Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084635,910,2560AC27.4% 582.9 / 44.0 280intergenic (+55/‑90)aceE/aceFpyruvate dehydrogenase (acetyl‑transferring), homodimeric type/dihydrolipoyllysine‑residue acetyltransferase
Reads supporting (aligned to +/- strand):  ref base A (137/64);  new base C (1/75);  total (138/142)
Fisher's exact test for biased strand distribution p-value = 2.40e-27
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 291 total aligned reads displayed.

TGGCCGATCGCGGCGATATCGAGCCGAAAGTGGTGGCCGAGGCCATCGCCAAGTACGGTATCGATCCGGAGAAGCGCAACCCGCTGGATTGCTAATTCGCTCCATACTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGCCGCCAAC‑GGG‑CGGCGCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAGCGGTGGTGGCACGTAAAAGGAGAGGAATGTGAGCGAACTCATTCGCGTACCCGACATCGGCAACGGTGAGGGTGAAGTCATCGAGCTG  >  NC_008463/5910107‑5910405
                                                                                                                                                     |                                                                                                                                                       
tGGCCGATCGCGGCGATATCGAGCCGAAAGTGGTGGCCGAGGCCATCGCCAAGTACGGTATCGATCCGGAGAAGCGCAACCCGCTGGATTGCTAATTCGCTCCATACTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGCCGCCaa                                                                                                                                                         <  1:9784535/150‑1 (MQ=255)
 ggCCGATCGCGGCGATATCGAGCCGAAAGTGGTGGCCGAGGCCATCGCCAAGTACGGTATCGATCCGGAGAAGCGCAACCCGCTGGATTGCTAATTCGCTCCATACTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGCCGCCAAc                                                                                                                                                        <  2:8501730/150‑1 (MQ=255)
  gCCGATCGCGGCGATATCGAGCCGAAAGTGGTGGCCGAGGCCATCGCCAAGTACGGTATCGCTCCGGAGAAGCGCAACCCGCTGGATTGCTAATTCGCTCCATACTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGCCGCCAAC‑g                                                                                                                                                      <  1:6578421/150‑1 (MQ=255)
  gCCGATCGCGGCGATATCGAGCCGAAAGTGGTGGCCGAGGCCATCGCCAAGTACGGTATCGATCCGGAGAAGCGCAACCCGCTGGATTGCTAATTCGCTCCATACTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGCCGCCAAC‑g                                                                                                                                                      <  1:9307417/150‑1 (MQ=255)
  gCCGATCGCGGCGATATCGAGCCGAAAGTGGTGGCCGAGGCCATCGCCAAGTACGGTATCGATCCGGAGAAGCGCAACCCGCTGGATTGCTAATTCGCTCCATACTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGCCGCCAAC‑g                                                                                                                                                      <  2:9135670/150‑1 (MQ=255)
    cGATCGCGGCGATATCGAGCCGAAAGTGGTGGCCGAGGCCATCGCCAAGTACGGTATCGATCCGGAGAAGCGCAACCCGCTGGATTGCTAATTCGCTCCATACTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGCCGCCAAC‑ggg                                                                                                                                                    <  1:167648/150‑1 (MQ=255)
    cGATCGCGGCGATATCGAGCCGAAAGTGGTGGCCGAGGCCATCGCCAAGTACGGTATCGATCCGGAGAAGCGCAACCCGCTGGATTGCTAATTCGCTCCATACTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGCCGCCAAC‑ggg                                                                                                                                                    <  1:4678239/150‑1 (MQ=255)
     gATCGCGGCGATATCGAGCCGAAAGTGGTGGCCGAGGCCATCGCCAAGTACGGTATCGATCCGGAGAAGCGCAACCCGCTGGATTGCTAATTCGCTCCATACTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGCCGCCAAC‑Ggg‑c                                                                                                                                                  <  2:6843422/150‑1 (MQ=255)
       tCGCGGCGATATCGAGCCGAAAGTGGTGGCCGAGGCCATCGCCAAGTACGGTATCGATCCGGAGAAGCGCAACCCGCTGGATTGCTAATTCGCTCCATACTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGCCGCCAAC‑Ggg‑cgg                                                                                                                                                >  2:2501410/1‑150 (MQ=255)
       tCGCGGCGATATCGAGCCGAAAGTGGTGGCCGAGGCCATCGCCAAGTACGGTATCGATCCGGAGAAGCGCAACCCGCTGGATTGCTAATTCGCTCCATACTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGCCGCCAAC‑Ggg‑cgg                                                                                                                                                >  2:836315/1‑150 (MQ=255)
          cggcgATATCGAGCCGAAAGTGGTGGCCGAGGCCATCGCCAAGTACGGTATCGATCCGGAGAAGCGCAACCCGCTGGATTGCTAATTCGCTCCATACTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGCCGCCAAC‑GGG‑CGgcgc                                                                                                                                             >  2:1496935/1‑150 (MQ=255)
                tatCGAGCCGAAAGTGGTGGCCGAGGCCATCGCCAAGTACGGTATCGATCCGGAGAAGCGCAACCCGCTGGATTGCTAATTCGCTCCATACTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGCCGCCAAC‑GGG‑CGGCGCCGGCTc                                                                                                                                       >  2:4189962/1‑150 (MQ=255)
                 atCGAGCCGAAAGTGGTGGCCGAGGCCATCGCCAAGTACGGTATCGATCCGGAGAAGCGCAACCCGCTGGATTGCTAATTCGCTCCATACTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGCCGCCCAC‑CGG‑CGGCGCCGGCGca                                                                                                                                      >  1:627200/1‑150 (MQ=255)
                 atCGAGCCGAAAGTGGTGGCCGAGGCCATCGCCAAGTACGGTATCGATCCGGAGAAGCGCAACCCGCTGGATTGCTAATTCGCTCCATACTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGCCGCCAAC‑CGG‑CCGCGCCGGCTca                                                                                                                                      >  1:2346447/1‑150 (MQ=255)
                     aGCCGAACGTGGTGGCGGTGGCCTTCGCCACGTACGGTATCGTTCCGGTTACGCCCAACCGGCTGTTTGGCTAATTCGCTCCAGCCGCCCCTGGGCAGCGAGGGGGTTCGACCACGCGGGCGCCGCCACC‑GGG‑CGGCGCCGGCTCACAAc                                                                                                                                  <  2:6934422/150‑1 (MQ=255)
                       ccGAAAGTGGTGGCCGAGGCCATCGCCAAGTACGGTATCGATCCGGAGAAGCGCAACCCGCTGGATTGCTAATTCGCTCCATACTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCCCCGCCCAC‑GGG‑CGGCGCCGGCGAACAACGa                                                                                                                                >  1:2180707/1‑150 (MQ=255)
                       ccGAAAGTGGTGGCCGAGGCCATCGCCAAGTACGGTATCGATCCGGAGAAGCGCAACCCGCTGGAGTGCTAATTCGCTCCATACTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGCCGCCAAC‑GGG‑CGGCGCCGGCTCACAACGa                                                                                                                                >  2:10393888/1‑150 (MQ=255)
                          aaaGTGGTGGCCGAGGCCATCGCCAAGTACGGTATCGATCCGGAGAAGCGCAACCCGCTGGATTGCTAATTCGCTCCATACTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGCCGCCAAC‑GGG‑CGGCGCCGGCTCACAACGAAcc                                                                                                                             >  2:2622908/1‑150 (MQ=255)
                          aaaGTGGTGGCCGAGGCCATCGCCAAGTACGGTATCGATCCGGAGAAGCGCAACCCGCTGGATTGCTAATTCGCTCCATACTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGCCGCCAAC‑CGG‑CGGCGCCGGCTcacacagaaac                                                                                                                             >  1:13986/1‑143 (MQ=255)
                              gggtgcCCGTGGCCATCTCAATTTCCGGTATCTATCCGGAGATGCTAACCCCGGTGTTTTGATAATTCGCTCAATACTCCCCTCGGCTGGGTGGGTTTTCTCCCACGCGGGCGCCGCAACC‑GGG‑GGGCGCCGGCTCACAACGAACCAAgg                                                                                                                         <  2:5017838/149‑1 (MQ=255)
                               ggtggCCGAGGCCATCGCCAAGTACGGTATCGATCCGGAGAAGCGCAACCCGCTGGATTGCTAATTCGCTCCATACTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGCCGCCAAC‑GGG‑CGGCGCCGGCGCACAACGAACCAAGGa                                                                                                                        >  2:388918/1‑150 (MQ=255)
                                    ccGAGGCCATCGCCAAGTACGGTATCGATCCGGAGAAGCGCAACCCGCTGGATTGCTAATTCGCTCCATACTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGCCGCCAAC‑GGG‑CGGCGCCGGCTCACAACGAACCAAGGACCaaa                                                                                                                   >  1:6625574/1‑150 (MQ=255)
                                       aGGCCATCGCCAAGTACGGTATCGATCCGGAGAAGCGCAACCCGCTGGATTGCTAATTCGCTCCATACTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGCCGCCAAC‑CGG‑CGGCGCCGGCTCACAACCAACCAATGACCaaactt                                                                                                                >  1:9164927/1‑147 (MQ=255)
                                          ccAACGCCTTGTACGGTAAGGATCCGGCCAAGCCCAACCCTCTGTTTTGCAAATTCGCTCATTACTCCCCGCGGCCGCTAGGGGTTTCGCGCACGCCGGCGCCCCCACC‑GGG‑CGGCGCCGGCTCACAACGAACCAAGGACCAAAAGTCtt                                                                                                             <  2:9451445/150‑1 (MQ=255)
                                              cGCCAAGTACGGTATCGATCCGGAGAAGCGCAACCCGCTGGATTGCTAATTCGCTCCATACTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGCCGCCAAC‑GGG‑CGGCGCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGtt                                                                                                         >  2:4374587/1‑150 (MQ=255)
                                                ccAAGTACGGTATCGATCCGGAGAAGCGCAACCCGCTGGATTGCTAATTCGCTCCATACTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGCCGCCAAC‑GGG‑CGGCGCCGGCTCACAACGAACCCAGGACCAAAAGTCTTTGtttt                                                                                                       >  2:6612872/1‑150 (MQ=255)
                                                  aaGAACGGTACGGATCGGGAGATGCCAAACCCGCTGTATTCATAATTCGCTCCATACGCCCCTGGGCAGCGTGGTGTTTCGACCCCGCGGGCGCCGCCAAC‑GGG‑CGGCGCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTgc                                                                                                     <  2:7111153/150‑1 (MQ=255)
                                                     tACGGTATCGATCCGGAGAAGCGCAACCCGCTGGATTGCTAATTCGCTCCATACTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGCCGCCAAC‑GGG‑CGGCGCCGGCTCACAACGAACCAAGGACCAAAAGTATTTGTTTTtcgcg                                                                                                  >  2:445501/1‑150 (MQ=255)
                                                       cGGTATCGACCCGGAGACGCGCAACCCGCTGGTTTGCTAATTCGCTCAATCCGCCCCCCGGCAGCGTGGGGTTTCGCGCACGCCGGCGCCGCCACC‑GGG‑CGGCCCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGcgcc                                                                                                <  2:4078688/150‑1 (MQ=255)
                                                        ggTATCGATCCGGAGAAGCGCAACCCGCTGGATTGCTAATTCGCTCCATACTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGCCGCCAAC‑GGG‑CGGCGCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGcgccg                                                                                               >  2:7969844/1‑150 (MQ=255)
                                                        ggTATCGATCCGGAGAAGCGCAACCCGCTGGATTGCTAATTCGCTCCATACTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGCCGCCAAA‑CGG‑CGGCCCCCGCTAACAACGAACCAAGGAACCAAAGTATTTTTTTTTCGcgccc                                                                                               >  1:3367892/1‑149 (MQ=255)
                                                           ttCGATCCGGAGACGCGCAACCCGGTGGTTTGCTAATGCGCTCCATCCTCCCCTCGGCTGGGTGGGGTTTCGCGCACGCCGGCGCCGCCAAC‑GGG‑CGGCGCCGGCTCACAACGAACCAAGGACCAACAGTCTTTGTTTTGCGCGCCGcac                                                                                            <  2:2346158/149‑1 (MQ=255)
                                                           aTCGATCCGGAGAAGCGCAACCCGCTGGATTGCTAATTCGCTCCATACTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGCCGCCAAC‑GGG‑GGGCGCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGcac                                                                                            >  1:8602217/1‑150 (MQ=255)
                                                            tCGATCCGGAGAAGCGCAACCCGCTGGATTGCTAATTCGCTCCATACTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGCCGCCCAC‑GGG‑CGGCGCCGGCTCACAACGAACCAAGGACCAAAAGTGTTTGGTTTGCGCGCCGcaca                                                                                           >  2:5257818/1‑150 (MQ=255)
                                                            gcGATCCTGAGACGCGCACCCCGCTGGATTGCTAATGCGCTCCATACTCCCCGCGCCAGCGCGGGGTTTCCAGCCCGCCGGCGCCGCCAAC‑GGG‑CGGCGCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGcaca                                                                                           <  2:9580771/149‑1 (MQ=255)
                                                              taTCGGGAGACGCGAAACCCGCTGGATTGCTAATTCGCTCAATCCGCCCCTCGCCAGCGTGGGTTTTCGCGCACGCCGGCGCCGCCAAC‑GGG‑CGGCGCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAGc                                                                                         <  2:10422637/149‑1 (MQ=255)
                                                               aTCCGGAGAAGCGCAACCCGCTGGATTGCTAATTCGCTCCATACTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGCCCCCCAC‑GGG‑CGGCGCCGGCGCACAACCAAACAAGGACCCAAAGTCTTTGTTTTTCGCGCCGCCCAGCg                                                                                        >  1:4280422/1‑150 (MQ=255)
                                                                gcggcagaCGCGCACCCCCCTGGTGTCCTAATTCCCGCCACACCCCCCGGGGCTGCGTGGGGTTTCGCGCACGCCGGCGCCGCCACC‑GGG‑CGGCGCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAGCgg                                                                                       <  2:8927669/145‑1 (MQ=255)
                                                                 ccGGAGAAGCGCAACCCGCTGGATTGCTAATTCGCTCCATACTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGCCGCCAAC‑GGG‑CGGCGCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAGCggt                                                                                      >  2:8392178/1‑150 (MQ=255)
                                                                  cGGAGACGCCCACCCCGCGGGATTGATAATCCGCCCCATACTCCCCTCGGCAGCGTGGGGTTACGCGCCCGCCGGCCCCGCCACG‑GGG‑CGGCCCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAGCggtg                                                                                     <  1:7464196/150‑1 (MQ=255)
                                                                  cGGAGAAGCGCAACCCGCTGGATTGCTAATTCGCTCCATACTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGCCGCCAAC‑GGG‑CGGCGCCGGCTCACAACGAAACAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAGAggtg                                                                                     >  2:284234/1‑150 (MQ=255)
                                                                   ggAGAAGCGCAACCCGCTGGATTGCTAATTCGCTCCATACTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGCCGCCAAC‑GGG‑CGGCGCCGGCTCACAACGAACCAAGGACCAAAAGTGTTTGTTTTGCGCGCCGCACAGCggtgg                                                                                    >  1:9712212/1‑150 (MQ=255)
                                                                      gacccgaaaCCCGCTGGATTGCTACTTCGCTCCACACCCCCCGCGGCAGGGTGGGGTAGCGCGCCCGCCGGCGCCGCCACG‑GGG‑CGGCGCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAGCGGtggtgg                                                                                 <  2:8515686/143‑1 (MQ=255)
                                                                         gcccaccccGCTGTATCGCTAATGCCCCCCCACCCCCCCCGGGCAGCGTGGGGTTTCCCGCCCGCCGGCGCCCCCCCG‑GGG‑CGGCGCCGGCTCACAACGAACCAAGGACCACAAGTCTTTGTTTTGCGCGCCGCACAGCGGTGGTGGCAc                                                                              <  1:6858244/144‑1 (MQ=255)
                                                                           ccAACCCGCTGGATTGCAAATGCGCACAACACTCCCCTCGGCAGCGTGGGGTTTCGAGCACGCCGCCGCCGCCACC‑GGG‑CGGCCCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAGCGGTGGTGGCACGt                                                                            <  2:9933451/149‑1 (MQ=255)
                                                                            cAACCCGCTGGATTGCTAATTCGCTCCATACTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGGCGCCCAC‑CGG‑CGGCGCCCGCGCACCACCAACCCAGGACCCAAAGTCTTTGTGTTGGGCGCCGCCCCGCGGgggtggggcggt                                                                           >  1:10259853/1‑144 (MQ=255)
                                                                            cAACCCGCTGGATTGCTAATTCGCTCCATACTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGCCGCCCAC‑CGG‑CGGGGCCGGCACAAAAAGAAACAAGGACCAAAATTGGTTGTTTTGCGCGCCGCACAGCGGgggtgggccggt                                                                           >  2:5605884/1‑144 (MQ=255)
                                                                               cccGCTGGATTGCTAATTCGCTCCATACTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGCCGCCAAC‑GGG‑CGGCGCCGGCTCACAACGAACCAAGGACCAAAAGTATTTGTTTTGCGCGCCGCACCGCGGTGGTGGGCCGGaaaa                                                                        >  1:8009336/1‑150 (MQ=255)
                                                                                ccGCTGTTTTGCTAATGCGCACAATCCCCCCCTCGGCCGCGCGGGGTTGCGAGCCCGCCGGCGCCGCCACC‑GGG‑GGGCGCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAGCGGTGGTGGCACGTAAAAg                                                                       <  2:2042566/150‑1 (MQ=255)
                                                                                ccGCTGGTTTGATATTACCCACAATCCCCCCCTCGGCTGGGTGGGTTTCCGAGCACGCCGGCCCCCCCACG‑GGG‑GGCCCCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAGCGGTGGTGGCACGTAAAAg                                                                       <  1:1342228/150‑1 (MQ=255)
                                                                                ccGCTGGATTGCTAATTCGCTCCATACTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGCCGCCAAC‑GGG‑CGGCGCCGGCTCACAACGCACCAAGGAACCAAAGTGTTTGTTTTGCGCGCCGCACAGCGGTGGTGGCACGTAAAAg                                                                       >  2:8981265/1‑150 (MQ=255)
                                                                                  gCTGGATTGCTAATTCGCTCCATACTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGCCGCCAAC‑GGG‑CGGCGCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAGCGGTGGTTGGACGGAAAATga                                                                     >  2:7218485/1‑150 (MQ=255)
                                                                                       tttGCTAATTCGCACCATACTCCCCTCGGCAGCGAGGGTTTTCGAGCACGCCGGCGCCGCCACC‑GGG‑CGGCGCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAGCGGTGGTGGCACGTAAAAGGAGAGGa                                                                <  2:6546525/149‑1 (MQ=255)
                                                                                       aTTGCTAATTCGCTCCATACTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGCCGCCAAC‑GGG‑CGGCGCCGGCTCACCACGAAACAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACCGCGGTGGTGGCACGTAAAAGGAGAGGa                                                                >  2:9333824/1‑150 (MQ=255)
                                                                                         tGCTAATTCGCTCCATACTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGCCGCCAAC‑GGG‑CGGCGCCGGCTCACAACGAACCAAGGACCAAAATTCTTTGTTTTGCGCGCCGCACAGCGGTGGTGGCACGGAAAAGGAGAGGAAt                                                              >  2:6069544/1‑150 (MQ=255)
                                                                                          gCTAATTCGCTCCATACTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGCCCCCCAC‑CGG‑GGGCGGCGGCACACAAACAAACCAGGTACAAAAGTAATTGTTTTGCGCGGCGCACAGCGGGGGTGGGACGTGATAGGGGTGGGAtg                                                             >  1:3002601/1‑150 (MQ=255)
                                                                                            tAATTCGCTCCATACTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGCCGCCAAC‑GGG‑CGGCGCCGGGTCACAACGAACCCAGGTCCAAAAGGGGTTGTTTTTCGCGCCGCACAGCGGGGGGGGGAGGTAGAAGGAGAGGAAtgtg                                                           >  1:5616618/1‑150 (MQ=255)
                                                                                             aaTTCGCTCCATACTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGCCGCCCAC‑CGG‑CGGCGCCGGCTCACAACGAACCAAGGGCCCAAATTCTTTTTTTTGCGCGGCCCACAGCGGTGGTGGCACGGAAAAGGAGAGGACtgtgg                                                          >  1:5848387/1‑149 (MQ=255)
                                                                                             aaTTCGCTCCATACTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGCCGCCACC‑CGG‑CGGCGCCGGCTCCCAACGAACCAAGGACCAAAAGTCTATGTTTTGCGCGCCGCACAGCGGGGGTGGCACGTCAAAGGAGAGGAAtgtgt                                                          >  2:5001884/1‑149 (MQ=255)
                                                                                              aTTCGCTCCATACTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGCCGCCCAC‑GGG‑CGGCGCCGGCTCACAACGAACCAAGGTCCAAAAGTCTTTGTTTTGCGCGCCGCACCGCGGTGGTGGCACGTAAAAGGAGAGGAATGTGAg                                                         >  1:8821711/1‑150 (MQ=255)
                                                                                              aTTCGCTCCATACCCCCCTGGGCTGCGTGGGGTTTCGCCCACGCCGGCGCCGCCAAC‑GGG‑CGGCGCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAGCGGTGGTGGCACGTAAAAGGAGAGGAATGTGAg                                                         <  2:7631184/150‑1 (MQ=255)
                                                                                               ttCGCTCCATACTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGCCGCCAAC‑GGG‑CGGCGCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAGCGGTGGTGGCACGTAAAAGGAGAGGAATGTGAGc                                                        >  2:10439809/1‑150 (MQ=255)
                                                                                               tgcGCCCCAGACTCCCCTCGGCCGCGAGGGGTTCCGCGCACGCCGGCGCCCCCACC‑GGG‑CGGCGCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAGCGGTGGTGGCACGTAAAAGGAGAGGAATGTGAGc                                                        <  2:4876960/148‑1 (MQ=255)
                                                                                                 cGCTCCATACTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGCCGCCAAC‑GGG‑CGGCGCCGGCGCACAACGAACCAAGGTCCAAAAGTCTTTGTTTTGCGCGCCCCACAGCGGTGGTGGCACGGAAAAGGGGAGGACAGTGTGCGa                                                      >  2:2812505/1‑150 (MQ=255)
                                                                                                   ccccACACACCCCGCGGCCGCGTGGGTTTCCGCCCCCGCCGGCGCCCCAATC‑GGG‑CGGCGCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAGCGGTGGTGGCACGTAAAAGGAGAGGAATGTGAGCGAAc                                                    <  2:3665910/148‑1 (MQ=255)
                                                                                                      catccgccccTGGGCTGCGTGGGTTTTCGAGCACCCCGGCGCCGACACC‑GGG‑CGGCGCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAGCGGTGGTGGCACGTAAAAGGAGAGGAATGTGAGCGAACTCa                                                 <  2:9667883/144‑1 (MQ=255)
                                                                                                      cATACTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGCCGCCAAC‑GGG‑CGGCGCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACCGCGGTGGTGGCCCGTAAAAGGAGAGGAATGTGAGCGTACtaa                                                 >  2:7689956/1‑148 (MQ=255)
                                                                                                         aCTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGCCGCCAAC‑GGG‑GGGGGCCGGCTCACAACGAACCAAAGACCAAAAGTCTTTGTTTTGCGCGGCGCACAGCGGTGGTGGCACGTGAAAGGAGAGGGCTGTGAGCGAACTCATTc                                              >  1:7890430/1‑150 (MQ=255)
                                                                                                            ccccTCGGCCGCGCGGGGTTTCGCCCCCGCCGGCGCCGCCACC‑GGG‑CGGCGCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAGCGGTGGTGGCACGTAAAAGGAGAGGAATGTGAGCGAACTCATTcgcg                                           <  2:1618018/150‑1 (MQ=255)
                                                                                                                   gCAGCGAGGGGTTTCGAGCACGCCGGCGCCGCCAAC‑GGG‑CGGCGCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAGCGGTGGTGGCACGTAAAAGGAGAGGTATGTGAGCGTACACAATAGCGTACCCGa                                    >  2:3229259/1‑150 (MQ=255)
                                                                                                                   ccAGCGTGGGGTTTCCCCCCCCCCGGCGCCCCCCCG‑GGG‑CGGCCCCGGCTCACACCGAACCAAGGCCCAAAAGTCTTTGTATTGCGCGCCGCACAGCGGTGGTGGCACGTAAAAGGAGAGGAATGTGAGCGAACTCATTCGCGTACCCGa                                    <  1:4072663/149‑1 (MQ=255)
                                                                                                                    cAGCGAGGGGTTTCGAGCACGCCGGCGCCGCCAAC‑GGG‑CGGCGCCGGCTCACAAAGAAACAAGGACCAAAAGTATTTGTTTTTCGCGCCGCACAGCGGTGGTGGCACGTAAAAGGAGAGGAATGTGAGCGAACTCATTCGCGTACCCGAc                                   >  2:1141343/1‑150 (MQ=255)
                                                                                                                     cgCGGGGGGTTACGAGCACGCCGGCGCCGCCACC‑GGG‑CGGCGCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAGCGGTGGTGGCACGTAAAAGGAGAGGAATGTGAGCGAACTCATTCGCGTACCCGACa                                  <  2:3279819/149‑1 (MQ=255)
                                                                                                                      gcgcGGGGTTTCGCCCCCGCCGGCGCCGCCACC‑GGG‑CGGCCCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAGCGGTGGTGGCACGTAAAAGGAGAGGAATGTGAGCGAACTCATTCGCGTACCCGACAt                                 <  2:5889454/150‑1 (MQ=255)
                                                                                                                        gtgggtTTTCGACCACGCGGGCGCCCCCACC‑GGG‑CGGCCCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAGCGGTGGTGGCACGTAAAAGGAGAGGAATGTGAGCGAACTCATTCGCGTACCCGACATCg                               <  1:4974459/148‑1 (MQ=255)
                                                                                                                        gAGGGGTTCCGAGCACGCGGGCGCCGCACCG‑GGG‑GGGCGCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAGCGGTGGTGGCACGTAAAAGGAGAGGAATGTGAGCGAACTCATTCGCGTACCCGACATCg                               <  2:1813628/150‑1 (MQ=255)
                                                                                                                         cggggTTTCGCCCACGCCGCCGCCGCCCAC‑GGG‑CGGCGCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAGCGGTGGTGGCACGTAAAAGGAGAGGAATGTGAGCGAACTCATTCGCGTACCCGACATCgg                              <  2:8014772/149‑1 (MQ=255)
                                                                                                                         cggggTTGCGCCCACGCCGGCCCCGCCACG‑GGG‑GGGCGCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAGCGGTGGTGGCACGTAAAAGGAGAGGAATGTGAGCGAACTCATTCGCGTACCCGACATCgg                              <  1:8477629/149‑1 (MQ=255)
                                                                                                                          ggggTTGCGAGCCCGCCGGCGCCCCCACC‑GGG‑CGGCGCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAGCGGTGGTGGCACGTAAAAGGAGAGGAATGTGAGCGAACTCATTCGCGTACCCGACATCGGc                             <  2:2288605/150‑1 (MQ=255)
                                                                                                                            ggTTTCGACCACGCCGGCGCCGCCAAC‑GGG‑CGGCGCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAGCGGTGGTGGCACGTAAAAGGAGAGGAATGTGAGCGAACTCATTCGCGTACCCGACATCGGCaa                           <  2:7553103/150‑1 (MQ=255)
                                                                                                                            ggTTCCGACCCCGCCGCCCCCCCCACG‑GGG‑CGCCCCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAGCGGTGGTGGCACGTAAAAGGAGAGGAATGTGAGCGAACTCATTCGCGTACCCGACATCGGCaa                           <  1:8996309/150‑1 (MQ=255)
                                                                                                                             gTTTCGAGCACGCCGGCGCCGCCAAC‑GGG‑CGGCGCCGGCTCACAACGAACCAAGGACCCAAAGTCTTTGTTTTGCGCGCCGCCCAGCGGGGGTGGCACGGAAAAGGAGAGGAATGTGAGCGAACGCATTCGCGTACCCGACCTCGGCCAc                          >  2:6700450/1‑150 (MQ=255)
                                                                                                                              tttCGAGCACGCCGGGGCCGCCAAC‑GGG‑CGGCGCCGGCTCACAACGAACCAACGACCAAAAGTCTTTGTTTTTCGCGCCCCCCAGCGGTGGGGGCACGTAAAAGGAGAGGTAAGTGTGCGACCACAATCGCGTACCCGACATCGGCAACg                         >  2:3605688/1‑150 (MQ=255)
                                                                                                                               ttCGACCACGCCGGCGCCGCCACG‑GGG‑GGGGGCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAGCGGTGGTGGCACGTAAAAGGAGAGGAATGTGAGCGAACTCATTCGCGTACCCGACATCGGCAACgg                        <  2:8760082/150‑1 (MQ=255)
                                                                                                                                 cGAGCACGCCGGCGCCGCCAAC‑GGG‑CGGCGCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAGCGGTGGTGGCACGTAAAAGGAGAGGAATGTGAGCGAACTCATTCGCGTACCCGACATCGGCAACGGTg                      <  2:2139030/150‑1 (MQ=255)
                                                                                                                                  gcgcccGCCGGC‑CCCCCAACGGGG‑CGGCGCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAGCGGTGGTGGCACGTAAAAGGAGAGGAATGTGAGCGAACTCATTCGCGTACCCGACATCGGCAACGGTGa                     <  1:457134/145‑1 (MQ=255)
                                                                                                                                  gaccACGCGGCCGCCCCCACC‑GGG‑CGGCGCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAGCGGTGGTGGCACGTAAAAGGAGAGGAATGTGAGCGAACTCATTCGCGTACCCGACATCGGCAACGGTGa                     <  2:9808517/147‑1 (MQ=255)
                                                                                                                                  cccccccccGGCCCCCCCACC‑GGC‑GGGCCCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAGCGGTGGTGGCACGTAAAAGGAGAGGAATGTGAGCGAACTCATTCGCGTACCCGACATCGGCAACGGTGa                     <  1:3567999/143‑1 (MQ=255)
                                                                                                                                   cccccGCCGGCCCCGCCACC‑GGC‑CGGCGCCGGCTCACACCGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAGCGGTGGTGGCACGTAAAAGGAGAGGAATGTGAGCGAACTCATTCGCGTACCCGACATCGGCAACGGTGAg                    <  1:2743707/146‑1 (MQ=255)
                                                                                                                                   cccACGCGGGCGCCGCCACC‑GGG‑GGGCCCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAGCGGTGGTGGCACGTAAAAGGAGAGGAATGTGAGCGAACTCATTCGCGTACCCGACATCGGCAACGGTGAg                    <  1:175763/148‑1 (MQ=255)
                                                                                                                                        gcgggCGCCGCCATC‑GGG‑GGGCGCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAGCGGTGGTGGCACGTAAAAGGAGAGGAATGTGAGCGAACTCATTCGCGTACCCGACATCGGCAACGGTGAGGGTGa               <  2:3065314/147‑1 (MQ=255)
                                                                                                                                          ccgcgcCGCCAAC‑GCG‑CGGCGCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAGCGGTGGTGGCACGTAAAAGGAGAGGAATGTGAGCGAACTCATTCGCGTACCCGACATCTGCAACGGTGAGGGTGAAg             <  2:1300510/148‑1 (MQ=255)
                                                                                                                                          cGGCGCCGCCAAC‑GGG‑CGGCGCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAGCGGTGGTGGCACGTAAAAGGAGAGGAATGTGAGCGAACTCATTCGCGTACCCGACATCGGCAACGGTGAGGGTGAAg             >  2:8245473/1‑150 (MQ=255)
                                                                                                                                           ggCGCCGCCAAC‑GGG‑CGGCGCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAGCGGTGGTGGCACGTAAAAGGAGAGGAATGTGAGCGAACTCATTCGCGTACCCGACATCGGCCACGGTGGGGGGGAAgg            >  2:10689741/1‑149 (MQ=255)
                                                                                                                                            gcgcCGCCAAC‑GGG‑CGGCGCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAGCGGTGGTGGCACGTACAAGGAGAGGAATGTGAGCGAACTCATTCGCGTACCCGACATCGGCAACGGTGAGGGTGAAGTc           >  1:94450/1‑150 (MQ=255)
                                                                                                                                               ccgccAAC‑GGG‑CGGCGCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAGCGGTGGTGGCACGTAAAAGGAGAGGAATGTGAGCGAACTCATTCGCGTACCCGACATCGGCAACGGTGAGGGTGAAGTCATc        >  2:4549871/1‑150 (MQ=255)
                                                                                                                                                  ccACC‑GGG‑CGGCGCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAGCGGTGGTGGCACGTAAAAGGAGAGGAATGTGAGCGAACTCATTCGCGTACCCGACATCGGCAACGGTGAGGGTGAAGTCATCGAg     <  2:8112740/150‑1 (MQ=255)
                                                                                                                                                   cAAC‑GGG‑CGGCGCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAGCGGTGGTGGCACGTAAAAGGAGAGGAATGTGAGCGAACTCATTCGCGTACCCGACATCGGCAACGGTGAGGGTGAAGTCATCGAgc    >  1:10052985/1‑150 (MQ=255)
                                                                                                                                                    aaC‑GGG‑CGGCGCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAGCGGTGGTGGCACGTAAAAGGAGAGGAATGTGAGCGAACTCATTCGCGTACCCGACATCGGCAACGGTGAGGGTGAAGTCATCGAgct   >  1:5433037/1‑150 (MQ=255)
                                                                                                                                                     aC‑GGGCCGGCCCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAGCGGTGGTGGCACGTAAAAGGAGAGGAATGTGAGCGAACTCATTCGCGTACCCGACATCGGCAACGGTGAGGGTGAAGTCATCGAgct   <  1:3698708/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                       
TGGCCGATCGCGGCGATATCGAGCCGAAAGTGGTGGCCGAGGCCATCGCCAAGTACGGTATCGATCCGGAGAAGCGCAACCCGCTGGATTGCTAATTCGCTCCATACTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGCCGCCAAC‑GGG‑CGGCGCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAGCGGTGGTGGCACGTAAAAGGAGAGGAATGTGAGCGAACTCATTCGCGTACCCGACATCGGCAACGGTGAGGGTGAAGTCATCGAGCTG  >  NC_008463/5910107‑5910405

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑