Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 757,030 A→G T456A (ACC→GCC)  fusA → elongation factor G

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_008463757,0300AG89.3% 1205.8 / 136.3 393T456A (ACC→GCC) fusAelongation factor G
Reads supporting (aligned to +/- strand):  ref base A (19/23);  new base G (181/170);  total (200/193)
Fisher's exact test for biased strand distribution p-value = 5.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 393 total aligned reads displayed.

CGAGCGCATGGACTTCCCCGAGCCGGTAATCTCGGTCGCTGTAGAGCCGAAGACCAAGGCTGACCAGGAGAAGATGGGTATCGCCCTCGGCAAGCTGGCCCAGGAAGACCCGTCGTTCCGTGTCAAGACCGACGAAGAGTCCGGTCAGACCATCATCTCCGGCATGGGCGAGTTGCACCTGGACATCATCGTCGACCGCATGAAGCGCGAGTTCGGTGTCGAGGCCAACATCGGCAAGCCGCAGGTTGCCTACCGTGAGACCATCACCAAGGACAACGTGGAGATCGAAGGCAAGTT  >  NC_008463/756882‑757178
                                                                                                                                                    |                                                                                                                                                    
 gAGCGCATGGACTTCCCCGAGCCGGTAATCTCGGTCGCTGTAGAGCCGAAGACCAAGGCTGACCAGGAGAAGATGGGTATCGCCCTCGGCAAGCTGGCCCAGGAAGACCCGTCGTTCCGTGTCAAGACCGACGAAGAGTCCGGTCAGGcc                                                                                                                                                    >  1:8119276/1‑150 (MQ=255)
   gcgcATGGACTTCCCCGAGCCGGTAATCTCGGTCGCTGTAGAGCCGAAGACCAAGGCTGACCAGGAGAAGATGGGTATCGCCCTCGGCAAGCTGGCCCAGGAAGACCCGTCGTTCCGTGTCAAGACCGACGAAGAGTCCGGTCAGGCcat                                                                                                                                                  >  1:8257254/1‑150 (MQ=255)
     gcATGGACTTCCCCGAGCCGGTAATCTCGGTCGCTGTAGAGCCGAAGACCAAGGCTGACCAGGAGAAGATGGGTATCGCCCTCGGCAAGCTGGCCCAGGAAGACCCGTCGTTCCGTGTCAAGACCGACGAAGAGTCCGGTCAGGCcatca                                                                                                                                                <  1:4960354/150‑1 (MQ=255)
       aTGGACTTCCCCGAGCCGGTAATCTCGGTCGCTGTAGAGCCGAAGACCAAGGCTGACCAGGAGAAGATGGGTATCGCCCTCGGCAAGCTGGCCCAGGAAGACCCGTCGTTCCGTGTCAAGACCGACGAAGAGTCCGGTCAGGCCatcatc                                                                                                                                              <  1:10004346/150‑1 (MQ=255)
         ggACTTCCCCGAGCCGGTAATCTCGGTCGCTGTAGAGCCGAAGACCAAGGCTGACCAGGAGAAGATGGGTATCGCCCTCGGCAAGCTGGCCCAGGAAGACCCGTCGTTCCGTGTCAAGACCGACGAAGAGTCCGGTCAGGCCATCAtctc                                                                                                                                            >  2:7148884/1‑150 (MQ=255)
         ggACTTCCCCGAGCCGGTAATCTCGGTCGCTGTAGAGCCGAAGACCAAGGCTGACCAGGAGAAGATGGGTATCGCCCTCGGCAAGCTGGCCCAGGAAGACCCGTCGTTCCGTGTCAAGACCGACGAAGAGTCCGGTCAGACCATCAtctc                                                                                                                                            >  1:7654353/1‑150 (MQ=255)
          gACTTCCCCGAGCCGGTAATCTCGGTCGCTGTAGAGCCGAAGACCAAGGCTGACCAGGAGAAGATGGGTATCGCCCTCGGCAAGCTGGCCCAGGAAGACCCGTCGTTCCGTGTCAAGACCGACGAAGAGTCCGGTCAGACCATCATCTcc                                                                                                                                           <  2:7104090/150‑1 (MQ=255)
          gACTTCCCCGAGCCGGTAATCTCGGTCGCTGTAGAGCCGAAGACCAAGGCTGACCAGGAGAAGATGGGTATCGCCCTCGGCAAGCTGGCCCAGGAAGACCCGTCGTTCCGTGTCAAGACCGACGAAGAGTCCGGTCAGACCATCATCTcc                                                                                                                                           >  1:3575710/1‑150 (MQ=255)
           aCTTCCCCGAGCCGGTAATCTCGGTCGCTGTAGAGCCGAAGACCAAGGCTGACCAGGAGAAGATGGGTATCGCCCTCGGCAAGCTGGCCCAGGAAGACCCGTCGTTCCGTGTCAAGACCGACGAAGAGTCCGGTCAGGCCATCATCTCCg                                                                                                                                          <  1:1752300/150‑1 (MQ=255)
            cTTCCCCGAGCCGGTAATCTCGGTCGCTGTAGAGCCGACGACCAAGGCTGACCAGGAGAAGATGGGTATCGCCCTCGGCAAGCTGGCCCAGGAAGACCCGTCGTTCCGTGTCAAGACCGACGAAGAGTCCGGTCAGGCCATCATCTCCgg                                                                                                                                         <  2:10627199/150‑1 (MQ=255)
            cTTCCCCGAGCCGGTAATCTCGGTCGCTGTAGAGCCGAAGACCAAGGCTGACCAGGAGAAGATGGGTATCGCCCTCGGCAAGCTGGCCCAGGAAGACCCGTCGTTCCGTGTCAAGACCGACGAAGAGTCCGGTCAGGCCATCATCTCCgg                                                                                                                                         >  2:8055997/1‑150 (MQ=255)
             ttCCCCGAGCCGGTAATCTCGGTCGCTGTAGAGCCGAAGACCAAGGCTGACCAGGAGAAGATGGGTATCGCCCTCGGCAAGCTGGCCCAGGAAGACCCGTCGTTCCGTGTCAAGACCGACGAAGAGTCCGGTCAGGCCATCATCTCCGGc                                                                                                                                        >  1:3749849/1‑150 (MQ=255)
              tCCCCGAGCCGGTAATCTCGGTCGCTGTAGAGCCGAAGACCAAGGCTGACCAGGAGAAGATGGGTATCGCCCTCGGCAAGCTGGCCCAGGAAGACCCGTCGTTCCGTGTCAAGACCGACGAAGAGTCCGGTCAGGCCATCATCTCCGGCa                                                                                                                                       >  1:1178923/1‑150 (MQ=255)
                cccGAGCCGGTAATCTCGGTCGCTGTAGAGCCGAAGACCAAGGCTGACCAGGAGAAGATGGGTATCGCCCTCGGCAAGCTGGCCCAGGAAGACCCGTCGTTCCGTGTCAAGACCGACGAAGAGTCCGGTCAGGCCATCATCTCCGGCATg                                                                                                                                     <  2:6017807/150‑1 (MQ=255)
                     gCCGGTAATCTCGGTCGCTGTAGAGCCGAAGACCAAGGCTGACCAGGAGAAGATGGGTATCGCCCTCGGCAAGCTGGCCCAGGAAGACCCGTCGTTCCGTGTCAAGACCGACGAAGAGTCCGGTCAGGCCATCATCTCCGGCATGGGCGa                                                                                                                                >  1:6616038/1‑150 (MQ=255)
                      ccGGTAATCTCGGTCGCTGTAGAGCCGAAGACCAAGGCTGACCAGGAGAAGATGGGTATCGCCCTCGGCAAGCTGGCCCAGGAAGACCCGTCGTTCCGTGTCAAGACCGACGAAGAGTCCGGTCAGGCCATCATCTCCGGCATGGGCGAg                                                                                                                               <  1:10635494/150‑1 (MQ=255)
                       cGGTAATCTCGGTCGCTGTAGAGCCGAAGACCAAGGCTGACCAGGAGAAGATGGGTATCGCCCTCGGCAAGCTGGCCCAGGAAGACCCGTCGTTCCGTGTCAAGACCGACGAAGAGTCCGGTCAGGCCATCATCTCCGGCATGGGCGAGt                                                                                                                              >  2:1491681/1‑150 (MQ=255)
                       cGGTAATCTCGGTCGCTGTAGAGCCGAAGACCAAGGCTGACCAGGAGAAGATGGGTATCGCCCTCGGCAAGCTGGCCCAGGAAGACCCGTCGTTCCGTGTCAAGACCGACGAAGAGTCCGGTCAGGCCATCATCTCCGGCATGGGCGAGt                                                                                                                              <  2:4244199/150‑1 (MQ=255)
                        ggTAATCTCGGTCGCTGTAGAGCCGAAGACCAAGGCTGACCAGGAGAAGATGGGTATCGCCCTCGGCAAGCTGGCCCAGGAAGACCCGTCGTTCCGTGTCAAGACCGACGAAGAGTCCGGTCAGACCATCATCTCCGGCATGGGCGAGtt                                                                                                                             >  1:9957875/1‑150 (MQ=255)
                          tAATCTCGGTCGCTGTAGAGCCGAAGACCAAGGCTGACCAGGAGAAGATGGGTATCGCCCTCGGCAAGCTGGCCCAGGAAGACCCGTCGTTCCGTGTCAAGACCGACGAAGAGTCCGGTCAGGCCATCATCTCCGGCATGGGCGAGTTGc                                                                                                                           <  1:7274103/150‑1 (MQ=255)
                           aaTCTCGGTCGCTGTAGAGCCGAAGACCAAGGCTGACCAGGAGAAGATGGGTATCGCCCTCGGCAAGCTGGCCCAGGAAGACCCGTCGTTCCGTGTCAAGACCGACGAAGAGTCCGGTCAGGCCATCATCTCCGGCATGGGCGAGTTGCa                                                                                                                          >  1:4202951/1‑150 (MQ=255)
                           aaTCTCGGTCGCTGTAGAGCCGAAGACCAAGGCTGACCAGGAGAAGATGGGTATCGCCCTCGGCAAGCTGGCCCAGGAAGACCCGTCGTTCCGTGTCAAGACCGACGAAGAGTCCGGTCAGGCCATCATCTCCGGCATGGGCGAGTTGCa                                                                                                                          >  1:2509616/1‑150 (MQ=255)
                            aTCTCGGTCGCTGTAGAGCCGAAGACCAAGGCTGACCAGGAGAAGATGGGTATCGCCCTCGGCAAGCTGGCCCAGGAAGACCCGTCGTTCCGTGTCAAGACCGACGAAGAGTCCGGTCAGGCCATCATCTCCGGCATGGGCGAGTTGCAc                                                                                                                         >  1:7161395/1‑150 (MQ=255)
                              ctcGGTCGCTGTAGAGCCGAAGACCAAGGCTGACCAGGAGAAGATGGGTATCGCCCTCGGCAAGCTGGCCCAGGAAGACCCGTCGTTCCGTGTCAAGACCGACGAAGAGTCCGGTCAGGCCATCATCTCCGGCATGGGCGAGTTGCACCt                                                                                                                       <  2:6715115/150‑1 (MQ=255)
                                   tCGCTGTAGAGCCGAAGACCAAGGCTGACCAGGAGAAGATGGGTATCGCCCTCGGCAAGCTGGCCCAGGAAGACCCGTCGTTCCGTGTCAAGACCGACGAAGAGTCCGGTCAGGCCATCATCTCCGGCATGGGCGAGTTGCACCTGGAca                                                                                                                  <  1:7796239/150‑1 (MQ=255)
                                   tCGCTGTAGAGCCGAAGACCAAGGCTGACCAGGAGAAGATGGGTATCGCCCTCGGCAAGCTGGCCCAGGAAGACCCGTCGTTCCGTGTCAAGACCGACGAAGAGTCCGGTCAGGCCATCATCTCCGGCATGGGCGAGTTGCACCTGGAca                                                                                                                  <  1:6094857/150‑1 (MQ=255)
                                     gCTGTAGAGCCGAAGACCAAGGCTGACCAGGAGAAGATGGGTATCGCCCTCGGCAAGCTGGCCCAGGAAGACCCGTCGTTCCGTGTCAAGACCGACGAAGAGTCCGGTCAGGCCATCATCTCCGGCATGGGCGAGTTGCACCTGGAcatc                                                                                                                >  1:9284352/1‑150 (MQ=255)
                                       tGTAGAGCCGAAGACCAAGGCTGACCAGGAGAAGATGGGTATCGCCCTCGGCAAGCTGGCCCAGGAAGACCCGTCGTTCCGTGTCAAGACCGACGAAGAGTCCGGTCAGGCCATCATCTCCGGCATGGGCGAGTTGCACCTGGAcatcat                                                                                                              >  1:1436118/1‑150 (MQ=255)
                                          agagCCGAAGACCAAGGCTGACCAGGAGAAGATGGGTATCGCCCTCGGCAAGCTGGCCCAGGAAGACCCGTCGTTCCGTGTCAAGACCGACGAAGAGTCCGGTCAGGCCATCATCTCCGGCATGGGCGAGTTGCACCTGGACATCAtcgt                                                                                                           <  2:3705311/150‑1 (MQ=255)
                                          agagCCGAAGACCAAGGCTGACCAGGAGAAGATGGGTATCGCCCTCGGCAAGCTGGCCCAGGAAGACCCGTCGTTCCGTGTCAAGACCGACGAAGAGTCCGGTCAGGCCATCATCTCCGGCATGGGCGAGTTGCACCTGGACATCAtcgt                                                                                                           >  1:4434837/1‑150 (MQ=255)
                                           gagCCGAAGACCAAGGCTGACCAGGAGAAGATGGGTATCGCCCTCGGCAAGCTGGCCCAGGAAGACCCGTCGTTCCGTGTCAAGACCGACGAAGAGTCCGGTCAGGCCATCATCTCCGGCATGGGCGAGTTGCACCTGGACATCAtcgtc                                                                                                          <  2:8164582/150‑1 (MQ=255)
                                           gagCCGAAGACCAAGGCTGACCAGGAGAAGATGGGTATCGCCCTCGGCAAGCTGGCCCAGGAAGACCCGTCGTTCCGTGTCAAGACCGACGAAGAGTCCGGTCAGGCCATCATCTCCGGCATGGGCGAGTTGCACCTGGACATCAtcgtc                                                                                                          >  1:6987596/1‑150 (MQ=255)
                                             gCCGAAGACCAAGGCTGACCAGGAGAAGATGGGTATCGCCCTCGGCAAGCTGGCCCAGGAAGACCCGTCGTTCCGTGTCAAGACCGACGAAGAGTCCGGTCAGGCCATCATCTCCGGCATGGGCGAGTTGCACCTGGACCTCATCGTCGa                                                                                                        >  2:5390282/1‑150 (MQ=255)
                                             gCCGAAGACCAAGGCTGACCAGGAGAAGATGGGTATCGCCCTCGGCAAGCTGGCCCAGGAAGACCCGTCGTTCCGTGTCAAGACCGACGAAGAGTCCGGTCAGGCCATCATCTCCGGCATGGGCGAGTTGCACCTGGACATCATCGTCGa                                                                                                        >  2:7982753/1‑150 (MQ=255)
                                              ccGAAGACCAAGGCTGACCAGGAGAAGATGGGTATCGCCCTCGGCAAGCTGGCCCAGGAAGACCCGTCGTTCCGTGTCAAGACCGACGAAGAGTCCGGTCAGGCCATCATCTCCGGCATGGGCGAGTTGCACCTGGACATCATCGTCGAc                                                                                                       <  1:4240494/150‑1 (MQ=255)
                                               cGAAGACCAAGGCTGACCAGGAGAAGATGGGTATCGCCCTCGGCAAGCTGGCCCAGGAAGACCCGTCGTTCCGTGTCAAGACCGACGAAGAGTCCGGTCAGGCCATCATCTCCGGCATGGGCGAGTTGCACCTGGACATCATCGTCGAcc                                                                                                      >  1:977914/1‑150 (MQ=255)
                                                gAAGACCAAGGCTGACCAGGAGAAGATGGGTATCGCCCTCGGCAAGCTGGCCCAGGAAGACCCGTCGTTCCGTGTCAAGACCGACGAAGAGTCCGGTCAGGCCATCATCTCCGGCATGGGCGAGTTGCACCTGGACATCATCGTCGACCg                                                                                                     <  2:520217/150‑1 (MQ=255)
                                                gAAGACCAAGGCTGACCAGGAGAAGATGGGTATCGCCCTCGGCAAGCTGGCCCAGGAAGACCCGTCGTTCCGTGTCAAGACCGACGAAGAGTCCGGTCAGGCCATCATCTCCGGCATGGGCGAGTTGCACCTGGACATCATCGTCGACCg                                                                                                     >  2:7559717/1‑150 (MQ=255)
                                                 aaGACCAAGGCTGACCAGGAGAAGATGGGTATCGCCCTCGGCAAGCTGGCCCAGGAAGACCCGTCGTTCCGTGTCAAGACCGACGAAGAGTCCGGTCAGGCCATCATCTCCGGCATGGGCGAGTTGCACCTGGACATCATCGTCGACCGc                                                                                                    <  2:9115501/150‑1 (MQ=255)
                                                    aCCAAGGCTGACCAGGAGAAGATGGGTATCGCCCTCGGCAAGCTGGCACAGGAAGACCCGTCGTTCCGTGTCAAGACCGACGAAGAGTCCGGTCAGGCCAACATCTCCGGCATGGGCGAGATGCACCTGGACATCATCGTCGACCGCATg                                                                                                 >  2:2646386/1‑150 (MQ=255)
                                                     ccAAGGCTGACCAGGAGAAGATGGGTATCGCCCTCGGCAAGCTGGCCCAGGAAGACCCGTCGTTCCGTGTCAAGACCGACGAAGAGTCCGGTCAGGCCATCATCTCCGGCATGGGCGAGTTGCACCTGGACATCATCGTCGACCGCATGa                                                                                                <  1:2937440/150‑1 (MQ=255)
                                                       aaGGCTGACCAGGAGAAGATGGGTATCGCCCTCGGCAAGCTGGCCCAGGAAGACCCGTCGTTCCGTGTCAAGACCGACGAAGAGTCCGGTCAGGCCATCATCTCCGGCATGGGCGAGTTGCACCTGGACATCATCGTCGACCGCATGAAg                                                                                              <  1:5704425/150‑1 (MQ=255)
                                                       aaGGCTGACCAGGAGAAGATGGGTATCGCCCTCGGCAAGCTGGCCCAGGAAGACCCGTCGTTCCGTGTCAAGACCGACGAAGAGTCCGGTCAGGCCATCATCTCCGGCATGGGCGAGTTGCACCTGGACATCATCGTCGACCGCATGAAg                                                                                              >  2:1779068/1‑150 (MQ=255)
                                                       aaGGCTGACCAGGAGAAGATGGGTATCGCCCTCCGCAACCTTGCCCCGGAAGACCCCGCGTTCCGTGTCAATACCCACGAAGAGTCCGGTAAAGCCAACATCGACGGCAAGGGCGAGTTGCACCTGGACATCAACGTCGAACGCATGAAg                                                                                              >  2:4832604/1‑150 (MQ=17)
                                                        aGGCTGACCAGGAGAAGATGGGTATCGCCCTCGGCAAGCTGGCCCAGGAAGACCCGTCGTTCCGTGTCAAGACCGACGAAGAGTCCGGTCAGGCCATCATCTCCGGCATGGGCGAGTTGCACCTGGACATCATCGTCGACCGCATGAAgc                                                                                             >  2:579314/1‑150 (MQ=255)
                                                         ggCTGACCAGGAGAAGATGGGTATCGCCCTCGGCAAGCTGGCCCAGGAAGACCCGTCGTTCCGTGTCAAGACCGACGAAGAGTCCGGTCAGGCCATCATCTCCGGCATGGGCGAGTTGCACCTGGACATCATCGTCGACCGCATGAAgcg                                                                                            >  1:7603322/1‑150 (MQ=255)
                                                         ggCTGACCAGGAGAAGATGGGTATCGCCCTCGGCAAGCTGGCCCAGGAAGACCCGTCGTTCCGTGTCAAGACCGACGAAGAGTCCGGTCAGGCCATCATCTCCGGCATGGGCGAGTTGCACCTGGACATCATCGTCGACCGCATGAAgcg                                                                                            <  2:6864315/150‑1 (MQ=255)
                                                         ggCTGACCAGGAGAAGATGGGTATCGCCCTCGGCAAGCTGGCCCAGGAAGACCCGTCGTTCCGTGTCAAGACCGACGAAGAGTCCGGTCAGGCCATCATCTCCGGCATGGGCGAGTTGCACCTGGACATCATCGTCGACCGCATGAAgcg                                                                                            <  1:9009262/150‑1 (MQ=255)
                                                          gCTGACCAGGAGAAGATGGGTATCGCCCTCGGCAAGCTGGCCCAGGAAGACCCGTCGTTCCGTGTCAAGACCGACGAAGAGTCCGGTCAGGCCATCATCTCCGGCATGGGCGAGTTGCACCTGGACATCATCGTCGACCGCATGAAgcgc                                                                                           <  2:2674249/150‑1 (MQ=255)
                                                             gACCAGGAGAAGATGGGTATCGCCCTCGGCAAGCTGGCCCAGGAAGACCCGTCGTTCCGTGTCAAGACCGACGAAGAGTCCGGTCAGGCCATCATCTCCGGCATGGGCGAGTTGCACCTGGACATCATCGTCGACCGCATGAAGCGCGAg                                                                                        >  1:6770592/1‑150 (MQ=255)
                                                             gACCAGGAGAAGATGGGTATCGCCCTCGGCAAGCTGGCCCAGGAAGACCCGTCGTTCCGTGTCAAGACCGACGAAGAGTCCGGTCAGGCCATCATCTCCGGCATGGGCGAGTTGCACCTGGACATCATCGTCGACCGCATGAAGCGCGAg                                                                                        >  2:7206447/1‑150 (MQ=255)
                                                             gACCAGGAGAAGATGGGTATCGCCCTCGGCAAGCTGGCCCAGGAAGACCCGTCGTTCCGTGTCAAGACCGACGAAGAGTCCGGTCAGGCCATCATCTCCGGCATGGGCGAGTTGCACCTGGACATCATCGTCGACCGCATGAAGCGCGAg                                                                                        >  2:928348/1‑150 (MQ=255)
                                                               ccAGGAGAAGATGGGTATCGCCCTCGGCAAGCTGGCCCAGGAAGACCCGTCGTTCCGTGTCAAGACCGACGAAGAGTCCGGTCAGGCCATCATCTCCGGCATGGGCGAGTTGCACCTGGACATCATCGTCGACCGCATGAAGCGCGAGtt                                                                                      <  1:8675723/150‑1 (MQ=255)
                                                                 aGGAGAAGATGGGTATCGCCCTCGGCAAGCTGGCCCAGGAAGACCCGTCGTTCCGTGTCAAGACCGACGAAGAGTCCGGTCAGGCCATCATCTCCGGCATGGGCGAGTTGCACCTGGACATCATCGTCGACCGCATGAAGCGCGAGTTCg                                                                                    <  2:5661651/150‑1 (MQ=255)
                                                                  ggAGAAGATGGGTATCGCCCTCGGCAAGCTGGCCCAGGAAGACCCGTCGTTCCGTGTCAAGACCGACGAAGAGTCCGGTCAGGCCATCATCTCCGGCATGGGCGAGTTGCACCTGGACATCATCGTCGACCGCATGAAGCGCGAGTTCgg                                                                                   <  1:1556713/150‑1 (MQ=255)
                                                                   gagaAGATGGGTATCGCCCTCGGCAAGCTGGCCCAGGAAGACCCGTCGTTCCGTGTCAAGACCGACGAAGAGTCCGGTCAGGCCATCATCTCCGGCATGGGCGAGTTGCACCTGGACATCATCGTCGACCGCATGAAGCGCGAGTTCGgt                                                                                  <  2:7545454/150‑1 (MQ=255)
                                                                    agaagaTGGGTATCGCCCTCGGCAAGCTGGCCCAGGAAGACCCGTCGTTCCGTGTCAAGACCGACGAAGAGTCCGGTCAGGCCATCATCTCCGGCATGGGCGAGTTGCACCTGGACATCATCGTCGACCGCATGAAGCGCGAGTTCGgtg                                                                                 >  1:4901538/1‑150 (MQ=255)
                                                                     gaagaTGGGTATCGCCCTCGGCAAGCTGGCCCAGGAAGACCCGTCGTTCCGTGTCAAGACCGACGAAGAGTCCGGTCAGGCCATCATCTCCGGCATGGGCGAGTTGCACCTGGACATCATCGTCGACCGCATGAAGCGCGAGTTCGgtgt                                                                                <  1:6566668/150‑1 (MQ=255)
                                                                     gaagaTGGGTATCGCCCTCGGCAAGCTGGCCCAGGAAGACCCGTCGTTCCGTGTCAAGACCGACGAAGAGTCCGGTCAGGCCATCATCTCCGGCATGGGCGAGTTGCACCTGGACATCATCGTCGACCGCATGAAGCGCGAGTTCGgtgt                                                                                <  1:2991410/150‑1 (MQ=255)
                                                                      aagaTGGGTATCGCCCTCGGCAAGCTGGCCCAGGAAGACCCGTCGTTCCGTGTCAAGACCGACGAAGAGTCCGGTCAGGCCATCATCTCCGGCATGGGCGAGTTGCACCTGGACATCATCGTCGACCGCATGAAGCGCGAGTTCGGTGTc                                                                               <  1:8537570/150‑1 (MQ=255)
                                                                      aagaTGGGTATCGCCCTCGGCAAGCTGGCCCAGGAAGACCCGTCGTTCCGTGTCAAGACCGACGAAGAGTCCGGTCAGGCCATCATCTCCGGCATGGGCGAGTTGCACCTGGACATCATCGTCGACCGCATGAAGCGCGAGTTCGGTGTc                                                                               <  2:4030475/150‑1 (MQ=255)
                                                                        gaTGGGTATCGCCCTCGGCAAGCTGGCCCAGGAAGACCCGTCGTTCCGTGTCAAGACCGACGAAGAGTCCGGTCAGGCCATCATCTCCGGCATGGGCGAGTTGCACCTGGACATCATCGTCGACCGCATGAAGCGCGAGTTCGGTGTCGa                                                                             >  2:6095513/1‑150 (MQ=255)
                                                                           gggTATCGCCCTCGGCAAGCTGGCCCAGGAAGACCCGTCGTTCCGTGTCAAGACCGACGAAGAGTCCGGTCAGGCCATCATCTCCGGCATGGGCGAGTTGCACCTGGACATCATCGTCGACCGCATGAAGCGCGAGTTCGGTGTCGAGGc                                                                          <  2:3447130/150‑1 (MQ=255)
                                                                             gTATCGCCCTCGGCAAGCTGGCCCAGGAAGACCCGTCGTTCCGTGTCAAGACCGACGAAGAGTCCGGTCAGGCCATCATCTCCGGCATGGGCGAGTTGCACCTGGACATCATCGTCGACCGCATGAAGCGCGAGTTCGGTGTCGAGGCCa                                                                        <  1:2644813/150‑1 (MQ=255)
                                                                             gTATCGCCCTCGGCAAGCTGGCCCAGGAAGACCCGTCGTTCCGTGTCAAGACCGACGAAGAGTCCGGTCAGGCCATCATCTCCGGCATGGGCGAGTTGCACCTGGACATCATCGTCGACCGCATGAAGCGCGAGTTCGGTGTCGAGGCCa                                                                        <  1:5161541/150‑1 (MQ=255)
                                                                                 cGCCCTCGGCAAGCTGGCCCAGGCAGACCCGTCGTTCCGTGTCAAGACCGACGAAGAGTCCGGTCAGGCCATCCTCTCCGGCATGGGCGAGTTGCACCTGGACATCATCGTCGCCCGCATGAAGCGCGAGTTCGGTGTCGAGGCCAACAt                                                                    <  2:9390780/150‑1 (MQ=255)
                                                                                   cccTCGGCAAGCTGGCCCAGGAAGACCCGTCGTTCCGTGTCAAGACCGACGAAGAGTCCGGTCAGGCCATCATCTCCGGCATGGGCGAGTTGCACCTGGACATCATCGTCGACCGCATGAAGCGCGAGTTCGGTGTCGAGGCCAACATCg                                                                  <  2:9729188/150‑1 (MQ=255)
                                                                                   cccTCGGCAAGCTGGCCCAGGAAGACCCGTCGTTCCGTGTCAAGACCGACGAAGAGTCCGGTCAGGCCATCATCTCCGGCATGGGCGAGTTGCACCTGGACATCATCGTCGACCGCATGAAGCGCGAGTTCGGTGTCGAGGCCAACATCg                                                                  >  2:2064214/1‑150 (MQ=255)
                                                                                    ccTCGGCAAGCTGGCCCAGGAAGACCCGTCGTTCCGTGTCAAGACCGACGAAGAGTCCGGTCAGGCCATCATCTCCGGCATGGGCGAGTTGCACCTGGACATCATCGTCGACCGCATGAAGCGCGAGTTCGGTGTCGAGGCCAACATCgg                                                                 >  1:6400867/1‑150 (MQ=255)
                                                                                     cTCGGCAAGCTGGCCCAGGAAGACCCGTCGTTCCGTGTCAAGACCGACGAAGAGTCCGGTCAGGCCATCATCTCCGGCATGGGCGAGTTGCACCTGGACATCATCGTCGACCGCATGAAGCGCGAGTTCGGTGTCGAGGCCAACATCGGc                                                                <  1:2319721/150‑1 (MQ=255)
                                                                                           aaGCTGGCCCAGGAAGACCCGTCGTTCCGTGTCAAGACCGACGAAGAGTCCGGTCAGGCCATCATCTCCGGCATGGGCGAGTTGCACCTGGACATCATCGTCGACCGCATGAAGCGCGAGTTCGGTGTCGAGGCCAACATCGGCAAGCCg                                                          <  1:747298/150‑1 (MQ=255)
                                                                                             gCTGGCCCAGGAAGACCCGTCGTTCCGTGTCAAGACCGACGAAGAGTCCGGTCAGGCCATCATCTCCGGCATGGGCGAGTTGCACCTGGACATCATCGTCGACCGCATGAAGCGCGAGTTCGGTGTCGAGGCCAACATCGGCAAGCCGCa                                                        >  2:6431791/1‑150 (MQ=255)
                                                                                                      ggAAGACCCGTCGTTCCGTGTCAAGACCGACGAAGAGTCCGGTCAGGCCATCATCTCCGGCATGGGCGAGTTGCACCTGGACATCATCGTCGACCGCATGAAGCGCGAGTTCGGTGTCGAGGCCAACATCGGCAAGCCGCAGGTTGCCTa                                               <  1:9474883/150‑1 (MQ=255)
                                                                                                         aGACCCGTCGTTCCGTGTCAAGACCGACGAAGAGTCCGGTCAGGCCATCATCTCCGGCATGGGCGAGTTGCACCTGGACATCATCGTCGACCGCATGAAGCGCGAGTTCGGTGTCGAGGCCAACATCGGCAAGCCGCAGGTTGCCTACCg                                            <  2:8375661/150‑1 (MQ=255)
                                                                                                           aCCCGTCGTTCCGTGTCAAGACCGACGAAGAGTCCGGTCAGACCATCATCTCCGGCATGGGCGAGTTGCACCTGGACATCATCGTCGACCGCATGAAGCGCGAGTTCGGTGTCGAGGCCAACATCGGCAAGCCGCAGGTTGCCTACCGTg                                          >  1:6812639/1‑150 (MQ=255)
                                                                                                            cccGTCGTTCCGTGTCAAGACCGACGAAGAGTCCGGTCAGGCCATCATCTCCGGCATGGGCGAGTTGCACCTGGACATCATCGTCGACCGCATGAAGCGCGAGTTCGGTGTCGAGGCCAACATCGGCAAGCCGCAGGTTGCCTACCGTga                                         >  1:9585939/1‑150 (MQ=255)
                                                                                                            cccGTCGTTCCGTGTCAAGACCGACGAAGAGTCCGGTCAGGCCATCATCTCCGGCATGGGCGAGTTGCACCTGGACATCATCGTCGACCGCATGAAGCGCGAGTTCGGTGTCGAGGCCAACATCGGCAAGCCGCAGGTTGCCTACCGTga                                         >  1:3961648/1‑150 (MQ=255)
                                                                                                              cgtcgtTCCGTGTCAAGACCGACGAAGAGTCCGGTCAGGCCATCATCTCCGGCATGGGCGAGTTGCACCTGGACATCATCGTCGACCGCATGAAGCGCGAGTTCGGTGTCGAGGCCAACATCGGCAAGCCGCAGGTTGCCTACCGTgaga                                       <  2:8692482/150‑1 (MQ=255)
                                                                                                                   ttCCGTGTCAAGACCGACGAAGAGTCCGGTCAGGCCATCATCTCCGGCATGGGCGAGTTGCACCTGGACATCATCGTCGACCGCATGAAGCGCGAGTTCGGTGTCGAGGCCAACATCGGCAAGCCGCAGGTTGCCTACCGTGAGACCATc                                  <  2:2392660/150‑1 (MQ=255)
                                                                                                                   ttCCGTGTCAAGACCGACGAAGAGTCCGGTCAGGCCATCATCTCCGGCATGGGCGAGTTGCACCTGGACATCATCGTCGACCGCATGAAGCGCGAGTTCGGTGTCGAGGCCAACATCGGCAAGCCGCAGGTTGCCTACCGTGAGACCATc                                  >  2:1013500/1‑150 (MQ=255)
                                                                                                                      cGTGTCAAGACCGACGAAGAGTCCGGTCAGGCCATCATCTCCGGCATGGGCGAGTTGCACCTGGACATCATCGTCGACCGCATGAAGCGCGAGTTCGGTGTCGAGGCCAACATCGGCAAGCCGCAGGTTGCCTACCGTGAGACCATCAcc                               >  1:7137963/1‑150 (MQ=255)
                                                                                                                         gtCAAGACCGACGAAGAGTCCGGTCAGGCCATCATCTCCGGCATGGGCGAGTTGCACCTGGACATCATCGTCGACCGCATGAAGCGCGAGTTCGGTGTCGAGGCCAACATCGGCAAGCCGCAGGTTGCCTACCGTGAGACCATCACCAAg                            >  2:3940260/1‑150 (MQ=255)
                                                                                                                           cAAGACCGACGAAGAGTCCGGTCAGGCCATCATCTCCGGCATGGGCGAGTTGCACCTGGACATCATCGTCGACCGCATGAAGCGCGAGTTCGGTGTCGAGGCCAACATCGGCAAGCCGCAGGTTGCCTACCGTGAGACCATCACCAAGGa                          <  1:3118652/150‑1 (MQ=255)
                                                                                                                           cAAGACCGACGAAGAGTCCGGTCAGGCCATCATCTCCGGCATGGGCGAGTTGCACCTGGACATCATCGTCGACCGCATGAAGCGCGAGTTCGGTGTCGAGGCCAACATCGGCAAGCCGCAGGTTGCCTACCGTGAGACCATCACCAAGGa                          >  1:5448282/1‑150 (MQ=255)
                                                                                                                            aaGACCGACGAAGAGTCCGGTCAGGCCATCATCTCCGGCATGGGCGAGTTGCACCTGGACATCATCGTCGACCGCATGAAGCGCGAGTTCGGTGTCGAGGCCAACATCGGCAAGCCGCAGGTTGCCTACCGTGAGACCATCACCAAGGAc                         >  2:8857519/1‑150 (MQ=255)
                                                                                                                             aGACCGACGAAGAGTCCGGTCAGGCCATCATCTCCGGCATGGGCGAGTTGCACCTGGACATCATCGTCGACCGCATGAAGCGCGAGTTCGGTGTCGAGGCCAACATCGGCAAGCCGCAGGTTGCCTACCGTGAGACCATCACCAAGGACa                        >  1:10232602/1‑150 (MQ=255)
                                                                                                                              gACCGACGAAGAGTCCGGTCAGGCCATCATCTCCGGCATGGGCGAGTTGCACCTGGACATCATCGTCGACCGCATGAAGCGCGAGTTCGGTGTCGAGGCCAACATCGGCAAGCCGCAGGTTGCCTACCGTGAGACCATCACCAAGGACaa                       <  1:5251271/150‑1 (MQ=255)
                                                                                                                              gACCGACGAAGAGTCCGGTCAGGCCATCATCTCCGGCATGGGCGAGTTGCACCTGGACATCATCGTCGACCGCATGAAGCGCGAGTTCGGTGTCGAGGCCAACATCGGCAAGCCGCAGGTTGCCTACCGTGAGACCATCACCAAGGACaa                       <  2:3000892/150‑1 (MQ=255)
                                                                                                                                  gacgaAGAGTCCGGTCAGGCCATCATCTCCGGCATGGGCGAGTTGCACCTGGACATCATCGTCGACCGCATGAAGCGCGAGTTCGGTGTCGAGGCCAACATCGGCAAGCCGCAGGTTGCCTACCGTGAGACCATCACCAAGGACAACGTg                   <  1:9118029/150‑1 (MQ=255)
                                                                                                                                     gaAGAGTCCGGTCAGGCCATCATCTCCGGCATGGGCGAGTTGCACCTGGACATCATCGTCGACCGCATGAAGCGCGAGTTCGGTGTCGAGGCCAACATCGGCAAGCCGCAGGTTGCCTACCGTGAGACCATCACCAAGGACAACGTGgag                >  2:1284915/1‑150 (MQ=255)
                                                                                                                                     gaAGAGTCCGGTCAGACCATCATCTCCGGCATGGGCGAGTTGCACCTGGACATCATCGTCGACCGCATGAAGCGCGAGTTCGGTGTCGAGGCCAACATCGGCAAGCCGCAGGTTGCCTACCGTGAGACCATCACCAAGGACAACGTGgag                <  2:3337801/150‑1 (MQ=255)
                                                                                                                                      aaGAGTCCGGTCAGGCCATCATCTCCGGCATGGGCGAGTTGCACCTGGACATCATCGTCGACCGCATGAAGCGCGAGTTCGGTGTCGAGGCCAACATCGGCAAGCCGCAGGTTGCCTACCGTGAGACCATCACCAAGGACAACGTGgaga               <  2:10507036/150‑1 (MQ=255)
                                                                                                                                      aaGAGTCCGGTCAGGCCATCATCTCCGGCATGGGCGAGTTGCACCTGGACATCATCGTCGACCGCATGAAGCGCGAGTTCGGTGTCGAGGCCAACATCGGCAAGCCGCAGGTTGCCTACCGTGAGACCATCACCAAGGACAACGTGgaga               >  1:217918/1‑150 (MQ=255)
                                                                                                                                       agagTCCGGTCAGGCCATCATCTCCGGCATGGGCGAGTTGCACCTGGACATCATCGTCGACCGCATGAAGCGCGAGTTCGGTGTCGAGGCCAACATCGGCAAGCCGCAGGTTGCCTACCGTGAGACCATCACCAAGGACAACGTGGAGAt              <  1:8402520/150‑1 (MQ=255)
                                                                                                                                           tCCGGTCAGGCCATCATCTCCGGCATGGGCGAGTTGCACCTGGACATCATCGTCGACCGCTTGAAGCGCGAGTTCGGTGTCGAGGCCAACATCGGCAAGCCGCAGGTTGCCTACCGTGAGACCATCACCTAGGACAACGTGGAGATCGaa          <  2:5020384/150‑1 (MQ=255)
                                                                                                                                           tCCGGTCAGGCCATCATCTCCGGCATGGGCGAGTTGCACCTGGACATCATCGTCGACCGCATGAAGCGCGAGTTCGGTGTCGAGGCCAACATCGGCAAGCCGCAGGTTGCCTACCGTGAGACCATCACCAAGGACAACGTGGAGATCGaa          >  2:9619504/1‑150 (MQ=255)
                                                                                                                                            ccGGTCAGGCCATCATCTCCGGCATGGGCGAGTTGCACCTGGACATCATCGTCGACCGCATGAAGCGCGAGTTCGGTGTCGAGGCCAACATCGGCAAGCCGCAGGTTGCCTACCGTGAGACCATCACCAAGGACAACGTGGAGATCGAAg         >  1:1973414/1‑150 (MQ=255)
                                                                                                                                             cGGTCAGGCCATCATCTCCGGCATGGGCGAGTTGCACCTGGACATCATCGTCGACCGCATGAAGCGCGAGTTCGGTGTCGAGGCCAACATCGGCAAGCCGCAGGGTGCCTACCGTGAGACCATCACCAAGGACAACGTGGAGATCGAAgg        <  2:1456218/150‑1 (MQ=255)
                                                                                                                                               gTCAGGCCATCATCTCCGGCATGGGCGAGTTGCACCTGGACATCATCGTCGACCGCATGAAGCGCGAGTTCGGTGTCGAGGCCAACATCGGCAAGCCGCAGGTTGCCTACCGTGAGACCATCACCAAGGACAACGTGGAGATCGAAGGCa      <  1:6220908/150‑1 (MQ=255)
                                                                                                                                               gTCAGGCCATCATCTCCGGCATGGGCGAGTTGCACCTGGACATCATCGTCGACCGCATGAAGCGCGAGTTCGGTGTCGAGGCCAACATCGGCAAGCCGCAGGTTGCCTACCGTGAGACCATCACCAAGGACAACGTGGAGATCGAAGGCa      >  1:4746836/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CGAGCGCATGGACTTCCCCGAGCCGGTAATCTCGGTCGCTGTAGAGCCGAAGACCAAGGCTGACCAGGAGAAGATGGGTATCGCCCTCGGCAAGCTGGCCCAGGAAGACCCGTCGTTCCGTGTCAAGACCGACGAAGAGTCCGGTCAGACCATCATCTCCGGCATGGGCGAGTTGCACCTGGACATCATCGTCGACCGCATGAAGCGCGAGTTCGGTGTCGAGGCCAACATCGGCAAGCCGCAGGTTGCCTACCGTGAGACCATCACCAAGGACAACGTGGAGATCGAAGGCAAGTT  >  NC_008463/756882‑757178

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑