BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_008463	3,387,884	T→C	F48L (TTC→CTC)	cynS →	cyanase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_008463	3,387,884	0	T	C	100.0%	1676.7 / NA	426	F48L (TTC→CTC)	cynS	cyanase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (178/248); total (178/248)

Only 100 of 426 total aligned reads displayed.

CATCCCATGCAACACAGCCAAGTGTCCCCCAACGCGCGCCAGCAACTGGCCGAGACCGTGGTCCTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTATCGCCCTGACATTAGTCACCGCCGCCATGTTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCATGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGCGACCCGACCATCTAC  >  NC_008463/3387737‑3388027

 aTCCCATGCAACACAGCCAAGTGTCCCCCAACGCGCGCCAGCAACTGGCCGAGACCGTGGTCCTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCg                                                                                                                                              <  2:10452727/150‑1 (MQ=255)

  tCCCATGCAACACAGCCAAGTGTCCCCCAACGCGCGCCAGCAACTGGCCGAGACCGTGGTCCTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCgg                                                                                                                                             >  2:6212446/1‑150 (MQ=255)

   cccATGCAACACAGCCAAGTGTCCCCCAACGCGCGCCAGCAACTGGCCGAGACCGTGGTCCTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGc                                                                                                                                            >  2:4857939/1‑150 (MQ=255)

       tGCAACACAGCCAAGTGTCCCCCAACGCGCGCCAGCAACTGGCCGAGACCGTGGTCCTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGc                                                                                                                                        <  2:3579401/150‑1 (MQ=255)

            cacaGCCACGTGTCCCCCAACGCGCGCCAGCAACTGGCCGAGACCGTGGTCCTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGcc                                                                                                                                   <  2:6699846/150‑1 (MQ=255)

              caGCCAAGTGTCCCCCAACGCGCGCCAGCAACTGGCCGAGACCGTGGTCCTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCt                                                                                                                                 <  2:8923883/150‑1 (MQ=255)

                  cAAGTGTCCCCCAACGCGCGCCAGCAACTGGCCGAGACCGTGGTCCTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCa                                                                                                                             >  1:9808930/1‑150 (MQ=255)

                   aaGTGTCCCCCAACGCGCGCCAGCAACTGGCCGAGACCGTGGTCCTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCag                                                                                                                            <  2:533594/150‑1 (MQ=255)

                    aGTGTCCCCCAACGCGCGCCAGCAACTGGCCGAGACCGTGGTCCTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCaga                                                                                                                           <  1:5352937/150‑1 (MQ=255)

                    aGTGTCCCCCAACGCGCGCCAGCAACTGGCCGAGACCGTGGTCCTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCaga                                                                                                                           >  1:5793218/1‑150 (MQ=255)

                    aGTGTCCCCCAACGCGCGCCAGCAACTGGCCGAGACCGTGGTCCTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCaga                                                                                                                           >  1:1892778/1‑150 (MQ=255)

                       gtCCCCCAACGCGCGCCAGCAACTGGCCGAGACCGTGGTCCTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAggc                                                                                                                        >  2:10622254/1‑150 (MQ=255)

                       gtCCCCCAACGCGCGCCAGCAACTGGCCGAGACCGTGGTCCTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAggc                                                                                                                        >  1:5185182/1‑150 (MQ=255)

                         cccccAACGCGCGCCAGCAACTGGCCGAGACCGTGGTCCTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAggcgg                                                                                                                      <  2:6339116/150‑1 (MQ=255)

                         cccccAACGCGCGCCAGCAACTGGCCGAGACCGTGGTCCTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAggcgg                                                                                                                      <  1:3540618/150‑1 (MQ=255)

                          ccccAACGCGCGCCAGCAACTGGCCGAGACCGTGGTCCTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAggcggc                                                                                                                     <  1:9173010/150‑1 (MQ=255)

                              aaCGCGCGCCAGCAACTGGCCGAGACCGTGGTCCTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGgcggcggcg                                                                                                                 >  2:6978995/1‑150 (MQ=255)

                              aaCGCGCGCCAGCAACTGGCCGAGACCGTGGTCCTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGgcggcggcg                                                                                                                 >  1:7981386/1‑150 (MQ=255)

                                 gcgcgcCAGCAACTGGCCGAGACCGTGGTCCTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGgcgcgc                                                                                                              >  2:5675026/1‑150 (MQ=255)

                                 gcgcgcCAGCAACTGGCCGAGACCGTGGTCCTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGgcgcgc                                                                                                              <  2:2377234/150‑1 (MQ=255)

                                  cgcgcCAGCAACTGGCCGAGACCGTGGTCCTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCa                                                                                                             <  2:3526228/150‑1 (MQ=255)

                                   gcgcCAGCAACTGGCCGAGACCGTGGTCCTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCaa                                                                                                            >  2:9509502/1‑150 (MQ=255)

                                     gcCAGCAACTGGCCGAGACCGTGGTCCTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAgg                                                                                                          >  2:4333949/1‑150 (MQ=255)

                                       cAGCAACGGGCCGAGACCGTGGTCCTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTc                                                                                                        <  2:4464471/150‑1 (MQ=255)

                                        aGCAACTGGCCGAGACCGTGGTCCTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTcg                                                                                                       >  1:9512956/1‑150 (MQ=255)

                                           accTGGCCGAGACCGTGGTCCTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTcgccg                                                                                                    <  1:2918603/148‑1 (MQ=255)

                                            aCTGGCCGAGACCGTGGTCCTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGAGCTGGCCGACGGCACCGGGCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCCGAGGCGGCGGCGCGCAAGGTcgccgc                                                                                                   >  2:7876007/1‑150 (MQ=255)

                                             cTGGCCGAGACCGTGGTCCTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCgccgcc                                                                                                  <  2:3800582/150‑1 (MQ=255)

                                              tGGCCGAGACCGTGGTCCTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGccc                                                                                                 <  1:9951693/150‑1 (MQ=255)

                                              tGGCCGAGACCGTGGTCCTGAACAAGGCACGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGccc                                                                                                 >  2:2782458/1‑150 (MQ=255)

                                               ggCCGAGACCGTGGTCCTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCa                                                                                                >  2:2437910/1‑150 (MQ=255)

                                                  cGAGACCGTGGTCCTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACt                                                                                             <  1:8582623/150‑1 (MQ=255)

                                                    agaCCGTGGTCCTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCg                                                                                           <  1:5972005/150‑1 (MQ=255)

                                                    agaCCGTGGTCCTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCg                                                                                           <  1:10553967/150‑1 (MQ=255)

                                                      aCCGTGGTCCTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGc                                                                                         >  1:8329775/1‑150 (MQ=255)

                                                      aCCGTGGTCCTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGc                                                                                         <  1:8961529/150‑1 (MQ=255)

                                                       ccGTGGTCCTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGcc                                                                                        <  2:4206426/150‑2 (MQ=255)

                                                        cGTGGTCCTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGcct                                                                                       <  1:6546428/150‑3 (MQ=255)

                                                         gTGGTCCTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGgcggcggcg                                                                                                                 <  2:8401936/123‑1 (MQ=255)

                                                         gTGGTCCTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCCAGGTCGCCGCCCAACTCGGCCTg                                                                                      >  2:5883406/1‑150 (MQ=255)

                                                          tGGTCCTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTgg                                                                                     >  1:7104651/1‑150 (MQ=255)

                                                          tGGTCCTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTgg                                                                                     <  1:8418987/150‑1 (MQ=255)

                                                           ggTCCTGAACATGGCCCGGCTGGGCCTGTGCTGGCCGGATGTGGCCGAGGGCACCGGTCTCGCGCTGACCGTCGTCGCCGCCGGCCTGCTCGGCCAGCAGGCCCTGCCAGAGGCGGCGGCGCGCCAGGTCGCGGCGGACCTCgggcgggg                                                                                    >  2:6482905/1‑144 (MQ=255)

                                                           ggTCCTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGga                                                                                    <  1:1357112/150‑1 (MQ=255)

                                                            gTCCTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGgac                                                                                   <  2:2073793/150‑1 (MQ=255)

                                                             tCCTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGgacg                                                                                  <  1:8196203/150‑1 (MQ=255)

                                                                 gAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGgacgacga                                                                              >  2:9766134/1‑150 (MQ=255)

                                                                  aaCAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGgacgacgac                                                                             <  2:10069547/150‑1 (MQ=255)

                                                                      aGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCgg                                                                         >  1:3584139/1‑150 (MQ=255)

                                                                       ggCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCCGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCCACTCGGCCTGGACGACGACGCGGt                                                                        >  2:1566679/1‑150 (MQ=255)

                                                                             ccTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCct                                                                  <  1:6930334/150‑1 (MQ=255)

                                                                             ccTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGACGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCct                                                                  <  1:4704799/150‑1 (MQ=255)

                                                                              cTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCctg                                                                 <  2:5253250/150‑1 (MQ=255)

                                                                                gggCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCctgct                                                               <  1:10269904/150‑1 (MQ=255)

                                                                                    cTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCaa                                                           <  2:1422667/150‑1 (MQ=255)

                                                                                    cTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCaa                                                           <  1:444285/150‑1 (MQ=255)

                                                                                      gTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAg                                                         <  1:6294638/150‑1 (MQ=255)

                                                                                       tCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCACCTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGc                                                        <  2:9555316/150‑1 (MQ=255)

                                                                                       tCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGc                                                        <  1:10009480/150‑1 (MQ=255)

                                                                                        ccTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCa                                                       >  2:9071856/1‑150 (MQ=255)

                                                                                          tGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATc                                                     <  2:8213120/150‑1 (MQ=255)

                                                                                          tGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATc                                                     <  1:9087134/150‑1 (MQ=255)

                                                                                               ggATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCt                                                >  1:431918/1‑150 (MQ=255)

                                                                                               ggATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCt                                                <  1:7919508/150‑1 (MQ=255)

                                                                                               ggATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCt                                                >  1:5987124/1‑150 (MQ=255)

                                                                                               ggATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCt                                                <  1:10173479/150‑1 (MQ=255)

                                                                                                    tGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGcgcg                                           <  2:4046664/150‑1 (MQ=255)

                                                                                                    tGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGcgcg                                           >  2:9370269/1‑150 (MQ=255)

                                                                                                     ggCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCgg                                          <  2:3144175/150‑1 (MQ=255)

                                                                                                      gCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGgc                                         >  1:2148011/1‑150 (MQ=255)

                                                                                                       ccGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGgca                                        <  1:4213486/150‑1 (MQ=255)

                                                                                                        cGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGgcag                                       >  1:2569309/1‑150 (MQ=255)

                                                                                                         gACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGgcagc                                      <  2:8586453/150‑1 (MQ=255)

                                                                                                          aCGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGgcagca                                     >  1:2770499/1‑150 (MQ=255)

                                                                                                            ggCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCAtt                                   <  1:3751422/150‑1 (MQ=255)

                                                                                                            ggCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCAtt                                   >  1:1648249/1‑150 (MQ=255)

                                                                                                             gCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTc                                  >  1:9342639/1‑150 (MQ=255)

                                                                                                              cACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTcc                                 <  2:4722995/150‑1 (MQ=255)

                                                                                                               aCCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTccc                                <  2:6052255/150‑1 (MQ=255)

                                                                                                                ccGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCcg                               <  2:9186924/150‑1 (MQ=255)

                                                                                                                  ggTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCcggc                             <  1:6409590/150‑1 (MQ=255)

                                                                                                                  ggTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCcggc                             >  1:320830/1‑150 (MQ=255)

                                                                                                                       cGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCAt                        <  2:1155787/150‑1 (MQ=255)

                                                                                                                          ccTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTcc                     >  2:5128011/1‑150 (MQ=255)

                                                                                                                          ccTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTcc                     >  2:9164059/1‑150 (MQ=255)

                                                                                                                          ccTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCAGGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTcc                     <  2:7057244/150‑1 (MQ=255)

                                                                                                                            tGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCa                   >  2:1881121/1‑150 (MQ=255)

                                                                                                                            tGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCa                   <  2:8011810/150‑1 (MQ=255)

                                                                                                                               ccttcgTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGcg                <  1:9660836/145‑1 (MQ=255)

                                                                                                                               ccttcgTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGcg                >  1:5311902/6‑150 (MQ=255)

                                                                                                                               ccttcgTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGcg                >  1:7518781/6‑150 (MQ=255)

                                                                                                                                cttcgTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGcga               <  2:7410321/146‑1 (MQ=255)

                                                                                                                                  tcgTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGcgacc             <  2:2277968/148‑1 (MQ=255)

                                                                                                                                     tCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGcgacccga          <  2:8739811/150‑1 (MQ=255)

                                                                                                                                      cACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGcgacccgac         >  2:5548881/1‑150 (MQ=255)

                                                                                                                                       aCCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGcgacccgacc        <  1:3427407/150‑1 (MQ=255)

                                                                                                                                       aCCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGcgacccgacc        <  2:1668730/150‑1 (MQ=255)

                                                                                                                                           ccgccCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGCGACCCGACCATCt    <  1:6583874/150‑1 (MQ=255)

                                                                                                                                             gccCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGCGACCCGACCATCTAc  <  1:7303594/150‑1 (MQ=255)

                                                                                                                                             gccCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACGCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGCGACCCGACCATCtcc  <  1:4956830/150‑3 (MQ=255)

CATCCCATGCAACACAGCCAAGTGTCCCCCAACGCGCGCCAGCAACTGGCCGAGACCGTGGTCCTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTATCGCCCTGACATTAGTCACCGCCGCCATGTTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCATGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGCGACCCGACCATCTAC  >  NC_008463/3387737‑3388027

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`