Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 1,558,205 A→G Q390Q (CAA→CAG)  PA14_RS07290 → propionyl‑CoA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084631,558,2050AG99.8% 1874.2 / NA 474Q390Q (CAA→CAG) PA14_RS07290propionyl‑CoA synthetase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (250/223);  minor base T (0/1);  total (250/224)
Fisher's exact test for biased strand distribution p-value = 4.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 478 total aligned reads displayed.

CAAGGAAGACCCGCATGGCGAACGGGTGAAGCGCTACGACCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAAACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTGCTGGACGACGAAGGCCGGCCGCTGGGAGCGAACCGCCAGGG  >  NC_008463/1558058‑1558351
                                                                                                                                                   |                                                                                                                                                  
cAAGGAAGACCCGCATGGCGAACGGGTGAAGCGCTACGACCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGAc                                                                                                                                                  <  1:4469001/150‑1 (MQ=255)
cAAGGAAGACCCGCATGGCGAACGGGTGAAGCGCTACGACCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGAc                                                                                                                                                  <  1:3877351/150‑1 (MQ=255)
 aaGGAAGACCCGCATGGCGAACGGGTGAAGCGCTACGACCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGAcc                                                                                                                                                 >  1:10471702/1‑150 (MQ=255)
 aaGGAAGACCCGCATGGCGAACGGGTGAAGCGCTACGACCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCAGTGGTGGCAGAcc                                                                                                                                                 >  2:765400/1‑150 (MQ=255)
  aGGAAGACCCGCATGGCGAACGGGTGAAGCGCTACGACCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCg                                                                                                                                                >  2:9405749/1‑150 (MQ=255)
     aaGACCCGCATGGCGAACGGGTGAAGCGCTACGACCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCgaga                                                                                                                                             <  1:8646932/150‑1 (MQ=255)
      aGACCCGCATGGCGAACGGGTGAAGCGCTACGACCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGTGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGAc                                                                                                                                            >  1:10012160/1‑150 (MQ=255)
       gACCCGCATGGCGAACGGGTGAAGCGCTACGACCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGAcc                                                                                                                                           >  2:7826660/1‑150 (MQ=255)
        aCCCGCATGGCGAACGGGTGAAGCGCTACGACCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCagaccgagaccg                                                                                                                                          >  1:425167/1‑150 (MQ=255)
           cGCATGGCGAACGGGTGAAGCGCTACGCCCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCt                                                                                                                                       >  2:9237336/1‑150 (MQ=255)
            gCATGGCGAACGGGTGAAGCGCTACGACCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTg                                                                                                                                      <  2:5903280/150‑1 (MQ=255)
              aTGGCGAACGGGTGAAGCGCTACGACCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGc                                                                                                                                    <  1:1427324/150‑1 (MQ=255)
              aTGGCGAACGGGTGAAGCGCTACGACCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGc                                                                                                                                    >  2:3761862/1‑150 (MQ=255)
              aTGGCGAACGGGTGAAGCGCTACGACCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGc                                                                                                                                    <  2:3933224/150‑1 (MQ=255)
               tGGCGAACGGGTGAAGCGCTACGACCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGcc                                                                                                                                   <  1:5325799/150‑1 (MQ=255)
                 gCGAACGGGTGAAGCGCTACGACCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCgg                                                                                                                                 <  1:6441502/150‑1 (MQ=255)
                    aaCGGGTGAAGCGCTACGACCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGa                                                                                                                              >  2:5007541/1‑150 (MQ=255)
                    aaCGGGTGAAGCGCTACGACCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGa                                                                                                                              >  2:5505435/1‑150 (MQ=255)
                      cGGGTGAAGCTCTACGACCTAATTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGAcc                                                                                                                            <  1:951988/150‑1 (MQ=255)
                       gggTGAAGCGCTACGACCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACcg                                                                                                                           >  2:6299782/1‑150 (MQ=255)
                        ggTGAAGCGCTACGACCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACcgc                                                                                                                          >  2:10697426/1‑150 (MQ=255)
                        ggTGAAGCGCTACGACCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACcgc                                                                                                                          >  1:7515283/1‑150 (MQ=255)
                        ggTGAAGCGCTACGACCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACcgc                                                                                                                          >  1:6012027/1‑150 (MQ=255)
                        ggTGAAGCGCTACGACCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACcgc                                                                                                                          >  2:2606569/1‑150 (MQ=255)
                         gTGAAGCGCTACGACCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACcgcg                                                                                                                         >  2:9609073/1‑150 (MQ=255)
                          tGAAGCGCTACGACCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCgcgc                                                                                                                        >  2:5618326/1‑150 (MQ=255)
                             aGCGCTACGACCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCt                                                                                                                     <  2:8178603/150‑1 (MQ=255)
                              gcgcTACGACCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTg                                                                                                                    >  2:2109338/1‑150 (MQ=255)
                                   aCGACCTTAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCg                                                                                                               <  2:4214551/150‑1 (MQ=255)
                                   aCGACCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCg                                                                                                               >  1:4382400/1‑150 (MQ=255)
                                        cTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATc                                                                                                          >  1:2503295/1‑150 (MQ=255)
                                          aaGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGACGCTCGACAGCAGCACCCAGCACTGGCGGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCTACGGGATCGc                                                                                                        >  2:3090376/1‑150 (MQ=255)
                                          aaGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGc                                                                                                        >  1:5061970/1‑150 (MQ=255)
                                          aaGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGc                                                                                                        <  2:5140773/150‑1 (MQ=255)
                                          aaGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGc                                                                                                        >  2:9562672/1‑150 (MQ=255)
                                            gTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGCAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGGGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCg                                                                                                      <  1:153908/150‑1 (MQ=255)
                                            gTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCg                                                                                                      >  2:2976644/1‑150 (MQ=255)
                                                gCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCa                                                                                                  <  1:3369415/150‑1 (MQ=255)
                                                   gcgcCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGa                                                                                               >  1:2255574/1‑150 (MQ=255)
                                                      ccACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCt                                                                                            >  1:6101346/1‑150 (MQ=255)
                                                         ccTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCg                                                                                         >  2:1551491/1‑150 (MQ=255)
                                                         ccTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCg                                                                                         <  2:7253262/150‑1 (MQ=255)
                                                            gTTCCTGGCCGGCGAGACGCTCGCCAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCGGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGc                                                                                      <  2:6557700/150‑1 (MQ=255)
                                                            gTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGc                                                                                      <  2:6730098/150‑1 (MQ=255)
                                                                cTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGt                                                                                  <  1:4570014/150‑1 (MQ=255)
                                                                 tGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGtt                                                                                 <  2:6064710/150‑1 (MQ=255)
                                                                  ggCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTc                                                                                <  2:10062029/150‑1 (MQ=255)
                                                                     cGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGAc                                                                             <  2:1869701/150‑1 (MQ=255)
                                                                          agaAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAAcc                                                                        <  1:6786040/150‑1 (MQ=255)
                                                                          agaAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAAcc                                                                        >  1:8881312/1‑150 (MQ=255)
                                                                          agaAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAAcc                                                                        >  1:5712106/1‑150 (MQ=255)
                                                                          agaAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAAcc                                                                        >  1:7015995/1‑150 (MQ=255)
                                                                            aaGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACcgc                                                                      >  2:3644293/1‑150 (MQ=255)
                                                                             aGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACcgcg                                                                     <  2:2469702/150‑1 (MQ=255)
                                                                               cTCGAGAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCCCGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGTTTCGACCAACcgcgcg                                                                   <  2:4352544/150‑1 (MQ=255)
                                                                               cTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACcgcgcg                                                                   <  2:741211/150‑1 (MQ=255)
                                                                                tCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCgg                                                                  >  2:8276227/1‑150 (MQ=255)
                                                                                tCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCgg                                                                  <  1:6298661/150‑1 (MQ=255)
                                                                                tCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCgg                                                                  <  2:8667294/150‑1 (MQ=255)
                                                                                 cGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGt                                                                 <  2:2856168/150‑1 (MQ=255)
                                                                                    cagcagCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGcc                                                              <  2:6672303/150‑1 (MQ=255)
                                                                                     agcagcACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGccc                                                             <  1:4670178/150‑1 (MQ=255)
                                                                                      gcagcaCCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCg                                                            >  2:7020700/1‑150 (MQ=255)
                                                                                        agcaCCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGc                                                          <  2:1125990/150‑1 (MQ=255)
                                                                                        agcaCCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGc                                                          >  1:1999968/1‑150 (MQ=255)
                                                                                           aCCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTAc                                                       >  2:9041648/1‑150 (MQ=255)
                                                                                               aGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACg                                                   >  2:2266158/1‑150 (MQ=255)
                                                                                                gCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGt                                                  >  1:1163337/1‑150 (MQ=255)
                                                                                                  aCTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGc                                                >  2:4821850/1‑150 (MQ=255)
                                                                                                     ggctggAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAgg                                             <  1:10693791/150‑1 (MQ=255)
                                                                                                      gctggAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGt                                            <  1:7292604/150‑1 (MQ=255)
                                                                                                       ctggAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTg                                           <  1:1728818/150‑1 (MQ=255)
                                                                                                       ctggAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTg                                           <  1:7097149/150‑1 (MQ=255)
                                                                                                       ctggAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGGTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTg                                           >  2:10540314/1‑150 (MQ=255)
                                                                                                        tggAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGCCCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTGc                                          >  2:6814957/1‑150 (MQ=255)
                                                                                                        tggAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTGc                                          >  2:3267548/1‑150 (MQ=255)
                                                                                                         ggAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTGCt                                         >  1:8426150/1‑150 (MQ=255)
                                                                                                         ggAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTGCt                                         <  1:3196527/150‑1 (MQ=255)
                                                                                                         ggAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTGCt                                         <  1:8999572/150‑1 (MQ=255)
                                                                                                          gAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTGCTg                                        >  1:9567926/1‑150 (MQ=255)
                                                                                                             gagCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTGCTGgac                                     <  1:10312522/150‑1 (MQ=255)
                                                                                                               gCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTGCTGgacga                                   <  2:4619568/150‑1 (MQ=255)
                                                                                                               gCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTGCTGgacga                                   <  2:6423132/150‑1 (MQ=255)
                                                                                                                cacaCCGGGCGGCCGGTCCACGACCATTGGTGGCAGCCCGAGACCGGCGGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTGCTGgacgac                                  <  2:8010008/150‑1 (MQ=255)
                                                                                                                 acacCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTGCTGGacgacg                                 <  2:4103280/150‑1 (MQ=255)
                                                                                                                  cacCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTGCTGGAcgacga                                >  1:3573486/1‑150 (MQ=255)
                                                                                                                   acCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTGCTGGACGACGaa                               >  1:2692665/1‑150 (MQ=255)
                                                                                                                       ggcggcCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTGCTGGACGACGAAggcc                           <  1:7787584/150‑1 (MQ=255)
                                                                                                                             cGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTGCTGGACGACGAAGGCCGGCCGc                     >  1:10552185/1‑150 (MQ=255)
                                                                                                                              ggTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTGCTGGACGACGAAGGCCGGCCGCt                    <  1:4323401/150‑1 (MQ=255)
                                                                                                                               gTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCTTCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTGCTGGACGACGAAGGCCGGCCGCTg                   >  1:8811122/1‑150 (MQ=255)
                                                                                                                                tCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTGCTGGACGACGAAGGCCGGCCGCTgg                  <  2:9011599/150‑1 (MQ=255)
                                                                                                                                   aCGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTGCTGGACGACGAAGGCCGGCCGCTGGGAg               >  2:1595812/1‑150 (MQ=255)
                                                                                                                                    cGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTGCTGGACGACGAAGGCCGGCCGCTGGGAGc              <  1:5540856/150‑1 (MQ=255)
                                                                                                                                    cGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTGCTGGACGACGAAGGCCGGCCGCTGGGAGc              >  1:1358716/1‑150 (MQ=255)
                                                                                                                                        cATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTGCTGGACGACGAAGGCCGGCCGCTGGGAGCGAAc          <  1:2977235/150‑1 (MQ=255)
                                                                                                                                           tggtggCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTGCTGGACGACGAAGGCCGGCCGCTGGGAGCGAACCGc       <  1:9759562/150‑1 (MQ=255)
                                                                                                                                            ggtggCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTGCTGGACGACGAAGGCCGGCCGCTGGGAGCGAACCGcc      >  1:9310092/1‑150 (MQ=255)
                                                                                                                                               ggCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTGCTGGACGACGAAGGCCGGCCGCTGGGAGCGAACCGCCAgg   >  2:9790672/1‑150 (MQ=255)
                                                                                                                                                gCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTGCTGGACGACGAAGGCCGGCCGCTGGGAGCGAACCGCCAggg  >  2:1313853/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CAAGGAAGACCCGCATGGCGAACGGGTGAAGCGCTACGACCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAAACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTGCTGGACGACGAAGGCCGGCCGCTGGGAGCGAACCGCCAGGG  >  NC_008463/1558058‑1558351

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑