Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 4,659,805 A→G intergenic (+45/+54) alpR → / ← PA14_RS21395 transcriptional regulator AlpR/tRNA‑Arg

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084634,659,8050AG100.0% 1407.4 / NA 359intergenic (+45/+54)alpR/PA14_RS21395transcriptional regulator AlpR/tRNA‑Arg
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (162/197);  total (162/197)

Only 100 of 373 total aligned reads displayed.

CTTCAACAGCGACGAGCACCCGGACGAGCGCTACGAGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAAAAAAATCCCGGGCAAAAAAAAAGGGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTGCGTGTCGGTGCTTTTTCC  >  NC_008463/4659658‑4659952
                                                                                                                                                   |                                                                                                                                                   
cTTCAACAGCGACGAGCACCCGGACGAGCGCTACGAGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGgaagaa                                                                                                                                                   >  1:8896691/1‑150 (MQ=255)
cTTCAACAGCGACGAGCACCCGGACGAGCGCTACGAGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGgaagaa                                                                                                                                                   <  2:251636/150‑1 (MQ=255)
  tCAACAGCGACGAGCACCCGGACGAGCGCTACGAGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGaagaaaa                                                                                                                                                 >  2:4043941/1‑150 (MQ=255)
      cAGCGACGAGCACCCGGACGAGCGCTACGAGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATcc                                                                                                                                             >  1:4779558/1‑150 (MQ=255)
       aGCGACGAGCACCCGGACGAGCGCTACGAGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATccc                                                                                                                                            <  1:1634081/150‑1 (MQ=255)
        gCGACGAGCACCCGGACGAGCGCTACGAGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCg                                                                                                                                           <  2:7377529/150‑1 (MQ=255)
           acgaGCACCCGGACGAGCGCTACGAGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGc                                                                                                                                        <  2:460831/150‑1 (MQ=255)
             gaGCACCCGGACGAGCGCTACGAGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCaa                                                                                                                                      <  1:7325469/150‑1 (MQ=255)
              aGCACCCGGACGAGCGCTACGAGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCaaa                                                                                                                                     >  1:9563480/1‑150 (MQ=255)
              aGCACCCGGACGAGCGCTACGAGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCaaa                                                                                                                                     >  1:5662963/1‑150 (MQ=255)
               gCACCCGGACGAGCGCTACGAGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCaaag                                                                                                                                    >  1:2616502/1‑149 (MQ=255)
                  cccGGACGAGCGCTACGAGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCaaagaaa                                                                                                                                 >  2:3568959/1‑150 (MQ=255)
                      gACGAGCGCTACGAGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGACAAATCCCGGGCAAAGAAAAAgg                                                                                                                             >  2:8266421/1‑150 (MQ=255)
                      gACGAGCGCTACGAGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAAgg                                                                                                                             >  1:2120531/1‑150 (MQ=255)
                       aCGAGCGCTACGAGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCGTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAAggg                                                                                                                            <  1:5064550/150‑1 (MQ=255)
                        cGAGCGCTACGAGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAAgggc                                                                                                                           <  1:4680969/150‑2 (MQ=255)
                        cGAGCGCTACGAGCCCCACGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTGCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAAgggc                                                                                                                           <  2:1256470/150‑2 (MQ=255)
                         gAGCGCTACGAGCCCCAGGAAGCCGCCGAGCACATCACCGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAAgggcc                                                                                                                          <  2:2076412/150‑3 (MQ=255)
                         gAGCGCTACGAGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAATAgggcg                                                                                                                          >  1:1097693/1‑148 (MQ=255)
                         gAGCGCGACGAGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAAgggcc                                                                                                                          <  2:4786028/150‑3 (MQ=255)
                              cTACGAGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCAt                                                                                                                    >  2:766628/1‑150 (MQ=255)
                              cTACGAGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCAt                                                                                                                    >  2:2240787/1‑150 (MQ=255)
                              cTACGAGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCAt                                                                                                                    >  1:9436234/1‑150 (MQ=255)
                                  gAGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATtgct                                                                                                                <  1:9958902/150‑1 (MQ=255)
                                   aGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGTTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGC‑AAATAAAATGGGCCTGCATtgctg                                                                                                               >  2:1208133/1‑150 (MQ=255)
                                   aGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTACCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATtggtg                                                                                                               >  1:10067788/1‑150 (MQ=255)
                                      cccAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAg                                                                                                            >  1:2234923/1‑150 (MQ=255)
                                        cAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGc                                                                                                          <  1:1842961/150‑1 (MQ=255)
                                             aGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCttt                                                                                                     <  1:6421228/150‑1 (MQ=255)
                                             aGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGGTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCttt                                                                                                     <  1:10025663/150‑1 (MQ=255)
                                                  ccgAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTa                                                                                                <  1:3523584/150‑1 (MQ=255)
                                                  ccgAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTa                                                                                                >  1:6874032/1‑150 (MQ=255)
                                                    gAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATg                                                                                              >  2:5092938/1‑150 (MQ=255)
                                                     aGCACCTCAACGCGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATgg                                                                                             <  2:8815492/150‑1 (MQ=255)
                                                     aGCACATCAACGTGATCGGCCGGGTGGTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATgg                                                                                             <  2:5215724/150‑1 (MQ=255)
                                                        acaTCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGgcgc                                                                                          <  1:5504005/150‑1 (MQ=255)
                                                           tccaCGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACt                                                                                       <  2:3009056/147‑1 (MQ=255)
                                                             aaCGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAACTCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCa                                                                                     >  2:5449574/1‑150 (MQ=255)
                                                               cGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCTCTCagg                                                                                   >  2:7253710/1‑150 (MQ=255)
                                                               cGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCagg                                                                                   >  2:7077939/1‑150 (MQ=255)
                                                                 tGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCaggag                                                                                 >  1:9288660/1‑150 (MQ=255)
                                                                  gATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGC‑AAAGAAAATGGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCaggagg                                                                                >  1:101372/1‑150 (MQ=255)
                                                                     cGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGAtt                                                                             <  2:9767889/150‑1 (MQ=255)
                                                                      ggCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAATTCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTc                                                                            >  1:10407/1‑150 (MQ=255)
                                                                      ggCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTc                                                                            <  1:8664640/150‑1 (MQ=255)
                                                                       gCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCg                                                                           <  1:3653935/150‑1 (MQ=255)
                                                                        ccGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGa                                                                          >  1:4925254/1‑150 (MQ=255)
                                                                         cGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGaa                                                                         >  2:167650/1‑150 (MQ=255)
                                                                         cGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGGTCCGGCGGAAGAAAAATCCCGGGCAAAGATAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGaa                                                                         <  2:3219160/150‑1 (MQ=255)
                                                                           ggTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAAcc                                                                       >  1:9703588/1‑150 (MQ=255)
                                                                                  tGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGAcc                                                                <  1:1243891/150‑1 (MQ=255)
                                                                                   ggTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGC‑AAAGAAAATGGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCg                                                               >  1:6183201/1‑150 (MQ=255)
                                                                                     tACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCt                                                             >  2:495420/1‑150 (MQ=255)
                                                                                     tACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCt                                                             >  2:9150242/1‑150 (MQ=255)
                                                                                      aCTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTc                                                            <  2:8047778/150‑1 (MQ=255)
                                                                                          ggTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGtt                                                        >  2:7852694/1‑150 (MQ=255)
                                                                                          ggTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGtt                                                        <  1:4392471/150‑1 (MQ=255)
                                                                                          ggTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGtt                                                        <  2:10517459/150‑1 (MQ=255)
                                                                                           gTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTc                                                       <  1:6062677/150‑1 (MQ=255)
                                                                                             gCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGt                                                     >  1:10574609/1‑150 (MQ=255)
                                                                                             gCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGt                                                     >  1:9870367/1‑150 (MQ=255)
                                                                                              cTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCCAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGGATGGCGCACTCAGGAGGATGCGAACCTCCGACCGCTCGGTTCGtt                                                    >  2:5988222/1‑149 (MQ=38)
                                                                                                    tGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAg                                              <  1:4416504/150‑1 (MQ=255)
                                                                                                    tGACCCCGTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAg                                              <  2:7645820/150‑1 (MQ=255)
                                                                                                     gACCCCGTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGt                                             <  2:6700130/150‑1 (MQ=255)
                                                                                                      aCCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTa                                            <  1:10211138/150‑1 (MQ=255)
                                                                                                      aCCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTa                                            <  2:9292184/150‑1 (MQ=255)
                                                                                                       ccccTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTAc                                           <  2:8751463/150‑1 (MQ=255)
                                                                                                         ccTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑TGGCCTGCATTGCTGCAGGCCCGTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCACGGTTCGTAGCCGAGTActc                                         >  2:1806139/1‑150 (MQ=37)
                                                                                                         ccTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTTGCCGAGTActc                                         >  1:8235083/1‑150 (MQ=255)
                                                                                                         ccTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTActc                                         <  2:3675863/150‑1 (MQ=255)
                                                                                                         ccTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTActc                                         >  1:6575508/1‑150 (MQ=255)
                                                                                                           ttCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTa                                       <  1:3817260/150‑1 (MQ=255)
                                                                                                           ttCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTa                                       <  1:3138162/150‑1 (MQ=255)
                                                                                                            gcccgccccgcCTGACGACGGGGCCGCGGTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCAGTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGATGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTActctct                                      <  2:7471476/149‑3 (MQ=37)
                                                                                                              ccgccccgccTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAACA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATcc                                    <  1:2752543/150‑1 (MQ=255)
                                                                                                              ccgccccgccTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATcc                                    <  1:7598885/150‑1 (MQ=255)
                                                                                                              ccgccccgacTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATcc                                    <  1:9213783/150‑1 (MQ=255)
                                                                                                               cgccccgccTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCa                                   <  1:3990144/150‑1 (MQ=255)
                                                                                                               cgccccgccTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCa                                   >  1:6287159/1‑150 (MQ=255)
                                                                                                                gccccgccTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTGTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAg                                  >  1:7994096/1‑150 (MQ=255)
                                                                                                                  cccgccTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCt                                >  1:1505636/1‑150 (MQ=255)
                                                                                                                  cccgccTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCt                                >  2:10072016/1‑150 (MQ=255)
                                                                                                                     gccTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAg                             <  1:5190615/150‑1 (MQ=255)
                                                                                                                       cTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGC‑AAAGAAAATGGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCt                           >  1:4621489/1‑150 (MQ=255)
                                                                                                                        tGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTa                          <  1:10490611/150‑1 (MQ=255)
                                                                                                                        tGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGGACTCTATCCAGCTGAGCTa                          <  2:2576027/150‑1 (MQ=255)
                                                                                                                         gacgacGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGCGCAAAGAAACA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTAt                         <  2:6487817/150‑1 (MQ=25)
                                                                                                                           cgacgGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGa                       <  1:7180329/150‑1 (MQ=255)
                                                                                                                             acgGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGt                     <  2:4225598/150‑1 (MQ=255)
                                                                                                                             aaggggCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACATCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGt                     >  2:6319012/3‑150 (MQ=25)
                                                                                                                                  gCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCGTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTGCgtg                >  1:7262169/1‑150 (MQ=255)
                                                                                                                                      cgTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTGCgtcgtgg            <  1:2328309/150‑6 (MQ=21)
                                                                                                                                        ttCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTGCGTGTCGGTg          >  2:1382175/1‑150 (MQ=255)
                                                                                                                                        ttCCGGCGGAAGAAAAAGCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTCTGGCGCACTCTGGTGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTGCGTGGCGGTg          >  2:9086312/1‑150 (MQ=21)
                                                                                                                                          ccGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTGCGTGTCGGTGCt        <  1:7655315/150‑1 (MQ=255)
                                                                                                                                            ggcggAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTGCGTGTCGGTGCttt      <  1:2462671/150‑1 (MQ=255)
                                                                                                                                             gcggTAGAAAAATCCCGGGCAAAGATATA‑GGTCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCTCTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTGAGTGTCGGTGCtttt     <  2:8497725/150‑1 (MQ=21)
                                                                                                                                             gcggAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTGCGTGGCGGTGCtttt     >  2:10016787/1‑150 (MQ=255)
                                                                                                                                                gaagaaAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTGCGTGTCGGTGCTTTTTcc  <  1:8399021/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
CTTCAACAGCGACGAGCACCCGGACGAGCGCTACGAGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAAAAAAATCCCGGGCAAAAAAAAAGGGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTGCGTGTCGGTGCTTTTTCC  >  NC_008463/4659658‑4659952

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑