Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 3,933,352 C→G P346P (CCC→CCG)  PA14_RS17955 → MFS transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084633,933,3520CG100.0% 2162.7 / NA 550P346P (CCC→CCG) PA14_RS17955MFS transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (247/303);  total (247/303)

Only 100 of 560 total aligned reads displayed.

GATCGTTCCCACCGCCTTCGAGGTAGCCGGCCTGCTCGGCCCGATCCTGATCGGCTTCGCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCCGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCGGACTCGATGATCTGCGGCTCCGCCGCCATCGACTTCGGCACCGGCGAGGCCGCCGGCACCGCCTCCGGCTTCGT  >  NC_008463/3933205‑3933498
                                                                                                                                                   |                                                                                                                                                  
gATCGTTCCCACCGCCTTCGAGGTAGCCGGCCTGCTCGGCCCGATCCTGATCGGCGTCGCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCggc                                                                                                                                                  <  2:6534329/150‑1 (MQ=255)
 aTCGTTCCCACCGCCTTCGAGGTAGCCGGCCTGCTCGGCCCGATCCTGATCGGCTTCGCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCATGGCGCTGTTCGTCACggcg                                                                                                                                                 >  1:2154586/1‑150 (MQ=255)
 aTCGTTCCCACCGCCTTCGAGGTAGCCGGCCTGCTCGGCCCGATCCTGATCGGCTTCGCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCGGCTGGCGCTGACCGTCTGCCTGGCGCTGTGCGTCCCggcg                                                                                                                                                 >  2:7065180/1‑150 (MQ=255)
  tCGTTCCCACCGCCTTCGAGGTAGCCGGCCTGCTCGGCCCGATCCTGATCGGCTTCGCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGa                                                                                                                                                >  1:9232725/1‑150 (MQ=255)
  acGTTCCCACCGCCTTCGAGGTAGCCGGCCTGCTCGGCCCGATCCTGATCGGATTCGCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGa                                                                                                                                                <  1:9728126/149‑1 (MQ=255)
    gTTCCCACCGCCTTCGAGGTAGCCGGCCTGCTCGGCCCGATCCTGATCGGCTTCGCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATg                                                                                                                                              <  1:286908/150‑1 (MQ=255)
     ttCCCACCGCCTTCGAGGTAGCCGGCCTGCTCGGCCCGATCCTGATCGGCTTCGCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGa                                                                                                                                             >  1:1426319/1‑150 (MQ=255)
       cccACCGCCTTCGAGGTAGCCGGCCTGCTCGGCCCGATCCTGATCGGCTTCGCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGacc                                                                                                                                           <  1:9969777/150‑1 (MQ=255)
        ccACCGCCTTCGAGGTAGCCGGCCTGCTCGGCCCGATCCTGATCGGCTTCGCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGacca                                                                                                                                          >  1:4205201/1‑150 (MQ=255)
          aCCGCCTTCGAGGTAGCCGGCCTGCTCGGCCCGATCCTGATCGGCTTCGCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGaccacc                                                                                                                                        >  2:3340036/1‑150 (MQ=255)
             gCCTTCGAGGTAGCCGGCCTGCTCGGCCCGATCCTGATCGGCTTCGCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGgc                                                                                                                                     <  2:2478654/150‑1 (MQ=255)
                 tCGAGGGAGCCGGCCTGCTCGGCCCGATCCTGATCGGCTTCGCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGgcagca                                                                                                                                 <  2:9624888/150‑1 (MQ=255)
                   gAGGTAGCCGGCCTGCTCGGCCCGATCCTGATCGGCTTCGCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCAtt                                                                                                                               >  2:1654171/1‑150 (MQ=255)
                   gAGGTAGCCGGCCTGCTCGGCCCGATCCTGATCGGCTTCGCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCAtt                                                                                                                               >  1:458581/1‑150 (MQ=255)
                     ggTAGCCGGCCTGCTCGGCCCGATCCTGATCGGCTTCGCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTcc                                                                                                                             >  2:8511940/1‑150 (MQ=255)
                     ggTAGCCGGCCTGCTCGGCCCGATCCTGATCGGCTTCGCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTcc                                                                                                                             <  2:2982081/150‑1 (MQ=255)
                     ggTAGCCGGCCTGCTCGGCCCGATCCTGATCGGCTTCGCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTcc                                                                                                                             >  1:1082874/1‑150 (MQ=255)
                       tAGCCGGCCTGCTCGGCCCGATCCTGATCGGCTTCGCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGa                                                                                                                           <  2:2244562/150‑1 (MQ=255)
                        aGCCGGCCTGCTCGGCCCGATCCTGATCGGCTTCGCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGAt                                                                                                                          >  1:562273/1‑150 (MQ=255)
                        aGCCGGCCTGCTCGGCCCGATCCTGATCGGCTTCGCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGAt                                                                                                                          <  1:1827202/150‑1 (MQ=255)
                         gCCGGCCTGCTCGGCCCGATCCTGATCGGCTTCGCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATa                                                                                                                         <  2:4197946/150‑1 (MQ=255)
                           cGGCCTGCTCGGCCCGATCCTGATCGGCTTCGCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGt                                                                                                                       <  2:6672770/150‑1 (MQ=255)
                           cGGCCTGCTCGGCCCGATCCTGATCGGCTTCGCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGt                                                                                                                       <  2:3936514/150‑1 (MQ=255)
                           cGGCCTGCTCGGCCCGATCCTGATCGGCTTCGCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGt                                                                                                                       <  1:9842404/150‑1 (MQ=255)
                              ccTGCTCGGCCCGATCCTGATCGGCTTCGCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGt                                                                                                                    <  2:10424293/150‑1 (MQ=255)
                              ccTGCTCGGCCCGATCCTGATCGGCTTCGCCTCGGACAAGCTGGTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGt                                                                                                                    <  2:5545196/150‑1 (MQ=255)
                               cTGCTCGGCCCGATCCTGATCGGCTTCGCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTc                                                                                                                   <  2:9052360/150‑1 (MQ=255)
                                 gCTCGGCCCGATCCTGATCGGCTTCGCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGCTAGTGGTCgg                                                                                                                 >  2:1264432/1‑150 (MQ=255)
                                 gCTCGGCCCGATCCTGATCGGCTTCGCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCgg                                                                                                                 >  1:10092543/1‑150 (MQ=255)
                                 gCTCGGCCCGATCCTGATCGGCTTCGCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCgg                                                                                                                 <  1:4982347/150‑1 (MQ=255)
                                   tCGGCCCGATCCTGATCGGCTTCGCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGcc                                                                                                               <  1:3254430/150‑1 (MQ=255)
                                        ccGATCCTGATCGGCTTCGCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCctgctg                                                                                                          >  2:6348143/1‑150 (MQ=255)
                                         cGATCCTGATCGGCTTCGCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCGTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGt                                                                                                         >  1:10344009/1‑150 (MQ=255)
                                         cGATCCTGATCGGCTTCGCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGt                                                                                                         >  2:9197469/1‑150 (MQ=255)
                                         cGATCCTGATCGGCTTCGCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGt                                                                                                         >  2:3436649/1‑150 (MQ=255)
                                         cGATCCTGATCGGCTTCGCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGt                                                                                                         <  2:2657511/150‑1 (MQ=255)
                                         cGATCCTGATCGGCTTCGCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGGCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTGCGATAGTGGTCGGCCTGCTGt                                                                                                         >  1:4099720/1‑150 (MQ=255)
                                            tCCTGATCGGCTTCGCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCa                                                                                                      <  1:903045/150‑1 (MQ=255)
                                             ccTGATCGGCTTCGCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCat                                                                                                     <  1:7420673/150‑1 (MQ=255)
                                                gATCGGCTTCGCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCatgat                                                                                                  >  1:3407448/1‑150 (MQ=255)
                                                  tCGGCTTCGCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATgg                                                                                                <  2:928766/150‑1 (MQ=255)
                                                   cGGCTTCGCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATggg                                                                                               >  2:4112159/1‑150 (MQ=255)
                                                   cGGCTTCGCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATggg                                                                                               >  1:5199287/1‑150 (MQ=255)
                                                       ttCGCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTg                                                                                           >  1:151952/1‑150 (MQ=255)
                                                       ttCGCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTg                                                                                           >  2:7576879/1‑150 (MQ=255)
                                                       ttCGCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTg                                                                                           >  2:6832591/1‑150 (MQ=255)
                                                          gCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGAcc                                                                                        >  2:3659613/1‑150 (MQ=255)
                                                          gCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGAcc                                                                                        <  2:2829886/150‑1 (MQ=255)
                                                           ccTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGAccc                                                                                       >  2:5631844/1‑150 (MQ=255)
                                                            cTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCt                                                                                      >  1:2389246/1‑150 (MQ=255)
                                                              cGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGt                                                                                    <  1:8949025/150‑1 (MQ=255)
                                                                    aGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGcc                                                                              >  2:3882941/1‑150 (MQ=255)
                                                                    aGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGcc                                                                              >  1:8790733/1‑150 (MQ=255)
                                                                      cTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCg                                                                            >  1:5368117/1‑150 (MQ=255)
                                                                       tGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCgg                                                                           >  1:6124083/1‑150 (MQ=255)
                                                                       tGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCgg                                                                           <  1:95591/150‑1 (MQ=255)
                                                                         ttCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCGGAc                                                                         >  1:5922028/1‑150 (MQ=255)
                                                                          tCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCGGACt                                                                        <  1:9321359/150‑1 (MQ=255)
                                                                           cggcgcacggcgcaTGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCGGACTc                                                                       <  1:8004671/150‑1 (MQ=255)
                                                                             gcgcacggcgcaTGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCGGACTCga                                                                     >  1:8424508/1‑150 (MQ=255)
                                                                              cgcacggcgcaTGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCGGACTCgat                                                                    >  2:4579875/1‑150 (MQ=255)
                                                                                  cggcgcaTGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCGGACTCGATGATc                                                                <  1:7127283/150‑1 (MQ=255)
                                                                                        aTGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCGGACTCGATGATCTGCGGc                                                          >  2:10188393/1‑150 (MQ=255)
                                                                                         tGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCGGACTCGATGATCTGCGGCt                                                         <  2:4463/150‑1 (MQ=255)
                                                                                             ggCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCGGACTCGATGATCTGCGGCTccgc                                                     <  2:870531/150‑1 (MQ=255)
                                                                                                cTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCGGACTCGATGATCTGCGGCTCcgccgc                                                  <  1:2013348/150‑1 (MQ=255)
                                                                                                cTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCGGACTCGATGATCTGCGGCTCcgccgc                                                  >  1:2593503/1‑150 (MQ=255)
                                                                                                    gTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCGGACTCGATGATCTGCGGCTCCGCCGCCATc                                              <  2:7269328/150‑1 (MQ=255)
                                                                                                     tGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCGGACTCGATGATCTGCGGCTCCGCCGCCATCg                                             >  2:51476/1‑150 (MQ=255)
                                                                                                         cAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCGGACTCGATGATCTGCGGCTCCGCCGCCATCGACtt                                         <  1:4820890/150‑1 (MQ=255)
                                                                                                           gCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCGGACTCGATGATCTGCGGCTCCGCCGCCATCGACTTCg                                       >  2:10612925/1‑150 (MQ=255)
                                                                                                           gCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCGGACTCGATGATCTGCGGCTCCGCCGCCATCGACTTCg                                       <  2:4718185/150‑1 (MQ=255)
                                                                                                            ccTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCGGACTCGATGATCTGCGGCTCCGCCGCCATCGACTTCgg                                      <  1:3759517/150‑1 (MQ=255)
                                                                                                             ctgctgGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCGGACTCGATGATCTGCGGCTCCGCCGCCATCGACTTCGGc                                     >  2:8177061/1‑150 (MQ=255)
                                                                                                             ctgctgGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGAACGGCCCGGACTCGATGATCTGCGGCTCCGCCGCCATCGACTTCGGc                                     >  2:3140546/1‑150 (MQ=255)
                                                                                                               gctgGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCGGACTCGATGATCTGCGGCTCCGCCGCCATCGACTTCGGCAc                                   <  1:8639054/150‑1 (MQ=255)
                                                                                                                 tgGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCGGACTCGATGATCTGCGGCTCCGCCGCCATCGACTTCGGCACCg                                 <  2:2373157/150‑1 (MQ=255)
                                                                                                                   gcgcTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCGGACTCGATGATCTGCGGCTCCGCCGCCATCGACTTCGGCACCGGc                               >  1:3806647/1‑150 (MQ=255)
                                                                                                                   gcgcTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCGGACTCGATGATCTGCGGCTCCGCCGCCATCGACTTCGGCACCGGc                               <  2:5352291/150‑1 (MQ=255)
                                                                                                                       tGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCGGACTCGATGATCTGCGGCTCCGCCGCCATCGACTTCGGCACCGGCGAgg                           >  1:10531942/1‑150 (MQ=255)
                                                                                                                           cGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCGGACTCGATGATCTGCGGCTCCGCCGCCATCGACTTCGGCACCGGCGAGgccgc                       <  2:10003217/150‑1 (MQ=255)
                                                                                                                             tCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCGGACTCGATGATCTGCGGCTCCGCCGCCATCGACTTCGGCACCGGCGAGGccgccg                     >  1:1005058/1‑150 (MQ=255)
                                                                                                                              cTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCGGACTCGATGATCTGCGGCTCCGCCGCCATCGACTTCGGCACCGGCGAGGCCGCCgg                    >  2:1984067/1‑150 (MQ=255)
                                                                                                                               tGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCGGACTCGATGATCTGCGGCTCCGCCGCCATCGACTTCGGCACCGGCGAGGCCGCCGGc                   <  2:6133991/150‑1 (MQ=255)
                                                                                                                               tGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCGGACTCGATGATCTGCGGCTCCGCCGCCATCGACTTCGGCACCGGCGAGGCCGCCGGc                   >  1:7523666/1‑150 (MQ=255)
                                                                                                                               tGCCTGGCGCTGGTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCGGACTCGATGATCTGCGGCTCCGCCGCCATCGACTTCGGCACCGGCGAGGCCGCCGGc                   <  2:1436685/150‑1 (MQ=255)
                                                                                                                                gCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCGGACTCGATGATCTGCGGCTCCGCCGCCATCGACTTCGGCACCGGCGAGGCCGCCGGCa                  >  2:5941582/1‑150 (MQ=255)
                                                                                                                                gCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCGGACTCGATGATCTGCGGCTCCGCCGCCATCGACTTCGGCACCGGCGAGGCCGCCGGCa                  <  1:6976537/150‑1 (MQ=255)
                                                                                                                                   tGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCGGACTCGATGATCTGCGGCTCCGCCGCCATCGACTTCGGCACCGGCGAGGCCGCCGGCACCg               >  2:703382/1‑150 (MQ=255)
                                                                                                                                   tGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCGGACTCGATGATCTGCGGCTCCGCCGCCATCGACTTCGGCACCGGCGAGGCCGCCGGCACCg               >  1:6291986/1‑150 (MQ=255)
                                                                                                                                       gcTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCGGACTCGATGATCTGCGGCTCCGCCGCCATCGACTTCGGCACCGGCGAGGCCGCCGGCACCGCCTc           <  2:5791522/150‑1 (MQ=255)
                                                                                                                                       gcTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCGGACTCGATGATCTGCGGCTCCGCCGCCATCGACTTCGGCACCGGCGAGGCCGCCGGCACCGCCTc           <  2:4874297/150‑1 (MQ=255)
                                                                                                                                       gcTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCGGACTCGATGATCTGCGGCTCCGCCGCCATCGACTTCGGCACCGGCGAGGCCGCCGGCACCGCCTc           <  1:4385793/150‑1 (MQ=255)
                                                                                                                                       gcTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCGGACTCGATGATCTGCGGCTCCGCCGCCATCGACTTCGGCACCGGCGAGGCCGCCGGCACCGCCTc           <  2:10385944/150‑1 (MQ=255)
                                                                                                                                       gcTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCGGACTCGATGATCTGCGGCTCCGCCGCCATCGACTTCGGCACCGGCGAGGCCGCCGGCACCGCCTc           <  2:10074017/150‑1 (MQ=255)
                                                                                                                                         tGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCGGACTCGATGATCTGCGGCTCCGCCGCCATCGACTTCGGCACCGGCGAGGCCGCCGGCACCGCCTCCg         >  2:7995108/1‑150 (MQ=255)
                                                                                                                                            tCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCGGACTCGATGATCTGCGGCTCCGCCGCCATCGACTTCGGCACCGGCGAGGCCGCCGGCACCGCCTCCGGCt      <  2:2547393/150‑1 (MQ=255)
                                                                                                                                              gTCCCGGCGATGACCACCGGCAGCAGTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCGGACTCGATGATCTGCGGCTCCGCCGCCATCGACTTCGGCACCGGCGAGGCCGCCGGCACCGCCTCCGGCTTc    <  1:824798/150‑1 (MQ=255)
                                                                                                                                               tCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCGGACTCGATGATCTGCGGCTCCGCCGCCATCGACTTCGGCACCGGCGAGGCCGCCGGCACCGCCTCCGGCTTCg   >  1:6652735/1‑150 (MQ=255)
                                                                                                                                                cccgGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCGGACTCGATGATCTGCGGCTCCGCCGCCATCGACTTCGGCACCGGCGAGGCCGCCGGCACCGCCTCCGGCTTCGt  <  2:8285984/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GATCGTTCCCACCGCCTTCGAGGTAGCCGGCCTGCTCGGCCCGATCCTGATCGGCTTCGCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCCGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCGGACTCGATGATCTGCGGCTCCGCCGCCATCGACTTCGGCACCGGCGAGGCCGCCGGCACCGCCTCCGGCTTCGT  >  NC_008463/3933205‑3933498

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑