Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 3,051,413 T→C K369E (AAG→GAG)  PA14_RS13990 ← SfnB family sulfur acquisition oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084633,051,4130TC100.0% 1928.3 / NA 486K369E (AAG→GAG) PA14_RS13990SfnB family sulfur acquisition oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (258/228);  total (258/228)

Only 100 of 486 total aligned reads displayed.

GCTCATCGGGCGCTCCTCAGATCCATGAGTGACGCGCCGGACGGGCGCCGTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTTGAGGTTGAACTTGGCGAGCGTGGCGCGGCTGCCGGCCAGCTTGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTCGGCGTCCACCGGCCGCGCGGCCACTTCGT  >  NC_008463/3051266‑3051559
                                                                                                                                                   |                                                                                                                                                  
   cATCGGGCGCTCCTCAGATCCATGAGTGACGCGCCGGACGGGCGCCGTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGa                                                                                                                                               >  2:8844049/1‑150 (MQ=255)
   cATCGGGCGCTCCTCAGATCCATGAGTGACGCGCCGGACGGGCGCCGTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGa                                                                                                                                               >  2:7878931/1‑150 (MQ=255)
    aTCGGGCGCTCCTCAGATCCATGAGTGACGCGCCGGACGGGCGCCGTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAg                                                                                                                                              >  1:1578219/1‑150 (MQ=255)
         gcgcTCCTCAGATCCATGAGTGACGCGCCGGACGGGCGCCGTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTgg                                                                                                                                         >  1:7908817/1‑150 (MQ=255)
            ctcctcAGATCCATGAGTGACGCGCCGGACGGGCGCCGTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGgcgc                                                                                                                                      <  2:2076003/150‑1 (MQ=255)
              cctcAGATCCATGAGTGACGCGCCGGACGGGCGCCGTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCgg                                                                                                                                    >  2:10260306/1‑150 (MQ=255)
              cctcAGATCCATGAGTGACGCGCCGGACGGGCGCCGTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCgg                                                                                                                                    >  1:6279392/1‑150 (MQ=255)
                 cAGATCCATGAGTGACTCGCCGGACGGGCGCCGTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGAGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTg                                                                                                                                 >  1:55566/1‑150 (MQ=255)
                 cAGATCCATGAGTGACGCGCCGGACGGGCGCCGTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTg                                                                                                                                 <  1:1126421/150‑1 (MQ=255)
                 cAGATCCATGAGTGACGCGCCGGACGGGCGCCGTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTg                                                                                                                                 <  1:4937891/150‑1 (MQ=255)
                  aGATCCATGAGTGACGCGCCGGACGGGCGCCGTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGc                                                                                                                                >  2:9493200/1‑150 (MQ=255)
                   gATCCATGAGTGACGCGCCGGACGGGCGCCGTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGcc                                                                                                                               <  2:1818153/150‑1 (MQ=255)
                   gATCCATGAGTGACGCGCCGGACGGGCGCCGTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGcc                                                                                                                               >  2:3025312/1‑150 (MQ=255)
                      ccATGAGTGACGCGCCGGACGGGCGCCGTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGc                                                                                                                            <  2:5751822/150‑1 (MQ=255)
                      ccATGAGTGACGCGCCGGACGGGCGCCGTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGc                                                                                                                            <  1:269065/150‑1 (MQ=255)
                      ccATGAGTGACGCGCCGGACGGGCGCCGTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGc                                                                                                                            <  1:2300319/150‑1 (MQ=255)
                       cATGAGTGACGCGCCGGACGGGCGCCGTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGTGCGTGGCGCGGCTGCCGGcc                                                                                                                           <  2:6904980/150‑1 (MQ=255)
                       cATGAGTGACGCGCCGGACGGGCGCCGTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGcc                                                                                                                           <  1:10205112/150‑1 (MQ=255)
                       cATGAGTGACGCGCCGGACGGGCGCCGTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGcc                                                                                                                           >  1:243367/1‑150 (MQ=255)
                        aTGAGTGACGCGCCGGACGGGCGCCGTTCAGGTAGTCGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTAGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCa                                                                                                                          <  1:6768180/150‑1 (MQ=255)
                         tGAGTGACGCGCCGGACGGGCGCCGTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAg                                                                                                                         >  1:3914411/1‑150 (MQ=255)
                          gAGTGACGCGCCGGACGGGCGCCGTTCAGGTAGTAGTCGCCGACCGCGTGNACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGc                                                                                                                        >  1:5629134/1‑150 (MQ=255)
                          gAGTGACGCGCCGGACGGGCGCCGTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGc                                                                                                                        >  1:8855316/1‑150 (MQ=255)
                            gTGACGCGCCGGACGGGCGCCGTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCtc                                                                                                                      <  2:6616284/150‑2 (MQ=255)
                              gACGCGCCGGACGGGCGCCGTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGa                                                                                                                    <  2:921183/150‑1 (MQ=255)
                               ccgcgCCGGACGGGCGCCGTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGaa                                                                                                                   <  2:8601030/149‑1 (MQ=255)
                               aCGCGCCGGACGGGCGCCGTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGGGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGaa                                                                                                                   >  1:1858237/1‑150 (MQ=255)
                                 gcgcCGGACGGGCGCCGTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAaga                                                                                                                 >  1:7604938/1‑150 (MQ=255)
                                   gcCGGACGGGCGCCGTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGc                                                                                                               >  1:844026/1‑150 (MQ=255)
                                   gcCGGACGGGCGCCGTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGc                                                                                                               <  2:27684/150‑1 (MQ=255)
                                    ccGGACGGGCGCCGTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCt                                                                                                              >  1:8974493/1‑150 (MQ=255)
                                      ggACGGGCGCCGTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTtc                                                                                                            >  1:5912515/1‑150 (MQ=255)
                                         cGGGCGCCGTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCg                                                                                                         >  1:4805748/1‑150 (MQ=255)
                                         cGGGCGCCGTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCg                                                                                                         <  1:10098372/150‑1 (MQ=255)
                                           ggCGCCGTTCTGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGAAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCTGCTCGAAGAGCTTCTCGCt                                                                                                       <  2:1060444/150‑1 (MQ=25)
                                            gcgcCGTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTg                                                                                                      >  2:147787/1‑150 (MQ=255)
                                            gcgcCGTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTg                                                                                                      >  2:154529/1‑150 (MQ=255)
                                             cgcCGTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTgg                                                                                                     <  2:7525960/150‑1 (MQ=255)
                                               ccGTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGGACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTggcg                                                                                                   <  2:801412/150‑1 (MQ=255)
                                                cGTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTggcgg                                                                                                  >  2:7294148/1‑150 (MQ=255)
                                                  ttCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGcc                                                                                                <  1:6550453/150‑1 (MQ=255)
                                                  ttCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGcc                                                                                                >  1:21564/1‑150 (MQ=255)
                                                   tCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCa                                                                                               >  2:2399842/1‑150 (MQ=255)
                                                   tCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCa                                                                                               >  2:3250322/1‑150 (MQ=255)
                                                        tagtagTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTg                                                                                          >  1:225230/1‑150 (MQ=255)
                                                        tagtagTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTg                                                                                          >  2:6242498/1‑150 (MQ=255)
                                                             gtCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGAtctc                                                                                     >  2:710242/1‑150 (MQ=255)
                                                             gtCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGAtctc                                                                                     <  2:991993/150‑1 (MQ=255)
                                                             gtCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGAtctc                                                                                     >  1:8769583/1‑150 (MQ=255)
                                                              tCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCg                                                                                    >  1:2104826/1‑150 (MQ=255)
                                                                  cGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGc                                                                                >  2:9746168/1‑150 (MQ=255)
                                                                     ccGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCa                                                                             >  2:10091146/1‑150 (MQ=255)
                                                                        cgTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCa                                                                          >  1:4457963/1‑150 (MQ=255)
                                                                        cgTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCa                                                                          >  2:1963546/1‑150 (MQ=255)
                                                                        cgTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCa                                                                          >  1:7227976/1‑150 (MQ=255)
                                                                         gaggACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCAc                                                                         <  2:4239876/148‑1 (MQ=255)
                                                                           ggACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCt                                                                       <  1:5400328/150‑1 (MQ=255)
                                                                            gACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCtt                                                                      <  1:9545466/150‑1 (MQ=255)
                                                                             aCCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTg                                                                     >  2:4054376/1‑150 (MQ=255)
                                                                                 tCCAGCGGACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCt                                                                 <  2:8319098/150‑1 (MQ=255)
                                                                                  ccAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTc                                                                <  1:3093641/150‑1 (MQ=255)
                                                                                  ccAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTc                                                                >  2:5578137/1‑150 (MQ=255)
                                                                                   cAGCGTAGCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCg                                                               <  2:6978077/150‑1 (MQ=255)
                                                                                    aGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCgg                                                              <  1:4675485/150‑1 (MQ=255)
                                                                                       gTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCa                                                           <  2:5470486/150‑1 (MQ=255)
                                                                                        tACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCAc                                                          >  1:8605833/1‑150 (MQ=255)
                                                                                        tACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCAc                                                          <  1:9310756/150‑1 (MQ=255)
                                                                                         aCCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACg                                                         <  1:1541045/150‑1 (MQ=255)
                                                                                             ggTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGa                                                     <  1:2756262/150‑1 (MQ=255)
                                                                                                 gTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGa                                                 <  1:9460076/150‑1 (MQ=255)
                                                                                                 gTGCAGGGTGTGGGAGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGa                                                 <  1:3190367/150‑1 (MQ=255)
                                                                                                  tGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAg                                                >  1:4283645/1‑150 (MQ=255)
                                                                                                     aGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGgcg                                             >  1:3382189/1‑150 (MQ=255)
                                                                                                     aGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGgcg                                             >  1:742126/1‑150 (MQ=255)
                                                                                                      gggTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGgcgc                                            >  2:481096/1‑150 (MQ=255)
                                                                                                         tgtgGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTg                                         <  2:3116841/150‑1 (MQ=255)
                                                                                                           tgGGTGCGGGCGTTGCGCCAGTGCCGGTCGTGGTTGAACTCGGCGTGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCGTGGCCTCGGCGCCGGCGATCGAGGCGCGTgcc                                       >  2:5247202/1‑150 (MQ=38)
                                                                                                           tgGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTgcc                                       <  2:5181584/150‑1 (MQ=255)
                                                                                                              gTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTgccgcc                                    <  1:3571624/150‑1 (MQ=255)
                                                                                                               tGCGGGCGGTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGccgccg                                   <  2:5888022/150‑1 (MQ=255)
                                                                                                                   ggCGTTGCGCCAGTGCCGGTCGAGGTGGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTc                               <  2:3889070/150‑1 (MQ=255)
                                                                                                                     cGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTCgg                             <  1:10372207/150‑1 (MQ=255)
                                                                                                                       ttGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTCGGCg                           <  1:10561448/150‑1 (MQ=255)
                                                                                                                        tGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTCGGCGt                          >  1:4543850/1‑150 (MQ=255)
                                                                                                                         gcgcCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTCGGCGTc                         <  1:686377/150‑1 (MQ=255)
                                                                                                                           gcCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTCGGCGTCCa                       >  1:6476261/1‑150 (MQ=255)
                                                                                                                            ccAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAATAGCTTCTAGCTGGCGGACAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTCGGCGTCAAc                      >  1:296965/1‑150 (MQ=38)
                                                                                                                            ccAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTCGGCGTCCAc                      >  2:10420968/1‑150 (MQ=255)
                                                                                                                             cAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTCGGCGTCCAcc                     >  2:7640914/1‑150 (MQ=255)
                                                                                                                             cAGTGCCGGTCGAGGGTGAACTCGGCGCGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCGTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTCGGCGTCCAcc                     <  2:459827/150‑1 (MQ=39)
                                                                                                                              aGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTCGGCGTCCACCg                    <  1:2030998/150‑1 (MQ=255)
                                                                                                                               gTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTCGGCGTCCACCgg                   >  2:375565/1‑150 (MQ=255)
                                                                                                                                     gtcgaggttgaACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTCGGCGTCCACCGGCcgcgc             <  2:4393010/147‑1 (MQ=255)
                                                                                                                                     gtcgaggttgaACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTCGGCGTCCACCGGCcgcgc             >  2:3650503/4‑150 (MQ=255)
                                                                                                                                     gtcgaggttgaACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTCGGCGTCCACCGGCcgcgc             >  2:1108374/4‑150 (MQ=255)
                                                                                                                                         aggttgaACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTCGGCGTCCACCGGCCGCGCGGcc         >  2:1178084/1‑150 (MQ=255)
                                                                                                                                          ggttgaACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACACGGCGTCCACCGGCCGCGCGGCCa        >  1:10009026/1‑150 (MQ=255)
                                                                                                                                           gttgaACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACGCGGCGTCCACCGGCCGCGCGGCCAc       >  2:6720997/1‑150 (MQ=255)
                                                                                                                                             tgaACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTCGGCGTCCACCGGCCGCGCGGCCACtt     <  2:3497886/150‑1 (MQ=255)
                                                                                                                                             tgaACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTCGGCGTCCACCGGCCGCGCGGCCACtt     <  1:9808614/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GCTCATCGGGCGCTCCTCAGATCCATGAGTGACGCGCCGGACGGGCGCCGTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTTGAGGTTGAACTTGGCGAGCGTGGCGCGGCTGCCGGCCAGCTTGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTCGGCGTCCACCGGCCGCGCGGCCACTTCGT  >  NC_008463/3051266‑3051559

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑