Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_008463	732,660	G→A	intergenic (+114/‑426)	tyrS → / → PA14_RS03450	tyrosine‑‑tRNA ligase/16S ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_008463	732,660	0	G	A	100.0%	295.5 / NA	78	intergenic (+114/‑426)	tyrS/PA14_RS03450	tyrosine‑‑tRNA ligase/16S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (47/31);  total (47/31)

GGCTTTCGCGCGCATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGGGTGGTGAAGCGCTCCGGCAGGTTGTTCGCCGCAG  >  NC_008463/732513‑732694                                                                                                                                                    |                                   ggCTTTCGCGCGCATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGagt                                  <  2:3750508/150‑1 (MQ=255) ggCTTTCGCGCGCATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGagt                                  >  1:7917029/1‑150 (MQ=255) ggCTTTCGCGCGCATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGagt                                  <  2:6548088/150‑1 (MQ=255)   cTTTCGCGCGCATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGagtgg                                <  1:10003855/150‑1 (MQ=255)   cTTTCGCGCGCATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGagtgg                                >  1:4096508/1‑150 (MQ=255)   cTTTCGCGCGCATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGagtgg                                <  1:9424991/150‑1 (MQ=255)   cTTTCGCGCGCATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGagtgg                                <  2:4375567/150‑1 (MQ=255)   cTTTCGCGCGCATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGagtgg                                >  1:2435035/1‑150 (MQ=255)   cTTTCGCGCGCATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGagtgg                                >  1:2434879/1‑150 (MQ=255)    tttCGCGCGCATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGagtggt                               >  2:4547458/1‑150 (MQ=255)    tttCGCGCGCATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGACAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGAGTCTCTCCCAGGagtggt                               <  2:5553611/150‑1 (MQ=255)    tgtCGCGCGCATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGagtggt                               <  2:5553745/148‑1 (MQ=255)     ttCGCGCGCATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAgtggtg                              <  1:10118219/150‑1 (MQ=255)     ttCGCGCGCATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAgtggtg                              <  1:5582699/150‑1 (MQ=255)     ttCGCGCGCATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAgtggtg                              <  1:10118199/150‑1 (MQ=255)      tCGCGCGCATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGa                             <  1:6440838/150‑1 (MQ=255)      tCGCGCGCATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGa                             >  1:5184253/1‑150 (MQ=255)      tCGCGCGCATCACGCTGAAAGCTGAATAATTCGTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGa                             <  1:594636/150‑1 (MQ=255)       cgcgcgCATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGaa                            <  1:3991024/150‑1 (MQ=255)       cgcgcgCATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGaa                            <  1:7496980/150‑1 (MQ=255)       cgcgcgCATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGaa                            <  1:3993722/150‑1 (MQ=255)        gcgcgcATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAg                           >  2:8019752/1‑150 (MQ=255)         cgcgcATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATACTGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAgc                          <  2:3623036/150‑1 (MQ=255)         cgcgcATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAgc                          >  1:3963870/1‑150 (MQ=255)         cgcgcATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAgc                          >  1:3959113/1‑150 (MQ=255)         cgcgcATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAgc                          <  2:3623035/150‑1 (MQ=255)         cgcgcATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAgc                          <  2:3628018/150‑1 (MQ=255)          gcgcATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAgcg                         <  1:8964753/150‑1 (MQ=255)          gcgcATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAgcg                         >  1:3397226/1‑150 (MQ=255)           cgcATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAgcgc                        >  1:7497402/1‑150 (MQ=255)           cgcATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAgcgc                        <  2:6246566/150‑1 (MQ=255)            gcATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCt                       >  1:5854723/1‑150 (MQ=255)            gcATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCt                       >  1:6266180/1‑150 (MQ=255)            gcATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCt                       <  1:6286583/150‑1 (MQ=255)             cATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTc                      <  1:544368/150‑1 (MQ=255)             cATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTc                      <  1:6042166/150‑1 (MQ=255)             cATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTc                      >  1:1967944/1‑150 (MQ=255)             cATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTc                      <  2:7670533/150‑1 (MQ=255)             cATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTc                      >  1:7562027/1‑150 (MQ=255)              aTCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTcc                     <  1:7679491/150‑1 (MQ=255)              aTCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTcc                     <  1:7673353/150‑1 (MQ=255)              aTCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTcc                     >  1:8817825/1‑150 (MQ=255)              aTCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTcc                     <  1:10084743/150‑1 (MQ=255)              aTCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTcc                     <  1:10096777/150‑1 (MQ=255)              aTCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTcc                     <  1:3251240/150‑1 (MQ=255)              aTCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTcc                     >  1:2137097/1‑150 (MQ=255)                cACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCgg                   >  2:10014312/1‑150 (MQ=255)                 aCGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGc                  >  1:4779364/1‑150 (MQ=255)                 aCGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATACTCTCCCAGGAGTGGTGAAGCGCTCCGGc                  >  2:3248837/1‑150 (MQ=255)                   gCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAg                >  1:9392021/1‑150 (MQ=255)                   gCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAg                >  2:7544788/1‑150 (MQ=255)                    cTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAgg               >  2:3181344/1‑150 (MQ=255)                     tGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGTAGCGCTCCGGCAggg              >  2:5574569/1‑149 (MQ=255)                     tGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGgt              >  1:2089435/1‑150 (MQ=255)                     tGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGgt              >  1:3638257/1‑150 (MQ=255)                      gAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGgtt             >  2:6381202/1‑150 (MQ=255)                      gAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGgtt             >  1:2179077/1‑150 (MQ=255)                      gAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGgtt             >  1:2179114/1‑150 (MQ=255)                      gAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGgtt             <  2:4278278/150‑1 (MQ=255)                       aaaGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGgttg            >  1:5345963/1‑150 (MQ=255)                        aaGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGgttgt           <  2:6077073/150‑1 (MQ=38)                        aaGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGgttgt           >  2:8540846/1‑150 (MQ=38)                        aaGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGgttgt           >  2:8540819/1‑150 (MQ=38)                        aaGCTGAAGAATTCTTTCAACTAAGTGTTGACGCCAGAGTCAATCTCCCTATAATGCGCACCCCTCCCGGCGACGACGACGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGgttgt           <  2:6077217/150‑1 (MQ=11)                         aGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGgttgtt          >  1:1089145/1‑150 (MQ=38)                         aGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGgttgtt          <  2:5366390/150‑1 (MQ=38)                         aGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTAGAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGgttgtt          >  2:1853358/1‑150 (MQ=255)                          gCTGAATAATTCTTTCAACTAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGGTTGTTc         <  2:6487232/150‑1 (MQ=255)                          gCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGGTTGTTc         >  2:3112871/1‑150 (MQ=37)                          gCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGGTTGTTc         >  2:301672/1‑150 (MQ=37)                          gCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGGTTGTTc         >  2:4312748/1‑150 (MQ=37)                            tGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCTGGTTGTTcgc       >  2:5614569/1‑150 (MQ=25)                            tGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGGTTGTTcgc       >  1:370073/1‑150 (MQ=25)                            tGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGGTTGTTcgc       >  1:8392005/1‑150 (MQ=25)                            tGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGGTTGTTcgc       >  1:4328529/1‑150 (MQ=25)                            tGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGGTTGTTcgc       >  2:1061570/1‑150 (MQ=25)                             gAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGGTTGTTcgcc      <  1:41504/150‑1 (MQ=21)                              aataatTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGGTTGTTcgccg     >  2:5241199/1‑150 (MQ=18)                              aataatTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGGTTGTTcgccg     >  1:3020945/1‑150 (MQ=18)                              aataatTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGGTTGTTcgccg     >  1:3020946/1‑150 (MQ=18)                              aataatTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGGTTGTTcgccg     >  1:6250777/1‑150 (MQ=18)                              aataatTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGGTTGTTcgccg     >  1:6250957/1‑150 (MQ=18)                               ataatTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGGTTGTTcgccgc    >  1:8201679/1‑150 (MQ=17)                                taatTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGGTTGTTCGCCGCa   <  2:4876041/150‑1 (MQ=17)                                taatTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGGTTGTTCGCCGCa   <  1:7413742/150‑1 (MQ=17)                                 aatTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGGTTGTTCGCCGCAg  <  2:2213926/150‑1 (MQ=14)                                 aatTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGGTTGTTCGCCGCAg  >  2:2250134/1‑150 (MQ=14)                                 aatTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGGTTGTTCGCCGCAg  >  1:6245283/1‑150 (MQ=14)                                 aatTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGGTTGTTCGCCGCAg  >  2:7397352/1‑150 (MQ=14)                                 aatTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGGTTGTTCGCCGCAg  >  1:7586656/1‑150 (MQ=14)                                                                                                                                                    |                                   GGCTTTCGCGCGCATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGGGTGGTGAAGCGCTCCGGCAGGTTGTTCGCCGCAG  >  NC_008463/732513‑732694

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑