Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 2,840,442 T→C intergenic (+42/‑47) PA14_RS13335 → / → PA14_RS13340 LysR substrate‑binding domain‑containing protein/sigma‑70 family RNA polymerase sigma factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084632,840,4420TC100.0% 1799.0 / NA 453intergenic (+42/‑47)PA14_RS13335/PA14_RS13340LysR substrate‑binding domain‑containing protein/sigma‑70 family RNA polymerase sigma factor
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (218/235);  total (218/235)

Only 100 of 453 total aligned reads displayed.

CGGCGAGGGCGCGGTGGGTATGTGCTGGCGCGACGAACCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTTTTGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCATGGCTGGTTGCAGAACTGGTTGCGCGGCAAGCTG  >  NC_008463/2840298‑2840587
                                                                                                                                                |                                                                                                                                                 
  gCGAGGGCGCGGTGGGTATGTGCTGGCGCGACGAACCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACaa                                                                                                                                            >  1:8328597/1‑150 (MQ=255)
    gAGGGCGCGGGGGGTATGTGCTGGCGCGTCGAACCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATg                                                                                                                                          <  2:5035886/150‑1 (MQ=255)
     aGGGCGCGGGGGGTATGTGCTGGCGCGACGAACCGCATCGCGACCGCGCCAGCGACGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTCGGGCGCCGGCCGGCGGGTGCTCTTGCAAAAAGGCCGCGGCTTCTCGACAATGc                                                                                                                                         >  2:5348425/1‑150 (MQ=255)
       ggCGCGGTGGGTATGTGCTGGCGCGACGAACCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGa                                                                                                                                       >  1:9361184/1‑150 (MQ=255)
       ggCGCGGTGGGTATGTGCTGGCGCGACGAACCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGa                                                                                                                                       <  1:2346514/150‑1 (MQ=255)
        gcgcGGTGGGTATGTGCTGGCGCGACGAACCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGAt                                                                                                                                      <  1:10053314/150‑1 (MQ=255)
          gcgGTGGGTATGTGCTGGCGCGACGAACCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTc                                                                                                                                    <  1:3493085/150‑1 (MQ=255)
          gcgGTGGGTATGTGCGGGCGCGACGAACCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTc                                                                                                                                    <  2:7251543/150‑1 (MQ=255)
             gTGGGTATGTGCTGGCGCGACGAACCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCAtt                                                                                                                                 >  1:10145097/1‑150 (MQ=255)
             gTGGGTATGTGCTGGCGCGACGAACCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCAtt                                                                                                                                 <  2:4474890/150‑1 (MQ=255)
              tGGGTATGTGCTGGCGCGCCGACCCGCATCGCGCCCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCAGTa                                                                                                                                <  2:7671977/150‑1 (MQ=255)
              tGGGTATGTGCTGGCGCGACGAACCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTa                                                                                                                                <  1:1831130/150‑1 (MQ=255)
               gggTATGTGCTGGCGCGACGAACCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTAt                                                                                                                               >  2:6600018/1‑150 (MQ=255)
               gggTATGTGCTGGCGCGACGAACCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTAt                                                                                                                               <  2:30392/150‑1 (MQ=255)
                ggTATGTGCTGGCGCGACGAACCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTAtc                                                                                                                              >  2:480648/1‑150 (MQ=255)
                   aTGTGCTGGCGCGACGAACCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACACTGCGATTCATTCtcatt                                                                                                                           >  2:10611932/1‑150 (MQ=255)
                   aTGTGCTGGCGCGACGAACCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTAtcatt                                                                                                                           <  2:7752866/150‑1 (MQ=255)
                   aTGTGCTGGCGCGACGAACCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTAtcatt                                                                                                                           >  1:1305605/1‑150 (MQ=255)
                      tgCTGGCGCGACGAACCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTAtcattcat                                                                                                                        <  1:2107097/150‑1 (MQ=255)
                        cTGGCGCGACGAACCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCAtgt                                                                                                                      <  2:8360824/150‑1 (MQ=255)
                          ggCGCGACGAACCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATgtgt                                                                                                                    >  2:4665591/1‑150 (MQ=255)
                          ggCGCGACGAACCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATgtgt                                                                                                                    <  2:5206199/150‑1 (MQ=255)
                          ggCGCGACGAACCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATgtgt                                                                                                                    >  2:1916155/1‑150 (MQ=255)
                             gcgACGAACCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGc                                                                                                                 >  1:3650504/1‑150 (MQ=255)
                                acgaACCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAATAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGcccc                                                                                                              >  2:6836888/1‑150 (MQ=255)
                                acgaACCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGcccc                                                                                                              >  1:3760692/1‑150 (MQ=255)
                                 cgaACCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCg                                                                                                             >  1:5725827/1‑150 (MQ=255)
                                   aaCCGCATCGCGCCCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGAc                                                                                                           <  1:6955752/150‑1 (MQ=255)
                                   aaCCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGAc                                                                                                           >  2:7989350/1‑150 (MQ=255)
                                   aaCCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGAc                                                                                                           >  2:989510/1‑150 (MQ=255)
                                   aaCCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGAc                                                                                                           <  1:8111550/150‑1 (MQ=255)
                                    aCCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGTCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACt                                                                                                          <  1:8664694/150‑1 (MQ=255)
                                         aTCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTccc                                                                                                     <  2:9730076/150‑1 (MQ=255)
                                          tCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCa                                                                                                    >  1:5048203/1‑150 (MQ=255)
                                          tCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCa                                                                                                    <  1:3075479/150‑1 (MQ=255)
                                            gcgACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATg                                                                                                  <  2:9520544/150‑1 (MQ=255)
                                            gcgACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATg                                                                                                  <  1:9083486/150‑1 (MQ=255)
                                              gACCGCGCCCGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTCTTGCAAAATGGCCGCGGCTTCTCGACACTGCGATTCATTGTCATTCATGTGTCGCCCCGACTTTCCCATGTc                                                                                                >  2:1383189/1‑150 (MQ=255)
                                              gACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTc                                                                                                <  1:4259986/150‑1 (MQ=255)
                                               aCCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTcc                                                                                               <  1:10451154/150‑1 (MQ=255)
                                               aCCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTcc                                                                                               <  2:3707837/150‑1 (MQ=255)
                                                ccGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCg                                                                                              >  1:4838721/1‑150 (MQ=255)
                                                 cgcgCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGc                                                                                             >  2:9057586/1‑150 (MQ=255)
                                                   cgcCTGCGAAGACGTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGTGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGccc                                                                                           <  2:7829369/150‑1 (MQ=255)
                                                    gcCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGcccc                                                                                          <  2:1113320/150‑1 (MQ=255)
                                                      cAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGa                                                                                        <  1:6707550/150‑1 (MQ=255)
                                                      cAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGa                                                                                        >  2:6454019/1‑150 (MQ=255)
                                                             gACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTtct                                                                                 >  1:7650907/1‑150 (MQ=255)
                                                                   ctgGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAgcgc                                                                           <  1:3965965/150‑1 (MQ=255)
                                                                   ctgGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCAGTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAgcgc                                                                           <  2:10469506/150‑1 (MQ=255)
                                                                   ctgGCCAGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCCgcgc                                                                           <  1:259324/150‑1 (MQ=255)
                                                                    tgGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGcc                                                                          >  1:6175705/1‑150 (MQ=255)
                                                                      gcctgcctGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCcca                                                                        <  2:7401559/150‑1 (MQ=255)
                                                                         tgcctgcGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCccacca                                                                     <  1:9928329/150‑1 (MQ=255)
                                                                          gcctgcGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCAt                                                                    <  1:2543298/150‑1 (MQ=255)
                                                                           cctgcGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATc                                                                   >  1:5426926/1‑150 (MQ=255)
                                                                            ctgcGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATcg                                                                  <  1:8843764/150‑1 (MQ=255)
                                                                            ctgcGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATcg                                                                  <  2:4197934/150‑1 (MQ=255)
                                                                              gcgcGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATcgcg                                                                >  1:3567982/1‑150 (MQ=255)
                                                                                 cgAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGAGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCca                                                             <  2:8548849/150‑1 (MQ=255)
                                                                                   aaGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCcatg                                                           >  2:3338290/1‑150 (MQ=255)
                                                                                      gcgcGGCGGCCGAGGAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAAGGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCcatgcat                                                        <  1:2801044/150‑1 (MQ=255)
                                                                                        gcggcgGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCatgcatgc                                                      <  1:7259651/150‑1 (MQ=255)
                                                                                        gcggcgGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCatgcatgc                                                      <  2:6921909/150‑1 (MQ=255)
                                                                                        gcggcgGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCatgcatgc                                                      <  2:5797727/150‑1 (MQ=255)
                                                                                         cggcggCCGTGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATgcg                                                     <  2:3652205/150‑1 (MQ=255)
                                                                                         cggcggCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGAGTCAGTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCGTCTCAGCGCCCACCATCGCGCCATGCATgcg                                                     >  1:8515660/1‑150 (MQ=255)
                                                                                               ccGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCCGCGCCCACCATCGCGCCATGCATGCGCTGTAc                                               <  2:4727658/150‑1 (MQ=255)
                                                                                                 gAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAg                                             <  1:869778/150‑1 (MQ=255)
                                                                                                 gAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAg                                             <  1:9777378/150‑1 (MQ=255)
                                                                                                  aGTAGGGCGCCGGCCGGCGGGTGCGATTGCAAAAAGGCCGCGGCGTCTCGACAATGCGATTCATTATCATTCATGTTTCGCCCCGACTGGCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGc                                            <  1:9522341/150‑1 (MQ=255)
                                                                                                   gTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCTTGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCg                                           >  1:3359900/1‑150 (MQ=255)
                                                                                                      gggCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGc                                        >  2:3429551/1‑150 (MQ=255)
                                                                                                         cgcCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCAcc                                     >  1:5297901/1‑150 (MQ=255)
                                                                                                          gccggccgGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCa                                    >  1:8414713/1‑150 (MQ=255)
                                                                                                           ccggccggCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCAt                                   >  2:408574/1‑150 (MQ=255)
                                                                                                            cggccggcGGGTGCTATTGCAAACAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCATg                                  <  2:6752847/150‑1 (MQ=255)
                                                                                                                  gcggGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCATGGCTGGt                            >  2:10154753/1‑150 (MQ=255)
                                                                                                                   cggGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCATGGCTGGtt                           >  1:1986488/1‑150 (MQ=255)
                                                                                                                    gggTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCATGGCTGGTTg                          <  1:4058665/150‑1 (MQ=255)
                                                                                                                    gggTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCATGGCTGGTTg                          <  1:9457739/150‑1 (MQ=255)
                                                                                                                    gggAGCTATAGCACAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATACCTGTGTCGCCCCGACTGTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCATGGCTGGTTg                          <  2:9138983/150‑1 (MQ=255)
                                                                                                                     ggTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCATGGCTGGTTGc                         >  1:4463912/1‑150 (MQ=255)
                                                                                                                        gCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCATGGCTGGTTGCAGa                      >  2:8959059/1‑150 (MQ=255)
                                                                                                                            ttGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCATGGCTGGTTGCAGAACTg                  >  2:5456843/1‑150 (MQ=255)
                                                                                                                            ttGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCATGGCTGGTTGCAGAACTg                  >  2:9219438/1‑150 (MQ=255)
                                                                                                                             tGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCATGGCTGGTTGCAGAACTgg                 <  1:7551966/150‑1 (MQ=255)
                                                                                                                                aaaaaGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCATGGCTGGTTGCAGAACTGGTTg              >  2:2350585/1‑150 (MQ=255)
                                                                                                                                 aaaaGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCATGGCTGGTTGCAGAACTGGTTgc             >  2:167595/1‑150 (MQ=255)
                                                                                                                                  aaaGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCATGGCTGGTTGCAGAACTGGTTgcg            >  2:5560624/1‑150 (MQ=255)
                                                                                                                                   aaGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCATGGCTGGTTGCAGAACTGGTTgcgc           <  1:8585830/150‑1 (MQ=255)
                                                                                                                                   aaGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCATGGCTGGTTGCAGAACTGGTTgcgc           <  2:2155860/150‑1 (MQ=255)
                                                                                                                                   aaGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCATGGCTGGTTGCAGAACTGGTTgcgc           <  2:6099043/150‑1 (MQ=255)
                                                                                                                                    aGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCATGGCTGGTTGCAGAACTGGTTGcgcg          >  1:142831/1‑150 (MQ=255)
                                                                                                                                    aGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCATGGCTGGTTGCAGAACTGGTTGcgcg          >  2:2754025/1‑150 (MQ=255)
                                                                                                                                    aGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCGGTACAGCGAGCACCATGGCTGGTTGCAGAACTGGGTGcgcg          >  1:7921788/1‑150 (MQ=255)
                                                                                                                                     ggCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCATGGCTGGTTGCAGAACTGGTTGCGCgg         >  2:1336806/1‑150 (MQ=255)
                                                                                                                                       ccGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCATGGCTGGTTGCAGAACTGGTTGCGCGGCa       <  1:10643795/150‑1 (MQ=255)
                                                                                                                                         gcgGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCATGGCTGGTTGCAGAACTGGTTGCGCGGCAAg     >  1:5977244/1‑150 (MQ=255)
                                                                                                                                         gcgGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCGTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCATGGCTGGTTGCAGAACTGGTTGCGCGGCAAg     >  1:6466441/1‑150 (MQ=255)
                                                                                                                                                |                                                                                                                                                 
CGGCGAGGGCGCGGTGGGTATGTGCTGGCGCGACGAACCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTTTTGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCATGGCTGGTTGCAGAACTGGTTGCGCGGCAAGCTG  >  NC_008463/2840298‑2840587

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑