Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 1,128,162 N→C intergenic (+23/‑38) PA14_RS05280 → / → PA14_RS05285 DUF1302 domain‑containing protein/DUF1329 domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084631,128,1620NC99.5% 1514.3 / NA 402intergenic (+23/‑38)PA14_RS05280/PA14_RS05285DUF1302 domain‑containing protein/DUF1329 domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base N (0/0);  major base C (207/192);  minor base G (1/1);  total (209/193)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 405 total aligned reads displayed.

GGGTGGACGCGGAGTACCAGAACACCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTNNNANCNGGNCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCCGACGAGGCGGCCAAGCTCGGCACCAGC  >  NC_008463/1128015‑1128310
                                                                                                                                                   |                                                                                                                                                    
gggTGGACGCGGAGTACCAGAACACCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGCACTTCTGATGTACGCCGGCCCGGTGCGACCCgg                                                                                                                                                    >  2:8390072/1‑150 (MQ=255)
   tGGACGCGGAGTACCAGAACACCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCATCGGCATGAACTTATGATGTACGCCGGCCCGGTGCGAACCGGGcc                                                                                                                                                 >  1:1382776/1‑150 (MQ=255)
     gACGCGGAGTACCAGAACACCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCgg                                                                                                                                               <  2:10649585/150‑1 (MQ=255)
      aCGCGGAGTACCAGAACACCTACACCGCGAGCCTCTCCTACGCCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGc                                                                                                                                              <  1:1656463/150‑1 (MQ=255)
       cgcgGATGACCCGAACCCCGACCCCGCGAGCCACTCCTACCCCCACTTCTCCGACGGCAAGGACACCCCCGTGGACGAGCGCGACTTCGACGCGCACAGCGTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGACCCGGGCCGGCg                                                                                                                                             <  2:8465328/150‑1 (MQ=255)
             gTACCAGTACACCTACACCGCGAGCCTATCCTACACCAACTTCTTCTACGTCAAGTACACCACCGTTGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAAATTCTGATTTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAgg                                                                                                                                       <  2:3497698/150‑1 (MQ=255)
               aCCAGAACACCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCCTGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCCGGAc                                                                                                                                     >  1:10029014/1‑150 (MQ=255)
                 cAGAACACCTACCCCGCGAGCCTCTCCTACACCAACTACTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACtt                                                                                                                                   <  1:9098900/150‑1 (MQ=255)
                   gAACACCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTaa                                                                                                                                 >  2:10382078/1‑150 (MQ=255)
                      cacCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGACCCGGGCCGGCGAGCAGGACTTAACGa                                                                                                                              >  2:3668272/1‑150 (MQ=255)
                       acCTACACCGCGAGCCTCTCCTACCCCAACTTCTTCTACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCGCATCTTAGGCATGAACTTGTTATGTTCTCCGGCCCGGTGCGACCAGGGCCGGCGCGCAGGACTTCACGAg                                                                                                                             >  2:9260719/1‑150 (MQ=255)
                        ccTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGAGCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAgg                                                                                                                            >  2:4933280/1‑150 (MQ=255)
                         ctcccccGCGAGCCTCTCCTACACCACCCTCTTCGACGGCAAGTACACCCCCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAggc                                                                                                                           <  1:3221296/145‑2 (MQ=255)
                         cTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGga                                                                                                                           >  2:10073988/1‑150 (MQ=255)
                          tACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCCCCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATTAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGgac                                                                                                                          <  1:8963855/150‑1 (MQ=255)
                          tACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGAAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGAATTAACGAGgac                                                                                                                          >  2:421068/1‑150 (MQ=255)
                           acacCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCGTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGgacg                                                                                                                         >  1:7822750/1‑150 (MQ=255)
                            cacCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGgacga                                                                                                                        >  2:7884580/1‑150 (MQ=255)
                             acCGCGAGCCTCTCCTCCACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGgacgac                                                                                                                       <  1:6481137/150‑1 (MQ=255)
                              ccGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGacgacg                                                                                                                      <  1:5422478/150‑1 (MQ=255)
                               cgcgAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGAcgacga                                                                                                                     <  1:195424/150‑1 (MQ=255)
                                gcgAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAg                                                                                                                    >  2:2230352/1‑150 (MQ=255)
                                 cgAGCCTCTCCTACACCACCTTCGACGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGc                                                                                                                   <  1:1188377/150‑1 (MQ=255)
                                 cgAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATAGCGACTTCGTCGCGCTCAGCGTCGGGCTGAACTTCTGATGGAAGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGc                                                                                                                   >  1:1122188/1‑150 (MQ=255)
                                  gAGCCACTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCg                                                                                                                  <  2:3274267/150‑1 (MQ=255)
                                    gCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATTAACTTCTGATGTAAGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACGTAACGAGGACGACCAGCGaa                                                                                                                >  1:1901113/1‑150 (MQ=255)
                                    gCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGaa                                                                                                                <  1:10495330/150‑1 (MQ=255)
                                     ccTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGGGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATTAACTTCTGAGGTACGCCGGCCCGGGGCTAACGGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAAt                                                                                                               <  1:3579829/150‑1 (MQ=255)
                                      ctctCCTACACCAACTTCTTCGACGGCACGTACACCACCGTGGACGATCGCGACTTCGACGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATg                                                                                                              <  2:1211403/150‑1 (MQ=255)
                                       tctcCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGa                                                                                                             >  2:6958059/1‑150 (MQ=255)
                                        ctcCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGaa                                                                                                            >  2:10362044/1‑150 (MQ=255)
                                          ccTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGaaaa                                                                                                          <  1:8419725/150‑1 (MQ=255)
                                             acacCCACTTCTTCGACGGCACGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGTAaacaa                                                                                                       >  2:5743319/1‑150 (MQ=255)
                                             acacCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAaacaa                                                                                                       >  1:8671952/1‑150 (MQ=255)
                                              cacCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAaacaac                                                                                                      <  1:5687249/150‑1 (MQ=255)
                                               acCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACg                                                                                                     <  1:7390123/150‑1 (MQ=255)
                                               acCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACGTAACGAGGACGGCGAGCGCATGAAAACAACg                                                                                                     >  1:6107286/1‑150 (MQ=255)
                                                   acgtcttcGACGGCAAGTACGCCTCCGTGGACGATCGCGACTTCGTCGCGCGCAGCGTCGGCGTGCACTTCTGCTGTACGACGGGCCGGTGCGAACGGGGGGGGCGCGCCGGACTTATCGAGGCCGACGAGCGACTGAACACACCGAAgg                                                                                                 >  2:7357735/4‑149 (MQ=255)
                                                     ttcttcGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCCGCTTCGGCATTAACTTCTGATGGACGCCGGCCCGGTGCGACCCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGACAACAACGAAGAtt                                                                                               >  2:7263698/1‑150 (MQ=255)
                                                        ttcGACGGCAAGTACACCACCTTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGAttctt                                                                                            <  1:8826092/150‑1 (MQ=255)
                                                        ttcGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGAttctt                                                                                            >  1:6928732/1‑150 (MQ=255)
                                                        ttcGACGGCAAGTACACCACCGTGGACGATCGCGACTACGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGACAGAAAACAACGAAGAttctt                                                                                            >  2:4843495/1‑150 (MQ=255)
                                                           gACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTg                                                                                         <  2:9490536/150‑1 (MQ=255)
                                                            aCGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGc                                                                                        <  1:7734197/150‑1 (MQ=255)
                                                            aCGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTATGc                                                                                        >  1:4238718/1‑150 (MQ=255)
                                                                cAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATAc                                                                                    >  2:4256761/1‑150 (MQ=255)
                                                                cAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACGTAACGAGGACGACGAGCGACTGAAAACAACGAAGATTCTTCTGCATAc                                                                                    >  1:7663088/1‑150 (MQ=255)
                                                                cAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGGTCGCCCGCCCGGTGCGGAACGGGCCGGCGAGCCGGCCTTAACGAGGACGACGAGCGAAAGAAAACCACGAAGATTCATCTGCATaa                                                                                    >  1:10334637/1‑149 (MQ=255)
                                                                 aaGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATAcc                                                                                   >  2:4135735/1‑150 (MQ=255)
                                                                 aaGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATAcc                                                                                   <  1:2632973/150‑1 (MQ=255)
                                                                 aaGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATAcc                                                                                   <  1:5843834/150‑1 (MQ=255)
                                                                 aaGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGAGGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATAcc                                                                                   <  2:9077765/150‑1 (MQ=255)
                                                                 aaGTACACCACCGTGGACGATCGCGACGTCGGCGCGCTCAGCGTCGGCATGAACTTCGGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGTGGAGGACGTAACGAGGACGTCGAGCGAATGAAAACAACGAAGATTCTTCTGCATAcc                                                                                   >  1:445787/1‑150 (MQ=255)
                                                                  aGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCg                                                                                  <  2:8120431/150‑1 (MQ=255)
                                                                     acacCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGgtg                                                                               <  2:8604491/150‑1 (MQ=255)
                                                                      caccacCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCCGGCCTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCGTCTGCATACCGgtgt                                                                              >  2:7209073/1‑150 (MQ=255)
                                                                      caccacCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGgtgt                                                                              <  2:4439916/150‑1 (MQ=255)
                                                                        ccaccGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTAc                                                                            >  2:1014727/1‑150 (MQ=255)
                                                                          accGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTg                                                                          <  2:663072/150‑1 (MQ=255)
                                                                            cGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGc                                                                        <  1:4718064/150‑1 (MQ=255)
                                                                             gTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACGTCTGATGTTCGCCGGCCCGGTGCGCACCGGGCCGGCGAGGAGGACTTTCCGTGGACGACGAGCGAAGGAAAACAACGAAGATTGTTCTGCATACCGGTGTACTGGcc                                                                       >  2:9193919/1‑150 (MQ=255)
                                                                             gTGGACGATCGCGACGTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCAGCATACCGGTGTACAGGcc                                                                       >  2:4570048/1‑150 (MQ=255)
                                                                                gACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTg                                                                    <  2:5849852/150‑1 (MQ=255)
                                                                                 aCGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGa                                                                   >  2:7848619/1‑150 (MQ=255)
                                                                                       gcgACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCctgc                                                             >  1:5741585/1‑150 (MQ=255)
                                                                                       gcgACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCctgc                                                             <  1:3798844/150‑1 (MQ=255)
                                                                                        cgACTTCGTCGCGCTCAGCATCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAAAAACGAAGATTCTTCTGCATACCGGAGTACTGGCCCTGAGCctgct                                                            >  2:3838070/1‑150 (MQ=255)
                                                                                             tcgtcgCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACATAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATTCCTGTGTACTGGCCCTGAGCCTGCTGGCCa                                                       >  2:6161649/1‑150 (MQ=255)
                                                                                              cgtcgCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCAc                                                      <  2:1975066/150‑1 (MQ=255)
                                                                                                tcgCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAATATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCAccc                                                    >  2:3417043/1‑150 (MQ=255)
                                                                                                   cgcTCAGCTTCGGCATTAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAgg                                                 <  2:2924593/150‑1 (MQ=255)
                                                                                                      tCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGa                                              <  1:3982918/150‑1 (MQ=255)
                                                                                                      tCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGa                                              >  2:5048816/1‑150 (MQ=255)
                                                                                                      tCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGa                                              >  1:6259032/1‑150 (MQ=255)
                                                                                                         gCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATgg                                           >  1:2760045/1‑150 (MQ=255)
                                                                                                          cTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATggc                                          >  2:5303337/1‑150 (MQ=255)
                                                                                                          cTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATggc                                          <  1:9979484/150‑1 (MQ=255)
                                                                                                            tCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATggcgg                                        >  1:9493118/1‑150 (MQ=255)
                                                                                                             cGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATggcggc                                       <  1:2191503/150‑1 (MQ=255)
                                                                                                              ggCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGgcggcg                                      <  1:9251396/150‑1 (MQ=255)
                                                                                                                 aTGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTa                                   <  1:6143595/150‑1 (MQ=255)
                                                                                                                  tGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTAt                                  >  2:1471265/1‑150 (MQ=255)
                                                                                                                   gAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATc                                 >  2:2774467/1‑150 (MQ=255)
                                                                                                                    aaCTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATcc                                >  2:8249150/1‑150 (MQ=255)
                                                                                                                     aCTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCATGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATccg                               <  2:9816424/150‑1 (MQ=255)
                                                                                                                     aCTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATccg                               >  2:5457432/1‑150 (MQ=255)
                                                                                                                      cTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATccgc                              <  1:8235527/150‑1 (MQ=255)
                                                                                                                        tCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATccgccg                            <  2:8772490/150‑1 (MQ=255)
                                                                                                                        tCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGGACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATccgccg                            >  2:1821236/1‑150 (MQ=255)
                                                                                                                           gaggTACGCCGGCCCGGTGCGACCCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCcgacg                         <  1:6658979/147‑1 (MQ=255)
                                                                                                                            aTGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCcgacga                        >  1:3361967/1‑150 (MQ=255)
                                                                                                                               tACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCCGACGAggc                     <  1:9762618/150‑1 (MQ=255)
                                                                                                                                   ccGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCCGACGAGGCGGcc                 <  2:1382776/150‑1 (MQ=255)
                                                                                                                                    cGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCCGACGAGGCGGCCa                <  1:7080355/150‑1 (MQ=255)
                                                                                                                                      gCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCCGACGAGGCGGCCAAg              >  1:4579984/1‑150 (MQ=255)
                                                                                                                                        ccGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCCGACGAGGCGGCCAAGCt            <  2:643151/150‑1 (MQ=255)
                                                                                                                                             gcgaaCCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCCGACGAGGCGGCCAAGCTCGGCa       <  1:5069196/147‑1 (MQ=255)
                                                                                                                                              cgaaCCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCCGACGAGGCGGCCAAGCTCGGCAc      <  1:294657/148‑1 (MQ=255)
                                                                                                                                                aaCCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCCGACGAGGCGGCCAAGCTCGGCACCa    <  1:4129201/150‑1 (MQ=255)
                                                                                                                                                 accGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCCGACGAGGCGGCCAAGCTCGGCACCAg   <  1:10203345/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
GGGTGGACGCGGAGTACCAGAACACCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTNNNANCNGGNCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCCGACGAGGCGGCCAAGCTCGGCACCAGC  >  NC_008463/1128015‑1128310

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑