GTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTACGCTGTCGGCCGGGATTTCCTGc                  >  2:3672352/1‑150 (MQ=255)
                                                                                                                                     gcg‑cCGGCCCGCTCC‑GTTGCATAGGAGCGGATCGGCGCGCCGACTCACTGCCAGCGGAAATTCA‑GTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTACGCTGTCGGCCGGGATTTCCTGc                  >  1:3546549/1‑150 (MQ=255)
                                                                                                                                     gcg‑cCGGCCCGCTCC‑GTTGCATAGGAGCGGATCGGCGCGCCGACACACTGCCAGCGGAAATTCA‑GTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTACGCTGTCGGCCGGGATTTCCTGc                  >  1:3547821/1‑150 (MQ=255)
                                                                                                                                      cg‑cCGGCCCGCTCC‑GTTGCATAGGAGCGGATCGGCGCGCCGACTCACTGCCAGCGGAAATTCA‑GTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTACGCTGTCGGCCGGGATTTCCTGCa                 >  2:8367674/1‑150 (MQ=255)
                                                                                                                                       g‑cCGGCCCGATCG‑GTTGAATCGTCGCGGTCCGCCCCCCCGACTCACTGCCAGCGGAAATTCA‑GTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTACGCTGTCGGCCGGGATTTCCTGCaa                <  2:9266934/150‑1 (MQ=255)
                                                                                                                                         ccGGCCCGCTCC‑GTTGCATAGGAGCGGATCGGCGCGCCGACTCACTGCCAGCGGAAATTCA‑GTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTACGCTGTCGGCCGGGATTTCCTGCAAc               >  1:1403638/1‑150 (MQ=255)
                                                                                                                                         ccGGCCCGCTCC‑GTTGCATAGGAGCGGAACGGCGCGCCGACTCACTGCCAGCGGAAATTCA‑GTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTACGCTGTCGGCCGGGATTTCCTGCAAc               >  1:3045814/1‑150 (MQ=255)
                                                                                                                                         ccGGCCCGCCCT‑GTTCCTTTGGCGCGGATCGGCGCCCCGACTCACTGCCAGCGGAAATTCA‑GTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTACGCTGTCGGCCGGGATTTCCTGCAAc               <  2:1652144/150‑1 (MQ=255)
                                                                                                                                          cgccccgcccg‑GTTCCAGAGGAGCGGTGCGCCGCCCCGACTCACTGCCAGCGGAAATTCA‑GTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTACGCTGTCGGCCGGGATTTCCTGCAACt              <  2:8995804/147‑1 (MQ=255)
                                                                                                                                            gCCCGCTCC‑GTTGCATAGGAGCGGATCGGCGCGCCGACTCACTGCCAGCGGAAATTCA‑GTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTACGCTGTCGGCCGGGATTTCCTGCAACTgc            >  1:9306125/1‑150 (MQ=255)
                                                                                                                                            gCCCGCTCC‑GTTGCATAGGAGCGGATCGGCGCGCCGACTCACTGCCAGCGGAAATTCA‑GTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTACGCTGTCGGCCGGGATTTCCTGCAACTgc            >  1:6600474/1‑150 (MQ=255)
                                                                                                                                             cccGCTCC‑GTTGCATAGGAGCGGATCGGCGCGCCGACTCACTGCCAGCGGAAATTCA‑GTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTACGCTGTCGGCCGGGATTTCCTGCAACTgcg           >  1:8127014/1‑150 (MQ=255)
                                                                                                                                             cccGCTCC‑GTTGCATAGGAGCGGATCGGCGCGCCGACTCACTGCCAGCGGAAATTCA‑GTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTACGCTGTCGGCCGGGATTTCCTGCAACTgcg           >  1:401077/1‑150 (MQ=255)
                                                                                                                                              ccGCTCC‑GTTGCATAGGAGCGGATCGGCGCGCCGACTCACTGCCAGCGGAAATTCA‑GTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTACGCTGTCGGCCGGGATTTCCTGCAACTgcgc          >  2:5293241/1‑150 (MQ=255)
                                                                                                                                              ccGCTCC‑GTTGCATAGGAGCGGATCGGCGCGCCGACTCACTGCCAGCGGAAATTCA‑GTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTACGCTGTCGGCCGGGATTTCCTGCAACTgcgc          >  2:3460009/1‑150 (MQ=255)
                                                                                                                                               cGCTCC‑GTTGCCTAGGAGCGGATCGGCGCGCCGACTCACTGCCAGCGGAAATTCA‑GTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTACGCTGTCGGCCGGGATTTCCTGCAACTGCGcc         >  2:2071798/1‑150 (MQ=255)
                                                                                                                                                 cTCC‑GTTGCATAGGAGCGGATCGGCGCGCCGACTCACTGCCAGCGGACATTCA‑GTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTACGCTGTCGGCCGGGATTTCCTGCAACTGCGCCAg       >  1:9954403/1‑150 (MQ=255)
                                                                                                                                                 cTCC‑GTTGCATAGGAGCGGATCGGCGCGCCGACTCACTGCCAGCGGAAATTCA‑GTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTACGCTGTCGGCCGGGATTTCCTGCAACTGCGCCAg       >  1:6470152/1‑150 (MQ=255)
                                                                                                                                                  tCC‑GTTGCATAGGAGCGGATCGGCGCGCCGACTCACTGCCAGCGGAAATTCA‑GTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTACGCTGTCGGCCGGGATTTCCTGCAACTGCGCCAgg      >  1:2166947/1‑150 (MQ=255)
                                                                                                                                                  tCC‑GTTGCATAGGAGCGGATCGGCGCGCCGACTCACTGCCAGCGGAAATTCA‑GTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTACGCTGTCGGCCGGGATTTCCTGCAACTGCGCCAgg      >  1:8573725/1‑150 (MQ=255)
                                                                                                                                                   cc‑GTTGCATAGGAGCGGATCGGCGCGCCGACTCACTGCCAGCGGAAATTCA‑GTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTACGCTGTCGGCCGGGATTTCCTGCAACTGCGCCAGGc     >  2:8534890/1‑150 (MQ=255)
                                                                                                                                                   cc‑GTTGCATAGGAGCGGATCGGCGCGCCGACTCACTGCCAGCGGAAATTCA‑GTTGGGACGACGACCGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTACGCTGTCGGCCGGGATTTCCTGCAACTGCGCCAGGc     >  1:10434172/1‑150 (MQ=255)
                                                                                                                                                    cggTTGCATAGGTGCGGAGCGGCGCCCCGACTCACTGCCAGCGGAAATTCA‑GTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTACGCTGTCGGCCGGGATTTCCTGCAACTGCGCCAGGc     <  2:8234747/148‑1 (MQ=255)
                                                                                                                                                       |                                                                                                                                                      
AACGCCGCAGGAGTCACCGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGCG‑CCGGCCCGCTCC‑GTTGCATAGGAGCGGATCGGCGCGCCGACTCACTGCCAGCGGAAATTCA‑GTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTACGCTGTCGGCCGGGATTTCCTGCAACTGCGCCAGGCCCA  >  NC_008463/4616305‑4616603

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑