Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 3,423,281 A→G K130K (AAA→AAG)  mexY → multidrug efflux RND transporter permease subunit MexY

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084633,423,2810AG98.9% 1508.5 / NA 451K130K (AAA→AAG) mexYmultidrug efflux RND transporter permease subunit MexY
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (216/229);  minor base T (5/0);  total (222/229)
Fisher's exact test for biased strand distribution p-value = 2.79e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 453 total aligned reads displayed.

CAGCAGCACCGGCCAGGCTTCGCTGACCCTGACCTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAAGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAGGGCGTGGGCAAGGTCCAGACCTGGGGCGC  >  NC_008463/3423134‑3423427
                                                                                                                                                   |                                                                                                                                                  
 agcagcACCGGCCAGGCTTCGCTGACCCTGACCTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCAGCCCGAGTCGGGGCGGCGCGACGGTATCTACGTGGAGAAGgcg                                                                                                                                                 >  1:10015133/1‑150 (MQ=255)
  gcagcaCCGGCCAGGCTTCGCTGACCCTGACCGTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGgcgg                                                                                                                                                <  1:9774049/150‑1 (MQ=255)
     gcaCCGGCCAGGCTTCGCTGACCCTGACCTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGGCGCGCCGGCCCGAGTCGGGGCGGCGCCGCGGGAACAACGGGGAGAAGGcggcgg                                                                                                                                             >  2:2032498/1‑150 (MQ=255)
      caCCGGCCAGGCTTCGCTGACCCTGACCTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGa                                                                                                                                            <  2:542613/150‑1 (MQ=255)
      caCCGGCCAGGCTTCGCTGACCCTGACCTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGa                                                                                                                                            <  1:9835853/150‑1 (MQ=255)
       aCCGGCCAGGCTTCGCTGACCCTGACCTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGAc                                                                                                                                           <  1:667153/150‑1 (MQ=255)
        ccGGCCAGGCTTCGCTGACCCTGACCTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACa                                                                                                                                          <  2:4479700/150‑1 (MQ=255)
            ccAGGCTTCGCTGACCCTGACCTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGGATCTACGTGGTGAAGGCGGCGGACAGCAt                                                                                                                                      >  1:4736649/1‑150 (MQ=255)
             cAGGCTTCGCTGACCCTGACCTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATc                                                                                                                                     <  2:9239090/150‑1 (MQ=255)
               ggCTTCGCTGACCCTGACCTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCa                                                                                                                                   <  1:1412733/150‑1 (MQ=255)
               ggCTTCGCTGACCCTGACCTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCa                                                                                                                                   <  2:933018/150‑1 (MQ=255)
                  atCGCTGACCCTGACCTGCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCt                                                                                                                                <  1:4838654/149‑1 (MQ=255)
                   tCGCTGACCCTGACCTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGTCAGCATCCAGCTg                                                                                                                               >  1:3027314/1‑150 (MQ=255)
                     gCTGACCCTGACCTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGAt                                                                                                                             <  2:1153825/150‑1 (MQ=255)
                     gCTGACCCTGACCTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGAt                                                                                                                             <  1:2865131/150‑1 (MQ=255)
                     gCTGACCCTGACCTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGAt                                                                                                                             <  1:10449985/150‑1 (MQ=255)
                      ctgaccctgaccTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGGCGGTATCTACGTGGGGAAGGCGGCGGACAGCATCCAGCTGTTc                                                                                                                            >  1:8869319/1‑150 (MQ=255)
                       tgaccctgaccTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCg                                                                                                                           <  1:4633855/150‑1 (MQ=255)
                          ccctgaccTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCa                                                                                                                        <  2:2992950/150‑1 (MQ=255)
                          ccctgaccTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCa                                                                                                                        >  1:7542649/1‑150 (MQ=255)
                           cctgaccTGCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCAc                                                                                                                       <  1:5802050/150‑1 (MQ=255)
                            ctgaccTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGGGGAGAAGGCGGCGGACCGCATCCAGCTGATCGTCAcc                                                                                                                      >  2:8665403/1‑150 (MQ=255)
                              gaccTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCt                                                                                                                    <  2:9527630/150‑1 (MQ=255)
                              gaccTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCt                                                                                                                    <  1:5130144/150‑1 (MQ=255)
                              gaccTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCGGCCCGAGTCGGTGCGGCGCGACGGGATCTACGGGGAGAAGGCGGCGGACAGCAAACCGCAGATCGTCccccg                                                                                                                    >  1:3696510/1‑149 (MQ=255)
                                ccTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCa                                                                                                                  <  1:9683295/150‑1 (MQ=255)
                                ccTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCa                                                                                                                  <  1:9440198/150‑1 (MQ=255)
                                   tCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCAcct                                                                                                               <  2:8073233/150‑1 (MQ=255)
                                        cAGGGCGTGAACGCCGACCTCGCCGCGGTGGACGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGGGAAGGCGGCGGTCAGCATGCAGCTGGTCGTCACCCTCACCtcctcc                                                                                                          >  2:3972415/1‑150 (MQ=255)
                                        cAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCtcctcc                                                                                                          <  1:592470/150‑1 (MQ=255)
                                         aGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCa                                                                                                         <  1:5646119/150‑1 (MQ=255)
                                           ggCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGc                                                                                                       <  1:3869844/150‑1 (MQ=255)
                                             cGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCgg                                                                                                     <  2:878234/150‑1 (MQ=255)
                                              gTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGc                                                                                                    <  2:7246284/150‑1 (MQ=255)
                                                 aaCGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCCTCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGc                                                                                                 >  1:7883393/1‑150 (MQ=255)
                                                    gCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTac                                                                                              <  2:4902649/150‑1 (MQ=255)
                                                    gCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTac                                                                                              <  2:1525411/150‑1 (MQ=255)
                                                       gACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTacgac                                                                                           <  1:1880692/150‑1 (MQ=255)
                                                             gccgcGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCGACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTACTCCAGCGGCCGCTACGACGCCATg                                                                                     >  1:2575004/1‑150 (MQ=255)
                                                              ccgcGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGGGGCGCGACGGTATCTACGGGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCAAgg                                                                                    >  2:4019485/1‑150 (MQ=255)
                                                              ccgcGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATgg                                                                                    <  1:2489938/150‑1 (MQ=255)
                                                                  ggTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGCGAAGGCGGCGGACCGCATCCAGCTGTTCGGCCCCCTCACCGCCGCCCGCGGCCGCTACGACGCCATGGAACt                                                                                >  2:10110190/1‑150 (MQ=255)
                                                                  ggTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACt                                                                                <  1:4463971/150‑1 (MQ=255)
                                                                    tGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTgg                                                                              <  2:6947657/150‑1 (MQ=255)
                                                                    tGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTgg                                                                              <  1:7379450/150‑1 (MQ=255)
                                                                       aaGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCg                                                                           <  1:6966542/150‑1 (MQ=255)
                                                                        aGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCga                                                                          <  2:5649156/150‑1 (MQ=255)
                                                                             aGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCg                                                                     <  2:773758/150‑1 (MQ=255)
                                                                              gAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCGACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGc                                                                    >  2:3466846/1‑150 (MQ=255)
                                                                               aaCCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGGCGGTGCGGCGCGACGGGATCTACGGGGAGAAGGCGGCGGACAGCAACCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGcc                                                                   >  2:644376/1‑150 (MQ=255)
                                                                                 ccGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTc                                                                 <  1:4021825/150‑1 (MQ=255)
                                                                                       gAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATACAGCTGATCGTCACCCTCACCACCACCCGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGGCCaa                                                           >  2:4805656/1‑150 (MQ=255)
                                                                                        aaGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAAc                                                          <  1:3186915/150‑1 (MQ=255)
                                                                                        aaGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAAc                                                          <  2:4983556/150‑1 (MQ=255)
                                                                                              gtcgAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACCGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCAAGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTg                                                    >  2:10172944/1‑150 (MQ=255)
                                                                                               tcgAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGc                                                   <  2:3389854/150‑1 (MQ=255)
                                                                                                cgAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCa                                                  <  1:7944052/150‑1 (MQ=255)
                                                                                                 gAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCCGCGGCCGCTACGACGCCCTGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAg                                                 >  2:5719959/1‑150 (MQ=255)
                                                                                                  aGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGACCTGGGCGAGATCGCCTCGTCCAACGTGTTGCAgg                                                >  2:5882093/1‑150 (MQ=255)
                                                                                                  aGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAgg                                                <  1:6561854/150‑1 (MQ=255)
                                                                                                   gTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGgc                                               >  2:528456/1‑150 (MQ=255)
                                                                                                   gTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGgc                                               >  2:9704928/1‑150 (MQ=255)
                                                                                                   gTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGgc                                               >  1:7688028/1‑150 (MQ=255)
                                                                                                    tCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGgcg                                              >  2:6618875/1‑150 (MQ=255)
                                                                                                      gcgcCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCt                                            <  2:1643540/150‑1 (MQ=255)
                                                                                                       cgcCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTg                                           <  2:3582381/150‑1 (MQ=255)
                                                                                                       cgcCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTg                                           <  1:10645162/150‑1 (MQ=255)
                                                                                                       cgcCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTg                                           >  1:2286580/1‑150 (MQ=255)
                                                                                                       cgcCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTg                                           >  2:307991/1‑150 (MQ=255)
                                                                                                       cgcCTGCCCGAGGCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCCCCTCCCCCAGCGGCCGCGACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTg                                           <  2:7539491/150‑1 (MQ=255)
                                                                                                        gcCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTgc                                          <  2:5502664/150‑1 (MQ=255)
                                                                                                          cTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTACAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTgcgc                                        <  2:9024085/150‑1 (MQ=255)
                                                                                                            gCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCg                                      >  2:2822893/1‑150 (MQ=255)
                                                                                                              ccGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCggg                                    <  2:2465167/150‑1 (MQ=255)
                                                                                                              ccGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCggg                                    <  2:10644161/150‑1 (MQ=255)
                                                                                                              ccGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACGGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCggg                                    <  2:6501896/150‑1 (MQ=255)
                                                                                                                gAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTg                                  >  2:748257/1‑150 (MQ=255)
                                                                                                                 aGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTgg                                 >  2:2376940/1‑150 (MQ=255)
                                                                                                                 aGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTgg                                 <  2:2724834/150‑1 (MQ=255)
                                                                                                                      gTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAGGGc                            >  2:6329387/1‑150 (MQ=255)
                                                                                                                       tGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAGGGCg                           <  1:848394/150‑1 (MQ=255)
                                                                                                                        gcggcgCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAGGGCGt                          >  2:3778129/1‑150 (MQ=255)
                                                                                                                        gcggcgCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAGGGCGt                          >  2:8815521/1‑150 (MQ=255)
                                                                                                                          ggcgCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAGGGCGTgg                        >  2:8307830/1‑150 (MQ=255)
                                                                                                                           gcgcGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAGGGCGTggg                       >  2:5312399/1‑150 (MQ=255)
                                                                                                                            cgcgACGGTATCTACGTGGAGACGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAGGGCGTGGGc                      <  2:2221792/150‑1 (MQ=255)
                                                                                                                             gcgACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAGGGCGTGGGCa                     <  2:5106759/150‑1 (MQ=255)
                                                                                                                             gcgACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAGGGCGTGGGCa                     <  2:4378599/150‑1 (MQ=255)
                                                                                                                                aCGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAGGGCGTGGGCAAgg                  <  1:9233393/150‑1 (MQ=255)
                                                                                                                                 cGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAGGGCGTGGGCAAGGt                 >  2:1335724/1‑150 (MQ=255)
                                                                                                                                 cGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAGGGCGTGGGCAAGGt                 >  1:6353803/1‑150 (MQ=255)
                                                                                                                                   gTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAGGGCGTGGGCAAGGTcg               >  1:8363745/1‑149 (MQ=255)
                                                                                                                                    tATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAGGGCGTGGGCAAGGTcga              >  1:6087905/1‑148 (MQ=255)
                                                                                                                                           gTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAGGGCGTGGGCAAGGTCGAGACCTgg       <  2:9588871/150‑1 (MQ=255)
                                                                                                                                            tGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAGGGCGTGGGCAAGGTCGAGACCTggg      <  2:608926/150‑1 (MQ=255)
                                                                                                                                             ggAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAGGGCGTGGGCAAGGTCGAGACCTgggg     <  1:1602562/150‑1 (MQ=255)
                                                                                                                                              gagaAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAGGGCGTGGGCAAGGTCGAGACCTGGGgc    >  1:3604872/1‑150 (MQ=255)
                                                                                                                                               agaAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAGGGCGTGGGCAAGGTCGAGACCTGGGgcg   >  1:431317/1‑150 (MQ=255)
                                                                                                                                                gaAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGACCTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAGGGCGTGGGCAAGGTCGAGACCTGGGgcgc  >  2:4700439/1‑150 (MQ=255)
                                                                                                                                                gaAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAGGGCGTGGGCAAGGTCGAGACCTGGGgcgc  <  1:5444984/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CAGCAGCACCGGCCAGGCTTCGCTGACCCTGACCTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAAGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAGGGCGTGGGCAAGGTCCAGACCTGGGGCGC  >  NC_008463/3423134‑3423427

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑