Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 2,762,030 A→G K127E (AAG→GAG)  PA14_RS12995 → lysophospholipid acyltransferase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084632,762,0300AG100.0% 1642.2 / NA 409K127E (AAG→GAG) PA14_RS12995lysophospholipid acyltransferase family protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (231/178);  total (231/178)

Only 100 of 409 total aligned reads displayed.

TACGTGATCAACAAGGTGTTCAACGGCGTGCTGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCAAGGGCACCCGCAACCTGAACAACAAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCGGGTCATGCCCAAGGGCCGCATGCTGCCGTTGCCGCTGCTCTGC  >  NC_008463/2761883‑2762170
                                                                                                                                                   |                                                                                                                                            
tACGTGATCAACAAGGTGTTCAACGGCGTGCTGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAg                                                                                                                                            >  1:6412010/1‑150 (MQ=255)
tACGTGATCAACAAGGAGTTCAACGGCGTGCTGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAg                                                                                                                                            <  2:2916347/150‑1 (MQ=255)
 aCGTGATCAACAAGGTGTTCAACGGCGTGCTGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTACCCCGAGGGCACCCGCAACCTGGACGACGAgg                                                                                                                                           >  2:5285158/1‑150 (MQ=255)
  cGTGCTCAACAAGGTGTTCAACGGCGTGCTGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGt                                                                                                                                          <  1:7286418/150‑1 (MQ=255)
  cGTGATCAACAAGGTGTTCAACGGCGTGCTGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGt                                                                                                                                          >  2:4521572/1‑150 (MQ=255)
  cGTGATCAACAAGGTGTTCAACGGCGTGCTGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGt                                                                                                                                          >  2:3544714/1‑150 (MQ=255)
   gTGATCAACAAGGTGTTCAACGGCGTGCTGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTc                                                                                                                                         <  1:6037418/150‑1 (MQ=255)
   gTGATCAACAAGGTGTTCAACGGCGTGCTGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTc                                                                                                                                         >  2:9492081/1‑150 (MQ=255)
    tGATCAACAAGGTGTTCAACGGCGTGCTGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCa                                                                                                                                        >  1:2890199/1‑150 (MQ=255)
     gATCAACAAGGTGTTCAACGGCGTGCTGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCaa                                                                                                                                       >  2:7761683/1‑150 (MQ=255)
       tCAACAAGGTGTTCAACGGCGTGCTGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGc                                                                                                                                     <  1:4710886/150‑1 (MQ=255)
        caacaaGGTGTTCAACGGCGTGCTGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCt                                                                                                                                    >  2:5582901/1‑150 (MQ=255)
           caaGGTGTTCAACGGCGTGCTGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGtt                                                                                                                                 >  1:8061752/1‑150 (MQ=255)
             aGGTGTTCAACGGCGTGCTGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGc                                                                                                                               >  1:1729360/1‑150 (MQ=255)
              ggtggtCAACGGGGTGCTGGTCGACCGCCAGAGCAGCGATGCCAATGCCATTCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCGTCCCCGAGGGCACCCGCAACCTGGACGACGTGGTCAAGCTGTTGcc                                                                                                                              <  2:3329601/150‑1 (MQ=255)
                tgtTCAACGGCGTGCTGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCAt                                                                                                                            <  1:4194055/150‑1 (MQ=255)
                 gtTCAACGGCGTGCTGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCAtt                                                                                                                           >  1:751762/1‑150 (MQ=255)
                    cAACGGCGTGCTGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCaa                                                                                                                        >  2:8903724/1‑150 (MQ=255)
                     aaCGGCGTGCTGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAag                                                                                                                       >  1:5263654/1‑150 (MQ=255)
                     aaCGGCGTGCTGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAag                                                                                                                       <  2:2553908/150‑1 (MQ=255)
                       cGGCGTGCTGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAagag                                                                                                                     <  2:3120634/150‑1 (MQ=255)
                       cGGCGTGCTGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAagag                                                                                                                     <  2:3996022/150‑1 (MQ=255)
                         gCGTGCTGGTCGACCGCCAGCGCAGCGATGCCAATGCCACTCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCg                                                                                                                   <  1:9066387/150‑1 (MQ=255)
                          cGTGCTGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCgg                                                                                                                  <  2:9361284/150‑1 (MQ=255)
                           gTGCTGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGt                                                                                                                 >  1:10196295/1‑150 (MQ=255)
                                 gTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTac                                                                                                           <  2:2197265/150‑1 (MQ=255)
                                  tCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTacc                                                                                                          <  1:4829253/150‑1 (MQ=255)
                                     aCCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTaccacc                                                                                                       >  1:769719/1‑150 (MQ=255)
                                     aCCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTaccacc                                                                                                       >  1:2616447/1‑150 (MQ=255)
                                       cGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTg                                                                                                     >  1:1044645/1‑150 (MQ=255)
                                          cagcgcagcgATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTAACTACCACCTGTcc                                                                                                  >  1:2347756/1‑150 (MQ=255)
                                           agcgcagcgATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTccc                                                                                                 <  1:9881995/150‑1 (MQ=255)
                                            gcgcagcgATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCt                                                                                                >  1:7956285/1‑150 (MQ=255)
                                            gcgcagcgATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCt                                                                                                <  1:6967698/150‑1 (MQ=255)
                                             cgcagcgATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTg                                                                                               <  1:3788582/150‑1 (MQ=255)
                                             cgcagcgATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTg                                                                                               <  1:8620681/150‑1 (MQ=255)
                                                agcgATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGcc                                                                                            <  2:4929214/150‑1 (MQ=255)
                                                 gcgATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGccc                                                                                           >  2:6118813/1‑150 (MQ=255)
                                                   gATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCAc                                                                                         <  1:8839610/150‑1 (MQ=255)
                                                   gATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCAc                                                                                         <  1:1052697/150‑1 (MQ=255)
                                                   gATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCAc                                                                                         <  1:9645625/150‑1 (MQ=255)
                                                      gccaatgccaatCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCg                                                                                      >  2:9108184/1‑150 (MQ=255)
                                                       ccaatgccaatCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCgg                                                                                     <  1:8376218/150‑1 (MQ=255)
                                                         aatgccaatCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAg                                                                                   <  2:10499891/150‑1 (MQ=255)
                                                          atgccaatCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAgg                                                                                  >  2:9571653/1‑150 (MQ=255)
                                                           tgccaatCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGt                                                                                 <  1:2470430/150‑1 (MQ=255)
                                                             ccaatCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCg                                                                               <  2:8092413/150‑1 (MQ=255)
                                                              caatCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGa                                                                              >  1:10347704/1‑150 (MQ=255)
                                                              caatCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGa                                                                              >  2:74058/1‑150 (MQ=255)
                                                               aatCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAg                                                                             >  1:4526905/1‑150 (MQ=255)
                                                                atCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGc                                                                            >  1:5829086/1‑150 (MQ=255)
                                                                   cGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCACGCTGTTGCCATTCAAGTGCGGTATCTACCACCTGTCCCTGGCCCCCCCGGAGGTCGAGCTgg                                                                         >  1:1070796/1‑150 (MQ=255)
                                                                   cGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCNTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTgg                                                                         >  1:5631342/1‑150 (MQ=255)
                                                                       cGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGcc                                                                     >  2:3283658/1‑150 (MQ=255)
                                                                         aaCCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCgg                                                                   >  1:3360562/1‑150 (MQ=255)
                                                                           ccccTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTc                                                                 <  2:172888/150‑1 (MQ=255)
                                                                                cGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTctggct                                                            >  2:1927322/1‑150 (MQ=255)
                                                                                  aCCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTctggctgg                                                          >  2:4796661/1‑150 (MQ=255)
                                                                                  aCCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTctggctgg                                                          >  1:3491644/1‑150 (MQ=255)
                                                                                  aCCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTctggctgg                                                          >  2:765382/1‑150 (MQ=255)
                                                                                     gcgcCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCa                                                       <  2:7090867/150‑1 (MQ=255)
                                                                                         ccTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCt                                                   >  1:6554643/1‑150 (MQ=255)
                                                                                         ccTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCt                                                   >  2:3626691/1‑150 (MQ=255)
                                                                                          cTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTc                                                  >  1:1057890/1‑150 (MQ=255)
                                                                                          cTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTc                                                  <  1:6853969/150‑1 (MQ=255)
                                                                                          cTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTc                                                  >  2:2686939/1‑150 (MQ=255)
                                                                                            ggACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCaa                                                >  1:3037830/1‑150 (MQ=255)
                                                                                             gACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAAc                                               <  1:2006354/150‑1 (MQ=255)
                                                                                              aCGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAAcc                                              <  1:7783617/150‑1 (MQ=255)
                                                                                              aCGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAAcc                                              <  1:5228446/150‑1 (MQ=255)
                                                                                                ggcggcGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCCACCgg                                            >  2:4327717/1‑150 (MQ=255)
                                                                                                ggcggcGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCgg                                            >  2:5107503/1‑150 (MQ=255)
                                                                                                 gcggcgATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCggg                                           >  1:3107802/1‑150 (MQ=255)
                                                                                                    gcgATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCGGGTCa                                        >  1:2232316/1‑150 (MQ=255)
                                                                                                    gcgATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCGGGTCa                                        >  2:6481819/1‑150 (MQ=255)
                                                                                                      gATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGGTGCCATTCAAGAGCGGTATCTACCACCGGTCCCTGGCCCACACGTAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCGGGTCATg                                      <  2:1922755/150‑1 (MQ=255)
                                                                                                        ttCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCGGGTCATGcc                                    >  1:6145724/1‑150 (MQ=255)
                                                                                                            cTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCGGGTCATGCCCAAg                                <  2:8375856/150‑1 (MQ=255)
                                                                                                            cTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCGGGTCATGCCCAAg                                >  2:872134/1‑150 (MQ=255)
                                                                                                             tGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCGGGTCATGCCCAAgg                               <  2:10327321/150‑1 (MQ=255)
                                                                                                             tGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCGGGTCATGCCCAAgg                               <  1:10009244/150‑1 (MQ=255)
                                                                                                               atcatcTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCGGGTCATGCCCAAGGGc                             <  2:4198675/150‑1 (MQ=255)
                                                                                                               atcatcTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCGGGTCATGCCCAAGGGc                             >  2:677991/1‑150 (MQ=255)
                                                                                                                tcatcTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCGGGTCATGCCCAAGGGcc                            >  1:9975106/1‑150 (MQ=255)
                                                                                                                 catcTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCGGGTCATGCCCAAGGGCCg                           >  2:1218443/1‑150 (MQ=255)
                                                                                                                   tcTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCGGGTCATGCCCAAGGGCCGCa                         >  2:7278563/1‑150 (MQ=255)
                                                                                                                   tcTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCGGGTCATGCCCAAGGGCCGCa                         <  2:1473725/150‑1 (MQ=255)
                                                                                                                   tcTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCGGGTCATGCCCAAGGGCCGCa                         >  1:1441023/1‑150 (MQ=255)
                                                                                                                   tcTTCCCCGAGGGCACCCGCAACCTGGACGAAGAGGTCAAGCTGTAGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCTAGCTGGTGCCGGTCAGGCTGGCCAACATCAACCGGGTCATGCCCAAGTGCCGCa                         >  1:7213017/1‑150 (MQ=255)
                                                                                                                       ccccGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCGGGTCATGCCCAAGGGCCGCAtgct                     <  2:5907122/150‑1 (MQ=255)
                                                                                                                          cgaGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGTGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCGGGTCATGCCCAAGGGCCGCATGCTGcc                  <  2:6875950/148‑1 (MQ=255)
                                                                                                                          cgaGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCGGGTCATGCCCAAGGGCCGCATGCTGcc                  <  2:6975724/148‑1 (MQ=255)
                                                                                                                            aGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCGGGTCATGCCCAAGGGCCGCATGCTGCCGt                >  2:10035409/1‑150 (MQ=255)
                                                                                                                            aGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCGGGTCATGCCCAAGGGCCGCATGCGGCCGt                <  2:1157273/150‑1 (MQ=255)
                                                                                                                                cACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCGGGTCATGCCCAAGGGCCGCATGCTGCCGTTGcc            >  2:10656352/1‑150 (MQ=255)
                                                                                                                                cACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCGGGTCATGCCCAAGGGCCGCATGCTGCCGTTGcc            <  1:4429958/150‑1 (MQ=255)
                                                                                                                                 aCCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCGGGTCATGCCCAAGGGCCGCATGCTGCCGTTGCCg           >  1:119661/1‑150 (MQ=255)
                                                                                                                                  cccGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCGGGTCATGCCCAAGGGCCGCATGCTGCCGTTGCCgc          >  2:2424583/1‑150 (MQ=255)
                                                                                                                                    cGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCGGGTCATGCCCAAGGGCCGCATGCTGCCGTTGCCgctg        >  1:2562888/1‑150 (MQ=255)
                                                                                                                                         ccTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCGGGTCATGCCCAAGGGCCGCATGCTGCCGTTGCCGCTGCTCTg   >  2:9764942/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                            
TACGTGATCAACAAGGTGTTCAACGGCGTGCTGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCAAGGGCACCCGCAACCTGAACAACAAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCGGGTCATGCCCAAGGGCCGCATGCTGCCGTTGCCGCTGCTCTGC  >  NC_008463/2761883‑2762170

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑