Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 6,070,122 T→C pseudogene (591/1086 nt) PA14_RS27735 → oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084636,070,1220TC100.0% 2370.0 / NA 600pseudogene (591/1086 nt)PA14_RS27735oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (288/312);  total (288/312)

Only 100 of 600 total aligned reads displayed.

CAGCATCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGCTTCCGGGCTGTGGTTTGACCTTGGCCCGCAACCTGGTTGACCAAGGCACTGTGCCTGTTTGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCGAGGTCCTGCTCGACTATCCGCACTGCCAGGTGGCGCTGCGCGCCAGCATGCTCGCTGCCAATCCGGCGCCACGCTT  >  NC_008463/6069972‑6070268
                                                                                                                                                      |                                                                                                                                                  
  gcaTCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCa                                                                                                                                                <  1:9185764/150‑1 (MQ=255)
    aTCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATa                                                                                                                                              >  1:8527767/1‑150 (MQ=255)
       ggCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCg                                                                                                                                           >  1:6506025/1‑150 (MQ=255)
       ggCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCGCGACC‑AGGCACTGTGCCTGTTCGGCATACCg                                                                                                                                           >  1:6740709/1‑150 (MQ=255)
        gCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGc                                                                                                                                          >  2:3794349/1‑150 (MQ=255)
        gCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGc                                                                                                                                          <  1:222663/150‑1 (MQ=255)
         cccGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCa                                                                                                                                         >  2:2101259/1‑150 (MQ=255)
          ccGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAg                                                                                                                                        <  1:6195331/150‑1 (MQ=255)
          ccGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAg                                                                                                                                        >  2:1901136/1‑150 (MQ=255)
          ccGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAg                                                                                                                                        >  2:4761685/1‑150 (MQ=255)
           cGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGc                                                                                                                                       <  1:7300300/150‑1 (MQ=255)
           cGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGc                                                                                                                                       <  2:10671150/150‑1 (MQ=255)
            ggTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCg                                                                                                                                      <  2:6905106/150‑1 (MQ=255)
             gTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGt                                                                                                                                     <  1:7533456/150‑1 (MQ=255)
             gTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGt                                                                                                                                     >  1:10629623/1‑150 (MQ=255)
              tGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTa                                                                                                                                    <  1:9827094/150‑1 (MQ=255)
                 gcaaCTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGACCCCGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGc                                                                                                                                 <  1:10036709/147‑1 (MQ=255)
                 gcTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGc                                                                                                                                 >  1:3834517/1‑150 (MQ=255)
                 gcTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGc                                                                                                                                 >  2:8272926/1‑150 (MQ=255)
                    aCTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACg                                                                                                                              <  2:8725334/150‑1 (MQ=255)
                     cTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACgg                                                                                                                             >  2:6233851/1‑150 (MQ=255)
                         gAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGacac                                                                                                                         <  2:4056127/150‑1 (MQ=255)
                         gAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGacac                                                                                                                         >  1:638743/1‑150 (MQ=255)
                          aGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACAcc                                                                                                                        <  1:3592680/150‑1 (MQ=255)
                          aGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACAcc                                                                                                                        <  2:8843046/150‑1 (MQ=255)
                          aGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACAcc                                                                                                                        <  2:3483059/150‑1 (MQ=255)
                           gTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCt                                                                                                                       <  1:7095935/150‑1 (MQ=255)
                            tCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTg                                                                                                                      >  1:439032/1‑150 (MQ=255)
                               gCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTgcgc                                                                                                                   >  1:4715028/1‑150 (MQ=255)
                                 cATCGAGCGCTTCCGGCCGCAGGTCCGCGACCGCTGGCGCGAACACGTCCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑TCCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCg                                                                                                                 >  2:2616794/1‑150 (MQ=255)
                                  aTCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCgg                                                                                                                >  2:1489098/1‑150 (MQ=255)
                                  aTCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCgg                                                                                                                <  1:9626254/150‑1 (MQ=255)
                                   tCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑CCCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGc                                                                                                               <  1:10282412/150‑1 (MQ=255)
                                        cgcTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTgcgc                                                                                                          >  1:1244235/1‑150 (MQ=255)
                                        cgcTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGGGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTgcgc                                                                                                          >  1:2110896/1‑150 (MQ=255)
                                             ccgtccgCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAggg                                                                                                     >  1:7173705/1‑150 (MQ=255)
                                             ccgtccgCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAggg                                                                                                     <  1:2806400/150‑1 (MQ=255)
                                              cgtccgCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGgc                                                                                                    <  1:1801236/150‑1 (MQ=255)
                                               gtccgCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGgcg                                                                                                   <  1:4537959/150‑1 (MQ=255)
                                               gtccgCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGgcg                                                                                                   >  2:6148663/1‑150 (MQ=255)
                                                  cgCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGgcgcgc                                                                                                <  1:2547712/150‑1 (MQ=255)
                                                    cAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTg                                                                                              >  2:3281613/1‑150 (MQ=255)
                                                     aGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGa                                                                                             >  1:938904/1‑150 (MQ=255)
                                                      ggTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGAc                                                                                            >  2:5918328/1‑150 (MQ=255)
                                                      ggTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGAc                                                                                            <  1:3508631/150‑1 (MQ=255)
                                                       gTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGAcc                                                                                           <  2:7197721/150‑1 (MQ=255)
                                                         ccGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACcga                                                                                         <  2:1040916/150‑1 (MQ=255)
                                                          cgcgACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACcgac                                                                                        <  1:3060047/150‑1 (MQ=255)
                                                             gACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCgacgac                                                                                     <  2:4268432/150‑1 (MQ=255)
                                                             gACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCgacgac                                                                                     >  2:3598753/1‑150 (MQ=255)
                                                                  cTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGtt                                                                                <  2:127434/150‑1 (MQ=255)
                                                                   tGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTc                                                                               >  2:7754817/1‑150 (MQ=255)
                                                                   tGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTc                                                                               >  2:2388393/1‑150 (MQ=255)
                                                                    ggCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCg                                                                              >  1:2485933/1‑150 (MQ=255)
                                                                       gcgAACACGACCTGCCCGGC‑TCCGGGCTGGGGTTCGCCCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTGCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGGGGGCGCGCTGACCGACGACTGGTTCgggg                                                                           >  1:7907990/1‑150 (MQ=255)
                                                                        cgAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCGAGGt                                                                          <  1:9981913/150‑1 (MQ=255)
                                                                           acacGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCGAGGTCCt                                                                       >  2:6598850/1‑150 (MQ=255)
                                                                           acacGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCGAGGTCCt                                                                       <  2:9054512/150‑1 (MQ=255)
                                                                              cGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCGAGGTCCTGCt                                                                    <  2:136103/150‑1 (MQ=255)
                                                                               gACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCGAGGTCCTGCTc                                                                   >  2:7361362/1‑150 (MQ=255)
                                                                                    gCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCGAGGTCCTGCTCGACTa                                                              <  1:7446295/150‑1 (MQ=255)
                                                                                     cccGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCGAGGTCCTGCTCGACTAt                                                             <  2:319806/150‑1 (MQ=255)
                                                                                           ctCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCGAGGTCCTGCTCGACTATCCGCa                                                        >  1:4003100/2‑150 (MQ=255)
                                                                                                ggCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCGAGGTCCTGCTCGACTATCCGCACTGcc                                                   >  2:8571118/1‑150 (MQ=255)
                                                                                                    gtgGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCGAGGTCCTGCTCGACTATCCGCACTGCCAGGt                                               >  1:6083538/1‑150 (MQ=255)
                                                                                                    gtgGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCGAGGTCCTGCTCGACTATCCGCACTGCCAGGt                                               <  1:1108325/150‑1 (MQ=255)
                                                                                                      ggTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCGAGGTCCTGCTCGACTATCCGCACTGCCAGGTgg                                             <  2:9256192/150‑1 (MQ=255)
                                                                                                       gTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCGAGGTCCTGCTCGACTATCCGCACTGCCAGGTGgc                                            >  2:9750945/1‑150 (MQ=255)
                                                                                                       gTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGGTCGAGGTCCTGCTCGACTATCCGCACTGCCAGGTGgc                                            >  2:4535735/1‑150 (MQ=255)
                                                                                                         tcgACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCGAGGTCCTGCTCGACTATCCGCACTGCCAGGTGgcgc                                          >  1:1453719/3‑150 (MQ=255)
                                                                                                            aCCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCGAGGTCCTGCTCGACTATCCGCACTGCCAGGTGGCGCTgc                                       >  2:7933849/1‑150 (MQ=255)
                                                                                                             ccTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCGAGGTCCTGCTCGACTATCCGCACTGCCAGGTGGCGCTgcg                                      >  2:6445709/1‑150 (MQ=255)
                                                                                                               tcggCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATCCCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCGAGGTCCTGCTCGACTATCCGCACTGCCAGGTGGCGCTgcgcg                                    <  1:871156/148‑1 (MQ=255)
                                                                                                                cggCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCGAGGTCCTGCTCGACTATCCGCACTGCCAGGTGGCGCTgcgcgc                                   <  1:3920411/149‑1 (MQ=255)
                                                                                                                  gCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCGAGGTCCTGCTCGACTATCCGCACTGCCAGGTGGCGCTGCGCGCCa                                 >  1:10540088/1‑150 (MQ=255)
                                                                                                                  gCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCGAGGTCCTGCTCGACTATCCGCACTGCCAGGTGGCGCTGCGCGCCa                                 >  2:586417/1‑150 (MQ=255)
                                                                                                                     ccgcaCCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCGAGGTCCTGCTCGACTATCCGCACTGCCAGGTGGCGCTGCGCGCCAGc                               >  1:5275087/5‑150 (MQ=255)
                                                                                                                      cgcaCCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCGAGGTCCTGCTCGACTATCCGCACTGCCAGGTGGCGCTGCGCGCCAGCa                              <  1:4078407/147‑1 (MQ=255)
                                                                                                                      cgcaCCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCGAGGTCCTGCTCGACTATCCGCACTGCCAGGTGGCGCTGCGCGCCAGCa                              <  2:9579744/147‑1 (MQ=255)
                                                                                                                       gcaCCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCGAGGTCCTGCTCGACTATCCGCACTGCCAGGTGGCGCTGCGCGCCAGCAt                             <  2:4367599/148‑1 (MQ=255)
                                                                                                                       gcaCCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCGAGGTCCTGCTCGACTATCCGCACTGCCAGGTGGCGCTGCGCGCCAGCAt                             <  2:9306340/148‑1 (MQ=255)
                                                                                                                         aCCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCGAGGTCCTGCTCGACTATCCGCACTGCCAGGTGGCGCTGCGCGCCAGCATGc                           <  1:4916076/150‑1 (MQ=255)
                                                                                                                          ccTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCGAGGTCCTGCTCGACTATCCGCACTGCCAGGTGGCGCTGCGCGCCAGCATGCt                          >  2:9910594/1‑150 (MQ=255)
                                                                                                                                 cgaccaGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCGAGGTCCTGCTCGACTATCCGCACTGCCAGGTGGCGCTGCGCGCCAGCATGCTCGCTGc                    <  2:5570406/145‑1 (MQ=255)
                                                                                                                                 cgaccaGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCGAGGTCCTGCTCGACTATCCGCACTGCCAGGTGGCGCTGCGCGCCAGCATGCTCGCTGc                    <  2:505876/145‑1 (MQ=255)
                                                                                                                                  gaccaGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCGAGGTCCTGCTCGACTATCCGCACTGCCAGGTGGCGCTGCGCGCCAGCATGCTCGCTGcc                   <  2:6826829/146‑1 (MQ=255)
                                                                                                                                    ccaGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCGAGGTCCTGCTCGACTATCCGCACTGCCAGGTGGCGCTGCGCGCCAGCATGCTCGCTGCCaa                 <  1:2988222/148‑1 (MQ=255)
                                                                                                                                    ccaGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCGAGGTCCTGCTCGACTATCCGCACTGCCAGGTGGCGCTGCGCGCCAGCATGCTCGCTGCCaa                 <  2:4861697/148‑1 (MQ=255)
                                                                                                                                      aGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCGAGGTCCTGCTCGACTATCCGCACTGCCAGGTGGCGCTGCGCGCCAGCATGCTCGCTGCCAATc               >  1:5112754/1‑150 (MQ=255)
                                                                                                                                      aGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCGAGGTCCTGCTCGACTATCCGCACTGCCAGGTGGCGCTGCGCGCCAGCATGCTCGCTGCCAATc               <  2:8071524/150‑1 (MQ=255)
                                                                                                                                       ggCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCGAGGTCCTGCTCGACTATCCGCACTGCCAGGTGGCGCTGCGCGCCAGCATGCTCGCTGCCAATcc              <  2:4201172/150‑1 (MQ=255)
                                                                                                                                       ggCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCGAGGTCCTGCTCGACTATCCGCACTGCCAGGTGGCGCTGCGCGCCAGCATGCTCGCTGCCAATcc              <  2:1652009/150‑1 (MQ=255)
                                                                                                                                         cACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCGAGGTCCTGCTCGACTATCCGCACTGCCAGGTGGCGCTGCGCGCCAGCATGCTCGCTGCCAATCCgg            >  1:81034/1‑150 (MQ=255)
                                                                                                                                             gtgCCTGGTCGGCATACCGCCGCGGATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCGTGGTCCTGCTCGTCTATCCGCACTGCCAGGTGGCGCTGCGCGCCAGCATGCTCGCGGCCACTCCGGCGcc        >  2:2863176/1‑150 (MQ=255)
                                                                                                                                              tgCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCGAGGTCCTGCTCGACTATCCGCACTGCCAGGTGGCGCTGCGCGCCAGCATGCTCGCTGCCAATCCGGCGCCa       <  2:5337943/150‑1 (MQ=255)
                                                                                                                                              tgCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCGAGGTCCTGCTCGACTATCCGCACTGCCAGGTGGCGCTGCGCGCCAGCATGCTCGCTGCCAATCCGGCGCCa       <  1:5942674/150‑1 (MQ=255)
                                                                                                                                                ccTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCGAGGTCCTGCTCGACTATCCGCACTGCCAGGTGGCGCTGCGCGCCAGCATGCTCGCTGCCAATCCGGCGCCACg     >  1:5053538/1‑150 (MQ=255)
                                                                                                                                                ccTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCGAGGTCCTGCTCGACTATCCGCACTGCCAGGTGGCGCTGCGCGCCAGCATGCTCGCTGCCAATCCGGCGCCACg     >  2:10199819/1‑150 (MQ=255)
                                                                                                                                                 cTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCGAGGTCCTGCTCGACTATCCGCACTGCCAGGTGGCGCTGCGCGCCAGCATGCTCGCTGCCAATCCGGCGCCACGc    >  2:5437693/1‑150 (MQ=255)
                                                                                                                                                  tGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCGAGGTCCTGCTCGACTATCCGCACTGCCAGGTGGCGCTGCGCGCCAGCATGCTCGCTGCCAATCCGGCGCCACGCt   >  1:5583149/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                  
CAGCATCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGCTTCCGGGCTGTGGTTTGACCTTGGCCCGCAACCTGGTTGACCAAGGCACTGTGCCTGTTTGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCGAGGTCCTGCTCGACTATCCGCACTGCCAGGTGGCGCTGCGCGCCAGCATGCTCGCTGCCAATCCGGCGCCACGCTT  >  NC_008463/6069972‑6070268

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑