Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 1,375,947 A→G intergenic (+18/‑38) PA14_RS06470 → / → PA14_RS06475 PspA/IM30 family protein/DUF1449 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084631,375,9470AG100.0% 1599.8 / NA 402intergenic (+18/‑38)PA14_RS06470/PA14_RS06475PspA/IM30 family protein/DUF1449 family protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (191/211);  total (191/211)

Only 100 of 407 total aligned reads displayed.

GGCCGAACTGGCCAGCGCGGCGGCGCCGGACGATGCGCTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCAAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTCGCGATCATCTACTGGGGCATCGTCGCCCTGGGCATGGTCGAGA  >  NC_008463/1375800‑1376093
                                                                                                                                                   |                                                                                                                                                  
   cGAACTGGCCAGCGCGGCGGCGCCGGACGATGCGCTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGc                                                                                                                                               >  1:9238768/1‑150 (MQ=255)
    gAACTGGCCAGCGCGGCGGCGCCGGACGATGCGCTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCg                                                                                                                                              <  1:5949597/150‑1 (MQ=255)
     aaCTGGCCAGCGCGGCGGCGCCGGACGATGCGCTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCgg                                                                                                                                             >  1:8013919/1‑149 (MQ=255)
      aCTGGCCAGCGCGGCGGCGCCGGACGATGCGCTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTc                                                                                                                                            >  2:2472942/1‑150 (MQ=255)
         ggCCAGCGCGGCGGCGCCGGACGATGCGCTTGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGAGTGGATGGCGTGCTGGCGCGCCTGAAGGACAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCgg                                                                                                                                         >  1:190983/1‑150 (MQ=255)
                gcggcggcgCCGGACGATGCGCTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGtt                                                                                                                                  <  1:6367428/150‑1 (MQ=255)
                  ggcggcgCCGGACGATGCGCTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGtttt                                                                                                                                >  2:9201138/1‑150 (MQ=255)
                   gcggcgCCGGACGATGCGCTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGCAGGCGTCCGGGCCCGttttt                                                                                                                               >  1:3032156/1‑150 (MQ=255)
                     ggcgCCGGACGATGCGCTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCa                                                                                                                             >  1:8931697/1‑150 (MQ=255)
                     ggcgCCGGACGATGCGCTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCa                                                                                                                             >  2:5131264/1‑150 (MQ=255)
                          cGGACGATGCGCTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGAt                                                                                                                        <  1:6963831/150‑1 (MQ=255)
                          cGGACGATGCGCTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGAt                                                                                                                        <  1:5015637/150‑1 (MQ=255)
                          cGGACGATGCGCTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGAt                                                                                                                        <  2:1884587/150‑1 (MQ=255)
                           ggACGATGCGCTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATa                                                                                                                       <  2:3407315/150‑1 (MQ=255)
                            gACGATGCGCTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCTGGGATag                                                                                                                      >  1:10011677/1‑150 (MQ=255)
                            gACGATGCGCTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATag                                                                                                                      <  2:2322679/150‑1 (MQ=255)
                            gACGATGCGCTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATag                                                                                                                      >  2:6096561/1‑150 (MQ=255)
                                aTGCGCTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCACCAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCg                                                                                                                  >  2:2689766/1‑150 (MQ=255)
                                aTGCGCTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCg                                                                                                                  >  2:1367079/1‑150 (MQ=255)
                                   cgcTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACa                                                                                                               <  1:8136482/150‑1 (MQ=255)
                                     cTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAAt                                                                                                             <  2:550754/150‑1 (MQ=255)
                                     cTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAAt                                                                                                             >  2:9069515/1‑150 (MQ=255)
                                            ccAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGACGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGt                                                                                                      <  2:5259189/150‑1 (MQ=255)
                                            ccAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGt                                                                                                      <  2:2568645/150‑1 (MQ=255)
                                            ccAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGt                                                                                                      <  2:4106656/150‑1 (MQ=255)
                                            ccAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGt                                                                                                      <  1:10281094/150‑1 (MQ=255)
                                              aaGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGttt                                                                                                    <  1:3902245/150‑1 (MQ=255)
                                              aaGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGttt                                                                                                    <  1:10402729/150‑1 (MQ=255)
                                                 cTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCt                                                                                                 <  2:1266044/150‑1 (MQ=255)
                                                 cTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCt                                                                                                 <  1:6318611/150‑1 (MQ=255)
                                                     gcgcgGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAg                                                                                             >  1:9998685/1‑150 (MQ=255)
                                                      cgcgGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGa                                                                                            <  1:406210/150‑1 (MQ=255)
                                                      cgcgGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGa                                                                                            <  2:700563/150‑1 (MQ=255)
                                                       gcgGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGAc                                                                                           <  2:7663936/150‑1 (MQ=255)
                                                       gcgGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGAc                                                                                           <  1:8776352/150‑1 (MQ=255)
                                                        cgGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGCTAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACg                                                                                          <  2:1985383/150‑1 (MQ=255)
                                                        cgGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACg                                                                                          <  1:1728650/150‑1 (MQ=255)
                                                          gCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGgc                                                                                        >  2:5752171/1‑150 (MQ=255)
                                                          gCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGgc                                                                                        >  2:4396910/1‑150 (MQ=255)
                                                           ccGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGgcg                                                                                       <  2:879790/150‑1 (MQ=255)
                                                             gggATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCt                                                                                     >  1:7121689/1‑150 (MQ=255)
                                                             gggATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCt                                                                                     <  1:5151085/150‑1 (MQ=255)
                                                              ggATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTg                                                                                    >  2:5382059/1‑150 (MQ=255)
                                                               gATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGt                                                                                   <  2:4265007/150‑1 (MQ=255)
                                                               gATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGt                                                                                   <  1:4522027/150‑1 (MQ=255)
                                                                aTCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTc                                                                                  >  2:7368878/1‑150 (MQ=255)
                                                                aTCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTc                                                                                  <  1:5582013/150‑1 (MQ=255)
                                                                 tCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCg                                                                                 <  1:1287505/150‑1 (MQ=255)
                                                                  cAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGt                                                                                <  1:8238911/150‑1 (MQ=255)
                                                                   aaGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGtt                                                                               >  2:4456854/1‑150 (MQ=255)
                                                                    aGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGttt                                                                              >  1:2250639/1‑150 (MQ=255)
                                                                    aGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGttt                                                                              <  2:602232/150‑1 (MQ=255)
                                                                     ggCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTc                                                                             <  1:7746702/150‑1 (MQ=255)
                                                                      gCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTcc                                                                            <  1:9684065/150‑1 (MQ=255)
                                                                        cTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACACTGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGa                                                                          >  2:8911230/1‑150 (MQ=255)
                                                                        cTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGa                                                                          >  1:5465119/1‑150 (MQ=255)
                                                                         tCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGAc                                                                         <  1:4853811/150‑1 (MQ=255)
                                                                              cAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGc                                                                    >  2:3233349/1‑150 (MQ=255)
                                                                               aaCAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGCTAGAGCGACCATGGAAGGTTTCCTGCAGACGGCGCTGGCGTTTCCGACCGTGCt                                                                   >  2:8500081/1‑150 (MQ=255)
                                                                                aCAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTg                                                                  >  1:10202472/1‑150 (MQ=255)
                                                                                aCAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTg                                                                  <  1:8519815/150‑1 (MQ=255)
                                                                                   gCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTc                                                               >  1:9856851/1‑150 (MQ=255)
                                                                                    cGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACACTGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCa                                                              <  2:3901568/150‑1 (MQ=255)
                                                                                     gTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAg                                                             >  2:4770803/1‑150 (MQ=255)
                                                                                      tGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGc                                                            >  1:364132/1‑150 (MQ=255)
                                                                                        gATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCtt                                                          >  1:10634282/1‑150 (MQ=255)
                                                                                          tGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACACTGGAAGGTGTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTttc                                                        <  2:1620574/150‑1 (MQ=255)
                                                                                          tGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGATTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTttc                                                        <  1:4910999/150‑1 (MQ=255)
                                                                                           ggCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTttct                                                       >  2:94777/1‑150 (MQ=255)
                                                                                           ggCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTttct                                                       <  1:6198754/150‑1 (MQ=255)
                                                                                              gTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGCCGGCGTCCGGGCCCGTTTTTCAGGGAGAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGCCCGTGCTGTTCAGCTTtcttct                                                    >  2:6483273/1‑150 (MQ=255)
                                                                                               tGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTg                                                   >  2:7868720/1‑150 (MQ=255)
                                                                                                 cTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGAt                                                 >  1:99357/1‑150 (MQ=255)
                                                                                                      gcgcCTGAAGGAATAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATTGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTcg                                            <  1:7918138/150‑1 (MQ=255)
                                                                                                       cgcCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTcgc                                           <  2:5016341/150‑1 (MQ=255)
                                                                                                         ccTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTCGCGa                                         <  1:6401210/150‑1 (MQ=255)
                                                                                                         ccTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTCGCGa                                         <  1:7488080/150‑1 (MQ=255)
                                                                                                            gAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTCGCGatca                                      <  1:4438483/150‑1 (MQ=255)
                                                                                                              aGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTCGCGatcatc                                    <  1:4336864/150‑1 (MQ=255)
                                                                                                                  aaaGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTCGCGATCATCTACt                                >  1:1101309/1‑150 (MQ=255)
                                                                                                                         aaGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTCGCGATCATCTACTGGGGCAt                         <  2:8269960/150‑1 (MQ=255)
                                                                                                                         aaGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTCGCGATCATCGACTGGGGCAt                         >  1:1461218/1‑150 (MQ=255)
                                                                                                                             ccTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTCGCGATCATCTACTGGGGCAtcgtc                     <  2:10693354/150‑1 (MQ=255)
                                                                                                                              cTGATAGGCACGCGGCACGGCGAAGGAGTCCGGGCCCGTTATTCAGTGAAAGAGCGACAATGGACGGTTTCCTGCAGACGGCGCTGTCGTTTCAGACCGTGCTGTTAAGATTTCTTATGATCATCGCGATCATATACTTGGGCAtcgtcg                    >  2:10117514/1‑150 (MQ=255)
                                                                                                                              cTGAGAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGATTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTCGCGATCATCTACTGGGGCAtcgtcg                    <  2:3096028/150‑1 (MQ=255)
                                                                                                                                gATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTCGCGATCATCTACTGGGGCATCGTCGcc                  >  2:2864688/1‑150 (MQ=255)
                                                                                                                                 aaaGGACCGCGGCCCGGCGAAGGCGAAAGGGCCCGTTTTTCAGGTATAGAGCGACACTGGAAGGTTTCCTGCAGAAGGCGCTGTCGTTTCCGACCGTGCTGTTCACCTTACTTCTGATCCACGCGATCAACTACAGGGGCATCGTCGcca                 >  2:5433247/3‑149 (MQ=255)
                                                                                                                                 aTAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACACTGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTCGCGATCATCTACTGGGGCATCGTCGccc                 <  2:4629927/150‑1 (MQ=255)
                                                                                                                                 aTAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTCGCGATCATCTACTGGGGCATCGTCGccc                 <  1:10476097/150‑1 (MQ=255)
                                                                                                                                    ggCCCGCGGCCCGGCGCAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCGGTTGAGCTTTCTTATGAGCCGCGCGATAAGCGACGGGGGCATCGTCGCCCTgg              >  1:2877660/1‑150 (MQ=255)
                                                                                                                                        cgcgGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTCGCGATCCTCTACTGGGGCATCGTCGCCCTGGGCAt          >  1:9098661/1‑150 (MQ=255)
                                                                                                                                        cgcgGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTCGCGATCATCTACTGGGGCATCGTCGCCCTGGGCAt          >  2:1469015/1‑150 (MQ=255)
                                                                                                                                        cgcgGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTCGCGATCATCTACTGGGGCATCGTCGCCCTGGGCAt          <  1:3528513/150‑1 (MQ=255)
                                                                                                                                        cgcgGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCGCGCGATCATCTACTGGGGCATCGTCGCCCTGGGCAt          >  1:9559248/1‑150 (MQ=255)
                                                                                                                                            gCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTCGCGATCATCTACTGGGGCATCGTCGCCCTGGGCATGGTc      <  2:10283932/150‑1 (MQ=255)
                                                                                                                                            gCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTCGCGATCATCTACTGGGGCATCGTCGCCCTGGGCATGGTc      >  1:2731284/1‑150 (MQ=255)
                                                                                                                                             cccGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTCGCGATCATCTACTGGGGCATCGTCGCCCTGGGCATGGTCg     >  2:3792923/1‑150 (MQ=255)
                                                                                                                                              ccGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTCGCGATCATCTACTTGGGCAACGTCGCCCTGGGCATGGTCga    >  2:8356977/1‑150 (MQ=255)
                                                                                                                                              ccGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTCGCGATCATCTACTGGGGCATCGTCGCCCTGGGCATGGTCga    <  2:8124309/150‑1 (MQ=255)
                                                                                                                                                ggCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTCGCGATCATCTACTGGGGCATCGTCGCCCTGGGCATGGTCgaga  >  1:8096396/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GGCCGAACTGGCCAGCGCGGCGGCGCCGGACGATGCGCTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCAAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTCGCGATCATCTACTGGGGCATCGTCGCCCTGGGCATGGTCGAGA  >  NC_008463/1375800‑1376093

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑