BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_008463	5,809,315	G→C	intergenic (‑201/+18)	rpsF ← / ← rlmB	30S ribosomal protein S6/23S rRNA (guanosine(2251)‑2'‑O)‑methyltransferase RlmB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_008463	5,809,315	0	G	C	100.0%	1485.4 / NA	386	intergenic (‑201/+18)	rpsF/rlmB	30S ribosomal protein S6/23S rRNA (guanosine(2251)‑2'‑O)‑methyltransferase RlmB
Reads supporting (aligned to +/- strand): ref base G (0/0); new base C (210/176); total (210/176)

Only 100 of 386 total aligned reads displayed.

CTTGGCTTGCCTGGTGGAGAGGCGAACGAACGCCTGCCCGCGCGGCAAGGGGCGACATTCTAGAGAAGGGCAACCGGCGAAGCAAGGAAGATTGGCGAATATTTGTACAGCGGATGGAATGGGCGCGCGGGGCCCCCCCCGGGGGGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTTGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAG  >  NC_008463/5809174‑5809461

cgcggCGTGCCGGGTGGCGCGCCGAACGAACGCCGGCCCGCGCGGCAAGGGGCGACATTCTAGACCAGGGCACCCGGCGAACCAAGGAAGATTGGCGAATATTTGTACAGCGGATGGAATG‑‑‑GCGCGGGG‑CCGCGCCGCGGCGGCCCCGCa                                                                                                                                        <  1:5415807/147‑1 (MQ=255)

  tcttggcttgcctggtggagaggcgaacgaacgcctgcccgcgcggcaaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGc                                                                                                                                         >  2:478576‑M2/124‑150 (MQ=255)

   cttggcttgcctggtggagaggcgaacgaacgcctgcccgcgcggcaaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCa                                                                                                                                        >  1:10532418‑M2/123‑150 (MQ=255)

     tggcttgcctggtggagaggcgaacgaacgcctgcccgcgcggcaaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCAcg                                                                                                                                      >  2:3527584‑M2/121‑150 (MQ=255)

      ggcttgcctggtggagaggcgaacgaacgcctgcccgcgcggcaaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCAcgc                                                                                                                                     >  2:768109‑M2/120‑150 (MQ=255)

        cttgcctggtggagaggcgaacgaacgcctgcccgcgcggcaaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACgcgc                                                                                                                                   >  2:4061590‑M2/118‑150 (MQ=255)

           gcctggtggagaggcgaacgaacgcctgcccgcgcggcaaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCa                                                                                                                                >  2:2376451‑M2/115‑150 (MQ=255)

                      aggcgaacgaacgcctgcccgcgcggcaaggggcgacattctagagaagggcaaccggcgaagccaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCgggg                                                                                                                     >  2:2238471‑M2/104‑150 (MQ=255)

                       ggcgaacgaacgcctgcccgcgcggcaaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtaccgcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGt                                                                                                                    <  2:7569964‑M2/48‑1 (MQ=255)

                       ggcgaacgaacgcctgcccgcgcggcaaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGt                                                                                                                    >  2:5690711‑M2/103‑150 (MQ=255)

                       ggcgaacgaacgcctgcccgcgcggcaaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGt                                                                                                                    >  2:6723141‑M2/103‑150 (MQ=255)

                        gcgaacgaacgcctgcccgcgcggcaaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTg                                                                                                                   <  1:5999560‑M2/49‑1 (MQ=255)

                         cgaacgaacgcctgcccgcgcggcaaggggcgacattctagagaagggcaaccggcgaagcacggaagattggcgactatgtgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCCGCGGGGTgc                                                                                                                  >  2:5435736‑M2/101‑150 (MQ=255)

                          gaacgaacgcctgcccgcgcggcaaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTgcg                                                                                                                 >  1:8176766‑M2/100‑150 (MQ=255)

                          gaacgaacgcctgcccgcgcggcaaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTgcg                                                                                                                 >  2:8478673‑M2/100‑150 (MQ=255)

                           aacgaacgcctgcccgcgcggcaaggggcgaccttctagacacgggcaaccggcgaagcaaggaagattggcgaatatttgtaccgcggatggaagggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTgcgc                                                                                                                <  2:3338826‑M2/52‑1 (MQ=255)

                           aacgaacgcctgcccgcgcggcaaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTgcgc                                                                                                                >  1:7984307‑M2/99‑150 (MQ=255)

                           aacgaacgcctgcccgcgcggcaaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTgcgc                                                                                                                >  2:9793579‑M2/99‑150 (MQ=255)

                             cgaacgcctgcccgcgcggcaaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTg                                                                                                              >  1:5223835‑M2/97‑150 (MQ=255)

                               aacgccggcccgcgcggcaaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtaccgcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGc                                                                                                            <  1:4658876‑M2/56‑1 (MQ=255)

                                 cgcctgcccgcgcggcaaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCgg                                                                                                          <  1:1240434‑M2/58‑1 (MQ=255)

                                  gcctgcccgcgcggcaaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCgga                                                                                                         <  1:8648834‑M2/59‑1 (MQ=255)

                                     tgcccgcgcggcaaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCCCGCGCTCACTCCAGCGGGGGGGGCTGGGggagga                                                                                                      >  1:49149‑M2/89‑150 (MQ=255)

                                     tgcccgcgcggcaaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCggagga                                                                                                      >  2:2603609‑M2/89‑150 (MQ=255)

                                         cgcgcggcaaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggagggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCCCGCGCTCACTCCCGCGGGGTGCGCTGGCGGCGGATctc                                                                                                  >  2:5634059‑M2/85‑149 (MQ=255)

                                            gcggcaaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgactatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCAAGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGaa                                                                                               <  2:2678268‑M2/69‑1 (MQ=255)

                                               gcaaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCCCACCCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAg                                                                                            >  1:8946809‑M2/79‑150 (MQ=255)

                                                  aggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCa                                                                                         >  1:2534518‑M2/76‑150 (MQ=255)

                                                  aggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCa                                                                                         >  1:2941711‑M2/76‑150 (MQ=255)

                                                     ggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggTGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGAc                                                                                      <  2:7515308‑M2/78‑1 (MQ=255)

                                                     ggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGAc                                                                                      >  2:4439544‑M2/73‑150 (MQ=255)

                                                      gcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACg                                                                                     >  2:143161‑M2/72‑150 (MQ=255)

                                                       cgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGc                                                                                    <  1:10650158‑M2/80‑1 (MQ=255)

                                                         acattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCg                                                                                  >  1:8961414‑M2/69‑150 (MQ=255)

                                                          cattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCgg                                                                                 >  2:3102620‑M2/68‑150 (MQ=255)

                                                           attctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGt                                                                                <  2:2518404‑M2/84‑1 (MQ=255)

                                                            ttctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTg                                                                               <  1:4144848‑M2/85‑1 (MQ=255)

                                                             tctagagaagggcaaccggcgaagcaaggaagattggcgattatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCTGCCCCGCACGCGCTCAGTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTgg                                                                              <  2:6545974‑M2/86‑1 (MQ=255)

                                                               tagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCg                                                                            <  1:2369306‑M2/88‑1 (MQ=255)

                                                               tagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCg                                                                            <  1:6524310‑M2/88‑1 (MQ=255)

                                                                 gagaagggcaaccggcgaagcaaggaagattggcgaatatttgtccagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGAc                                                                          <  2:4477237‑M2/90‑1 (MQ=255)

                                                                 gagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggactggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGAc                                                                          >  2:731569‑M2/61‑150 (MQ=255)

                                                                 gagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGAc                                                                          >  1:10160782‑M2/61‑150 (MQ=255)

                                                                   gaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCg                                                                        >  2:8046000‑M2/59‑150 (MQ=255)

                                                                     agggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCGCGAACAGGCAGACGCCGGTGGCGAccgcg                                                                      >  1:3571344‑M2/57‑148 (MQ=255)

                                                                      gggcaaccggcgaagcaaggacgattggcgaatatttgtccagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCgaga                                                                     <  1:10014297‑M2/95‑1 (MQ=255)

                                                                       ggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGAc                                                                    <  2:2842864‑M2/96‑1 (MQ=255)

                                                                           accggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTc                                                                >  1:9670593‑M2/51‑150 (MQ=255)

                                                                           accggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTc                                                                >  2:8693594‑M2/51‑150 (MQ=255)

                                                                             cggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAg                                                              <  2:1676649‑M2/102‑1 (MQ=255)

                                                                              ggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAgg                                                             >  2:6146502‑M2/48‑150 (MQ=255)

                                                                               gcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGCTCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGgc                                                            >  1:6749973‑M2/47‑150 (MQ=255)

                                                                               gcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCGCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGgc                                                            >  1:6401966‑M2/47‑150 (MQ=255)

                                                                                cgaagcaaggaagattggcgaatatttcaacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGCGGAACTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGgct                                                           <  1:848827‑M2/105‑1 (MQ=255)

                                                                                  aagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGgctgc                                                         >  2:1837545‑M2/44‑150 (MQ=255)

                                                                                  aagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACACCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGgctgc                                                         >  1:9397996‑M2/44‑150 (MQ=255)

                                                                                   agcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGgctgct                                                        <  1:7511011‑M2/108‑1 (MQ=255)

                                                                                      aaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGAc                                                     >  2:5144127‑M2/40‑150 (MQ=255)

                                                                                      aaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACAGAGACGTTCAGGCTGCTGAc                                                     >  1:5882089‑M2/40‑150 (MQ=255)

                                                                                       aggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCTGTGGCGACCGAGACGTTCAGGCTGCTGACg                                                    >  2:10475955‑M2/39‑150 (MQ=255)

                                                                                       aggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACg                                                    <  2:5815526‑M2/112‑1 (MQ=255)

                                                                                         gaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCt                                                  <  1:8407487‑M2/114‑1 (MQ=255)

                                                                                         gaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCt                                                  >  1:1521222‑M2/37‑150 (MQ=255)

                                                                                          aagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTg                                                 >  2:429673‑M2/36‑150 (MQ=255)

                                                                                          aagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTg                                                 <  2:10050424‑M2/115‑1 (MQ=255)

                                                                                           agatgggcgaagatttgtaccgcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCGCAGTCCAGCGGGGTGCGCTGGCGGAGGATCTCGACCAGGCAGCCGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGc                                                <  2:6264657‑M2/116‑1 (MQ=255)

                                                                                              ttggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCgg                                             <  1:10353224‑M2/119‑1 (MQ=255)

                                                                                               tggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGc                                            <  1:733555‑M2/120‑1 (MQ=255)

                                                                                               tggcgaatatttggacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGCCCGAGACGATCAGGCTGCTGACGCTGCCGGc                                            <  2:8968011‑M2/120‑1 (MQ=255)

                                                                                                   gaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATc                                        <  1:2679275‑M2/124‑1 (MQ=255)

                                                                                                    aatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCCTCg                                       >  2:9068037‑M2/26‑150 (MQ=255)

                                                                                                    aatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCg                                       <  2:8787270‑M2/125‑1 (MQ=255)

                                                                                                     atatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCgg                                      <  2:7087552‑M2/126‑1 (MQ=255)

                                                                                                     atatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTAACTCCAGCGGGGTGCGCTGGCGGAGGAACTCGAACAGGCAGACCCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGATGCCGGCCatccg                                      >  1:7003966‑M2/25‑148 (MQ=255)

                                                                                                      tatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGc                                     >  2:9866711‑M2/24‑150 (MQ=255)

                                                                                                         ttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAAc                                  >  2:2081531‑M2/21‑150 (MQ=255)

                                                                                                            tacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTg                               <  1:6239424‑M2/133‑1 (MQ=255)

                                                                                                             acagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTgg                              >  2:6856330‑M2/17‑150 (MQ=255)

                                                                                                              cagcggatggactggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGc                             <  1:7861263‑M2/135‑1 (MQ=255)

                                                                                                                gcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCAGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATAGGCAACCTGGCGa                           >  1:3314977‑M2/14‑150 (MQ=255)

                                                                                                                  ggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAgg                         <  1:7652000‑M2/139‑1 (MQ=255)

                                                                                                                  ggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAgg                         >  2:8284435‑M2/12‑150 (MQ=255)

                                                                                                                    atggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGaa                       >  2:301602‑M2/10‑150 (MQ=255)

                                                                                                                    atggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGaa                       >  2:2390238‑M2/10‑150 (MQ=255)

                                                                                                                    agggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGaa                       <  2:1595562‑M2/141‑1 (MQ=255)

                                                                                                                      ggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGt                     >  2:8894902‑M2/8‑150 (MQ=255)

                                                                                                                        aatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGCCCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCg                   >  1:2080193‑M2/6‑150 (MQ=255)

                                                                                                                         atggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGACGTCGc                  >  1:3153761‑M2/5‑150 (MQ=255)

                                                                                                                          tggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCgcg                 >  1:189877‑M2/4‑149 (MQ=255)

                                                                                                                          tggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCa                 <  1:1163916‑M2/147‑1 (MQ=255)

                                                                                                                           ggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAg                <  1:9814919‑M2/148‑1 (MQ=255)

                                                                                                                           ggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAg                <  2:4865208‑M2/148‑1 (MQ=255)

                                                                                                                           ggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGGGCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAg                >  2:8624327‑M2/3‑150 (MQ=255)

                                                                                                                                   ggccgcgccGCGGCGGCCCCGCACGCGCTCCCTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTcgc         <  2:127037/143‑1 (MQ=255)

                                                                                                                                    gccgcgccGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGTCGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTcgcg        >  1:1839104/7‑150 (MQ=255)

                                                                                                                                      cgcgccGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCggg      <  2:5868173/146‑1 (MQ=255)

                                                                                                                                        cgccGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACTGGCAGACGCCGGTGGCGTCCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCCACCTGGCGCGGAAGTCGCAGTGCTCGCGGGTc    >  1:4501608/3‑150 (MQ=255)

                                                                                                                                        cgccGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTc    <  1:2155922/148‑1 (MQ=255)

                                                                                                                                         gccGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCa   >  2:10208406/2‑150 (MQ=255)

                                                                                                                                          ccGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAg  >  1:6705864/1‑150 (MQ=255)

CTTGGCTTGCCTGGTGGAGAGGCGAACGAACGCCTGCCCGCGCGGCAAGGGGCGACATTCTAGAGAAGGGCAACCGGCGAAGCAAGGAAGATTGGCGAATATTTGTACAGCGGATGGAATGGGCGCGCGGGGCCCCCCCCGGGGGGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTTGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAG  >  NC_008463/5809174‑5809461

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`