Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 3,424,152 C→G Q421E (CAA→GAG)  mexY → multidrug efflux RND transporter permease subunit MexY

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084633,424,1520CG100.0% 1954.0 / NA 491Q421E (CAA→GAG) mexYmultidrug efflux RND transporter permease subunit MexY
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (234/257);  total (234/257)

Only 100 of 491 total aligned reads displayed.

GTGGCCCTGCTCGGCACCTTCACGGTGATGCTCGGCCTGGGCTTCTCGATCAACGTGCTGACCATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCCAAGAAGGCCTGTCGCCGCACGACACCACGGTCAAGGCGATGCGCCAAATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCAACATCTACCGCCAGTT  >  NC_008463/3424008‑3424297
                                                                                                                                                |                                                                                                                                                 
 tGGCCCTGCTCGGCACCTTCACGGTGATGCTCGGCCTGGGCTTCTCGATCAACGTGCTGACCATGTTCGGCAGGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGaggaag                                                                                                                                             >  2:877404/1‑150 (MQ=255)
   gCCCTGCTCGGCACCTTCACGGTGATGCTCGGCCTGGGCTTCTCGATCAACGTGCTGACCATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGc                                                                                                                                           <  1:772877/150‑1 (MQ=255)
     ccTGCTCGGCACCTTCACGGTGATGCTCGGCCTGGGCTTCTCGATCAACGTGCTGACCATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCt                                                                                                                                         <  1:6298528/150‑1 (MQ=255)
     ccTGCTCGGCACCTTCACGGTGATGCTCGGCCTGGGCTTCTCGATCAACGTGCTGACCATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCt                                                                                                                                         <  1:995948/150‑1 (MQ=255)
       tGCTCGGCACCTTCACGGTGATGCTCGGCCTGGGCTTCTCGATCAACGTGCTGACCATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGt                                                                                                                                       >  2:399207/1‑150 (MQ=255)
       tGCTCGGCACCTTCACGGTGATGCTCGGCCTGGGCTTCTCGATCAACGTGCTGACCATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGt                                                                                                                                       <  1:3957015/150‑1 (MQ=255)
          tCGGCACCTTCACGGTGATGCTCGGCCTGGGCTTCTCGATCAACGTGCTGACCATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTcgc                                                                                                                                    >  1:1185323/1‑150 (MQ=255)
          tCGGCACCTTCACGGTGATGCTCGGCCTGGGCTTCTCGATCAACGTGCTGACCATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTcgc                                                                                                                                    >  1:10533182/1‑150 (MQ=255)
          tCGGCACCTTCACGGTGATGCTCGGCCTGGGCTTCTCGATCAACGTGCTGACCATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTcgc                                                                                                                                    <  2:7742696/150‑1 (MQ=255)
           cGGCACCTTCACGGTGATGCTCGGCCTGGGCTTCTCGATCAACGTGCTGACCATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTcgcc                                                                                                                                   >  2:10304786/1‑150 (MQ=255)
            ggCACCTTCACGGTGATGCTCGGCCTGGGCTTCTCGATCAACGTGCTGACCATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTcgccg                                                                                                                                  >  1:8761244/1‑150 (MQ=255)
               aCCTTCACGGTGATGCTCGGCCTGGGCTTCTCGATCAACGTGCTGACCATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCAc                                                                                                                               >  2:8302194/1‑150 (MQ=255)
                    cACGGTGATGCTCGGCCTGGGCTTCTCGATCAACGTGCTGACCATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGacgc                                                                                                                          >  1:8786976/1‑148 (MQ=255)
                      cGGTGATGCTCGGCCTGGGCTTCTCGATCAACGTGCTGACCATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGAcgcca                                                                                                                        >  2:5990933/1‑150 (MQ=255)
                          gATGCTCGGCCTGGGCTTCTCGATCAACGTGCTGACCATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGt                                                                                                                    <  1:564827/150‑1 (MQ=255)
                           aTGCTCGGCCTGGGCTTCTCGATCAACGTGCTGACCATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTc                                                                                                                   >  2:1046044/1‑150 (MQ=255)
                            tGCTCGGCCTGGGCTTCTCGATCAACGTGCTGACCATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCa                                                                                                                  >  1:8433787/1‑150 (MQ=255)
                             gCTCGGCCTGGGCTTCTCGATCAACGTGCTGACCATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCaa                                                                                                                 <  1:3202237/150‑1 (MQ=255)
                             gCTCGGCCTGGGCTTCTCGATCAACGTGCTGACCATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCaa                                                                                                                 <  2:4404870/150‑1 (MQ=255)
                             gCTCGGCCTGGGCTTCTCGATCAACGTGCTGACCATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCaa                                                                                                                 <  2:4499031/150‑1 (MQ=255)
                               tCGGCCTGGGCTTCTCGATCAACGTGCTGACCATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAgg                                                                                                               <  2:2301993/150‑1 (MQ=255)
                                    cTGGGCTTCTCGATCAACGTGCTGACCATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATg                                                                                                          >  1:8082373/1‑150 (MQ=255)
                                     tGGGCTTCTCGATCAACGTGCTGACCATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTCGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATgc                                                                                                         >  2:4284471/1‑150 (MQ=255)
                                      gggCTTCTCGATCAACGTGCTGACCATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATgcg                                                                                                        >  2:266329/1‑150 (MQ=255)
                                         cTTCTCGATCAACGTGCTGACCATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGTGGAAGGCCTGTCGCCGCTCGACGCCACGGTCAAGGCGATGCGCCa                                                                                                     <  2:5644584/150‑1 (MQ=21)
                                               gATCAACGTGCTGACCATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAg                                                                                               >  1:7126069/1‑150 (MQ=255)
                                                aTCAACGTGCTGACCATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGc                                                                                              <  2:1829636/150‑1 (MQ=255)
                                                aTCAACGTGCTGACCATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGc                                                                                              >  2:8524727/1‑150 (MQ=255)
                                                aTCAACGTGCTGACCATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGc                                                                                              >  2:6346539/1‑150 (MQ=255)
                                                 tCAACGTGCTGACCATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCg                                                                                             >  1:4634892/1‑150 (MQ=255)
                                                    aCGTGCTGACCATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCgggg                                                                                          >  2:1543035/1‑150 (MQ=255)
                                                       tGCTGACCATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCa                                                                                       <  1:7025725/150‑1 (MQ=255)
                                                        gCTGACCATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCAt                                                                                      >  1:8548885/1‑150 (MQ=255)
                                                         cTGACCATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCAtc                                                                                     >  2:9163110/1‑150 (MQ=255)
                                                           gACCATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCAtcgt                                                                                   <  2:3321993/150‑1 (MQ=255)
                                                            aCCATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCAtcgtc                                                                                  >  1:7443343/1‑150 (MQ=255)
                                                            aCCATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCAtcgtc                                                                                  <  2:7483695/150‑1 (MQ=255)
                                                              cATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCgg                                                                                <  2:7278344/150‑1 (MQ=255)
                                                               aTGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGc                                                                               <  1:7915170/150‑1 (MQ=255)
                                                                tGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCa                                                                              >  1:1760347/1‑150 (MQ=255)
                                                                 gTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCAt                                                                             >  2:499769/1‑150 (MQ=255)
                                                                  ttCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATc                                                                            <  1:1610563/150‑1 (MQ=255)
                                                                    cGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCAc                                                                          <  1:409184/150‑1 (MQ=255)
                                                                    cGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCAc                                                                          <  1:8302194/150‑1 (MQ=255)
                                                                        aTGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCgtg                                                                      <  1:3636874/150‑1 (MQ=255)
                                                                        aTGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCgtg                                                                      >  1:8244056/1‑150 (MQ=255)
                                                                        aTGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCgtg                                                                      >  1:2115506/1‑150 (MQ=255)
                                                                           gTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCgtggtg                                                                   >  2:650745/1‑150 (MQ=255)
                                                                            tCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGc                                                                  <  1:760342/150‑1 (MQ=255)
                                                                             ccTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCt                                                                 <  2:9848853/150‑1 (MQ=255)
                                                                             ccTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCt                                                                 <  1:124466/150‑1 (MQ=255)
                                                                              cTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTg                                                                >  2:5388856/1‑150 (MQ=255)
                                                                              cTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTg                                                                >  1:3360898/1‑150 (MQ=255)
                                                                               tGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTgg                                                               >  1:424350/1‑150 (MQ=255)
                                                                                ggCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGt                                                              <  1:6435903/150‑1 (MQ=255)
                                                                                ggCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGt                                                              >  2:1068614/1‑150 (MQ=255)
                                                                                    aTCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCg                                                          >  1:672304/1‑150 (MQ=255)
                                                                                       ggCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGGCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTa                                                       >  2:6834161/1‑150 (MQ=25)
                                                                                         cATCCTGGTGGACGACGCGATCATCGGGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCCCGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTAtt                                                     <  2:9653838/150‑1 (MQ=37)
                                                                                          aTCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTc                                                    <  2:5779658/150‑1 (MQ=255)
                                                                                             cTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCACGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTg                                                 <  1:5142493/150‑1 (MQ=37)
                                                                                             cTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTg                                                 >  1:9318472/1‑150 (MQ=255)
                                                                                               ggtggACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGcc                                               >  2:693561/1‑150 (MQ=255)
                                                                                                gtggACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCg                                              <  2:509261/150‑1 (MQ=255)
                                                                                                gtggACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCg                                              >  2:2787474/1‑150 (MQ=255)
                                                                                                 tggACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGa                                             <  2:1979233/150‑1 (MQ=255)
                                                                                                  ggACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGAt                                            <  1:7330948/150‑1 (MQ=255)
                                                                                                   gacgacGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATg                                           <  2:3781448/150‑1 (MQ=255)
                                                                                                    acgacgCGATCATCGTGGTGGAGTACGTGGAGCGGCTGTTGGCCGTGGAAGGCCTGTCGCCGCACGACGCCTCGGTCAAGGCGATGCGCCAGATCTGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATgg                                          <  2:317631/150‑1 (MQ=255)
                                                                                                     cgacgCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGc                                         >  1:6074792/1‑150 (MQ=255)
                                                                                                        cgcgATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGtt                                      <  2:6151989/150‑1 (MQ=255)
                                                                                                         gcgATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGttc                                     >  1:2661254/1‑150 (MQ=255)
                                                                                                          cgATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGttct                                    >  1:1522698/1‑150 (MQ=255)
                                                                                                           gATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGttctt                                   <  1:5903151/150‑1 (MQ=255)
                                                                                                            atcatcGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTCTGCGTGCCGATGGCGttcttc                                  >  2:1621573/1‑150 (MQ=37)
                                                                                                            atcatcGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGttcttc                                  <  2:3514467/150‑1 (MQ=255)
                                                                                                             tcatcGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGttcttcc                                 >  2:4114846/1‑149 (MQ=255)
                                                                                                                tcGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAgcg                              <  2:8399371/150‑1 (MQ=255)
                                                                                                                tcGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAgcg                              <  2:1002669/150‑1 (MQ=255)
                                                                                                                tcGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAgcg                              >  1:7471439/1‑150 (MQ=255)
                                                                                                                tcGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAgcg                              >  1:9190208/1‑150 (MQ=255)
                                                                                                                tcGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAgcg                              <  1:955726/150‑1 (MQ=255)
                                                                                                                  gtggtgGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAgcggc                            <  1:9768510/150‑1 (MQ=255)
                                                                                                                  gtggtgGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAgcggc                            <  2:2218762/150‑1 (MQ=255)
                                                                                                                   tggtggAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAgcggcg                           <  1:10691545/150‑1 (MQ=255)
                                                                                                                       ggAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGt                       <  2:10190839/150‑1 (MQ=255)
                                                                                                                          gaACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGGGGTGCTGGTCTCGGTCTTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTggg                    >  2:9480146/1‑150 (MQ=38)
                                                                                                                            aCGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCa                  <  2:479599/150‑1 (MQ=255)
                                                                                                                                 gAGCGGCTGATGGCCGAGGAAGGCCTTTCGCCGAACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCAACATc             <  2:711054/150‑1 (MQ=38)
                                                                                                                                 gAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCAACATc             <  1:10079563/150‑1 (MQ=255)
                                                                                                                                 gAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCAACATc             >  1:8962642/1‑150 (MQ=255)
                                                                                                                                  aGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCAACATCt            >  1:3073710/1‑150 (MQ=255)
                                                                                                                                   gCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCAACATCTa           <  1:6597186/150‑1 (MQ=255)
                                                                                                                                    cGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCAACATCTAc          >  1:10168299/1‑150 (MQ=255)
                                                                                                                                    cGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCAACATCTAc          <  2:5231137/150‑1 (MQ=255)
                                                                                                                                     ggCTGCTGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGTCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCAACATCTAcc         <  1:717292/150‑1 (MQ=38)
                                                                                                                                     ggCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCAACATCTAcc         <  1:5434126/150‑1 (MQ=255)
                                                                                                                                          aTGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGGTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCAACATCTACCGCCAg    <  2:1197567/150‑1 (MQ=39)
                                                                                                                                          aTGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCAACATCTACCGCCAg    >  2:7177014/1‑150 (MQ=255)
                                                                                                                                           tGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCAACATCTACCGCCAGt   >  1:2391377/1‑150 (MQ=255)
                                                                                                                                                |                                                                                                                                                 
GTGGCCCTGCTCGGCACCTTCACGGTGATGCTCGGCCTGGGCTTCTCGATCAACGTGCTGACCATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCCAAGAAGGCCTGTCGCCGCACGACACCACGGTCAAGGCGATGCGCCAAATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCAACATCTACCGCCAGTT  >  NC_008463/3424008‑3424297

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑