Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 3,424,199 A→G Q436Q (CAA→CAG)  mexY → multidrug efflux RND transporter permease subunit MexY

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084633,424,1990AG100.0% 1894.4 / NA 476Q436Q (CAA→CAG) mexYmultidrug efflux RND transporter permease subunit MexY
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (234/242);  total (234/242)

Only 100 of 476 total aligned reads displayed.

CTCGATCAACGTGCTGACCATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCCAAGAAGGCCTGTCGCCGCACGACACCACGGTCAAGGCGATGCGCCAAATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCAACATCTACCGCCAGTTCGCGGTGACCCTGGCGGTCTCCATCGGCTTCTCGGCGTTCCTCGCGCT  >  NC_008463/3424052‑3424345
                                                                                                                                                   |                                                                                                                                                  
 tcGATCAACGTGCTGACCATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATc                                                                                                                                                 >  1:3172097/1‑150 (MQ=255)
 tcGATCAACGTGCTGACCATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATc                                                                                                                                                 >  1:7166825/1‑150 (MQ=255)
  cGATCAACGTGCTGACCATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCa                                                                                                                                                <  1:3330127/150‑1 (MQ=255)
     acAACGTGCTGACCATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCg                                                                                                                                             <  1:1188064/149‑1 (MQ=255)
      cAACGTGCTGACCATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCgg                                                                                                                                            <  1:4385988/150‑1 (MQ=255)
       aaCGTGCTGACCATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGGCGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCggg                                                                                                                                           >  1:5622447/1‑150 (MQ=21)
          gTGCTGACCATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGcc                                                                                                                                        <  2:10184552/150‑1 (MQ=255)
           tGCTGACCATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCa                                                                                                                                       >  1:488340/1‑150 (MQ=255)
             cTGACCATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCAtc                                                                                                                                     <  2:5380153/150‑1 (MQ=255)
               gACCATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCAtcgt                                                                                                                                   <  1:7200004/150‑1 (MQ=255)
               gACCATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCAtcgt                                                                                                                                   <  2:711167/150‑1 (MQ=255)
                aCCATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCAtcgtc                                                                                                                                  <  2:7483695/150‑1 (MQ=255)
                 ccATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCAtcgtcg                                                                                                                                 <  2:2453739/150‑1 (MQ=255)
                 ccATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCAtcgtcg                                                                                                                                 <  1:8126885/150‑1 (MQ=255)
                 ccATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCAtcgtcg                                                                                                                                 <  1:10308296/150‑1 (MQ=255)
                 ccAGGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCAtcgtcg                                                                                                                                 <  2:5053824/150‑1 (MQ=21)
                  cATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCgg                                                                                                                                <  2:4072316/150‑1 (MQ=255)
                  cATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCgg                                                                                                                                <  2:7278375/150‑1 (MQ=255)
                   aTGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGc                                                                                                                               <  1:8687353/150‑1 (MQ=255)
                    tGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCa                                                                                                                              >  2:5941311/1‑150 (MQ=255)
                     gtacGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCAACGTCGGCAt                                                                                                                             >  2:10362347/4‑150 (MQ=21)
                     gTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGctt                                                                                                                             <  2:5760274/150‑3 (MQ=255)
                       tCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCa                                                                                                                           <  1:2146130/150‑1 (MQ=255)
                        cGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCAc                                                                                                                          <  1:8302194/150‑1 (MQ=255)
                        cGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCAc                                                                                                                          <  2:7733029/150‑1 (MQ=255)
                         ggCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCAcc                                                                                                                         <  1:1084138/150‑1 (MQ=255)
                            aTGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCgtg                                                                                                                      <  1:3636874/150‑1 (MQ=255)
                            aTGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCgtg                                                                                                                      >  1:9949368/1‑150 (MQ=255)
                             tGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCgtgg                                                                                                                     >  1:10681914/1‑150 (MQ=255)
                              ggTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCgtggt                                                                                                                    >  2:6172319/1‑150 (MQ=255)
                                tCCTGGCGCGCGGCATCCTGGTGGACGCCGCGCTCATCGTGGGGGAGAACGTCGAGCGGCAGATGGCCGAGGAAGGCCTGCCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGAACAGCGGGGCCATCGTCGGCATCACCGTGGTGc                                                                                                                  <  2:7220206/150‑1 (MQ=255)
                                tCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGCGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGc                                                                                                                  <  1:2438412/150‑1 (MQ=25)
                                tCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGc                                                                                                                  <  1:760342/150‑1 (MQ=255)
                                 ccTGGCGATCGGCATCCTGGTGGACGACGCGAACATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCt                                                                                                                 >  1:4211585/1‑150 (MQ=25)
                                    ggCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGt                                                                                                              <  2:6948588/150‑1 (MQ=255)
                                       gATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGtctc                                                                                                           <  2:4709943/150‑1 (MQ=255)
                                        aTCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCg                                                                                                          >  1:4061399/1‑150 (MQ=255)
                                           ggCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTa                                                                                                       >  1:5165658/1‑150 (MQ=255)
                                           ggCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGGCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTa                                                                                                       >  2:6834161/1‑150 (MQ=25)
                                            gCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTAt                                                                                                      <  2:1200142/150‑1 (MQ=255)
                                                 cTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTg                                                                                                 <  2:1889629/150‑1 (MQ=255)
                                                 cTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTg                                                                                                 >  1:9318472/1‑150 (MQ=255)
                                                   ggtggACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCTCACGACTCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATAGTCGGCATCAACGTGGTGCTGGTCTCGGTATTCGTGcc                                                                                               <  2:1720298/150‑1 (MQ=37)
                                                   ggtggACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGcc                                                                                               >  1:6184051/1‑150 (MQ=255)
                                                      ggACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGAt                                                                                            <  1:7044269/150‑1 (MQ=255)
                                                      ggACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGAt                                                                                            <  1:6511800/150‑1 (MQ=255)
                                                      ggACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGAt                                                                                            <  1:8455114/150‑1 (MQ=255)
                                                        acgacgCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATgg                                                                                          <  1:1784583/150‑1 (MQ=255)
                                                         cgacgCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGc                                                                                         >  1:6074792/1‑150 (MQ=255)
                                                         cgacgCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGc                                                                                         >  2:2272973/1‑150 (MQ=255)
                                                             gcgATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGttc                                                                                     <  1:1270128/150‑1 (MQ=255)
                                                             gcgATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGttc                                                                                     >  1:2661254/1‑150 (MQ=255)
                                                              cgATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGttct                                                                                    >  1:1522705/1‑150 (MQ=255)
                                                                atcatcGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTCTGCGTGCCGATGGCGttcttc                                                                                  >  2:1621573/1‑150 (MQ=37)
                                                                atcatcGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGttcttc                                                                                  <  2:6438587/150‑1 (MQ=255)
                                                                  catcGTGGTGGAGTACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAg                                                                                <  2:4398057/150‑1 (MQ=38)
                                                                  catcGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAg                                                                                <  2:8483143/150‑1 (MQ=255)
                                                                    tcGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTCTTCGTGCCGATGGCGTTCTTCAgcg                                                                              >  1:7471369/1‑150 (MQ=38)
                                                                    tcGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAgcg                                                                              <  2:1002669/150‑1 (MQ=255)
                                                                    tcGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAgcg                                                                              <  1:955726/150‑1 (MQ=255)
                                                                    tcGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAgcg                                                                              >  1:9190208/1‑150 (MQ=255)
                                                                         gtggAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGcgcg                                                                         <  2:2043730/150‑1 (MQ=255)
                                                                             agaACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTTTTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTgg                                                                     <  2:3248344/150‑1 (MQ=38)
                                                                             agaACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTgg                                                                     <  2:9604841/150‑1 (MQ=255)
                                                                                    cGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCAACAt                                                              <  2:5129271/150‑1 (MQ=255)
                                                                                     gAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCAACATc                                                             >  1:8962633/1‑150 (MQ=255)
                                                                                     gAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCAACATc                                                             <  1:10079563/150‑1 (MQ=255)
                                                                                     gAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCAACATc                                                             <  1:2813258/150‑1 (MQ=255)
                                                                                      aGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCAACATCt                                                            >  1:6745611/1‑150 (MQ=255)
                                                                                        cGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCAACATCTAc                                                          >  1:1709781/1‑150 (MQ=255)
                                                                                        cGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCAACATCTAc                                                          >  1:10168299/1‑150 (MQ=255)
                                                                                          gCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCAACATCTACCg                                                        >  2:9773390/1‑150 (MQ=255)
                                                                                           cTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCAACATCTACCGc                                                       >  2:2750625/1‑150 (MQ=255)
                                                                                                   cgaggaagGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCAACATCTACCGCCAGTTcgc                                               <  2:3731405/146‑1 (MQ=255)
                                                                                                   cgaggaagGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCAACATCTACCGCCAGTTcgc                                               >  2:6906255/5‑150 (MQ=255)
                                                                                                   cgaggaagGCATGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCAACATCTACCGCCAGTTcgc                                               >  2:8994421/5‑150 (MQ=255)
                                                                                                    gaggaagGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCAACATCTACCGCCAGTTcgcg                                              <  1:4685731/147‑1 (MQ=255)
                                                                                                     aggaagGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCAACATCTACCGCCAGTTCGCgg                                             <  2:86109/148‑1 (MQ=255)
                                                                                                      ggaagGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCAACATCTACCGCCAGTTCGCGGt                                            >  2:3820408/2‑150 (MQ=255)
                                                                                                            ccTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCAACATCTACCGCCAGTTCGCGGTGACCCt                                      <  2:1474016/150‑1 (MQ=255)
                                                                                                            ccTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCAACATCTACCGCCAGTTCGCGGTGACCCt                                      >  1:5839323/1‑150 (MQ=255)
                                                                                                              tGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCAACATCTACCGCCAGTTCGCGGTGACCCTgg                                    >  1:7715575/1‑150 (MQ=255)
                                                                                                               gTCGCCTCACGACGCCCCTGTCTAGGCGATGCGCCAGAACAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTGGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCAACAGCTACCGCCAGTTCGCGGTGACCCTGGc                                   <  2:2788761/150‑1 (MQ=255)
                                                                                                                   ccgcACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCAACATCTACCGCCAGTTCGCGGTGACCCTGGCGGtc                               <  1:3508235/150‑1 (MQ=255)
                                                                                                                      cACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCAACATCTACCGCCAGTTCGCGGTGACCCTGGCGGTCTcc                            >  2:1093872/1‑150 (MQ=255)
                                                                                                                       acgacgCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCAACATCTACCGCCAGTTCGCGGTGACCCTGGCGGTCTCCa                           <  2:8078869/150‑1 (MQ=255)
                                                                                                                       acgacgCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCAACATCTACCGCCAGTTCGCGGTGACCCTGGCGGTCTCCa                           <  2:828748/150‑1 (MQ=255)
                                                                                                                       acgacgCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCGGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCAACATCTACCGCCAGTTCGCGGTGACCCTGGCGGTCTCCa                           <  2:4120251/150‑1 (MQ=255)
                                                                                                                            gccacGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCAACATCTACCGCCAGTTCGCGGTGACCCTGGCGGTCTCCATCGGc                      >  1:7961281/2‑150 (MQ=255)
                                                                                                                             ccacGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCAACATCTACCGCCAGTTCGCGGTGACCCTGGCGGTCTCCATCGGCt                     >  2:7894412/1‑150 (MQ=255)
                                                                                                                             ccacGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCAACATCTACCGCCAGTTCGCGGTGACCCTGGCGGTCTCCATCGGCt                     >  1:3855124/1‑150 (MQ=255)
                                                                                                                               acGGTCACGGCGCTGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCGGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCAACATCTACCGCCAGATCGCGGTGACCCTGGCGGTCTCCATCGGCTtc                   <  2:9389075/150‑1 (MQ=255)
                                                                                                                                   tCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCAACATCTACCGCCAGTTCGCGGTGACCCTGGCGGTCTCCATCGGCTTCTCgg               >  1:6423553/1‑150 (MQ=255)
                                                                                                                                       ggCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCAACATCTACCGCCAGTTCGCGGTGACCCTGGCGGTCTCCATCGGCTTCTCGGCGtt           >  1:976323/1‑150 (MQ=255)
                                                                                                                                       ggCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCAACATCTACCGCCAGTTCGCGGTGACCCTGGCGGTCTCCATCGGCTTCTCGGCGtt           >  2:3337535/1‑150 (MQ=255)
                                                                                                                                       ggCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCAACATCTACCGCCAGTTCGCGGTGACCCTGGCGGTCTCCATCGGCTTCTCGGCGtt           >  2:4832213/1‑150 (MQ=255)
                                                                                                                                         cGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCAACATCTACCGCCAGTTCGCGGTGACCCTGGCGGTCTCCATCGGCTTCTCGGCGTTcc         >  2:6695187/1‑150 (MQ=255)
                                                                                                                                          gATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCAACATCTACCGCCAGTTCGCGGTGACCCTGGCGGTCTCCATCGGCTTCTCGGCGTTCCt        <  1:8815535/150‑1 (MQ=255)
                                                                                                                                          gATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCAACATCTACCGCCAGTTCGCGGTGACCCTGGCGGTCTCCATCGGCTTCTCGGCGTTCCt        >  1:7367382/1‑150 (MQ=255)
                                                                                                                                               gcCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCAACATCTACCGCCAGTTCGCGGTGACCCTGGCGGTCTCCATCGGCTTCTCGGCGTTCCTCgcgc   <  1:8250271/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CTCGATCAACGTGCTGACCATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCCAAGAAGGCCTGTCGCCGCACGACACCACGGTCAAGGCGATGCGCCAAATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCAACATCTACCGCCAGTTCGCGGTGACCCTGGCGGTCTCCATCGGCTTCTCGGCGTTCCTCGCGCT  >  NC_008463/3424052‑3424345

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑