BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_008463	3,670,384	A→G	Q128Q (CAA→CAG)	PA14_RS16675 →	ABC transporter permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_008463	3,670,384	0	A	G	99.8%	1620.9 / NA	415	Q128Q (CAA→CAG)	PA14_RS16675	ABC transporter permease
Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (207/206); total (209/206)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 415 total aligned reads displayed.

CCCCTACATCAGCCAACTGATCGCCGAGAAGGACGGCTGGGTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAAGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCA  >  NC_008463/3670237‑3670530

   cTACATCAGCCAACTGATCGCCGAGAAGGACGGCTGGGTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCcg                                                                                                                                               >  2:7582681/1‑150 (MQ=255)

   cTACATCAGCCAACTGATCGCCGAGAAGGACGGCTGGGTGCTTTGGCCGCCGATCCCATACAGCTACGACACCATCAACTACGAGCTGAATGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACAAGGCCcg                                                                                                                                               >  2:7909215/1‑150 (MQ=255)

       aTCAGCCAACTGATCGCCGAGAAGGACGGCTGGGTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGAc                                                                                                                                           <  1:2050822/150‑1 (MQ=255)

          aGCCAACTGATCGCCGAGAAGGACGGCTGGGTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTg                                                                                                                                        <  1:10222337/150‑1 (MQ=255)

             cAACTGATCGCCGAGAAGGACGGCTGGGTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTc                                                                                                                                     <  1:10562166/150‑1 (MQ=255)

              accTGATCGCCGAGACGGACGGCTGGGTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCg                                                                                                                                    <  2:6656079/148‑1 (MQ=255)

               aCTGATCGCCGAGAAGGACGGCTGGGTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGc                                                                                                                                   >  1:2995085/1‑150 (MQ=255)

                cTGATCGCCGAGAAGGACGGCTGGGTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGcc                                                                                                                                  <  2:9263395/150‑1 (MQ=255)

                  gATCGCCGAGAAGGACGGCTGGGTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCg                                                                                                                                <  2:9381591/150‑1 (MQ=255)

                     cGCCGAGAAGGACGGCTGGGTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGt                                                                                                                             <  2:505296/150‑1 (MQ=255)

                     cGCCGAGAAGGACGGCTGGGTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGt                                                                                                                             <  1:9092113/150‑1 (MQ=255)

                     cGCCGAGAAGGACGGCTGGGTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGt                                                                                                                             <  2:766903/150‑1 (MQ=255)

                      gCCGAGAAGGACGGCTGGGTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTg                                                                                                                            >  1:2627520/1‑150 (MQ=255)

                        cGAGAAGGACGGCTGGGTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGAt                                                                                                                          <  2:5566806/150‑1 (MQ=255)

                         gagaAGGACGGCTGGGTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGAtt                                                                                                                         >  1:5732175/1‑150 (MQ=255)

                          agaAGGACGGCTGGGTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGAttt                                                                                                                        <  1:1959023/150‑1 (MQ=255)

                            aaGGACGGCTGGGTGCTTTGGCCGCCGATCCCCTACCGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTAc                                                                                                                      <  2:2894649/150‑1 (MQ=255)

                            aaGGACGGCTGGGTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTAc                                                                                                                      >  2:8924258/1‑150 (MQ=255)

                                 cGGCTGGGTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCtt                                                                                                                 <  1:6808872/150‑1 (MQ=255)

                                 cGGCTGGGTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCAACGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTAACGGCtt                                                                                                                 >  2:3700876/1‑150 (MQ=255)

                                        gTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCgtg                                                                                                          <  1:3463620/150‑1 (MQ=255)

                                          gCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTc                                                                                                        <  2:4591814/150‑1 (MQ=255)

                                          gCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTc                                                                                                        >  1:7096084/1‑150 (MQ=255)

                                           cTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCg                                                                                                       <  1:9822219/150‑1 (MQ=255)

                                            tttGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGa                                                                                                      <  1:555023/150‑1 (MQ=255)

                                            tttGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGa                                                                                                      <  1:1205602/150‑1 (MQ=255)

                                             ttGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGAt                                                                                                     <  1:7312755/150‑1 (MQ=255)

                                              tGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTGCCGCGTGTCGATc                                                                                                    >  2:1552762/1‑150 (MQ=255)

                                                 ccgccgATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCctc                                                                                                 >  2:10386052/1‑150 (MQ=255)

                                                 ccgccgATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCctc                                                                                                 <  2:6969271/150‑1 (MQ=255)

                                                   gccgATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCtt                                                                                               <  2:2289427/150‑1 (MQ=255)

                                                    ccgATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTc                                                                                              <  2:142839/150‑1 (MQ=255)

                                                    ccgATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTc                                                                                              <  2:381076/150‑1 (MQ=255)

                                                     cgATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCg                                                                                             <  1:10020705/150‑1 (MQ=255)

                                                         ccccTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGccct                                                                                         >  1:464210/1‑150 (MQ=255)

                                                         ccccTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGccct                                                                                         <  1:309696/150‑1 (MQ=255)

                                                             tACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGccctgacc                                                                                     >  1:5318588/1‑150 (MQ=255)

                                                             tACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCGTCCGCGTGTCGATCCTCTTCGccctgacc                                                                                     <  1:4085962/150‑1 (MQ=255)

                                                               cAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGccctgaccct                                                                                   <  2:6553055/150‑1 (MQ=255)

                                                                 gCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGccctgaccctga                                                                                 >  2:4360130/1‑150 (MQ=255)

                                                                   tACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCctgaccctgacc                                                                               <  2:5332989/150‑1 (MQ=255)

                                                                    aCGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGGGATCGCCCGGGTGATTTGCGGCTGCGGCGTGTCGATCCTCTACGCCGTGTCCCTGATCg                                                                              >  2:9078822/1‑150 (MQ=25)

                                                                      gACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTg                                                                            >  1:7679394/1‑150 (MQ=255)

                                                                         acCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTc                                                                         >  2:324690/1‑150 (MQ=255)

                                                                             tCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGtt                                                                     >  1:6696703/1‑150 (MQ=255)

                                                                              cAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTtc                                                                    >  2:23833/1‑150 (MQ=255)

                                                                              cAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTtc                                                                    >  1:1751634/1‑150 (MQ=255)

                                                                                aCTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTtctc                                                                  <  1:8952915/150‑1 (MQ=255)

                                                                                aCTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTtctc                                                                  <  1:7788128/150‑1 (MQ=255)

                                                                                  tACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGGCGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGGGCTCAGTTCTCTg                                                                >  1:486294/1‑150 (MQ=255)

                                                                                  gaCGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTg                                                                <  1:38537/149‑1 (MQ=255)

                                                                                   aCGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGa                                                               >  1:8840099/1‑150 (MQ=255)

                                                                                    cGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGAt                                                              <  2:390763/150‑1 (MQ=255)

                                                                                     gAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATc                                                             <  1:1518311/150‑1 (MQ=255)

                                                                                     gAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATc                                                             >  1:6550549/1‑150 (MQ=255)

                                                                                      aGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCg                                                            >  1:5810805/1‑150 (MQ=255)

                                                                                      aGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCg                                                            <  1:9524411/150‑1 (MQ=255)

                                                                                      aGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCg                                                            >  2:6361368/1‑150 (MQ=255)

                                                                                       gCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCgg                                                           >  2:955646/1‑150 (MQ=255)

                                                                                        cTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGc                                                          >  1:8219441/1‑150 (MQ=255)

                                                                                          gAATGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAATCTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCTGTTCTCTGATCGGCGt                                                        <  2:3528136/150‑1 (MQ=255)

                                                                                           aaGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTc                                                       <  1:1695560/150‑1 (MQ=255)

                                                                                              gTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGTTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCAcc                                                    >  1:4967705/1‑150 (MQ=255)

                                                                                              gTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCAcc                                                    <  1:3314146/150‑1 (MQ=255)

                                                                                                 cccgcccccgccCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTc                                                 >  2:7472811/1‑150 (MQ=255)

                                                                                                     cccccgccccGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGgcg                                             >  2:1710507/1‑150 (MQ=255)

                                                                                                      ccccgccccgCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGgcgc                                            <  1:8565456/150‑1 (MQ=255)

                                                                                                       cccgccccgcCGTCGGCGGAAAACTGGCTGGGCACCGACGCCCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGcc                                           <  2:4088444/150‑1 (MQ=255)

                                                                                                       cccgccccgcCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGcc                                           <  1:6224438/150‑1 (MQ=255)

                                                                                                        ccgccccgccGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGccc                                          >  1:5865539/1‑150 (MQ=255)

                                                                                                        ccgccccgccGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGAGCTCGGCCGGGTGATTTTCGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGAGTCACAGTCGGCGccc                                          >  2:8251140/1‑150 (MQ=25)

                                                                                                         cgccccgccGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGGGCTCAGTTCTCGGAGCGGCGTCACCGTCGGCGCGCt                                         >  2:3217889/1‑150 (MQ=25)

                                                                                                          gccccgccGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTg                                        >  1:7497396/1‑150 (MQ=255)

                                                                                                           ccccgccGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGc                                       >  2:4968913/1‑150 (MQ=255)

                                                                                                              cgccgTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAg                                    >  2:10619146/1‑150 (MQ=255)

                                                                                                               gccgTCGGCGGAAAACTGGCTGGGCACCGCCGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAgg                                   <  2:8760039/150‑1 (MQ=255)

                                                                                                               gccgTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAgg                                   <  2:5715744/150‑1 (MQ=255)

                                                                                                                   tCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTGCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCTGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGctac                               >  2:2858598/1‑150 (MQ=255)

                                                                                                                    cggcggAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGctact                              >  1:9196790/1‑150 (MQ=255)

                                                                                                                      gcggAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCtactac                            >  2:6832795/1‑150 (MQ=255)

                                                                                                                       cggAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTAcg                           <  2:6004693/150‑1 (MQ=255)

                                                                                                                       cggAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTAcg                           >  1:10403211/1‑150 (MQ=255)

                                                                                                                        ggAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTAcgg                          >  2:1078820/1‑150 (MQ=255)

                                                                                                                         gAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTAcggc                         >  1:1662260/1‑150 (MQ=255)

                                                                                                                          aaaaCTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTAcggcg                        <  1:1313366/150‑1 (MQ=255)

                                                                                                                            aaCTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACggcggc                      >  2:6165621/1‑150 (MQ=255)

                                                                                                                             aCTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCt                     >  1:3703728/1‑150 (MQ=255)

                                                                                                                              ctggctggGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTg                    <  2:4427191/150‑1 (MQ=255)

                                                                                                                                   tggGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCg               <  2:8445120/150‑1 (MQ=255)

                                                                                                                                   tggGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCg               <  1:7975343/150‑1 (MQ=255)

                                                                                                                                       cACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCt           <  1:6387519/150‑1 (MQ=255)

                                                                                                                                         ccGACGACCAGGCCAGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGc         >  2:1793005/1‑150 (MQ=255)

                                                                                                                                          cgacgaCCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCt        <  1:2462459/150‑1 (MQ=255)

                                                                                                                                          cgacgaCCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGGGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCCAGGCTACTACGGCGGCTGGGTCGACCTGCt        >  2:5459388/1‑150 (MQ=255)

                                                                                                                                              gacCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGc    >  1:4433514/1‑150 (MQ=255)

                                                                                                                                               acCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGcc   >  2:7225084/1‑150 (MQ=255)

                                                                                                                                               acCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGcc   >  2:10172991/1‑150 (MQ=255)

                                                                                                                                               acCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGcc   >  2:7058521/1‑150 (MQ=255)

                                                                                                                                               acCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGcc   >  2:4829139/1‑150 (MQ=255)

                                                                                                                                                ccAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCa  >  2:1750901/1‑150 (MQ=255)

CCCCTACATCAGCCAACTGATCGCCGAGAAGGACGGCTGGGTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAAGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCA  >  NC_008463/3670237‑3670530

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`