Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 3,566,716 A→G pseudogene (1067/1275 nt) PA14_RS16215 → fatty acid desaturase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084633,566,7160AG100.0% 1859.0 / NA 470pseudogene (1067/1275 nt)PA14_RS16215fatty acid desaturase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (220/250);  total (220/250)

Only 100 of 474 total aligned reads displayed.

GCGTTCGTTCGCTGGAGCCTGGGCGTGCTGCTGATCGACATCCCGCTGCGGGTGTTCTCCTTCATGCAGGACCTGCCCAGCCACGACTTCCACCACCCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACAAGGCGCGCCCCTCAAAGTTCGGCCCGATGACCAAAACCTGGGGCATGTGGGAAAACTTCCTGATCCTGCGCAACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGG‑ATCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCT  >  NC_008463/3566568‑3566861
                                                                                                                                                    |                                                                                                                                                  
 cgttcgttCTCTGGAGCCTGGGCGTGCTGCTGATCGACATCCCGCTGCGGGTGTTCTCCTTCATGCAGGACCTGCCCAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAgg                                                                                                                                                 <  2:6976680/150‑1 (MQ=255)
 cgttcgttCGCTGGAGCCTGGGCGTGCTGCTGATCGACATCCCGCTGCGGGTGTTCTCCTTCATGCAGGACCTGCCCAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAgg                                                                                                                                                 <  1:2472999/150‑1 (MQ=255)
 cgttcgttCGCTGGAGCCTGGGCGTGCTGCTGATCGACATCCCGCTGCGGGTGTTCTCCTTCATGCAGGACCTGCCCAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAgg                                                                                                                                                 <  2:4507524/150‑1 (MQ=255)
  gttcgttcGCTGGAGCCTGGGCGTGCTGCTGATCGACATCCCGCTGCGGGTGTTCTCCTTCATGCAGGACCTGCCCAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGgc                                                                                                                                                <  1:8264646/150‑1 (MQ=255)
   ttcgttcgCTGGAGCCTGGGCGTGCTGCTGATCGACATCCCGCTGCGGGTGTTCTCCTTCATGCAGGACCTGCCCAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGgcg                                                                                                                                               >  2:2299435/1‑150 (MQ=255)
         cgCTGGAGCCTGGGCGTGCTGCTGATCGACATCCCGCTGCGGGTGTTCTCCTTCATGCAGGACCTGCCCAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGcccc                                                                                                                                         <  1:8770531/150‑1 (MQ=255)
         cgCTGGAGCCTGGGCGTGCTGCTGATCGACATCCCGCTGCGGGTGTTCTCCTTCATGCAGGACCTGCCCAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGcccc                                                                                                                                         <  2:1078525/150‑1 (MQ=255)
           cTGGAGCCTGGGCGTGCTGCTGATCGACATCCCGCTGCGGGTGTTCTCCTTCATGCAGGACCTGCCCAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTc                                                                                                                                       <  2:1309350/150‑1 (MQ=255)
                gCCTGGGCGTGCTGCTGATCGACATCCCGCTGCGGGTGTTCTCCTTCATGCAGGACCTGCCCAGCCACGACTTTCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGt                                                                                                                                  <  1:8180254/150‑1 (MQ=255)
                gCCTGGGCGTGCTGCTGATCGACATCCCGCTGCGGGTGTTCTCCTTCATGCAGGACCTGCCCAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGt                                                                                                                                  <  2:3584350/150‑1 (MQ=255)
                  cTGGGCGTGCTGCTGATCGACATCCCGCTGCGGGTGTTCTCCTTCATGCAGGACCTGCCCAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTc                                                                                                                                <  2:2774824/150‑1 (MQ=255)
                   tGGGCGTGCTGCTGATCGACATCCCGCTGCGGGTGTTCTCCTTCATGCAGGACCTGCCCAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCg                                                                                                                               >  1:8903752/1‑150 (MQ=255)
                    gggCGTGCTGCTGATCGACATCCCGCTGCGGGTGTTCTCCTTCATGCAGGACCTGCCCAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCgg                                                                                                                              >  1:2577056/1‑150 (MQ=255)
                       cGTGCTGCTGATCGACATCCCGCTGCGGGTGTTCTCCTTCATGCAGGACCTGCCCAGCCACGACGTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGccc                                                                                                                           <  2:7931440/150‑1 (MQ=255)
                          gctgctGATCGACATCCCGCTGCGGGTGTTCTCCTTCATGCAGGACCTGCCCAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGAt                                                                                                                        <  1:10059928/150‑1 (MQ=255)
                          gctgctGATCGACATCCCGCTGCGGGTGTTCTCCTTCATGCAGGACCTGCCCAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGAt                                                                                                                        <  1:5217406/150‑1 (MQ=255)
                           ctgctgATCGACATCCCGCTGCGGGTGTTCTCCTTCATGCAGGACCTGCCCAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATg                                                                                                                       <  2:2640026/150‑1 (MQ=255)
                             gctgATCGACATCCCGCTGCGGGTGTTCTCCTTCATGCAGGACCTGCCCAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGAc                                                                                                                     <  1:4828663/150‑1 (MQ=255)
                              ctgATCGACATCCCGCTGCGGGTGTTCTCCTTCATGCAGGACCTGCCCAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGAcc                                                                                                                    >  2:409633/1‑150 (MQ=255)
                              ctgATCGACATCCCGCTGCGGGTGTTCTCCTTCATGCAGGACCTGCCCAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGAcc                                                                                                                    >  2:4667017/1‑150 (MQ=255)
                                 aTCGACATCCCGCTGCGGGTGTTCTCCTTCATGCAGGACCTGCCCAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGAccgag                                                                                                                 <  2:7104553/150‑4 (MQ=255)
                                  tCGACATCCCGCTGCGGGTGTTCTCCTTCATGCAGGACCTGCCCAGCCACGACTTCCGCCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGAccgaga                                                                                                                >  1:10406590/1‑146 (MQ=255)
                                  tCGACATCCCGCTGCGGGTGTTCTCCTTCATGCAGGACCTGCCCAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGAccgaga                                                                                                                <  1:4338845/150‑5 (MQ=255)
                                   cGACATCCCGCTGCGGGTGTTCTCCTTCATGCAGGACCTGCCCAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGAccgagac                                                                                                               <  2:2141550/150‑6 (MQ=255)
                                    gACATCCCGCTGCGGGTGTTCTCCTTCATGCAGGACCTGCCCAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGAcc                                                                                                              <  1:1751195/150‑1 (MQ=255)
                                        tCCCGCTGCGGGTGTTCTCCTTCATGCAGGACCTGCCCAGCCACGACTTCCACCA‑CCGCCAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTggg                                                                                                          <  2:5685928/150‑1 (MQ=255)
                                        tCCCGCTGCGGGTGTTCTCCCTCCAGCAGGACCTGCCCAGCCACGACTTCCACC‑CCCGCACGCCGGGGGAGAACTACTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGACACCTggg                                                                                                          <  2:7460287/150‑1 (MQ=255)
                                             cTGCGGGTGTTCTCCTTCATGCAGGACCTGCCCAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCAtg                                                                                                     >  2:1541145/1‑150 (MQ=255)
                                              tGCGGGTGTTCTCCTTCATGCAGGACCTGCCCAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCAtgt                                                                                                    >  2:4007446/1‑150 (MQ=255)
                                                cGGGTGTTCTCCTTCATGCAGGACCTGCCCAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTgg                                                                                                  <  1:8142841/150‑1 (MQ=255)
                                                cGGGTGTTCTCCTTCATGCAGGACCTGCCCAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTgg                                                                                                  >  1:7715942/1‑150 (MQ=255)
                                                   gtgtTCTCCTTCATGCAGGACCTGCCCAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGag                                                                                               >  2:9505460/1‑149 (MQ=255)
                                                   gtgtTCTCCTTCATGCAGGACCTGCCCAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGag                                                                                               <  1:761301/150‑2 (MQ=255)
                                                     gtTCTCCTTCATGCAGGACCTGCCCAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGagaa                                                                                             >  2:3419406/1‑150 (MQ=255)
                                                      ttCTCCTTCATGCAGGACCTGCCCAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAAc                                                                                            >  2:5157230/1‑150 (MQ=255)
                                                       tctcCTTCATGCAGGACCTGCCCAGCCTCGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACt                                                                                           <  2:5494874/150‑1 (MQ=255)
                                                         tcCTTCATGCAGGACCTGCCCAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTc                                                                                         <  2:6478976/150‑1 (MQ=255)
                                                             tCATGCAGGACCTGACCAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGa                                                                                     <  1:4529125/150‑1 (MQ=255)
                                                              cATGCAGGACCTGCCCAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGAt                                                                                    >  2:9784312/1‑150 (MQ=255)
                                                              cATGCAGGACCAGCCCAGCCACGACTTCCACCACCC‑CACGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGGTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGAt                                                                                    <  1:6479815/150‑1 (MQ=255)
                                                                tGCAGGACCTGCCCAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATcc                                                                                  <  1:5702688/150‑1 (MQ=255)
                                                                 gCAGGACCTGCCCAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCt                                                                                 >  1:9856844/1‑150 (MQ=255)
                                                                   aGGACCTGCCCAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTgc                                                                               <  2:3928549/150‑1 (MQ=255)
                                                                   aGGACCTGCCCAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTgc                                                                               <  1:666376/150‑1 (MQ=255)
                                                                     gACCTGCCCAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTgcgc                                                                             <  1:10093976/150‑1 (MQ=255)
                                                                      aCCTGCCCAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTgcgcg                                                                            >  2:8402263/1‑149 (MQ=255)
                                                                       ccTGCCCAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTgcgcga                                                                           >  2:4846452/1‑148 (MQ=255)
                                                                       ccTGCCCAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTgcgcga                                                                           <  2:8801060/150‑3 (MQ=255)
                                                                       ccTGCCCAGCCACGACTTACACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGCCCTGGGGCATGTGGGAGAACTGCCTGATCCTgcgcga                                                                           >  2:10201354/1‑148 (MQ=255)
                                                                        cTGCCCAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGac                                                                          >  2:2500786/1‑150 (MQ=255)
                                                                           cccAGCCACGCCTCCCACC‑CCCGCACCCCGGGGGTGAACTTCCGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTAGAAGTTCGGCCCGATGACCGAGACCTGTGGCATGTGTGAGAACTTCATGATCCTGCGCGaccac                                                                       <  2:184986/150‑1 (MQ=255)
                                                                           cccAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGaccac                                                                       <  2:4750379/150‑1 (MQ=255)
                                                                            ccAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGaccacc                                                                      <  1:194633/150‑1 (MQ=255)
                                                                             cAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACct                                                                     >  2:284824/1‑150 (MQ=255)
                                                                             cAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACct                                                                     >  2:861502/1‑150 (MQ=255)
                                                                              aGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACctc                                                                    <  2:311406/150‑1 (MQ=255)
                                                                               gCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACctct                                                                   >  2:6021926/1‑150 (MQ=255)
                                                                                ccACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTg                                                                  <  1:10445649/150‑1 (MQ=255)
                                                                                  aCGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGcc                                                                >  1:6067230/1‑150 (MQ=255)
                                                                                  aCGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGcc                                                                >  1:8636305/1‑150 (MQ=255)
                                                                                     aCTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCcgcg                                                             >  1:6952781/1‑150 (MQ=255)
                                                                                     aCTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCcgcg                                                             <  2:2312571/150‑1 (MQ=255)
                                                                                      cTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCgg                                                            >  1:7151442/1‑150 (MQ=255)
                                                                                       ttCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGc                                                           >  2:7199129/1‑150 (MQ=255)
                                                                                        tCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGcc                                                          <  1:2807550/150‑1 (MQ=255)
                                                                                          cacca‑cCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAg                                                        <  2:9088026/150‑1 (MQ=255)
                                                                                          cacca‑cCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAg                                                        <  1:5052872/150‑1 (MQ=255)
                                                                                            accaccGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGt                                                       >  2:5843830/5‑150 (MQ=255)
                                                                                                ccGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGa                                                   <  1:9498563/150‑1 (MQ=255)
                                                                                                  gCAAGCCGGGGGTGAACTTCTGGCGCTTCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGAcc                                                 <  2:4627002/150‑1 (MQ=255)
                                                                                                   cAAGCCGGGGGTGAACTTCTGGCGCATCTCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGAccc                                                <  2:199587/150‑1 (MQ=255)
                                                                                                     aGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGt                                              <  1:10187048/150‑1 (MQ=255)
                                                                                                      gCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGtt                                             >  2:8510548/1‑150 (MQ=255)
                                                                                                       ccGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTc                                            <  1:9942701/150‑1 (MQ=255)
                                                                                                        cGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCg                                           <  1:7535727/150‑1 (MQ=255)
                                                                                                           gggTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGG‑aa                                       >  2:3688088/1‑149 (MQ=255)
                                                                                                               gAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGG‑aatcgt                                   <  1:5843355/150‑6 (MQ=255)
                                                                                                               gAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGG‑aatcgt                                   >  1:2217185/1‑145 (MQ=255)
                                                                                                                aaCTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAAtcgtc                                   >  2:6706099/1‑150 (MQ=255)
                                                                                                                aaCTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAAtcgtc                                   <  1:9246863/150‑1 (MQ=255)
                                                                                                                  cTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAATCGTCGa                                 >  1:1485869/1‑150 (MQ=255)
                                                                                                                  cTTCTGGCGCATCGCGCCGGAGCCCGCGGCCCACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGGCGGCCCCGTTCGGAATCGTCGa                                 >  2:10604951/1‑150 (MQ=255)
                                                                                                                        gcgcATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAATCGTCGACTGGTa                           <  2:1806154/150‑1 (MQ=255)
                                                                                                                        gcgcATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAATCGTCGACTGGTa                           <  1:2348342/150‑1 (MQ=255)
                                                                                                                         cgcATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAATCGTCGACTGGTAc                          <  1:1241098/150‑1 (MQ=255)
                                                                                                                          gcATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAATCGTCGACTGGTAcc                         <  1:905344/150‑1 (MQ=255)
                                                                                                                            aTCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAATCGTCGACTGGTACcgc                       >  1:679551/1‑150 (MQ=255)
                                                                                                                            aTCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAATCGTCGACTGGTACcgc                       >  2:8231202/1‑150 (MQ=255)
                                                                                                                              cgcgCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAATCGTCGACTGGTACCGCga                     >  2:7741535/1‑150 (MQ=255)
                                                                                                                               gcgcCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAATCGTCGACTGGTACCGCgag                    <  1:618156/150‑1 (MQ=255)
                                                                                                                                  ccGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAATCGTCGACTGGTACCGCGAGAAc                 >  1:3692413/1‑150 (MQ=255)
                                                                                                                                   cGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAATCGTCGACTGGTACCGCGAGAAcc                <  2:300895/150‑1 (MQ=255)
                                                                                                                                    ggAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAATCGTCGACTGGTACCGCGAGAACCa               >  2:7613708/1‑150 (MQ=255)
                                                                                                                                      aGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGTGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAATCGTCGACTGGTACCGCGAGAACCATg             <  1:4119723/150‑1 (MQ=255)
                                                                                                                                       gcgcgcGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAATCGTCGACTGGTACCGCGAGAACCATGc            <  1:3294774/150‑1 (MQ=255)
                                                                                                                                         gcgcgGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAATCGTCGACTGGTACCGCGAGAACCATGccc          >  2:327914/1‑150 (MQ=255)
                                                                                                                                           gcgGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAATCGTCGACTGGTACCGCGAGAACCATGCCCgg        <  1:2934236/150‑1 (MQ=255)
                                                                                                                                            cgGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAATCGTCGACTGGTACCGCGAGAACCATGCCCGgc       <  2:5550961/150‑1 (MQ=255)
                                                                                                                                              gCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAATCGTCGACTGGTACCGCGAGAACCATGCCCGgctc     <  1:4476276/150‑1 (MQ=255)
                                                                                                                                                 aacgaGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAATCGTCGACTGGTACCGCGAGAACCATGCCCGgctcgct  <  1:5548168/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
GCGTTCGTTCGCTGGAGCCTGGGCGTGCTGCTGATCGACATCCCGCTGCGGGTGTTCTCCTTCATGCAGGACCTGCCCAGCCACGACTTCCACCACCCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACAAGGCGCGCCCCTCAAAGTTCGGCCCGATGACCAAAACCTGGGGCATGTGGGAAAACTTCCTGATCCTGCGCAACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGG‑ATCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCT  >  NC_008463/3566568‑3566861

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑