Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 2,589,402 C→T intergenic (‑45/+1) nuoK ← / ← nuoJ NADH‑quinone oxidoreductase subunit NuoK/NADH‑quinone oxidoreductase subunit J

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084632,589,4020CT96.8% 1608.0 / NA 471intergenic (‑45/+1)nuoK/nuoJNADH‑quinone oxidoreductase subunit NuoK/NADH‑quinone oxidoreductase subunit J
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (262/187);  minor base G (15/0);  total (284/187)
Fisher's exact test for biased strand distribution p-value = 6.20e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 473 total aligned reads displayed.

TCAGTACGAACAGGATGTTGCGTCGCACCATCAAGCCGACCAGTCCGAGGGCGAACAGGACGCTGGCGAGGGCCAGGCCGTGTTCCAGTGGTATTGCGTTCATAGCGTTACGTTCCCTTGGGGCGGGCGCAGGGCGCCCGCCGAACCCTTATTGTTTGGCGTCTTGACGACCCAGGTGATAGGCAGCGACCAGGGCCGCGAGCAACAGCATGGAGGCCAGTTCGACCACCAGCAGGTACGGGCCGTAGAGGCTGATGCCGACCGCCTTGGCGTCTACCGTGGTATGGCCGATGC  >  NC_008463/2589255‑2589548
                                                                                                                                                   |                                                                                                                                                  
 caGTACGAACAGGATGTTGCGTCGCACCATCAAGCCGACCAGTCCGAGGGCGAACAGGACGCTGGCGAGGGCCAGGCCGTGTTCCAGTGGTATTGCGTTCATAGCGTTACGTTCCCTTGGGGCGGGCGCAGGGCGCCCGCCGAACCTTTa                                                                                                                                                 <  1:9392645/150‑1 (MQ=255)
 caGTACGAACAGGATGTTGCGTCGCACCATCAAGCCGACCAGTCCGAGGGCGAACAGGACGCTGGCGAGGGCCAGGCCGTGTTCCAGTGGTATTGCGTTCATAGCGTTACGTTCCCTTGGGGCGGGCGCAGGGCGCCCGCCGAACCGTTa                                                                                                                                                 >  1:5108126/1‑150 (MQ=255)
   gtcctaaCCGGAGGTTGCGTCGCACCATCAACCCGACCCGTCGGTGGGCGAACCGGCCGCGGGCGAGGGCCAGGCGGTGTTCCTGTGGTTTGGCGTTCATAGCTTTCCGTTCCCTTGGGGCGGGCGCAGGGCGCCCGCCGAACCTTTAtt                                                                                                                                               <  1:4508921/145‑1 (MQ=255)
   gTACGAACAGGATGTTGCGTCGCACCATCAAGCCGACCAGTCCGAGGGCGAACAGGACGCTGGCGAGGGCCAGGCCGTGTTCCAGTGGTATTGCGTTCATAGCGTTACGTTCCCTTGGGGCGGGCGCAGGGCGCCCGCCGAACCTGTAtt                                                                                                                                               >  1:7811273/1‑150 (MQ=255)
    tACGAACAGGATGTTGCGTCGCACCATCAAGCCGACCAGTCCGAGGGCGAACAGGACGCTGGCGAGGGCCAGGCCGTGTTCCAGTGGTATTGCGTTCATAGCGTTACGTTCCCTTGGGGCGGGCGCAGGGCGCCCGCCGAACCTTTATTg                                                                                                                                              >  1:1953696/1‑150 (MQ=255)
     aCGAACAGGATGTTGCGTCGCACCATCAAGCCGACCAGTCCGAGGGCGAACAGGACGCTGGCGAGGGCCAGGCCGTGTTCCAGTGGTATTGCGTTCATAGCGTTACGTTCCCTTGGGGCGGGCGCAGGGCGCCCGCCGAACCTTTATTGt                                                                                                                                             >  1:8644076/1‑150 (MQ=255)
     aCGAACAGGATGTTGCGTCGCACCATCAAGCCGACCAGTCCGAGGGCGAACAGGACGCTGGCGAGGGCCAGGCCGTGTTCCAGTGGTATTGCGTTCATAGCGTTACGTTCCCTTGGGGCGGGCGCAGGGCGCCCGCCGAACCTTTATTGt                                                                                                                                             >  2:2080462/1‑150 (MQ=255)
        aaCAGGATGTTGCGTCGCACCATCAAGCCGACCAGTCCGAGGGCGAACAGGACGCTGGCGAGGGCCAGGCCGTGTTCCAGTGGTATTGCGTTCATAGCGTTACGTTCCCTTGGGGCGGGCGCAGGGCGCCCGCCGAACCTTTATTGTTTg                                                                                                                                          >  1:2796931/1‑150 (MQ=255)
         aCAGGATGTTGCGTCGCACCATCAAGCCGACCAGTCCGAGGGCGAACAGGACGCTGGCGAGGGCCAGGCCGTGTTCCAGTGGTATTGCGTTCATAGCGTTACGTTCCCTTGGGGCGGGCGCAGGGCGCCCGCCGAACCTTTATTGTTTgg                                                                                                                                         >  2:564633/1‑150 (MQ=255)
          cAGGATGTTGCGTCGCACCATCAAGCCGACCAGTCCGAGGGCGAACAGGACGCTGGCGAGGGCCAGGCCGTGTTCCAGTGGTATTGCGTTCATAGCGTTACGTTCCCTTGGGGCGGGCGCAGGGCGCCCGCCGAACCTTTATTGTTTGGc                                                                                                                                        <  1:5785483/150‑1 (MQ=255)
           aGGATGTTGCGTCGCACCATCAAGCCGACCAGTCCGAGGGCGAACAGGACGCTGGCGAGGGCCAGGCCGTGTTCCAGTGGTATTGCGTTCATAGCGTTACGTTCCCTTGGGGCGGGCGCAGGGCGCCCGCCGAACCTTTATTGTTTGGCg                                                                                                                                       <  2:5108126/150‑1 (MQ=255)
              aTGTTGCGTCGCACCATCAAGCCGACCTGTCCGAGGGCGAACAGGACGCTGGCGAGGGCCAGGCCGTGTTCCAGTGGTATTGCGTTCATAGCGTTACGTTCCCTTGGGGCGGGCGCAGGGCGCCCGCCGAACCTTTATTGTTTGGCGTCt                                                                                                                                    <  1:329244/150‑1 (MQ=255)
              aTGTTGCGTCGCACCATCAAGCCGACCAGTCCGAGGGCGAACAGGACGCTGGCGAGGGCCAGGCCGTGTTCCAGTGGTATTGCGTTCATAGCGTTACGTTCCCTTGGGGCGGGCGCAGGGCGCCCGCCGAACCGTTAGTGTTTGGCGTCt                                                                                                                                    >  2:4681020/1‑150 (MQ=255)
                gTTGCGTCGCACCATCAAGCCGACCAGTCCGAGGGCGAACAGGACGCTGGCGAGGGCCAGGCCGTGTTCCAGTGGTATTGCGTTCATAGCGTTACGTTCCCTTGGGGCGGGCGCAGGGCGCCCGCCGAACCTTTATTGTTTGGCGTCTTg                                                                                                                                  <  1:6435533/150‑1 (MQ=255)
                 ttGCGTCGCACCATCAAGCCGACCAGTCCGAGGGCGAACAGGACGCTGGCGAGGGCCAGGCCGTGTTCCAGTGGTATTGCGTTCATAGCGTTACGTTCCCTTGGGGCGGGCGCTGGGCGCCCGCCGAACCTTTATTGTTTGGCGTCTTga                                                                                                                                 <  2:8645385/150‑1 (MQ=255)
                 ttGCGTCGCACCATCAAGCCGACCAGTCCGAGGGCGAACAGGACGCTGGCGAGGGCCAGGCCGTGTTCCAGTGGTAGTGCGTTCATAGCGTTACGTTCCCTTGGGGCGGGCGCAGGGCGCCCGCCGAACCTGTATTGTTTGGCGTCTTga                                                                                                                                 >  2:3937439/1‑150 (MQ=255)
                  tGCGTCGCACCATCAAGCCGACCAGTCCGAGGGCGAACAGGACGCTGGCGAGGGCCAGGCCGTGTTCCAGTGGTATTGCGTTCATAGCGTTACGTTCCCTTGGGGCGGGCGCAGGGCGCCCGCCGAACCTTTATTGTTTGGCGTCTTgac                                                                                                                                <  2:10617325/150‑1 (MQ=255)
                   gCGTCGCACCATCAAGCCGACCAGTCCGAGGGCGAACAGGACGCTGGCGAGGGCCAGGCCGTGTTCCAGTGGTATTGCGTTCATAGCGTTACGTTCCCTTGGGGCGGGCGCAGGGCGCCCGCCGAACCTTTATTGTTTGGCGTCTTgacg                                                                                                                               >  2:2996453/1‑150 (MQ=255)
                    cGTCGCACCATCAAGCCGACCAGTCCGAGGGCGAACAGGACGCTGGCGAGGGCCAGGCCGTGTTCCAGTGGTATTGCGTTCATAGCGTTACGTTCCCTTGGGGCGGGCGCAGGGCGCCCGCCGAACCTTTATTGTTTGGCGTCTTgacga                                                                                                                              >  1:5270851/1‑150 (MQ=255)
                     gTCGCACCATCAAGCCGACCAGTCCGAGGGCGAACAGGACGCTGGCGAGGGCCAGGCCGTGTTCCAGTGGTATTGCGTTCATAGCGTTACGTTCCCTTGGGGCGGGCGCAGGGCGCCCGCCGACCCTTGAGTGTTTGGCGTCTTgacgac                                                                                                                             >  1:3495891/1‑150 (MQ=255)
                        gCACCATCCCGCCCACCAGTCCGTGGGCGAACAGGTCGCGCTGGAGGGCCAGGCCGTGTTCCAGTGGTATTGCGTTCATAGCGTTACGTTCCCTTGGGGCGGGCGCAGGGCGCCCGCCGAACCTTTATTGTTTGGCGTCTTGACGACCCa                                                                                                                          <  2:5456309/150‑1 (MQ=255)
                        gCACCATCAAGCCGACCAGTCCGAGGGCGAACAGGACGCTGGCGAGGGCCAGGCCGTGTTCCAGTGGTATTGCGTTCATAGCGTTACGTTCCCTTGGGGCGGGCGCAGGGCGCCCGCCGAACCTTTATTGTTTGGCGTCTTGACGACCCa                                                                                                                          >  2:2251448/1‑150 (MQ=255)
                        gCACCATCAAGCCGACCAGTCCGAGGGCGAACAGGACGCTGGCGAGGGCCAGGCCGTGTTCCAGTGGTATTGCGTTCATAGCGTTACGTTCCCTTGGGGCGGGCGCAGGGCGCCCGCCGAACCTTTATTGTTTGGCGTCTTGACGACCCa                                                                                                                          <  1:8794958/150‑1 (MQ=255)
                            cATCAAGCCGACCAGTCCGAGGGCGAACAGGACGCTGGCGAGGGCCAGGCCGTGTTCCAGTGGTATTGCGTTCATAGCGTTACGTTCCCTTGGGGCGGGCGCAGGGCGCCCGCCGAACCTTTATTGTTTGGCGTCTTGACGACCCAGGTg                                                                                                                      >  2:405845/1‑150 (MQ=255)
                             aTCAAGCCGACCAGTCCGAGGGCGAACAGGACGCTGGCGAGGGCCAGGCCGTGTTCCAGTGGTATTGCGTTCATAGCGTTACGTTCCCTTGGGGCGGGCGCAGGGCGCCCGCCGAACCTTTATTGTTTGGCGTCTTGACGACCCAGGTGa                                                                                                                     <  1:9748393/150‑1 (MQ=255)
                             aTCAAGCCGACCAGTCCGAGGGCGAACAGGACGCTGGCGAGGGCCAGGCCGTGTTCCAGTGGTATTGCGTTCATAGCGTTACGTTCCCTTGGGGCGGGCGCAGGGCGCCCGCCGAACCTTTATTGTTTGGCGTCTTGACGACCCAGGTGa                                                                                                                     >  2:2668661/1‑150 (MQ=255)
                               cAAGCCGACCAGTCCGAGGGCGAACAGGACGCTGGCGAGGGCCAGGCCGTGTTCCAGTGGTATTGCGTTCATAGCGTTACGTTCCCTTGGGGCGGGCGCAGGGCGCCCGCCGAACCTTTATTGTTTGGCGTCTTGACGACCCAGGTGATa                                                                                                                   <  1:2305602/150‑1 (MQ=255)
                                 aGCCGACCAGTCCGAGGGCGAACAGGACGCTGGCGAGGGCCAGGCCGTGTTCCAGTGGTATTGCGTTCATAGCGTTACGTTCCCTTGGGGCGGGCGCAGGGCGCCCGCCGAACCTTTATTGTTTGGCGTCTTGACGACCCAGGTGATAgg                                                                                                                 <  1:909057/150‑1 (MQ=255)
                                      aCCAGTCCGAGGGCGAACCGGACGCTGGCGAGGGCCAGGCCGTGTTCCAGTGGTATTGCGTTCATAGCGTTCCGTGCCCTTGGGGCGGGCGCAGGGCGCCCGCCGAACCTTTATTGTTTGGCGTCTTGACGACCCAGGTGATAGGCAGCg                                                                                                            <  1:4144368/150‑1 (MQ=255)
                                      aCCAGTCCGAGGGCGAACAGGACGCTGGCGAGGGCCAGGCCGTGTTCCAGTGGTATTGCGTTCATAGCGTTACGTTCCCTTGGGGCGGGCGCAGGGCGCCCGCCGAACCTTTATTGTTTGGCGTCTTGACGACCCAGGTGATAGGCAGCg                                                                                                            <  1:8182533/150‑1 (MQ=255)
                                         aGTCCGAGGGCGAACAGGACGCTGGCGAGGGCCAGGCCGTGTTCCAGTGGTATTGCGTTCATAGCGTTACGTTCCCTTGGGGCGGGCGCAGGGCGCCCGCCGAACCTTTATTGTTTGGCGTCTTGACGACCCAGGTGATAGGCAGCGAcc                                                                                                         >  1:6819679/1‑150 (MQ=255)
                                          gTCCGAGGGCGAACAGGACGCTGGCGAGGGCCAGGCCGTGTTCCAGTGGTATTGCGTTCATAGCGTTACGTTCCCTTGGGGCGGGCGCAGGGCGCCCGCCGAACCTTTATTGTTTGGCGTCTTGACGACCCAGGTGATAGGCAGCGACCa                                                                                                        >  2:6948987/1‑150 (MQ=255)
                                          gTCCGAGGGCGAACAGGACGCTGGCGAGGGCCAGGCCGTGTTCCAGTGGTATTGCGTTCATAGCGTTACGTTCCCTTGGGGCGGGCGCAGGGCGCCCGCCGAACCTTTATTGTTTGGCGTCTTGACGACCCAGGTGATAGGCAGCGACCa                                                                                                        >  1:8395603/1‑150 (MQ=255)
                                               aGGGCGAACAGGACGCTGGCGAGGGCCAGGCCGTGTTCCAGTGGTATTGCGTTCATAGCGTTACGTTCCCTTGGGGCGGGCGCAGGGCGCCCGCCGAACCTTTATTGTTTGGCGTCTTGACGACCCAGGTGATAGGCAGCGACCAGGGcc                                                                                                   >  2:8452958/1‑150 (MQ=255)
                                               aGGGCGAACAGGACGCTGGCGAGGGCCAGGCCGTGTTCCAGTGGTATTGCGTTCATAGCGTTACGTTCCCTTGGGGCGGGCGCAGGGCGCCCGCCGAACCTTTATTGTTTGGCGTCTTGACGACCCAGGTGATAGGCAGCGACCAGGGcc                                                                                                   >  2:3098028/1‑150 (MQ=255)
                                               aGGGCGAACAGGACGCTGGCGAGGGCCAGGCCGTGTTCCAGTGGTATTGCGTTCATAGCGTTACGTTCCCTTGGGGCGGGCGCAGGGCGCCCGCCGAACCTGTATTGTTTGGCGTCTTGAAGACCCAGGTGATAGGCAGCGACCAGGGcc                                                                                                   >  2:9552401/1‑150 (MQ=255)
                                                gggCGAACAGGACGCTGGCGAGGGCCAGGCCGTGTTCCAGTGGTATTGCGTTCATAGCGTTACGTTCCCTTGGGGCGGGCGCAGGGCGCCCGCCGAACCTTTATTGTTTGGCGTCTTGACGACCCAGGTGATAGGCAGCGACCAGGGCcg                                                                                                  >  2:9370210/1‑150 (MQ=255)
                                                 ggCGAACAGGACGCTGGCGAGGGCCAGGCCGTGTTCCAGTGGTATTGCGTTCATAGCGTTACGTTCCCTTGGGGCGGGCGCAGGGCGCCCGCCGAACCTTTATTGTTTGGCGTCTTGACGACCCAGGTGATa                                                                                                                   >  2:3310341/1‑132 (MQ=255)
                                                 ggCGAACAGGACGCTGGCGAGGGCCAGGCCGTGTTCCAGTGGTATTGCGTTCATAGCGTTACGTTCCCTTGGGGCGGGCGCAGGGCGCCCGCCGAACCTGTATTGTTTGGCGTCTTGACGACCCAGGTGATCGGCAGCGACCAGGGCcgc                                                                                                 >  2:5245793/1‑150 (MQ=255)
                                                  gCGAACTGGACGCTGGCGAGGGCCTGGCCGTGTTCCAGTGGTATTGCGTTCATAGCGTTACGTTCCATTGGGGCGGGCGCAGGGCGCCCGCCGAACCTTTATTGTTTGGCGTCTTGACGACCCAGGTGATAGGCAGCGACCAGGGCcgcg                                                                                                <  1:4969074/150‑1 (MQ=255)
                                                  gCGAACAGGACGCTGGCGAGGGCCAGGCCGTGTTCCAGTGGTATTGCGTTCATAGCGTGACGTTCCCTTGGGGCGGGCGCAGGGCGCCCGCCGAACCTTTATTGTTTGGCGTCTTGACGACCCAGGTGATAGGCAGCGACCAGGGCcgcg                                                                                                >  2:10467397/1‑150 (MQ=255)
                                                    gAACAGGACGCTGGCGAGGGCCAGGCCGTGTTCCAGTGGTATTGCGTTCATAGCGTTACGTTCCCTTGGGGCGGGCGCAGGGCGCCCGCCGAACCTTTATTGTTTGGCGTCTTGACGACCCAGGTGATAGGCAGCGACCAGGGCCGCGAg                                                                                              >  1:2592644/1‑150 (MQ=255)
                                                     aaCAGGACGCTGGCGAGGGCCAGGCCGTGTTCCAGTGGTATTGCGTTCATAGCGTTACGTTCCCTTGGGGCGGGCGCAGGGCGCCCGCCGAACCTTTATTGTTTGGCGTCTTGACGACCCAGGTGATAGGCAGCGACCAGGGCCGCGAGc                                                                                             >  1:7715437/1‑150 (MQ=255)
                                                        aGGACGCTGGCGAGGGCCAGGCCGTGTTCCAGTGGTATTGCGTTCATAGCGTTACGTTCCCTTGGGGCGGGCGCAGGGCGCCCGCCGAACCTTTATTGTTTGGCGTCTTGACGACCCAGGTGATAGGCAGCGACGAGGGCCGCGAGCacc                                                                                          >  2:6438296/1‑148 (MQ=255)
                                                         ggACGCTGGCGAGGGCCAGGCCGTGTTCCAGTGGTATTGCGTTCATAGCGTTACGTTCCCTTGGGGCGGGCGCAGGGCGCCCGCCGAACCTTTATTGTTTGGCGTCTTGACGACCCAGGTGATAGGCAGCGACCAGGGCCGCGAGCAACa                                                                                         >  2:8720736/1‑150 (MQ=255)
                                                         ggACGCTGGCGAGGGCCAGGCCGTGTTCCAGTGGTATTGCGTACATAGCTTTACGTTCCCTTGGGGCGGGCGCAGGGCGCCCGCCGAACCTTTATTGTTTGGCGTCTTGACGACCCAGGTGATAGGCAGCGACCAGGGCCGCGAGCAACa                                                                                         <  1:1788513/150‑1 (MQ=255)
                                                           aCGCTGGCGAGGGCCAGGCCGTGTTCCAGTGGTATTGCGTTCATAGCGTTACGTTCCCTTGGGGCGGGCGCAGGGCGCCCGCCGAACCTTTATTGTTTGGCGTCTTGACGACCCAGGTGATAGGCAGCGACCAGGGCCGCGAGCAACAGc                                                                                       >  1:3030534/1‑150 (MQ=255)
                                                             gCTGGCGAGGGCCAGGCCGTGTTCCAGTGGTATTGCGTTCATAGCGTTACGTTCCCTTGGGGCGGGCGCAGGGCGCCCGCCGAACCTTTATTGTTTGGCGTCTTGACGACCCAGGTGATAGGCAGCGACCAGGGCCGCGAGCAACAGCAt                                                                                     <  2:1017122/150‑1 (MQ=255)
                                                              cTGGCGAGGGCCAGGCCGTGTTCCAGTGGTATTGCGTTCATAGCGTTACGTTCCCTTGGGGCGGGCGCAGGGCGCCCGCCGAACCTTTATTGTTTGGCGTCTTGACGACCCAGGTGATAGGCAGCGACCAGGGCCGCGAGCAACAGCATg                                                                                    <  1:7096343/150‑1 (MQ=255)
                                                                  cGAGGGCCAGGCCGTGTTCCAGTGGTATTGCGTTCATAGCGTTACGTTCCCTTGGGGCGGGCGCAGGGCGCCCGCCGAACCTTTATTGTTTGGCGTCTTGACGACCCAGGTGATAGGCAGCGACCAGGGCCGCGAGCAACAGCATGGAgg                                                                                <  2:3905157/150‑1 (MQ=255)
                                                                    aGGGCCAGGCCGTGTTCCAGTGGTATTGCGTTCATAGCGTTACGTTCCCTTGGGGCGGGCGCAGGGCGCCCGCCGAACCTTTATTGTTTGGCGTCTTGACGACCCAGGTGATAGGCAGCGTCCAGGGCCGCGAGCAACAGCATGGAGGcc                                                                              >  2:7554355/1‑150 (MQ=255)
                                                                        ccAGGCCGTGTTCCAGTGGTATTGCGTTCATAGCGTTACGTTCCCTTGGGGCGGGCGCAGGGCGCCCGCCGAACCTTTAGTGTTTGGCGTCTTGACGACCCAGGTGATAGGCAGCGACCAGGGCCGCGAGCAACAGCATGGAGGCCAGtt                                                                          <  2:8581319/150‑1 (MQ=255)
                                                                          aGGCCGTGTTCCAGTGGTATTGCGTTCATAGCGTTACGTTCCCTTGGGGCGGGCGCAGGGCGCCCGCCGAACCTTTATTGTTTGGCGTCTTGACGACCCAGGTGATAGGCAGCGACCAGGGCCGCGAGCAACAGCATGGAGGCCAGTTCg                                                                        <  1:6619352/150‑1 (MQ=255)
                                                                           ggCCGTGTTCCAGTGGTATTGCGTTCATAGCGTTACGTTCCCTTGGGGCGGGCGCAGGGCGCCCGCCGAACCTTTATTGTTTGGCGTCTTGACGACCCAGGTGATAGGCAGCGACCAGGGCCGCGAGCAACAGCATGGAGGCCAGTTCGa                                                                       >  2:9292908/1‑150 (MQ=255)
                                                                           ggCCGTGTTCCAGTGGTATTGCGTTCATAGCGTTACGTTCCCTTGGGGCGGGCGCAGGGCGCCCGCCGAACCTTTATTGTTTGGCGTCTTGACGACCCAGGTGATAGGCAGCGACCAGGGCCGCGAGCAACAGCATGGAGGCCAGTTCGa                                                                       >  1:6210138/1‑150 (MQ=255)
                                                                            gCCGTGTTCCAGTGGTATTGCGTTCATAGCGTTACGTTCCCTTGGGGCGGGCGCAGGGCGCCCGCCGAACCTTTATTGTTTGGCGTCTTGACGACCCAGGTGATAGGCAGCGACCAGGGCCGCGAGCAACAGCATGGAGGCCAGTTCGac                                                                      >  2:2754055/1‑150 (MQ=255)
                                                                                 gtTCCAGTGGTATTGCGTTCATAGCGTTACGTTCCCTTGGGGCGGGCGCAGGGCGCCCGCCGAACCTTTATTGTTTGGCGTCTTGACGACCCAGGTGATAGGCAGCGACCAGGGCCGCGAGCAACAGCATGGAGGCCAGTTCGAccacca                                                                 >  2:1594856/1‑150 (MQ=255)
                                                                                  ttCCAGTGGTATTGCGTTCATAGCGTTACGTTCCCTTGGGGCGGGCGCAGGGCGCCCGCCGAACCTTTATTGTTTGGCGTCTTGACGACCCAGGTGATAGGCAGCGACCAGGGCCGCGAGCAACAGCATGGAGGCCAGTTCGACCACcag                                                                <  1:6952969/150‑1 (MQ=255)
                                                                                    ccAGTGGTATTGCGTTCATAGCGTTACGTTCCCTTGGGGCGGGCGCAGGGCGCCCGCCGAACCTTTATTGTTTGGCGTCTTGACGACCCAGGTGATAGGCAGCGACCAGGGCCGCGAGCAACAGCATGGAGGCCAGTTCGACCACcagca                                                              >  1:9513089/1‑150 (MQ=255)
                                                                                     cAGTGGTATCGCGTTCCTAGCGTTCCGGTCCCTTGGGGCGGGCGCAGGGCGCCCGCCGAACCTTTATTGTTGGGCGTCTTGACGACCCAGGAGAGAGGCAGCGACCAGGGCCGCGAGCACCAGCATGGAGGCCAGTTCGACCACcagcag                                                             <  2:3673098/150‑1 (MQ=255)
                                                                                       gTGGTATTGCGTTCATAGCGTTACGTTCCCTTGGGGCGGGCGCAGGGCGCCCGCCGAACCTTTATTGTTTGGCGTCTTGACGACCCAGGTGATAGGCAGCGACCAGGGCCGCGAGCAACAGCATGGAGGCCAGTTCGACCACCAGCAGGt                                                           >  1:4670969/1‑150 (MQ=255)
                                                                                       gTGGTATTGCGTTCATAGCGTTACGTTCCCTTGGGGCGGGCGCAGGGCGCCCGCCGAACCTTTATTGTTTGGCGTCTTGACGACCCAGGTGATAGGCAGCGACCAGGGCCGCGAGCAACAGCATGGAGGCCAGTTCGACCACCAGCAGGt                                                           <  1:3690799/150‑1 (MQ=255)
                                                                                       gTGGTATTGCGTTCATAGCGTTACGTTCCCTTGGGGCGGGCGCAGGGCGCCCGCCGAACCATTATTGTTTGGCGTCTTGACGACCCAGGTGATAGGCAGCGACCAGGGCCGCGAGCAACAGCATGGAGGCCAGTTCGACCACCAGCAGGt                                                           >  1:7260575/1‑150 (MQ=255)
                                                                                          gTATTGCGTTCATAGCGTTACTTTCCCTTGGGGCGGGCGCAGGGCGCCCGCCGAACCTTTATTGTTTGGCGTCTTGACGACCCAGGTGATAGGCAGCGACCAGGGCCGCGAGCAACAGCATGGAGGCCAGTTCGACCACCAGCAGGTACg                                                        <  1:9250290/150‑1 (MQ=255)
                                                                                             ttGCGTTCATAGCGTTACGTTCCCTTGGGGCGGGCGCAGGGCGCCCGCCGAACCGTTATTGTTTGGCGTCTTGACGACCCAGGTGATAGGCAGCGACCAGGGCCGCGAGCAACAGCCTGGAGGCCAGTTCGACCACCAGCAGGTACGGGc                                                     >  1:3911742/1‑150 (MQ=255)
                                                                                               gCGTTCATAGCGTTACGTTCCCTTGGGGCGGGCGCAGGGCGCCCGCCGAACCTTTATTGTTTGGCGTCTTGACGACCCAGGTGATAGTCATCGACCAGGTCCGCGAGCAACAGCATGGAGGCCAGTTCGACCACCAGCAGGTACGGGCCg                                                   <  2:7081706/150‑1 (MQ=255)
                                                                                                  ttCATAGCGTTACGTTCCCTTGGGGCGGGCGCAGGGCGCCCGCCGAACCTTTATTGTTTGGCGTCTTGACGACCCAGGTGATAGGCAGCGACCAGGGCCGCGAGCACCAGCATGGAGGCCAGTTCGACCACCAGCAGGTACGGGCCGTag                                                >  2:435373/1‑150 (MQ=255)
                                                                                                  ttCATAGCGTTACGTTCCCTTGGGGCGGGCGCAGGGCGCCCGCCGAACCTTTATTGTTTGGCGTCTTGACGACCCAGGTGATAGGCAGCGACCAGGGCCGCGAGCAACAGCATGGAGGCCAGTTCGACCACCAGCAGGTACGGGCCGTag                                                >  2:9794749/1‑150 (MQ=255)
                                                                                                  ttCATAGCGTTACGTTCCCTTGGGGCGGGCGCAGGGCGCCCGCCGAACCGTTATTGTTTGGCGTCTTGACGACCCAGGTGATAGGCAGCGACCAGGGCCGCGAGCAACAGCATGGAGGCCAGTTCGACCACCAGCCGGTACGGGCCGTag                                                >  1:10294847/1‑150 (MQ=255)
                                                                                                        gCGTTACGTTCCCTTGGGGCGGGCGCAGGGCGCCCGCCGAACCTTTATTGTTTGGCGTCTTGACGACCCAGGTGATAGGCAGCGACCAGGGCCGCGAGCAACAGCATGGAGGCCAGTTCGACCACCAGCAGGTACGGGCCGTAGAGGCTg                                          >  2:3958691/1‑150 (MQ=255)
                                                                                                         cGTTACGTTCCCTTGGGGCGGGCGCAGGGCGCCCGCCGAACCTTTATTGTTTGGCGTCTTGACGACCCAGGTGATAGGCAGCGACCAGGGCCGCGAGCCACAGCATGGAGGCCAGTTCGACCACCAGCAGGTACGGGCCGTAGAGGCTGa                                         >  1:8541241/1‑150 (MQ=255)
                                                                                                         cGTTACGTTCCCTTGGGGCGGGCGCAGGGCGCCCGCCGAACCTTTATTGTTTGGCGTCTTGACGACCCAGGTGATAGGCAGCGACCAGGGCCGCGAGCAACAGCATGGAGGCCAGTTCGACCACCAGCAGGTACGGGCCGTAGAGGCTGa                                         >  1:1190581/1‑150 (MQ=255)
                                                                                                           ttACGTTCCCTTGGGGCGGGCGCAGGGCGCCCGCCGAACCTTTATTGTTTGGCGTCTTGACGACCCAGGTGATAGGCAGCGACCAGGGCCGCGAGCAACAGCATGGAGGCCAGTTCGACCACCAGCAGGTACGGGCCGTAGAGGCTGATg                                       <  2:4640197/150‑1 (MQ=255)
                                                                                                           ttACGTTCCCTTGGGGCGGGCGCAGGGCGCCCGCCGAACCGTTATTGTTTGGCGTCTTGACGACCCAGGTGATAGGCAGCGACCAGGGCCGCGAGCAACAGCATGGAGGCCAGTTCGACCACCAGCAGGTCCGGGCCGTAGAGGCTGATg                                       >  2:7310164/1‑150 (MQ=255)
                                                                                                            tACGTTCCCTTGGGGCGGGCGCAGGGCGCCCGCCGAACCGTTATTGTTTGGCGTCTTGACGACCCAGGTGAGAGGCAGCGACCAGGGCCGCGAGCAACAGCATGGAGGCCAGTTAGACCACCAGCAGGTACGGGCCGTAGAGGCTGATGc                                      >  1:2166708/1‑150 (MQ=255)
                                                                                                             aCGTTCCCTTGGGGCGGGCGCAGGGCGCCCGCCGAACCGTTATTTTTTGGCGTCTTGACGACCCAGGTGATAGGCAGCGACCAGGGCCGCGAGCAACAGCATGGAGGCCAGTTCGACCACCAGCAGGTACGGGCCGTAGAGGCTGATGcc                                     >  1:1152789/1‑150 (MQ=255)
                                                                                                               gTTCCCTTGGGGCGGGCGCAGGGCGCCCGCCGAACCTTTATTGTTTGGCGTCTTGACGACCCAGGTGATAGGCAGCGACCAGGGCCGCGAGCAACAGCATGGAGGCCAGTTCGACCACCAGCAGGTACGGGCCGTAGAGGCTGATGCCGa                                   >  2:10360998/1‑150 (MQ=255)
                                                                                                                ttCCCTTGGGGCGGGCGCAGGGCGCCCGCCGAACCTTTATTGTTTGGCGTCTTGACGACCCAGGTGATAGGCAGCGACCAGGGCCGCGAGCAACAGCATGGAGGCCAGTTCGACCACCAGCAGGTACGGGCCGTAGAGGCTGATGCCGAc                                  <  1:1008553/150‑1 (MQ=255)
                                                                                                                       ggggCGGGCGCAGGGCGCCCGCCGAACCTTTATTGTTTGGCGTCTTGACGACCCAGGTGATAGGCAGCGACCAGGGCCGCGAGCAACAGCATGGAGGCCAGTTCGACCACCAGCAGGTACGGGCCGTAGAGGCTGATGCCGACCGCCTTg                           >  2:503421/1‑150 (MQ=255)
                                                                                                                        gggcgggcGCAGGGCGCCCGCCGAACCTTTATTGTTTGGCGTCTTGACGACCCAGGTGATAGGCAGCGACCAGGGCCGCGAGCAACAGCATGGAGGCCAGTTCGACCACCAGCAGGTACGGGCCGTAGAGGCTGATGCCGACCGCCTTgg                          <  2:770207/150‑1 (MQ=255)
                                                                                                                           cgggcgCAGGGCGCCCGCCGAACCTTTATTGTTTGGCGTCTTGACGACCCAGGTGATAGGCAGCGACCAGGGCCGCGAGCAACAGCATGGAGGCCAGTTCGACCACCAGCAGGTACGGGCCGTAGAGGCTGATGCCGACCGCCTTGGCGt                       <  1:4289329/150‑1 (MQ=255)
                                                                                                                           cgggcgCAGGGCGCCCGCCGAACCTTTATTGTTTGGCGTCTTGACGACCCAGGTGATAGGCAGCGACCAGGGCCGCGAGCAACAGCATGGAGGCCAGTTCGACCACCAGCAGGTACGGGCCGTAGAGGCTGATGCCGACCGCCTTGGCGt                       <  2:6005339/150‑1 (MQ=255)
                                                                                                                             ggcgCAGGGCGCCCGCCGAACCTTTATTGTTTGGCGTCTTGACGACCCAGGTGATAGGCAGCGACCAGGGCCGCGAGCAACAGCATGGAGGCCAGTTCGACCACCAGCAGGTACGGGCCGTAGAGGCTGATGCCGACCGCCTTGGCGTCt                     <  2:9080184/150‑1 (MQ=255)
                                                                                                                             ggcgCAGGGCGCCCGCCGAACCGTTATTGTTTGGCGTCTTGACGACCCAGGTGATAGGCAGCGACCAGGGCCGCGAGCAACAGCATGGAGGCCAGTTCGACCACCAGCAGGTACGGGCCGTAGAGGCTGATGCCGACCGCCTTGGCGTCt                     >  1:5160651/1‑150 (MQ=255)
                                                                                                                              gcgcAGGGCGCCCGCCGAACCTTTATTGTTTGGCGTCTTGACGACCCAGGTGATAGGCAGCGACCAGGGCCGCGAGCAACAGCATGGAGGCCAGTTCGACCACCAGCAGGTACGGGCCGTAGAGGCTGATGCCGACGGCCTTGGCGTCTa                    <  1:5476346/150‑1 (MQ=255)
                                                                                                                                 cAGGGCGCCCGCCGAACCTTTATTGTTTGGCGTCTTGACGACCCAGGTGATAGGCAGCGACCAGGGCCGCGAGCAACAGCATGGAGGCCAGTTCGACCACCAGCAGGTACGGGCCGGAGAGGCTGATGCCGACCGCCTTGGCGTCTACCg                 >  2:1515282/1‑150 (MQ=255)
                                                                                                                                   gggCGCCCGCCGAACCTTTATTGTTTGGCGTCTTGACGACCCAGGTGATAGGCAGCGACCAGGGCCGCGAGCAACAGCATGGAGGCCAGTTCGACCACCAGCAGGTACGGGCCGTAGAGGCTGATGCCGACCGCCTTGGCGTCTACCGTg               >  2:3475574/1‑150 (MQ=255)
                                                                                                                                   gggCGCCCGCCGAACCTTTATTGTTTGGCGTCTTGACGACCCAGGTGATAGGCAGCGACCAGGGCCGCGAGCAACAGCATGGAGGCCAGTTCGACCACCAGCAGGTACGGGCCGTAGAGGCTGATGCCGACCGCCTTGGCGTCTACCGTg               >  1:9898254/1‑150 (MQ=255)
                                                                                                                                       gcccgccGAACCTTTATTGTTTGGCGTCTTGACGACCCAGGTGATAGGCAGCGACCAGGGCCGCGAGCAACAGCATGGAGGCCAGTTCGACCACCAGCAGGTACGGGCCGTAGAGGCTGATGCCGACCGCCTTGGCGTCTACCGTGGtct           >  1:10514773/1‑148 (MQ=255)
                                                                                                                                       gcccgccGAACCTTTATTGTTTGGCGTCTTGACGACCCAGGTGATAGGCAGCGACCAGGGCCGCGAGCAACAGCATGGAGGCCAGTTCGACCACCAGCAGGTACGGGCCGTAGAGGCTGATGCCGACCGCCTTGGCGTCTACCGTGGTAt           <  1:1554696/150‑1 (MQ=255)
                                                                                                                                         ccgccgAACCTTTATTGTTTGGCGTCTTGACGACCCAGGTGATAGGCAGCGACCAGGGCCGCGAGCAACAGCATGGAGGCCAGTTCGACCACCAGCAGGTACGGGCCGTAGAGGCTGATGCCGACCGCCTTGGCGTCTACCGTGGTATgg         >  2:2452623/1‑150 (MQ=255)
                                                                                                                                           gccgAACCTTTATTGTTTGGCGTCTTGACGACCCAGGTGATAGGCAGCGACCAGGGCCGCGAGCAACAGCATGGAGGCCAGTTCGACCACCAGCAGGTACGGGCCGTAGAGGCTGATGCCGACCGCCTTGGCGTCTACCGTGGTATGGcc       <  2:2877530/150‑1 (MQ=255)
                                                                                                                                            ccgAACCTTTATTGTTTGGCGTCTTGACGACCCAGGTGATAGGCAGCGACCAGGGCCGCGAGCAACAGCATGGAGGCCAGTTCGACCACCAGCAGGTCCGGGCCGTAGAGGCTGATGCCGACCGCCTTGGCGTCTACCGTGGTATGGCCg      >  2:3157772/1‑150 (MQ=255)
                                                                                                                                             cgAACCTTTATTGTTTGGCGTCTTGACGACCCAGGTGATAGGCAGCGACCAGGGCCGCGAGCAACAGCATGGAGGCCAGTTCGACCACCAGCAGGTACGGGCCGTAGAGGCTGATGCCGACCGCCTTGGCGTCTACCGTGGTATGGCCGa     >  2:8129524/1‑150 (MQ=255)
                                                                                                                                             cgAACCTTTATTGTTTGGCGTCTTGACGACCCAGGTGATAGGCAGCGACCAGGGCCGCGAGCAACAGCATGGAGGCCAGTTCGACCACCAGCAGGTACGGGCCGTAGAGGCTGATGCCGACCGCCTTGGCGTCTACCGTGGTATGGCCGa     >  2:1297534/1‑150 (MQ=255)
                                                                                                                                              gAACCTTTATTGTTTGGCGTCTTGACGACCCAGGTGATAGGCAGCGACCAGGGCCGCGAGCAACAGCATGGAGGCCAGTTCGACCACCAGCCGGTACGGGCCGTAGAGGCTGATGCCGACCGCCTTGGCGTCTAGCGTGGTATGGCCGAt    >  1:5607289/1‑150 (MQ=255)
                                                                                                                                              gAACCTTTATTGTTTGGCGTCTTGACGACCCAGGTGATAGGCAGCGACCAGGGCCGCGAGCAACAGCATGGAGGCCAGTTCGACCACCAGCAGGTACGGGCCGTAGAGGCTGATGCCGACCGCCTTGGCGTCTACCGTGGTATGGCCGAt    <  2:6753014/150‑1 (MQ=255)
                                                                                                                                               aaCCTTTATTGTTTGGCGTCTTGACGACCCAGGTGATAGGCAGCGACCAGGGCCGCGAGCAACAGCATGGAGGCCAGTTCGACCACCAGCAGGTACGGGCCGTAGAGGCTGATGCCGACCGCCTTGGCGTCTACCGTGGTATGGCCGATg   >  2:4851874/1‑150 (MQ=255)
                                                                                                                                                aCCTTTATTGTTTGGCGTCTTGACGACCCAGGTGATAGGCAGCGACCAGGGCCGCGAGCAACAGCATGGAGGCCAGTTCGACCACCAGCAGGTACGGGCCGTAGAGGCTGATGCCGACCGCCTTGGCGTCTACCGTGGTATGGCCGATGc  >  1:6007015/1‑150 (MQ=255)
                                                                                                                                                aCCTTTATTGATTGGCGTCTTGACGACCCAGGTGATAGGCAGCGACCAGGGCCGCGAGCAACAGCATGGAGGCCAGTTCGACCACCAGCAGGTACGGGCCGTAGAGGCTGATGCCGACCGCCTTGGCGTCTACCGTGGTATGGCCGATGc  <  1:749124/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TCAGTACGAACAGGATGTTGCGTCGCACCATCAAGCCGACCAGTCCGAGGGCGAACAGGACGCTGGCGAGGGCCAGGCCGTGTTCCAGTGGTATTGCGTTCATAGCGTTACGTTCCCTTGGGGCGGGCGCAGGGCGCCCGCCGAACCCTTATTGTTTGGCGTCTTGACGACCCAGGTGATAGGCAGCGACCAGGGCCGCGAGCAACAGCATGGAGGCCAGTTCGACCACCAGCAGGTACGGGCCGTAGAGGCTGATGCCGACCGCCTTGGCGTCTACCGTGGTATGGCCGATGC  >  NC_008463/2589255‑2589548

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑