Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 4,947,683 A→G H166R (CAC→CGC)  PA14_RS22670 → DNA polymerase Y family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084634,947,6830AG100.0% 1400.0 / NA 358H166R (CAC→CGC) PA14_RS22670DNA polymerase Y family protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (205/153);  total (205/153)

Only 100 of 358 total aligned reads displayed.

GGCCACGCTTCGAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAAGGTCAT‑GCGCACTACACAGGACAACATTTCCATCGAGGACGAGACAGCGCTGCAACACCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGACTGCGCCATCTGCGCCAGGTCCTCGAGTTGCCGCGCGCCGCCCTGGCCCGTCGCTTCCCGGCCGAACTGCTGT  >  NC_008463/4947536‑4947829
                                                                                                                                                    |                                                                                                                                                  
 gCCACGCTTCGAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCa                                                                                                                                                 >  2:8577196/1‑150 (MQ=255)
   cACGCTTCGAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGt                                                                                                                                               >  2:2916621/1‑150 (MQ=255)
    aCGCTTCGAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGtt                                                                                                                                              <  1:5892322/150‑1 (MQ=255)
         tCGAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCg                                                                                                                                         >  2:4282061/1‑150 (MQ=255)
         tCGAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCg                                                                                                                                         <  2:4616305/150‑1 (MQ=255)
         tCGAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCg                                                                                                                                         >  2:9627028/1‑150 (MQ=255)
          cGAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGCAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGc                                                                                                                                        >  2:6500964/1‑150 (MQ=255)
           gAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGcc                                                                                                                                       >  1:10554772/1‑150 (MQ=255)
           gAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGcc                                                                                                                                       >  1:899590/1‑150 (MQ=255)
            aGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGccc                                                                                                                                      >  1:1460451/1‑150 (MQ=255)
            aGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGccc                                                                                                                                      >  2:8771339/1‑150 (MQ=255)
             ggCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCt                                                                                                                                     <  2:1123042/150‑1 (MQ=255)
              gCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCtt                                                                                                                                    >  1:287802/1‑150 (MQ=255)
              gCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCtt                                                                                                                                    >  1:1228041/1‑150 (MQ=255)
               cccGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTc                                                                                                                                   >  1:4230248/1‑150 (MQ=255)
                    tggcgcAAGGAACTCACGGCGCTGGGCGTCCGCCCTCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTg                                                                                                                              <  1:5988323/148‑1 (MQ=255)
                      gcgcAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGa                                                                                                                            <  1:9708753/150‑1 (MQ=255)
                      gcgcAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGa                                                                                                                            <  1:5010989/150‑1 (MQ=255)
                        gcAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGAcc                                                                                                                          >  1:273752/1‑150 (MQ=255)
                        gcAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGAcc                                                                                                                          >  1:6816138/1‑150 (MQ=255)
                             gAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTg                                                                                                                     >  2:2307348/1‑150 (MQ=255)
                              aaCTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTgg                                                                                                                    >  2:1389286/1‑150 (MQ=255)
                              aaCTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGAAGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTgg                                                                                                                    <  1:4482081/150‑1 (MQ=255)
                                cTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGa                                                                                                                  >  2:3928932/1‑150 (MQ=255)
                                 tCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGAc                                                                                                                 >  2:1278182/1‑150 (MQ=255)
                                 tCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGAc                                                                                                                 >  1:4337866/1‑150 (MQ=255)
                                 tCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGAc                                                                                                                 >  2:5936016/1‑150 (MQ=255)
                                  cACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGAct                                                                                                                <  1:1734240/150‑1 (MQ=255)
                                   aCGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGActc                                                                                                               >  1:9339693/1‑150 (MQ=255)
                                     ggCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCCACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACtctc                                                                                                             >  2:5513550/1‑150 (MQ=255)
                                     ggCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACtctc                                                                                                             <  2:44866/150‑1 (MQ=255)
                                      gcgcTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTcg                                                                                                            >  1:9529133/1‑150 (MQ=255)
                                       cgcTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCACCGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTcgc                                                                                                           >  1:5698140/1‑150 (MQ=255)
                                        gcTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTcgcg                                                                                                          <  1:5368584/150‑1 (MQ=255)
                                         cTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCgg                                                                                                         >  1:10652710/1‑150 (MQ=255)
                                                 ccGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGc                                                                                                 >  1:677723/1‑150 (MQ=255)
                                                  cGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGcc                                                                                                >  1:8678187/1‑150 (MQ=255)
                                                   gCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCg                                                                                               >  2:7539865/1‑150 (MQ=255)
                                                     cATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCGTCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTc                                                                                             <  2:7246262/150‑1 (MQ=255)
                                                      aTCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGTGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCg                                                                                            >  2:350591/1‑150 (MQ=255)
                                                      aTCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCg                                                                                            >  2:257650/1‑150 (MQ=255)
                                                      aTCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCg                                                                                            >  2:9461510/1‑150 (MQ=255)
                                                       tCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGc                                                                                           <  1:4432243/150‑1 (MQ=255)
                                                       tCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGc                                                                                           >  2:4166282/1‑150 (MQ=255)
                                                          ccTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCt                                                                                        <  2:5287645/150‑1 (MQ=255)
                                                          ccTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCt                                                                                        <  2:7185706/150‑1 (MQ=255)
                                                          ccTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCt                                                                                        >  1:4684708/1‑150 (MQ=255)
                                                           cTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTc                                                                                       >  1:9747241/1‑150 (MQ=255)
                                                            tgtgCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCg                                                                                      >  1:4624523/1‑150 (MQ=255)
                                                            tgtgCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCg                                                                                      >  2:2715065/1‑150 (MQ=255)
                                                              tgCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGa                                                                                    >  2:7056958/1‑150 (MQ=255)
                                                              tgCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCT‑GGCGACCAGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGa                                                                                    <  2:6702735/150‑1 (MQ=255)
                                                               gCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGTACTCTCGCGGGAGCTGGCCGTCGCCCTCGGAc                                                                                   >  1:1954176/1‑150 (MQ=255)
                                                               gCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGAc                                                                                   <  2:9717584/150‑1 (MQ=255)
                                                               gCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGAc                                                                                   <  2:9060257/150‑1 (MQ=255)
                                                                  tGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCa                                                                                >  1:3998177/1‑150 (MQ=255)
                                                                   ggCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCAt                                                                               >  2:5179377/1‑150 (MQ=255)
                                                                    gcgcCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATg                                                                              <  2:5818139/150‑1 (MQ=255)
                                                                      gcCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATggg                                                                            <  2:1739233/150‑1 (MQ=255)
                                                                        cGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGAc                                                                          >  1:7673998/1‑150 (MQ=255)
                                                                        cGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGAc                                                                          <  2:3134151/150‑1 (MQ=255)
                                                                          aaCCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGACTg                                                                        >  1:8423424/1‑150 (MQ=255)
                                                                           aCCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGACTgc                                                                       <  2:2210882/150‑1 (MQ=255)
                                                                           aCCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGACTgc                                                                       >  2:3807662/1‑150 (MQ=255)
                                                                                                                  ggcctggccATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGACTGCGCCATCTGCGCCAGGTCCTCGAGTTGCCGCGCgccgcc                                 >  2:10508542/8‑150 (MQ=255)
                                                                                                                  ggcctggccATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGACGCCCTCGGACGCATGGGACTGCGCCATCTGCGCCAGGTCCTCGCGTTGCCGCGCgccgcc                                 >  1:9811206/8‑150 (MQ=255)
                                                                                                                   gcctggccATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGACTGCGCCATCTGCGCCAGGTCCTCGAGTTGCCGCGCGCCGccc                                >  1:10400808/7‑150 (MQ=255)
                                                                                                                   gcctggccATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGACTGCGCCATCTGCGCCAGGTCCTCGAGTTGCCGCGCGCCGccc                                <  1:3717938/144‑1 (MQ=255)
                                                                                                                   gcctggccATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGACTGCGCCATCTGCGCCAGGTCCTCGAGTTGCCGCGCGCCGccc                                >  1:2221121/7‑150 (MQ=255)
                                                                                                                    cctggccATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGACTGCGCCATCTGCGCCAGGTCCTCGAGTTGCCGCGCGCCGCCCt                               <  2:10121159/145‑1 (MQ=255)
                                                                                                                     ctggccATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGACTGCGCCATCTGCGCCAGGTCCTCGAGTTGCCGCGCGCCGCCCTg                              >  2:1195681/5‑150 (MQ=255)
                                                                                                                      tggccATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGACTGCGCCATCTGCGCCAGGTCCTCGAGTTGCCGCGCGCCGCCCTgg                             <  2:7349014/147‑1 (MQ=255)
                                                                                                                      tggccATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGACTGCGCCATCTGCGCCAGGTCCTCGAGTTGCCGCGCGCCGCCCTgg                             >  2:8953201/4‑150 (MQ=255)
                                                                                                                      tggccATCGAGGACGAGACAGCGCTGCAACGCCAGTGGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGACTGCGCCATCTGCGCCAGGTCCTCGAGTTGCCGCGCGCCGCCCTgg                             <  2:7992854/147‑1 (MQ=255)
                                                                                                                        gccATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCGCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGACTGCGCCATCTGCGCCAGGTCCTCGAGTTGCCGCGCGCCGCCCTGGcc                           >  1:5244177/2‑150 (MQ=255)
                                                                                                                        gccATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGACTGCGCCATCTGCGCCAGGTCCTCGAGTTGCCGCGCGCCGCCCTGGcc                           <  2:3607064/149‑1 (MQ=255)
                                                                                                                        gccATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGACTGCGCCATCTGCGCCAGGTCCTCGAGTTGCCGCGCGCCGCCCTGGcc                           <  1:2192848/149‑1 (MQ=255)
                                                                                                                         ccATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGACTGCGCCATCTGCGCCAGGTCCTCGAGTTGCCGCGCGCCGCCCTGGccc                          <  1:7608843/150‑1 (MQ=255)
                                                                                                                           aTCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGACTGCGCCATCTGCGCCAGGTCCTCGAGTTGCCGCGCGCCGCCCTGGCCCGt                        <  1:6498162/150‑1 (MQ=255)
                                                                                                                              gAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGACTGCGCCATCTGCGCCAGGTCCTCGAGTTGCCGCGCGCCGCCCTGGCCCGTCGc                     >  1:35591/1‑150 (MQ=255)
                                                                                                                              gAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGACTGCGCCATCTGCGCCAGGTCCTCGAGTTGCCGCGCGCCGCCCTGGCCCGTCGc                     <  1:3410348/150‑1 (MQ=255)
                                                                                                                              gAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGACTGCGCCATCTGCGCCAGGTCCTCGAGTTGCCGCGCGCCGCCCTGGCCCGTCGc                     <  2:849790/150‑1 (MQ=255)
                                                                                                                                ggACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGCCTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGACTGCGCCATCTGCGCCAGGTCCTCGAGTTGCCGCGCGCCGCCCTGGCCCGTCGCtt                   <  1:4097014/150‑1 (MQ=255)
                                                                                                                                 gACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGACTGCGCCATCTGCGCCAGGTCCTCGAGTTGCCGCGCGCCGCCCTGGCCCGTCGCTTc                  >  1:7953480/1‑150 (MQ=255)
                                                                                                                                 gACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGACTGCGCCATCTGCGCCAGGTCCTCGAGTTGCCGCGCGCCGCCCTGGCCCGTCGCTTc                  >  1:823700/1‑150 (MQ=255)
                                                                                                                                    gagaCAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGACTGCGCCATCTGCGCCAGGTCCTCGAGTTGCCGCGCGCCGCCCTGGCCCGTCGCTTCCCg               >  1:3182265/1‑150 (MQ=255)
                                                                                                                                    gagaCAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGACTGCGCCATCTGCGCCAGGTCCTCGAGTTGCCGCGCGCCGCCCTGGCCCGTCGCTTCCCg               >  2:8008442/1‑150 (MQ=255)
                                                                                                                                     agaCAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGACTGCGCCATCTGCTCCAGGTCCTCGAGTTGCCGCGCGCCGCCCTGGCCCGTCGCTTCCCgg              >  2:10293544/1‑150 (MQ=255)
                                                                                                                                     agaCAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGACTGCGCCATCTGCGCCAGGTCCTCGAGTTGCCGCGCGCCGCCCTGGCCCGTCGCTTCCCgg              >  1:7786351/1‑150 (MQ=255)
                                                                                                                                     agaCAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGACTGCGCCATCTGCGCCAGGTCCTCGAGTTGCCGCGCGCCGCCCTGGCCCGTCGCTTCCCgg              >  2:8108220/1‑150 (MQ=255)
                                                                                                                                     agaCAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCAACTGACGCATGGGCCAGCGCCCTATCCGCCAGGTCCTCTCGTAACAGCGAGCAGACCTGACCCGTCGAATCCCgg              >  2:3594711/1‑150 (MQ=255)
                                                                                                                                      gaCAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGACTGCGCCATCTGCGCCAGGTCCTCGAGTTGCCGCGCGCCGCCCTGGCCCGTCGCTTCCCGGc             <  2:3962454/150‑1 (MQ=255)
                                                                                                                                       aCAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGACTGCGCCATCTGCGCCAGGTCCTCGAGTTGCCGCGCGCCGCCCTGGCCCGTCGCTTCCCGGcc            <  2:7806330/150‑1 (MQ=255)
                                                                                                                                       aCAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGACTGCGCCATCTGCGCCAGGTCCTCGAGTTGCCGCGCGCCGCCCTGGCCCGTCGCTTCCCGGcc            >  1:1875133/1‑150 (MQ=255)
                                                                                                                                        cAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGCCTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGACTGCGCCATCTGCGCCAGGTCCTCGAGTTGCCGCGCGCCGCCCTGGCCCGTCGCTTCCCGGCCg           >  2:6953695/1‑150 (MQ=255)
                                                                                                                                          gcgcTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGACTGCGCCATCTGCGCCAGGTCCTCGAGTTGCCGCGCGCCGCCCTGGCCCGTCGCTTCCCGGCCGaa         <  1:1005207/150‑1 (MQ=255)
                                                                                                                                           cgcTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGACTGCGCCATCTGCGCCAGGTCCTCGAGTTGCCGCGCGCCGCCCTGGCCCGTCGCTTCCCGGCCGAAc        <  1:5717178/150‑1 (MQ=255)
                                                                                                                                           cgcTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGACTGCGCCATCTGCGCCAGGTCCTCGAGTTGCCGCGCGCCGCCCTGGCCCGTCGCTTCCCGGCCGAAc        >  1:9880512/1‑150 (MQ=255)
                                                                                                                                            gcTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGACTGCGCCATCTGCGCCAGGTCCTCGAGTTGCCGCGCGCCGCCCTGGCCCGTCGCTTCCCGGCCGAAct       <  2:8293025/150‑1 (MQ=255)
                                                                                                                                              tGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGACTGCGCCATCTGCGCCAGGTCCTCGAGTTGCCGCGCGCCGCCCTGGCCCGTCGCTTCCCGGCCGAActgc     >  2:6153087/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
GGCCACGCTTCGAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAAGGTCAT‑GCGCACTACACAGGACAACATTTCCATCGAGGACGAGACAGCGCTGCAACACCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGACTGCGCCATCTGCGCCAGGTCCTCGAGTTGCCGCGCGCCGCCCTGGCCCGTCGCTTCCCGGCCGAACTGCTGT  >  NC_008463/4947536‑4947829

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑