Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 2,955,357 A→G L314L (TTG→CTG)  PA14_RS13700 ← PepSY domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084632,955,3570AG100.0% 1708.3 / NA 429L314L (TTG→CTG) PA14_RS13700PepSY domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (218/211);  total (218/211)

Only 100 of 429 total aligned reads displayed.

AGATCAGCAGGCCGCTGAGCACCATCATGCTCAGCAGCAGGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAAGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCAGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAAGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCC  >  NC_008463/2955210‑2955503
                                                                                                                                                   |                                                                                                                                                  
  aTCAGCAGGCCGCTGAGCACCATCATGCTCAGCAGCAGGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCgg                                                                                                                                                >  1:2544614/1‑150 (MQ=255)
   tCAGCAGGCCGCTGAGCACCATCATGCTCAGCAGCAGGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCggg                                                                                                                                               >  2:1610845/1‑150 (MQ=255)
    cagcagGCCGCTGAGCACCATCATGCTCAGCAGCAGGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGggt                                                                                                                                              >  1:3329321/1‑150 (MQ=255)
      gcagGCCGCTGAGCACCATCATGCTCAGCAGCAGGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGggtgg                                                                                                                                            >  2:8308685/1‑150 (MQ=255)
      ccagGCCGCTGAGCACCCTCCTGCTCAGCAGCAGGCCGAAGCCGACCCAGATCAGCTTGACCCAGCTCCCGCCGACAGCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGCACTGCAGGGGGGTACGGTCGCTGCGCAGGCGggtgg                                                                                                                                            <  2:5216114/149‑1 (MQ=255)
         ggCCGCTGAGCACCATCATGCTCAGCAGCAGGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGttt                                                                                                                                         <  1:7089365/150‑1 (MQ=255)
         ggCCGCTGAGCACCATCATGCTAAGCAGCAGGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGttt                                                                                                                                         <  1:4284932/150‑1 (MQ=255)
            cGCTGAGCACCATCATGCTCAGCAGCAGGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGa                                                                                                                                      <  1:1175396/150‑1 (MQ=255)
               tGAGCACCATCATGCTCAGCAGCAGGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCt                                                                                                                                   <  1:9290253/150‑1 (MQ=255)
               tGAGCACCATCATGCTCAGCAGCAGGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCt                                                                                                                                   <  1:3423344/150‑1 (MQ=255)
                gAGCACCATCATGCTCAGCAGCAGGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCtt                                                                                                                                  <  1:3729696/150‑1 (MQ=255)
                  gCACCATCATGCTCAGCAGCAGGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACTGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGc                                                                                                                                >  1:5899607/1‑150 (MQ=255)
                   cACCATCATGCTCAGCAGCAGGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGcc                                                                                                                               <  1:7412255/150‑1 (MQ=255)
                       atcatGCTCAGCAGCAGGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGta                                                                                                                           <  2:3063228/150‑1 (MQ=255)
                           tGCTCAGCAGCAGGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGACCTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAg                                                                                                                       >  2:1388365/1‑150 (MQ=255)
                            gCTCAGCAGCAGGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAgg                                                                                                                      <  1:452898/150‑1 (MQ=255)
                            gCTCAGCAGCAGGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAgg                                                                                                                      <  2:6751811/150‑1 (MQ=255)
                              tCAGCAGCAGGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGt                                                                                                                    >  1:10461354/1‑150 (MQ=255)
                               cagcagcagGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGtt                                                                                                                   <  2:4137326/150‑1 (MQ=255)
                                   agcagGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAgc                                                                                                               >  2:7006203/1‑150 (MQ=255)
                                   agcagGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAgc                                                                                                               <  1:1140483/150‑1 (MQ=255)
                                    gcagGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAgcg                                                                                                              >  1:6296442/1‑150 (MQ=255)
                                        gCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGc                                                                                                          >  1:9850728/1‑150 (MQ=255)
                                        gCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGc                                                                                                          <  1:3476385/150‑1 (MQ=255)
                                        gCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGc                                                                                                          >  2:4866862/1‑150 (MQ=255)
                                          cGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCg                                                                                                        <  1:7616227/150‑1 (MQ=255)
                                          cGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCg                                                                                                        >  2:1130280/1‑150 (MQ=255)
                                           gAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGa                                                                                                       >  2:925389/1‑150 (MQ=255)
                                                cGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGa                                                                                                  >  2:6430261/1‑150 (MQ=255)
                                                cGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGa                                                                                                  >  1:8818931/1‑150 (MQ=255)
                                                   aCCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACa                                                                                               >  2:1885515/1‑150 (MQ=255)
                                                   aCCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACa                                                                                               <  1:5469859/150‑1 (MQ=255)
                                                   aCCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACa                                                                                               <  2:10349521/150‑1 (MQ=255)
                                                      aGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGa                                                                                            <  2:10669945/150‑1 (MQ=255)
                                                       gATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAg                                                                                           >  2:4521973/1‑150 (MQ=255)
                                                          cAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCgg                                                                                        <  2:3149559/150‑1 (MQ=255)
                                                            gCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGAt                                                                                      <  1:4069935/150‑1 (MQ=255)
                                                             cTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATa                                                                                     <  1:4559178/150‑1 (MQ=255)
                                                             cTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATa                                                                                     <  2:7622831/150‑1 (MQ=255)
                                                              ttGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATAc                                                                                    >  1:6041375/1‑150 (MQ=255)
                                                              ttGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATAc                                                                                    >  1:8205753/1‑150 (MQ=255)
                                                              ttGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATAc                                                                                    <  2:3396155/150‑1 (MQ=255)
                                                               tGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATAcc                                                                                   >  2:4972661/1‑150 (MQ=255)
                                                                  cccAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCCGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGc                                                                                >  2:7976599/1‑150 (MQ=255)
                                                                  cccAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGc                                                                                <  1:10223455/150‑1 (MQ=255)
                                                                   ccAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGcc                                                                               <  1:2883638/150‑1 (MQ=255)
                                                                     aGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCcgc                                                                             <  1:3887766/150‑1 (MQ=255)
                                                                     aGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCcgc                                                                             >  2:908691/1‑150 (MQ=255)
                                                                        tACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTc                                                                          >  2:10078494/1‑150 (MQ=255)
                                                                          ccgccgAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCTGCCGACTGGAACATGAGCGGATACCAGCCGCGTccg                                                                        >  1:6767055/1‑149 (MQ=255)
                                                                          ccgccgAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTccg                                                                        >  2:6088632/1‑149 (MQ=255)
                                                                          ccgccgAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTccg                                                                        >  1:163316/1‑149 (MQ=255)
                                                                             ccgAACTCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGcc                                                                     <  2:3667423/150‑1 (MQ=255)
                                                                              cgAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCa                                                                    <  1:10321133/150‑1 (MQ=255)
                                                                                 aaTCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGa                                                                 >  1:187082/1‑150 (MQ=255)
                                                                                  aTCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGaa                                                                >  2:569410/1‑150 (MQ=255)
                                                                                  aTCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGaa                                                                <  1:892729/150‑1 (MQ=255)
                                                                                      ccGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATc                                                            >  1:2378279/1‑150 (MQ=255)
                                                                                        ggTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCgg                                                          <  2:7859623/150‑1 (MQ=255)
                                                                                          tgtgCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGc                                                        <  2:9865650/150‑1 (MQ=255)
                                                                                          tgtgCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGc                                                        <  2:8668636/150‑1 (MQ=255)
                                                                                          tgtgCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGc                                                        >  2:5409840/1‑150 (MQ=255)
                                                                                           gtgCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCt                                                       <  2:2443408/150‑1 (MQ=255)
                                                                                            tgCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTg                                                      >  1:9481041/1‑150 (MQ=255)
                                                                                            tgCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTg                                                      >  1:7883058/1‑150 (MQ=255)
                                                                                             gCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGt                                                     <  1:10050399/150‑1 (MQ=255)
                                                                                                ggggACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAgg                                                  <  2:9528523/150‑1 (MQ=255)
                                                                                                ggggACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAgg                                                  <  2:2726431/150‑1 (MQ=255)
                                                                                                  ggACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCg                                                >  2:617335/1‑150 (MQ=255)
                                                                                                     cGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTg                                             >  1:5112448/1‑150 (MQ=255)
                                                                                                       cATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGcc                                           >  1:1272425/1‑150 (MQ=255)
                                                                                                       cATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGcc                                           >  2:8433380/1‑150 (MQ=255)
                                                                                                         tGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCgg                                         >  1:8475804/1‑150 (MQ=255)
                                                                                                         tGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCgg                                         >  1:9066517/1‑150 (MQ=255)
                                                                                                          ggATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCggg                                        <  1:10499826/150‑1 (MQ=255)
                                                                                                             ttCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCag                                     >  1:2795422/1‑149 (MQ=255)
                                                                                                              tCGGTGACGAACTGCAGGGGGGGACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCagg                                    <  2:8824771/150‑3 (MQ=255)
                                                                                                                   gACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAacac                               <  2:1241524/150‑1 (MQ=255)
                                                                                                                    aCGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACAcc                              >  2:941323/1‑150 (MQ=255)
                                                                                                                      gAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACcgc                            >  1:49174/1‑150 (MQ=255)
                                                                                                                      gAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACcgc                            <  2:7247856/150‑1 (MQ=255)
                                                                                                                       aaCTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACcgcg                           <  1:2945876/150‑1 (MQ=255)
                                                                                                                        aCTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCgcgc                          <  1:5325282/150‑1 (MQ=255)
                                                                                                                         cTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGTGCAGGAACACCGCGCt                         <  2:4383729/150‑1 (MQ=255)
                                                                                                                         cTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCt                         >  1:2108716/1‑150 (MQ=255)
                                                                                                                          tGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTg                        <  1:3104523/150‑1 (MQ=255)
                                                                                                                             aGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGcc                     >  1:3648742/1‑150 (MQ=255)
                                                                                                                              ggggggTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCCCGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCt                    >  2:1326513/1‑150 (MQ=255)
                                                                                                                              ggggggTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCt                    >  1:1720614/1‑150 (MQ=255)
                                                                                                                              ggggggTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCt                    <  2:3564623/150‑1 (MQ=255)
                                                                                                                                 gggTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCa                 <  2:3932272/150‑1 (MQ=255)
                                                                                                                                 gggCACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCa                 >  2:17161/1‑150 (MQ=255)
                                                                                                                                    tACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGc              >  1:5832289/1‑150 (MQ=255)
                                                                                                                                        gTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTggg          >  1:4150321/1‑150 (MQ=255)
                                                                                                                                           gCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGAt       >  1:1408031/1‑150 (MQ=255)
                                                                                                                                           gCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGAt       >  1:7229933/1‑150 (MQ=255)
                                                                                                                                             tGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATAc     >  1:5689576/1‑150 (MQ=255)
                                                                                                                                             tGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATAc     >  2:2143098/1‑150 (MQ=255)
                                                                                                                                              gAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACg    >  2:9649172/1‑150 (MQ=255)
                                                                                                                                               aGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGc   >  1:4739646/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
AGATCAGCAGGCCGCTGAGCACCATCATGCTCAGCAGCAGGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAAGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCAGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAAGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCC  >  NC_008463/2955210‑2955503

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑