BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_008463	5,256,376	T→C	F87L (TTC→CTC)	dnaB →	replicative DNA helicase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_008463	5,256,376	0	T	C	99.7%	1316.4 / NA	338	F87L (TTC→CTC)	dnaB	replicative DNA helicase
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (166/171); minor base A (0/1); total (166/172)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 338 total aligned reads displayed.

AAGGAGGACTTCTTCCGGCATGAGCATCGGCTGATCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGNCTACCTCGGCCAGTTCCCCAACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCAGGACCGCCTCGAACCACCAGGCAAATCCCTCTGAGGTTCAG  >  NC_008463/5256229‑5256522

aaGGAGGACTTCTTCCGGCATGAGCATCGGCTGATCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTc                                                                                                                                                  >  2:6439957/1‑150 (MQ=255)

  ggaggaCTTCTTCCGGCATGAGCATCGGCTGATCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTcgc                                                                                                                                                >  2:394548/1‑148 (MQ=255)

   gaggaCTTCTTCCGGCATGAGCATCGGCTGATCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTcgcc                                                                                                                                               >  1:7504410/1‑150 (MQ=255)

    cggaCTTCTTCCGGCATGAGCATCGGCTGATCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTcgccg                                                                                                                                              <  2:8181694/149‑2 (MQ=255)

      gaCTTCTTCCGGCATGAGCATCGGCTGATCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCcgac                                                                                                                                            >  1:4847097/1‑150 (MQ=255)

       aCTTCTTCCGGCATGAGCATCGGCTGATCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCcgaca                                                                                                                                           <  1:6785474/150‑1 (MQ=255)

           cttcCGGCATGAGCATCGGCTGATCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAacac                                                                                                                                       <  1:883927/150‑1 (MQ=255)

            ttcCGGCATGAGCATCGGCTGATCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGTTGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACg                                                                                                                                      <  2:5793100/150‑1 (MQ=255)

               cGGCATGAGCATCGGCTGATCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGccc                                                                                                                                   <  2:6230164/150‑1 (MQ=255)

                 gCATGAGCATCGGCTGATCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTc                                                                                                                                 >  2:10197971/1‑150 (MQ=255)

                   aTGAGCATCGGCTGATCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCg                                                                                                                               <  2:4942978/150‑1 (MQ=255)

                   aTGAGCATCGGCTGATCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCg                                                                                                                               >  1:8931331/1‑150 (MQ=255)

                   aTGAGCATCGGCTGATCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCg                                                                                                                               >  2:3249386/1‑150 (MQ=255)

                    tGAGCATCGGCTGATCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGt                                                                                                                              >  2:1870442/1‑150 (MQ=255)

                     gAGCATCGGCTGATCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTg                                                                                                                             <  1:1449138/150‑1 (MQ=255)

                     gAGCATCGGCTGATCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTg                                                                                                                             <  1:694522/150‑1 (MQ=255)

                     gAGCATCGGCTGATCTGCACCGCCAGCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGCTGTCGTGACTGTGTCGGAAGCGAACGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTg                                                                                                                             <  2:10501735/150‑1 (MQ=255)

                      aGCATCGGCTGATCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTgg                                                                                                                            >  1:9557247/1‑150 (MQ=255)

                          tCGGCTGATCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCaa                                                                                                                        >  1:7841208/1‑150 (MQ=255)

                          tCGGCTGATCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCaa                                                                                                                        <  2:3034682/150‑1 (MQ=255)

                            ggCTGATCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAata                                                                                                                      <  2:5041102/150‑1 (MQ=255)

                            ggCTGATCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAata                                                                                                                      <  2:2175662/150‑1 (MQ=255)

                             gCTGATCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAatat                                                                                                                     <  2:1769627/150‑1 (MQ=255)

                             gCTGATCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAatat                                                                                                                     <  2:8682817/150‑1 (MQ=255)

                             gCTGATCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAatat                                                                                                                     <  1:1152311/150‑1 (MQ=255)

                              cTGATCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATc                                                                                                                    >  2:3566309/1‑150 (MQ=255)

                               tGATCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCg                                                                                                                   <  1:10584703/150‑1 (MQ=255)

                                 aTCTTCACCGCCATCAGCGATTTGGCCGCGCAGGATGCTCCGTTTGATGTCGTGACTGGGTCGCACGCGACCGAAGACCTTCCAGAAGCGGGCGGGCGGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATAACGAg                                                                                                                 <  2:4687378/150‑1 (MQ=255)

                                  tCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAgg                                                                                                                >  1:4145878/1‑150 (MQ=255)

                                   cTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCTTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGc                                                                                                               <  1:9370948/150‑1 (MQ=255)

                                     tCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCtt                                                                                                             <  2:698755/150‑1 (MQ=255)

                                      cACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTa                                                                                                            <  2:9375526/150‑1 (MQ=255)

                                         cGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTAcgc                                                                                                         <  2:2839013/150‑1 (MQ=255)

                                         cGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTAcgc                                                                                                         <  2:2263219/150‑1 (MQ=255)

                                          gCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTAcgcg                                                                                                        >  2:7283836/1‑150 (MQ=255)

                                           ccATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACgcgc                                                                                                       <  1:3751581/150‑1 (MQ=255)

                                              tCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGa                                                                                                    <  1:4519087/150‑1 (MQ=255)

                                              tCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGa                                                                                                    <  1:2235886/150‑1 (MQ=255)

                                               cAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGAt                                                                                                   <  2:4226102/150‑1 (MQ=255)

                                               cAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGAt                                                                                                   >  2:2419346/1‑150 (MQ=255)

                                               cAGCGAGGTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCGTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACACCACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGAt                                                                                                   >  1:573585/1‑150 (MQ=255)

                                                aGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATc                                                                                                  <  1:10024751/150‑1 (MQ=255)

                                                aGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATc                                                                                                  <  2:5593081/150‑1 (MQ=255)

                                                     gTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTcg                                                                                             >  2:6807803/1‑150 (MQ=255)

                                                     gTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTcg                                                                                             >  2:2886044/1‑150 (MQ=255)

                                                      ttGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTcgc                                                                                            <  2:9183443/150‑1 (MQ=255)

                                                        ggCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGa                                                                                          >  1:9458516/1‑150 (MQ=255)

                                                         gCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGAt                                                                                         >  2:9562903/1‑150 (MQ=255)

                                                         gCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGAt                                                                                         <  1:10312343/150‑1 (MQ=255)

                                                          ccGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATc                                                                                        <  2:6310448/150‑1 (MQ=255)

                                                           cgcgAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCg                                                                                       >  2:4339450/1‑150 (MQ=255)

                                                             cgAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCggg                                                                                     <  2:2306422/150‑1 (MQ=255)

                                                                  gATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCacac                                                                                <  2:9094862/150‑1 (MQ=255)

                                                                   aTGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACAcc                                                                               <  1:7975761/150‑1 (MQ=255)

                                                                    tGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCt                                                                              >  1:7709313/1‑150 (MQ=255)

                                                                     gCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTg                                                                             <  1:9966736/150‑1 (MQ=255)

                                                                     gCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTg                                                                             <  2:8460784/150‑1 (MQ=255)

                                                                      cTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGc                                                                            <  2:5434367/150‑1 (MQ=255)

                                                                        ccGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCgg                                                                          >  2:5979972/1‑150 (MQ=255)

                                                                         cGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGc                                                                         >  1:5431417/1‑150 (MQ=255)

                                                                            ttGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGc                                                                      >  1:9794286/1‑150 (MQ=255)

                                                                            ttGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGc                                                                      >  1:5242149/1‑150 (MQ=255)

                                                                             tGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCt                                                                     <  2:8405658/150‑1 (MQ=255)

                                                                              gATGTCGTGACTGTGTCGGAAGGGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGGCTGCTCGCCGACAACACGCGCTCCGTGGCCTCTTTCGAGGCTTAGGCGCAGAGCGTTCGCGATCGGGCTCCGCGGCGGCAGCTg                                                                    >  2:7777128/1‑150 (MQ=255)

                                                                              gATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTg                                                                    >  2:7467982/1‑150 (MQ=255)

                                                                                tGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGAt                                                                  <  1:701318/150‑1 (MQ=255)

                                                                                      gACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGtctct                                                            <  1:2298796/150‑1 (MQ=255)

                                                                                       aCTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGtctctc                                                           >  1:873662/1‑150 (MQ=255)

                                                                                           tgtCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGc                                                       >  2:1337302/1‑150 (MQ=255)

                                                                                               ggAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGcacca                                                   >  2:4602944/1‑150 (MQ=255)

                                                                                                   gCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGc                                               <  1:2710101/150‑1 (MQ=255)

                                                                                                     gATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCAc                                             >  1:3278192/1‑150 (MQ=255)

                                                                                                      aTCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCAcc                                            <  1:5101109/150‑1 (MQ=255)

                                                                                                         gAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCCgg                                         >  1:2122450/1‑150 (MQ=255)

                                                                                                           aGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCCGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCAGGAc                                       >  1:3591708/1‑150 (MQ=255)

                                                                                                           aGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCAGGAc                                       <  2:2083337/150‑1 (MQ=255)

                                                                                                              ccTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCAGGACCGc                                    >  1:6405911/1‑150 (MQ=255)

                                                                                                                 tCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCAGGACCGCCTc                                 <  2:7835099/150‑1 (MQ=255)

                                                                                                                    aGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCAGGACCGCCTCGaa                              >  2:8883290/1‑150 (MQ=255)

                                                                                                                     gAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCAGGACCGCCTCGAac                             >  2:3151198/1‑150 (MQ=255)

                                                                                                                       aGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCAGGACCGCCTCGAacca                           <  1:7268947/150‑1 (MQ=255)

                                                                                                                        gCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCAGGACCGCCTCGAaccac                          >  1:171143/1‑150 (MQ=255)

                                                                                                                        gCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCAGGACCGCCTCGAaccac                          >  1:10696518/1‑150 (MQ=255)

                                                                                                                        gCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCAGGACCGCCTCGAaccac                          >  2:7931057/1‑150 (MQ=255)

                                                                                                                          tGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCAGGACCGCCTCGAAccacca                        <  2:3442093/150‑1 (MQ=255)

                                                                                                                          tGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCAGGACCGCCTCGAAccacca                        >  1:1822898/1‑150 (MQ=255)

                                                                                                                            gCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCAGGACCGCCTCGAACCACCAgg                      >  2:1069952/1‑150 (MQ=255)

                                                                                                                            gCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCAGGACCGCCTCGAACCACCAgg                      >  2:7083942/1‑150 (MQ=255)

                                                                                                                            gCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCAGGACCGCCTCGAACCACCAgg                      <  1:5837728/150‑1 (MQ=255)

                                                                                                                               ggCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCAGGACCGCCTCGAACCACCAGGCaa                   >  1:2516230/1‑150 (MQ=255)

                                                                                                                                  tGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCAGGACCGCCTCGAACCACCAGGCAAATc                <  1:5013021/150‑1 (MQ=255)

                                                                                                                                   ggCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCAGGACCGCCTCGAACCACCAGGCAAATcc               <  2:9730899/150‑1 (MQ=255)

                                                                                                                                    gCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCAGGACCGCCTCGAACCACCAGGCAAATccc              >  1:4396212/1‑150 (MQ=255)

                                                                                                                                    gCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCAGGACCGCCTCGAACCACCAGGCAAATccc              >  2:4133395/1‑150 (MQ=255)

                                                                                                                                         ccTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCAGGACCGCCTCGAACCACCAGGCAAATCCCTCTGa         >  1:6238491/1‑150 (MQ=255)

                                                                                                                                            cGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCAGGACCGCCTCGAACCACCAGGCAAATCCCTCTGAGGt      >  2:2724549/1‑150 (MQ=255)

                                                                                                                                            cGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCAGGACCGCCTCGAACCACCAGGCAAATCCCTCTGAGGt      <  2:2014996/150‑1 (MQ=255)

                                                                                                                                               ccAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCAGGACCGCCTCGAACCACCAGGCAAATCCCTCTGAGGTTCa   <  2:4795225/150‑1 (MQ=255)

                                                                                                                                               ccAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCAGGACCGCCTCGAACCACCAGGCAAATCCCTCTGAGGTTCa   <  1:5686982/150‑1 (MQ=255)

                                                                                                                                                cAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCAGGACCGCCTCGAACCACCAGGCAAATCCCTCTGAGGTTCag  >  2:1511621/1‑150 (MQ=255)

AAGGAGGACTTCTTCCGGCATGAGCATCGGCTGATCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGNCTACCTCGGCCAGTTCCCCAACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCAGGACCGCCTCGAACCACCAGGCAAATCCCTCTGAGGTTCAG  >  NC_008463/5256229‑5256522

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`