Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 5,866,730 T→G A733A (GCA→GCC)  PA14_RS26890 ← ATP‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084635,866,7300TG99.8% 2094.5 / NA 540A733A (GCA→GCC) PA14_RS26890ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base G (325/214);  total (325/215)
Fisher's exact test for biased strand distribution p-value = 3.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 540 total aligned reads displayed.

GGATGAGCGGATTATTGGCGGCGGATGTAAGACGCCGTTGAACGCGCGGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCTGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAGCGCCACCAGGCCCATGGAGTGGCAGCTTCCGGACAGGCGATG  >  NC_008463/5866583‑5866876
                                                                                                                                                   |                                                                                                                                                  
 gATGAGCGGATTATTGGCGGCGGATGTAAGACGCCGTTGAACGCGCGGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGgcg                                                                                                                                                 >  2:1756026/1‑150 (MQ=255)
   tGAGCGGATTATTGGCGGCGGATGTAAGACGCCGTTGAACGCGCGGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGcc                                                                                                                                               >  2:5919826/1‑150 (MQ=255)
   tGAGCGGATTATTGGCGGCGGATGTAAGACGCCGTTGAACGCGCGGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGcc                                                                                                                                               <  1:5616634/150‑1 (MQ=255)
    gAGCGGATTATTGGCGGCGGATGTAAGACGCCGTTGAACGCGCGGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCg                                                                                                                                              >  2:7676316/1‑150 (MQ=255)
    gAGCGGATTATTGGCGGCGGATGTAAGACGCCGTTGAACGCGCGGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCg                                                                                                                                              <  2:392165/150‑1 (MQ=255)
      gCGGATTATTGGCGGCGGATGTAAGACGCCGTTGAACGCGCGGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTc                                                                                                                                            >  1:10612765/1‑150 (MQ=255)
       cGGATTATTGGCGGCGGATGTAAGACGCCGTTGAACGCGCGGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTcg                                                                                                                                           <  1:3767264/150‑1 (MQ=255)
        ggATTATTGGCGGCGGATGTAAGACGCCGTTGAACGCGCGGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTcgc                                                                                                                                          >  1:5384104/1‑150 (MQ=255)
          attattGGCGGCGGATGTAAGACGCCGTTGAACGCGCGGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCgcgg                                                                                                                                        <  1:7148076/150‑1 (MQ=255)
            tattGGCGGCGGATGTAAGACGCCGTTGAACGCGCGGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCgcggcg                                                                                                                                      >  1:6113989/1‑150 (MQ=255)
               tGGCGGCGGATGTAAGACGCCGTTGAACGCGCGGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAg                                                                                                                                   <  2:10181468/150‑1 (MQ=255)
                   ggcggATGTAAGACGCCGTTGAACGCGCGGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCtc                                                                                                                               >  2:7297197/1‑149 (MQ=255)
                   ggcggATGTAAGACGCCGTTGAACGCGCGGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCCCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTa                                                                                                                               >  1:950099/1‑150 (MQ=255)
                    gcggATGTAAGACGCCGTTGAACGCGCGGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTAc                                                                                                                              <  1:4775282/150‑1 (MQ=255)
                      ggATGTAAGACGCCGTTGAACGCGCGGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTAccc                                                                                                                            >  2:8971928/1‑150 (MQ=255)
                       gATGTAAGACGCCGTTGAACGCGCGGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCa                                                                                                                           >  1:5778730/1‑150 (MQ=255)
                           tAAGACGCCGTTGAACGCGCGGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGACCCGTCGCGCCGCAGGCTCCCCAgcag                                                                                                                       >  2:9794001/1‑147 (MQ=255)
                            aaGACGCCGTTGAACGCGCGGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGt                                                                                                                      >  1:4207053/1‑150 (MQ=255)
                              gACGCCGTTGAACGCGCGGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTgc                                                                                                                    >  1:5513899/1‑150 (MQ=255)
                                  ccGTTGAACGCGCGGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGccc                                                                                                                >  2:6522835/1‑150 (MQ=255)
                                  ccGTTGAACGCGCGGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGccc                                                                                                                >  2:1159405/1‑150 (MQ=255)
                                   cGTTGAACGCGCGGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGcccc                                                                                                               <  1:305897/150‑1 (MQ=255)
                                   cGTTGAACGCGCGGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGcccc                                                                                                               >  2:809062/1‑150 (MQ=255)
                                       gAACGCGCGGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGc                                                                                                           <  2:4824587/150‑1 (MQ=255)
                                       gAACGCGAGGATCGAAGATGTAAAAAGCTGTAAGGAAGTGGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGc                                                                                                           <  2:5504998/150‑1 (MQ=255)
                                           gcgcgGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTcgc                                                                                                       >  1:4062098/1‑150 (MQ=255)
                                            cgcgGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTcgcg                                                                                                      >  2:3316337/1‑150 (MQ=255)
                                             gcgGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCgg                                                                                                     >  1:9572830/1‑150 (MQ=255)
                                             gcgGAGCGAAGATGTAAAAAGCTGTACGGAAGTAGGTCGCCGCGCCGCTCAACCGGCCGCTGACTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCGTCGGCGCCGACGCGGCGCAGGCTTCCCAGGCGTGCGCCCCAGGCTCGCgg                                                                                                     <  2:5323778/150‑1 (MQ=255)
                                               ggATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGt                                                                                                   >  1:9409238/1‑150 (MQ=255)
                                               ggATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGt                                                                                                   <  2:9672293/150‑1 (MQ=255)
                                                   cGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGAc                                                                                               >  2:3433156/1‑150 (MQ=255)
                                                   cGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGAc                                                                                               >  1:3732099/1‑150 (MQ=255)
                                                    gAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGAcc                                                                                              >  2:5669915/1‑150 (MQ=255)
                                                     aaGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGAccc                                                                                             >  2:10396185/1‑150 (MQ=255)
                                                     aaGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGAccc                                                                                             >  2:615159/1‑150 (MQ=255)
                                                        aTGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCgg                                                                                          >  1:740607/1‑150 (MQ=255)
                                                         tGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGc                                                                                         <  1:2481118/150‑1 (MQ=255)
                                                         tGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGc                                                                                         <  1:6766440/150‑1 (MQ=255)
                                                         tGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGc                                                                                         >  2:1972825/1‑150 (MQ=255)
                                                          gTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGcc                                                                                        >  2:4978861/1‑150 (MQ=255)
                                                          gTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGcc                                                                                        >  1:8280282/1‑150 (MQ=255)
                                                             aaaaGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCcgcg                                                                                     >  1:5067058/1‑150 (MQ=255)
                                                              aaaGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCgcgc                                                                                    >  1:8086841/1‑150 (MQ=255)
                                                                  cTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAg                                                                                <  1:2775496/150‑1 (MQ=255)
                                                                    gTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAggg                                                                              >  2:2101817/1‑150 (MQ=255)
                                                                       aGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCt                                                                           >  1:8947736/1‑150 (MQ=255)
                                                                       aGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCt                                                                           >  1:9982099/1‑150 (MQ=255)
                                                                         gAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTcg                                                                         >  1:10025289/1‑150 (MQ=255)
                                                                           aGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTcgcg                                                                       >  2:10470271/1‑150 (MQ=255)
                                                                            gttgttCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCgcgc                                                                      >  2:10693403/1‑150 (MQ=255)
                                                                              tgttCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGAAGGGCTACGGCGCCGGCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCACGCGCTc                                                                    >  2:3243306/1‑150 (MQ=255)
                                                                               gttCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCGCGCGCGcc                                                                   >  2:9186241/1‑150 (MQ=255)
                                                                                   gccgcgccgcTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGGGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCt                                                               <  1:220158/150‑1 (MQ=255)
                                                                                    ccgcgccgcTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTc                                                              >  2:4655570/1‑150 (MQ=255)
                                                                                      gcgccgcTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGc                                                            >  1:10224355/1‑150 (MQ=255)
                                                                                         ccgcTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCa                                                         >  1:65786/1‑150 (MQ=255)
                                                                                         ccgcTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCa                                                         >  2:1278289/1‑150 (MQ=255)
                                                                                          cgcTCAACCGGCCGCTGAGTCGGCGTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACGCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAg                                                        <  2:81718/150‑1 (MQ=255)
                                                                                           gcTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAgg                                                       >  2:622085/1‑150 (MQ=255)
                                                                                            cTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGc                                                      >  2:1611965/1‑150 (MQ=255)
                                                                                            cTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGc                                                      >  1:9265896/1‑150 (MQ=255)
                                                                                             acAACCGGCCGCTTAGTCGGCTGCGACGTGGAATCCGGCGCGGCGCAGTGAGTCGGCGCCTTCGAGGAGCAGGCTACCAAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCATGCCGAGACCTGGTCCTCGCCCTCCAGCTCGCCCAGGcc                                                     <  2:5149742/149‑1 (MQ=255)
                                                                                              cAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCg                                                    >  1:3831529/1‑150 (MQ=255)
                                                                                               aaCCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCgg                                                   >  2:9475177/1‑150 (MQ=255)
                                                                                                 ccGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGgcg                                                 >  2:7874678/1‑150 (MQ=255)
                                                                                                 ccGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGgcg                                                 <  1:2660089/150‑1 (MQ=255)
                                                                                                 ccGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGgcg                                                 >  1:1548985/1‑150 (MQ=255)
                                                                                                   ggCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGcc                                               >  1:1245466/1‑150 (MQ=255)
                                                                                                    gCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCg                                              <  1:8800031/150‑1 (MQ=255)
                                                                                                           aGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAGCGcca                                       >  2:6636596/1‑150 (MQ=255)
                                                                                                             tCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAGCGccacc                                     <  1:1698390/150‑1 (MQ=255)
                                                                                                               ggCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAGCGCCACCAg                                   >  2:255206/1‑150 (MQ=255)
                                                                                                               ggCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAGCGCCACCAg                                   <  1:5930069/150‑1 (MQ=255)
                                                                                                                gCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAGCGCCACCAgg                                  <  1:4012545/150‑1 (MQ=255)
                                                                                                                gCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCGCGCGCTCCAGCGCGCCCAGGCCGGAGCCGAGCGCCACCAgg                                  >  2:7437463/1‑150 (MQ=255)
                                                                                                                   tCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAGCGCCACCAGGccc                               <  1:4380659/150‑1 (MQ=255)
                                                                                                                   tCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAGCGCCACCAGGccc                               >  1:3282275/1‑150 (MQ=255)
                                                                                                                       cGAGGAAGCCGGCGCGGCGCCGCGCGTCGGCGCCGTCGCGGCGCAGGCGACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAGCGCCACCAGGCCCATgg                           <  2:8804735/150‑1 (MQ=255)
                                                                                                                       cGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAGCGCCACCAGGCCCATgg                           <  2:3085582/150‑1 (MQ=255)
                                                                                                                         aGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAGCGCCACCAGGCCCATGGAg                         <  2:4041054/150‑1 (MQ=255)
                                                                                                                           gAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAGCGCCACCAGGCCCATGGAGTg                       <  1:7768327/150‑1 (MQ=255)
                                                                                                                              gCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAGCGCCACCAGGCCCATGGAGTGGCa                    <  2:8559126/150‑1 (MQ=255)
                                                                                                                                cggcgcggcgCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAGCGCCACCAGGCCCATGGAGTGGCAGc                  <  1:9840603/150‑1 (MQ=255)
                                                                                                                                 ggcgcggcgcAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAGCGCCACCAGGCCCATGGAGTGGCAGCt                 >  1:2816819/1‑150 (MQ=255)
                                                                                                                                 ggcgcggcgcAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAGCGCCACCAGGCCCATGGAGTGGCAGCt                 >  2:699151/1‑150 (MQ=255)
                                                                                                                                  gcgcggcgcAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAGCGCCACCAGGCCCATGGAGTGGCAGCtt                <  1:1765408/150‑1 (MQ=255)
                                                                                                                                   cgcggcgcAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAGCGCCACCAGGCCCATGGAGTGGCAGCTTc               <  1:8460133/150‑1 (MQ=255)
                                                                                                                                   cgcggcgcAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAGCGCCACCAGGCCCATGGAGTGGCAGCTTc               <  2:2367381/150‑1 (MQ=255)
                                                                                                                                   cgcggcgcAGGGCTTCGGCGCCGTCGCGGCGCAGGCGACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAGCGCCACCAGGCCCATGGAGTGGCAGCTTc               <  2:8321798/150‑1 (MQ=255)
                                                                                                                                      ggcgcAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAGCGCCACCAGGCCCATGGAGTGGCAGCTTCCgg            <  1:4586706/150‑1 (MQ=255)
                                                                                                                                       gcgcAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAGCGCCACCAGGCCCATGGAGTGGCAGCTTCCGGa           >  1:6834439/1‑150 (MQ=255)
                                                                                                                                        cgcAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGCCCGCGCCCAGCGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAGCGCCACCAGGCCCATGGAGTGGCAGCTTCCGGAc          <  2:10606354/150‑1 (MQ=255)
                                                                                                                                         gcAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAGCGCCACCAGGCCCATGGAGTGGCAGCTTCCGGACa         >  1:7588005/1‑150 (MQ=255)
                                                                                                                                          cAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGTGCCTCGCGCTACAGCTCGCCCTGGCCGGAGCCGAGCGCCACCAGGCCCATGGAGTGGCAGCTTCCGGACAg        <  2:3596793/150‑1 (MQ=255)
                                                                                                                                            gggCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAGCGCCACCAGGCCCATGGAGTGGCAGCTTCCGGACAggc      <  1:10437555/150‑1 (MQ=255)
                                                                                                                                            gggCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAGCGCCACCAGGCCCATGGAGTGGCAGCTTCCGGACAggc      >  2:4210072/1‑150 (MQ=255)
                                                                                                                                             ggCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAGCGCCACCAGGCCCATGGAGTGGCAGCTTCCGGACAggcg     <  2:1493366/150‑1 (MQ=255)
                                                                                                                                              gCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAGCGCCACCAGGCCCATGGAGTGGCAGCTTCCGGACAggcga    >  1:8613322/1‑150 (MQ=255)
                                                                                                                                                ttCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAGCGCCACCAGGCCCATGGAGTGGCAGCTTCCGGACAggcgatg  >  2:2900466/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GGATGAGCGGATTATTGGCGGCGGATGTAAGACGCCGTTGAACGCGCGGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCTGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAGCGCCACCAGGCCCATGGAGTGGCAGCTTCCGGACAGGCGATG  >  NC_008463/5866583‑5866876

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑