Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 72,440 T→C K132E (AAG→GAG)  PA14_RS00315 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00846372,4400TC100.0% 1646.7 / NA 412K132E (AAG→GAG) PA14_RS00315hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (162/250);  total (162/250)

Only 100 of 417 total aligned reads displayed.

GGCAGCGGTCCGGCGCAGCCGGCGAGGAGCAGCAGCGCGAGCGGGACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTTGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTCCTTGAACTTGAGGTTGAGCTGGCAATCGCGCCAGAGCGGGC  >  NC_008463/72293‑72586
                                                                                                                                                   |                                                                                                                                                  
ggCAGCGGTCCGGCGCAGCCGGCGAGGAGCAGCAGCGCGAGCGCGACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGc                                                                                                                                                  <  2:832593/150‑1 (MQ=255)
 gCAGCGGTCCGGCGCAGCCGGCGAGGAGCAGCAGCGCGAGCGGGACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTcgcg                                                                                                                                                 <  1:9001714/150‑1 (MQ=255)
 gCAGCGGTCCGGCGCAGCCGGCGAGGAGCAGCAGCGCGAGCGGGACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTcgcg                                                                                                                                                 >  2:9433310/1‑150 (MQ=255)
  cAGCGGTCCGGCGCAGCCGGCGAGGAGCAGCAGCGCGAGCGGGACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCgg                                                                                                                                                <  1:6252048/150‑1 (MQ=255)
  cAGCGGTCCGGCGCAGCCGGCGAGGAGCAGCAGCGCGAGCGGGACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCgg                                                                                                                                                <  2:9864302/150‑1 (MQ=255)
   aGCGGTCCGGCGCAGCCGGCGAGGAGCAGCAGCGCGAGCGGGACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGc                                                                                                                                               <  2:9918399/150‑1 (MQ=255)
       gTCCGGCGCAGCCGGCGAGGAGCAGCAGCGCGAGCGGGACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCcgcg                                                                                                                                           >  1:5908925/1‑150 (MQ=255)
          cGGCGCAGCCGGCGAGGAGCAGCAGCGCGAGCGGGACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCgcgcgc                                                                                                                                        >  1:2924316/1‑150 (MQ=255)
           ggCGCAGCCGGCGAGGAGCAGCAGCGCGAGCGGGACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGcc                                                                                                                                       <  1:1305486/150‑1 (MQ=255)
                   cGGCGAGGAGCAGCAGCGCGAGCGGGACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCt                                                                                                                               >  2:9142297/1‑150 (MQ=255)
                    ggCGAGGAGCAGCAGCGCGAGCGGGACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCtg                                                                                                                              >  1:1442426/1‑150 (MQ=255)
                         ggagCAGCAGCGCGAGCGGGACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTc                                                                                                                         <  2:8934500/150‑1 (MQ=255)
                         ggagCAGCAGCGCGAGCGGGACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTc                                                                                                                         <  2:624880/150‑1 (MQ=255)
                             cagcagcGCGAGCGGGACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTgg                                                                                                                     >  1:9708262/1‑150 (MQ=255)
                              agcagcGCGAGCGGGACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGt                                                                                                                    <  1:2252200/150‑1 (MQ=255)
                              agcagcGCGAGCGGGACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGt                                                                                                                    >  2:5876522/1‑150 (MQ=255)
                               gcagcGCGAGCGGGACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTc                                                                                                                   >  1:6742366/1‑150 (MQ=255)
                                cagcGCGAGCGGGACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCg                                                                                                                  >  2:10324068/1‑150 (MQ=255)
                                 agcGCGAGCGGGACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGt                                                                                                                 <  1:2063569/150‑1 (MQ=255)
                                   cgcgAGCGGGACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTACAGCTACTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTag                                                                                                               <  2:6625140/150‑1 (MQ=255)
                                        gCGGGACGAGACGGGTGAGTGGACGCATGGGGCGTGATCAGGATGTCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCGGTTCATACCTTCGCGCAGCGCCCCTCGCGGCCGCGAGCCAATTCCCTGTTTTCCTGGTCGTCGAGCtt                                                                                                          <  2:1073273/150‑1 (MQ=255)
                                             aCGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGccc                                                                                                     <  1:10192070/150‑1 (MQ=255)
                                              cGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCa                                                                                                    <  1:6101189/150‑1 (MQ=255)
                                               gagaCGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAg                                                                                                   <  2:9673111/150‑1 (MQ=255)
                                               gagaCGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAg                                                                                                   >  1:8156546/1‑150 (MQ=255)
                                                agaCGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAgg                                                                                                  >  1:3253130/1‑150 (MQ=255)
                                                  aCGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGa                                                                                                >  1:8856070/1‑150 (MQ=255)
                                                  aCGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGa                                                                                                >  1:10296168/1‑150 (MQ=255)
                                                    gggTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACg                                                                                              <  2:7474549/150‑1 (MQ=255)
                                                       tGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCCGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAAGTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGaa                                                                                           <  1:10142045/150‑1 (MQ=255)
                                                        gAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAg                                                                                          <  1:4601780/150‑1 (MQ=255)
                                                        gAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAg                                                                                          >  1:3561199/1‑150 (MQ=255)
                                                        gAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAg                                                                                          >  1:9494871/1‑150 (MQ=255)
                                                          gTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGcc                                                                                        <  1:4741997/150‑1 (MQ=255)
                                                            ggACGCATGGGGCGGGCTCCGGAGGGCGGTCAGCTCCTAGCAGAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGa                                                                                      <  2:7604515/150‑1 (MQ=255)
                                                               cGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGAtgt                                                                                   <  1:3002703/150‑1 (MQ=255)
                                                                   tGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGccc                                                                               <  2:8453704/150‑1 (MQ=255)
                                                                    ggggCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCg                                                                              <  2:8635963/150‑1 (MQ=255)
                                                                        cgggcTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCt                                                                          <  2:2045593/150‑1 (MQ=255)
                                                                          ggcTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTcc                                                                        >  1:1848168/1‑150 (MQ=255)
                                                                          ggcTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTcc                                                                        <  1:2350937/150‑1 (MQ=255)
                                                                           gcTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCa                                                                       <  1:3931885/150‑1 (MQ=255)
                                                                            cTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAg                                                                      <  1:741786/150‑1 (MQ=255)
                                                                                ggAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCgg                                                                  >  1:3496523/1‑150 (MQ=255)
                                                                                ggAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCgg                                                                  >  1:6417927/1‑150 (MQ=255)
                                                                                ggAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCgg                                                                  <  1:7860598/150‑1 (MQ=255)
                                                                                ggAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCACGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCgg                                                                  >  2:7989030/1‑150 (MQ=255)
                                                                                 gAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGt                                                                 <  2:5739745/150‑1 (MQ=255)
                                                                                      cGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAgcgc                                                            <  2:6819480/150‑1 (MQ=255)
                                                                                        ttCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTg                                                          <  2:4845077/150‑1 (MQ=255)
                                                                                        ttCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTg                                                          <  1:9742906/150‑1 (MQ=255)
                                                                                          cAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGGTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGc                                                        <  1:5733887/150‑1 (MQ=255)
                                                                                           aGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGcc                                                       >  1:4320541/1‑150 (MQ=255)
                                                                                           aGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGcc                                                       >  2:10598491/1‑150 (MQ=255)
                                                                                           aGCTCCTAGCAGAGCGCCCTCGCCTGGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGcc                                                       <  1:9223675/150‑1 (MQ=255)
                                                                                             cTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCgg                                                     <  2:1182254/150‑1 (MQ=255)
                                                                                             cTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATGCCAGGTGGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGCAGCCGATGTCCCCGGCCTCAAGGCGGTAGCGCTGGCCgg                                                     >  2:3244515/1‑150 (MQ=255)
                                                                                               ccTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGgcg                                                   <  1:9123183/150‑1 (MQ=255)
                                                                                                cTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGgcgc                                                  <  2:7226513/150‑1 (MQ=255)
                                                                                                 tAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCACTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGACGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCt                                                 >  2:2076509/1‑150 (MQ=255)
                                                                                                  aGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTg                                                <  2:1859761/150‑1 (MQ=255)
                                                                                                   gCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGa                                               <  2:450645/150‑1 (MQ=255)
                                                                                                    cATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCCGGGACGGAAGCCGATGGGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGaa                                              <  2:7681411/150‑1 (MQ=255)
                                                                                                    cATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGaa                                              <  1:7524383/150‑1 (MQ=255)
                                                                                                      tAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGt                                            >  2:7047219/1‑150 (MQ=255)
                                                                                                      tAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGt                                            <  1:3848665/150‑1 (MQ=255)
                                                                                                            ccTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTCCTTGa                                      <  2:2451881/150‑1 (MQ=255)
                                                                                                             cTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTCCTTGaa                                     <  2:9267038/150‑1 (MQ=255)
                                                                                                             cTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTCCTTGaa                                     <  1:7988630/150‑1 (MQ=255)
                                                                                                              tCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTCCTTGAAc                                    >  1:6635454/1‑150 (MQ=255)
                                                                                                                  cTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTCCTTGAACTTGa                                <  2:3770554/150‑1 (MQ=255)
                                                                                                                  cTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTCCTTGAACTTGa                                <  1:5459980/150‑1 (MQ=255)
                                                                                                                  cTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGGAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGGCCTTGAACTTGa                                >  2:4134872/1‑150 (MQ=255)
                                                                                                                      cgcCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTCCTTGAACTTGAGGtt                            >  1:6676864/1‑150 (MQ=255)
                                                                                                                       gcCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTCCTTGAACTTGAGGTTg                           <  1:1117563/150‑1 (MQ=255)
                                                                                                                        ccGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTCCTTGAACTTGAGGTTGa                          <  1:8324153/150‑1 (MQ=255)
                                                                                                                         cGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTCCTTGAACTTGAGGTTGAg                         <  1:510360/150‑1 (MQ=255)
                                                                                                                         cGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTCCTTGAACTTGAGGTTGAg                         <  1:8773647/150‑1 (MQ=255)
                                                                                                                          gTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTCCTTGAACTTGAGGTTGAGc                        >  1:2663435/1‑150 (MQ=255)
                                                                                                                           ttCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTCCTTGAACTTGAGGTTGAGCt                       >  1:7221661/1‑150 (MQ=255)
                                                                                                                             cAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGa                                               <  2:1693365/124‑1 (MQ=255)
                                                                                                                             cAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGa                                               >  1:1693365/1‑124 (MQ=255)
                                                                                                                             cAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGATGGCGTTGAACTTGAGGTTGAGCTgg                     >  2:5256825/1‑150 (MQ=255)
                                                                                                                             cAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTCCTTGAACTTGAGGTTGAGCTgg                     >  1:5983782/1‑150 (MQ=255)
                                                                                                                              aGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTCGCGCTGGCCGGCGCTGAAGTCCTTGAACTTTCGGTTGAGCTGGc                    >  2:2660723/1‑150 (MQ=255)
                                                                                                                               gACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTCCTTGAACTTGAGGTTGAGCTGGCa                   >  1:3154222/1‑150 (MQ=255)
                                                                                                                               gACCGTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATACCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTCCTTGAACTTGAGGTTGAGCTGGCa                   <  2:10128149/150‑1 (MQ=255)
                                                                                                                                 ccTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTCCTTGAACTTGAGGTTGAGCTGGCAAt                 >  1:6869678/1‑150 (MQ=255)
                                                                                                                                 ccTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGATTCGCCCAGGGACGGACGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTCCTTGAACTTGAGGTTGAGCTGGCAAt                 <  2:3852081/150‑1 (MQ=255)
                                                                                                                                  cTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTCCTTGAACTTGAGGTTGAGCTGGCAATc                <  1:5284500/150‑1 (MQ=255)
                                                                                                                                   ttCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTCCTTGAACTTGAGGTTGAGCTGGCAATcg               <  1:4667686/150‑1 (MQ=255)
                                                                                                                                     cgcgCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTCCTTGAACTTGAGGTTGAGCTGGCAATcgcg             <  1:1054856/150‑1 (MQ=255)
                                                                                                                                        gcAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTCCTTGAACTTGAGGTTGAGCTGGCAATCGCGCCa          >  1:858255/1‑150 (MQ=255)
                                                                                                                                         cAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTCCTTGAACTTGAGGTTGAGCTGGCAATCGCGCCag         >  1:989770/1‑150 (MQ=255)
                                                                                                                                          aGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTCCTTGAACTTGAGGTTGAGCTGGCAATCGCGCCaga        >  1:10012794/1‑150 (MQ=255)
                                                                                                                                             gcCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTCCTTGAACTTGAGGTTGAGCTGGCAATCGCGCCAGAGCg     <  1:3381946/150‑1 (MQ=255)
                                                                                                                                             gcCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTCCTTGAACTTGAGGTTGAGCTGGCAATCGCGCCAGAGCg     >  2:9546764/1‑150 (MQ=255)
                                                                                                                                              ccccTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTCCTTGAACTTGAGGTTGAGCTGGCAATCGCGCCAGAGCgg    <  2:3020047/150‑1 (MQ=255)
                                                                                                                                               cccTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTCCTTGAACTTGAGGTTGAGCTGGCAATCGCGCCAGAGCggg   <  1:2464083/150‑1 (MQ=255)
                                                                                                                                               cccTCGCGGCCGCGCGCCAATACCATGATGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGAAGCGCTGGCAGGCGCTGAAGGCCTTGAACTTGAGGGTGAGCTGGCAATCGCGCCagagagtg   >  2:5983694/1‑146 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GGCAGCGGTCCGGCGCAGCCGGCGAGGAGCAGCAGCGCGAGCGGGACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTTGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTCCTTGAACTTGAGGTTGAGCTGGCAATCGCGCCAGAGCGGGC  >  NC_008463/72293‑72586

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑