Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 2,762,027 A→G N126D (AAC→GAC)  PA14_RS12995 → lysophospholipid acyltransferase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084632,762,0270AG100.0% 1616.5 / NA 409N126D (AAC→GAC) PA14_RS12995lysophospholipid acyltransferase family protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (231/178);  total (231/178)

Only 100 of 423 total aligned reads displayed.

AGCTACGTGATCAACAAGGTGTTCAACGGCGTGCTGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCAAGGGCACCCGCAACCTGAACAACAAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCGGGTCATGCCCAAGGGCCGCATGCTGCCGTTGCCGCTGCTCTGC  >  NC_008463/2761880‑2762170
                                                                                                                                                   |                                                                                                                                               
aGCTACGTGATCAACAAGGTGTTCAACGGCGTGCTGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGgacgac                                                                                                                                               >  2:9577793/1‑150 (MQ=255)
aGCTACGTGATCAACAAGGTGTTCAACGGCGTGCTGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGgacgac                                                                                                                                               >  1:4267032/1‑150 (MQ=255)
aGCTACGTGATCAACAAGGTGTTCAACGGCGTGCTGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGgacgac                                                                                                                                               <  1:3435228/150‑1 (MQ=255)
  cTACGTGATCAACAAGGTGTTCAACGGCGTGCTGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGgacgacga                                                                                                                                             <  1:6279686/150‑3 (MQ=255)
  cTACGTGATCAACAAGGTGTTCAACGGCGTGCTGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGgacgacga                                                                                                                                             >  2:7025419/1‑148 (MQ=255)
  cTACGTGATCAACAAGGTGTTCAACGGCGTGCTGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGgacgacga                                                                                                                                             <  2:7583975/150‑3 (MQ=255)
    aCGTGATCAACAAGGTGTTCAACGGCGTGCTGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAgg                                                                                                                                           >  2:7450320/1‑150 (MQ=255)
    aCGTGATCAACAAGGTGTTCAACGGCGTGCTGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTACCCCGAGGGCACCCGCAACCTGGACGACGAgg                                                                                                                                           >  2:5285158/1‑150 (MQ=255)
     cGTGCTCAACAAGGTGTTCAACGGCGTGCTGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGt                                                                                                                                          <  1:7286418/150‑1 (MQ=255)
      gTGATCAACAAGGTGTTCAACGGCGTGCTGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTc                                                                                                                                         <  1:5695646/150‑1 (MQ=255)
       tGATCAACAAGGTGTTCAACGGCGTGCTGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCa                                                                                                                                        >  1:2890199/1‑150 (MQ=255)
            aacaaGGTGTTCAACGGCGTGCTGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTg                                                                                                                                   >  1:1049614/1‑150 (MQ=255)
              caaGGTGTTCAACGGCGTGCTGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGtt                                                                                                                                 >  1:9647116/1‑150 (MQ=255)
              caaGGTGTTCAACGGCGTGCTGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGtt                                                                                                                                 <  2:8045917/150‑1 (MQ=255)
               aaGGTGTTCAACGGCGTGCTGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTg                                                                                                                                >  1:1158808/1‑150 (MQ=255)
               aaGGTGTTCAACGGCGTGCTGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTg                                                                                                                                >  2:9499199/1‑150 (MQ=255)
                aGGTGTTCAACGGCGTGCTGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGc                                                                                                                               >  1:1729360/1‑150 (MQ=255)
                   tgtTCAACGGCGTGCTGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCAt                                                                                                                            <  1:4194055/150‑1 (MQ=255)
                       cAACGGCGTGCTGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCaa                                                                                                                        >  2:8903724/1‑150 (MQ=255)
                        aaCGGCGTGCTGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAag                                                                                                                       >  1:6856350/1‑150 (MQ=255)
                        aaCGGCGTGCTGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAag                                                                                                                       <  2:2553846/150‑1 (MQ=255)
                         aCGGCGTGCTGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAaga                                                                                                                      >  1:7216174/1‑150 (MQ=255)
                          cGGCGTGCTGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAagag                                                                                                                     <  2:3996022/150‑1 (MQ=255)
                          cGGCGTGCTGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAagag                                                                                                                     <  2:3120634/150‑1 (MQ=255)
                            gCGTGCTGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCg                                                                                                                   >  2:184614/1‑150 (MQ=255)
                             cGTGCTGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCgg                                                                                                                  <  2:9361385/150‑1 (MQ=255)
                              gTGCTGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGt                                                                                                                 >  1:7996436/1‑150 (MQ=255)
                              gTGCTGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGt                                                                                                                 >  1:10196295/1‑150 (MQ=255)
                                 cTGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATc                                                                                                              <  1:2338548/150‑1 (MQ=255)
                                  tGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCt                                                                                                             <  2:1000337/150‑1 (MQ=255)
                                   ggTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTa                                                                                                            >  1:10650434/1‑150 (MQ=255)
                                   ggTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTa                                                                                                            >  2:2178341/1‑150 (MQ=255)
                                        aCCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTaccacc                                                                                                       <  2:328098/150‑1 (MQ=255)
                                        aCCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTaccacc                                                                                                       >  1:2616447/1‑150 (MQ=255)
                                         ccGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCt                                                                                                      >  2:10555663/1‑150 (MQ=255)
                                         ccGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCt                                                                                                      <  1:5245271/150‑1 (MQ=255)
                                          cGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTg                                                                                                     <  2:5136241/150‑1 (MQ=255)
                                            ccAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTc                                                                                                   >  1:3740399/1‑150 (MQ=255)
                                                cgcagcgATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTg                                                                                               <  1:8620681/150‑1 (MQ=255)
                                                  cagcgATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGc                                                                                             >  2:3314079/1‑150 (MQ=255)
                                                  cagcgATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGc                                                                                             <  1:6127991/150‑1 (MQ=255)
                                                    gcgATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGccc                                                                                           >  1:6609537/1‑150 (MQ=255)
                                                    gcgATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGccc                                                                                           <  1:10413634/150‑1 (MQ=255)
                                                      gATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCAc                                                                                         <  1:8839610/150‑1 (MQ=255)
                                                      gATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCAGGACGGCTGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGTTCAAGCTTTTGCCATTCAAGAGCGGTATCTAACACCTGTCCCTGGCCCAc                                                                                         <  2:2650671/150‑1 (MQ=255)
                                                       atgccaatgccaATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCAcc                                                                                        <  1:472535/150‑1 (MQ=255)
                                                        tgccaatgccaaTCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCAccc                                                                                       <  1:9882550/150‑1 (MQ=255)
                                                        tgccaatgccaaTCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCAccc                                                                                       >  1:5046709/1‑150 (MQ=255)
                                                         gccaatgccaatCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCg                                                                                      >  2:9108184/1‑150 (MQ=255)
                                                             atgccaatCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAgg                                                                                  >  2:6827054/1‑150 (MQ=255)
                                                             atgccaatCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAgg                                                                                  >  1:5484555/1‑150 (MQ=255)
                                                             atgccaatCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAgg                                                                                  >  2:5597565/1‑150 (MQ=255)
                                                              tgccaatCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGt                                                                                 <  1:7747028/150‑1 (MQ=255)
                                                                  aatCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAg                                                                             <  1:4603742/150‑1 (MQ=255)
                                                                  aatCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAg                                                                             >  2:6299063/1‑150 (MQ=255)
                                                                   atCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCTACCCGGAGGTCGAGc                                                                            >  1:8470061/1‑150 (MQ=255)
                                                                   atCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGc                                                                            >  2:1193497/1‑150 (MQ=255)
                                                                      cGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTgg                                                                         >  1:7786625/1‑150 (MQ=255)
                                                                      cGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTgg                                                                         <  2:60/150‑1 (MQ=255)
                                                                       gCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGt                                                                        >  1:4478598/1‑150 (MQ=255)
                                                                       gCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGt                                                                        >  1:6995696/1‑150 (MQ=255)
                                                                           gAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGGTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCg                                                                    >  2:4630355/1‑150 (MQ=255)
                                                                            aaCCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCgg                                                                   >  2:1410569/1‑150 (MQ=255)
                                                                               cccTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTct                                                                >  1:1389284/1‑150 (MQ=255)
                                                                                  tCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTctggc                                                             >  1:10314061/1‑150 (MQ=255)
                                                                                   cGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTctggct                                                            >  2:1926927/1‑150 (MQ=255)
                                                                                      ccGCGCCCTGGCCGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCtggctggc                                                         <  2:4816326/150‑1 (MQ=255)
                                                                                           cccTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAAcc                                                    >  1:6016902/1‑150 (MQ=255)
                                                                                           cccTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAAcc                                                    >  2:717487/1‑150 (MQ=255)
                                                                                            ccTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCt                                                   >  2:3626691/1‑150 (MQ=255)
                                                                                             cTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTc                                                  <  1:6417839/150‑1 (MQ=255)
                                                                                             cTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTc                                                  <  2:2903587/150‑1 (MQ=255)
                                                                                              tGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCa                                                 >  2:3461661/1‑150 (MQ=255)
                                                                                               ggACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCaa                                                >  1:3037830/1‑150 (MQ=255)
                                                                                                gACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAAc                                               <  1:2006354/150‑1 (MQ=255)
                                                                                                 aCGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAAcc                                              >  1:3254172/1‑150 (MQ=255)
                                                                                                   ggcggcGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCCACCgg                                            >  2:4327717/1‑150 (MQ=255)
                                                                                                   ggcggcGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCgg                                            <  1:2360242/150‑1 (MQ=255)
                                                                                                   ggcggcGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCgg                                            <  2:1698332/150‑1 (MQ=255)
                                                                                                       gcgATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCGGGTCa                                        >  1:2232316/1‑150 (MQ=255)
                                                                                                         gATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCGGGTCATg                                      <  2:7295221/150‑1 (MQ=255)
                                                                                                               cTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCGGGTCATGCCCAAg                                <  2:8375856/150‑1 (MQ=255)
                                                                                                               cTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCGGGTCATGCCCAAg                                >  2:872134/1‑150 (MQ=255)
                                                                                                                tGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCGGGTCATGCCCAAgg                               <  1:10009244/150‑1 (MQ=255)
                                                                                                                tGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCGGGTCATGCCCAAgg                               >  1:10545031/1‑150 (MQ=255)
                                                                                                                  atcatcTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCGGGTCATGCCCAAGGGc                             <  2:4198675/150‑1 (MQ=255)
                                                                                                                   tcatcTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCGGGTCATGCCCAAGGGcc                            >  2:6565936/1‑150 (MQ=255)
                                                                                                                   tcatcTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCGGGTCATGCCCAAGGGcc                            <  2:2407474/150‑1 (MQ=255)
                                                                                                                          ccccGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCGGGTCATGCCCAAGGGCCGCAtgct                     >  1:7554717/1‑150 (MQ=255)
                                                                                                                            ccgaGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCGGGTCATGCCCAAGGGCCGCAtgctgc                   >  2:10427576/4‑150 (MQ=255)
                                                                                                                               tgggCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACGTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCGGGTCATGCCCAAGGGCCGCATGCTGCCGt                >  1:3467306/2‑150 (MQ=255)
                                                                                                                                gggCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCGGGTCATGCCCAAGGGCCGCATGCTGCCGtt               >  1:9088116/1‑150 (MQ=255)
                                                                                                                                 ggCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCGGGTCATGCCCAAGGGCCGCATGCTGCCGTTg              >  1:8327609/1‑150 (MQ=255)
                                                                                                                                  gCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCGGGTCATGCCCAAGGGCCGCATGCTGCCGTTGc             <  2:4960272/150‑1 (MQ=255)
                                                                                                                                  gCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCGGGTCATGCCCAAGGGCCGCATGCTGCCGTTGc             <  2:1087039/150‑1 (MQ=255)
                                                                                                                                     cccGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCACCCGGGTCATGCCCAAGGGCCGCATGCGGCCGTTGCCgc          <  2:6906076/150‑1 (MQ=255)
                                                                                                                                     cccGCAACCTGGACGACGAGGTCAAGCTGTTACCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCGGGTCATGCCCAAGGGCCGCATGCTGCCGTTGCCgc          >  2:7697268/1‑150 (MQ=255)
                                                                                                                                       cGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCGGGTCATGCCCAAGGGCCGCATGCTGCCGTTGCCgctg        >  1:2562888/1‑150 (MQ=255)
                                                                                                                                            ccTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCGGGTCATGCCCAAGGGCCGCATGCTGCCGTTGCCGCTGCTCTg   >  2:10117021/1‑150 (MQ=255)
                                                                                                                                            ccTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCGGGTCATGCCCAAGGGCCGCATGCTGCCGTTGCCGCTGCTCTg   >  2:9764942/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                               
AGCTACGTGATCAACAAGGTGTTCAACGGCGTGCTGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCAAGGGCACCCGCAACCTGAACAACAAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCGGGTCATGCCCAAGGGCCGCATGCTGCCGTTGCCGCTGCTCTGC  >  NC_008463/2761880‑2762170

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑