Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 4,957,549 2 bp→AT intergenic (‑371/‑300) PA14_RS22700 ← / → PA14_RS22705 16S ribosomal RNA/exonuclease SbcCD subunit D C‑terminal domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084634,957,5490GA100.0% 541.8 / NA 267intergenic (‑371/‑301)PA14_RS22700/PA14_RS2270516S ribosomal RNA/exonuclease SbcCD subunit D C‑terminal domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (123/144);  total (123/144)
*NC_0084634,957,5500CT100.0% 542.4 / NA 267intergenic (‑372/‑300)PA14_RS22700/PA14_RS2270516S ribosomal RNA/exonuclease SbcCD subunit D C‑terminal domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (123/144);  total (123/144)

Only 100 of 267 total aligned reads displayed.

TGCGCCGACCTTCAAACCATCTTCCGATCAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGATGAAGCTTCGTTTCGAGCCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTTCGTCGTCGCCGGGAGTGGTGCGCATTATAGGGAGATAGAAACT  >  NC_008463/4957455‑4957693
                                                                                              ||                                                                                                                                               
 gcgcCGACCTTCAAACCATCTTCCGATCAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTc                                                                                          >  1:5077984/1‑150 (MQ=9)
  cgcCGACCTTCAAACCATCTTCCGATCAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTcc                                                                                         <  1:148704/150‑1 (MQ=12)
  cgcCGACCTTCAAACCATCTTCCGATCAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTcc                                                                                         <  1:6449870/150‑1 (MQ=12)
   gcCGACCTTCAAACCATCTTCCGATCAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCt                                                                                        >  2:1272579/1‑150 (MQ=12)
    ccGACCTTCAAACCATCTTCCGATCAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTg                                                                                       >  2:890440/1‑150 (MQ=12)
    ccGACCTTCAAACCATCTTCCGATCAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTg                                                                                       >  1:664912/1‑150 (MQ=12)
    ccGACCTTCAAACCATCTTCCGATCAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTg                                                                                       >  1:3036823/1‑150 (MQ=12)
    ccGACCGTCAAACCAGCTTCCGATCAGCGGGAGGCGCAGTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCCCTCCTg                                                                                       >  2:6407128/1‑150 (MQ=11)
      gACCTTCAAACCATCTTCCGATCAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTggg                                                                                     <  1:1767868/150‑1 (MQ=12)
       aCCTTCAAACCATCTTCCGATCAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGga                                                                                    >  2:7658751/1‑150 (MQ=12)
        ccTTCAAACCATCTTCCGATCAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGgag                                                                                   <  2:790149/150‑1 (MQ=12)
        ccTTCAAACCATCTTCCGATCAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGgag                                                                                   >  1:2770509/1‑150 (MQ=12)
         cTTCAAACCATCTTCCGATCAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGgaga                                                                                  >  2:6915918/1‑150 (MQ=12)
         cTTCAAACCATCTTCCGATCAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGgaga                                                                                  <  1:3911656/150‑1 (MQ=12)
         cTTCAAACCATCTTCCGATCAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGgaga                                                                                  <  1:7237802/150‑1 (MQ=12)
          ttCAAACCATCTTCCGATCAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGgagag                                                                                 <  1:10309288/150‑1 (MQ=12)
          ttCAAACCATCTTCCGATCAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGgagag                                                                                 >  1:4234001/1‑150 (MQ=12)
           tCAAACCATCTTCCGATCAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGgagaga                                                                                <  2:3289433/150‑1 (MQ=12)
              aaCCATCTTCCGATCAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATc                                                                             >  2:4896752/1‑150 (MQ=12)
                   tCTTCCGATCAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTaa                                                                        >  1:7863033/1‑150 (MQ=12)
                    cTTCCGATCAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTaaa                                                                       <  2:4401540/150‑1 (MQ=12)
                    cTTCCGATCAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTaaa                                                                       >  1:8598482/1‑150 (MQ=12)
                     ttCCGATCAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAAt                                                                      <  2:6185189/150‑1 (MQ=12)
                      tCCGATCAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATc                                                                     <  1:2976853/150‑1 (MQ=12)
                      tCCGATCAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATc                                                                     <  1:5776829/150‑1 (MQ=12)
                       ccGATCAGCGGGAGGCGCATTCTACAGCGTTCAAAGTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATcc                                                                    <  2:1690668/150‑1 (MQ=255)
                         gATCAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCtt                                                                  >  1:8234776/1‑150 (MQ=12)
                          aTCAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTg                                                                 <  1:7653291/150‑1 (MQ=12)
                           tCAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGa                                                                >  2:11570/1‑150 (MQ=12)
                           tCAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGa                                                                <  1:1180418/150‑1 (MQ=12)
                           tCAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGa                                                                >  1:4083158/1‑150 (MQ=12)
                            cAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGAt                                                               <  2:3430300/150‑1 (MQ=12)
                            cAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGAt                                                               <  1:8316209/150‑1 (MQ=12)
                             aGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGAtt                                                              <  1:9011435/150‑1 (MQ=12)
                             aGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGAtt                                                              <  2:5541321/150‑1 (MQ=12)
                              gCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGAttt                                                             >  1:3394841/1‑150 (MQ=12)
                              gCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGAttt                                                             >  2:5412241/1‑150 (MQ=12)
                               cGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGAtttt                                                            <  1:5871255/150‑1 (MQ=12)
                               cGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGAtttt                                                            >  2:2178774/1‑150 (MQ=12)
                                 ggAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCa                                                          >  2:7528607/1‑150 (MQ=12)
                                 ggAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCa                                                          >  1:7511295/1‑150 (MQ=12)
                                 ggAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCa                                                          >  1:450452/1‑150 (MQ=12)
                                    ggCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGc                                                       <  2:1772179/150‑1 (MQ=12)
                                       gcATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCAtt                                                    >  2:2569178/1‑150 (MQ=12)
                                         aTTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTa                                                  >  2:5330066/1‑150 (MQ=12)
                                         aTTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTa                                                  <  1:6948905/150‑1 (MQ=12)
                                         aTTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTa                                                  <  1:4281685/150‑1 (MQ=12)
                                           tcgaCAGCGTTCAAATTCGCTGTCAAGCACCACGGTGACGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCCGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTAct                                                <  2:6840438/147‑1 (MQ=11)
                                            cTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTActc                                               <  1:9438570/150‑1 (MQ=12)
                                            cTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTActc                                               >  2:370829/1‑150 (MQ=12)
                                            cTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTActc                                               <  1:467873/150‑1 (MQ=12)
                                             tACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTActct                                              <  2:2292807/150‑1 (MQ=12)
                                              aCAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCtt                                             >  1:10448087/1‑150 (MQ=12)
                                              aCAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCtt                                             <  1:2044436/150‑1 (MQ=12)
                                                aGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTtcg                                           <  1:10136686/150‑1 (MQ=12)
                                                aGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTtcg                                           >  1:2245634/1‑150 (MQ=12)
                                                aGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTtcg                                           <  2:9206675/150‑1 (MQ=12)
                                                aGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTtcg                                           >  2:8542052/1‑150 (MQ=12)
                                                 gCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACACCCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTtcgt                                          <  2:3828734/150‑1 (MQ=255)
                                                 gCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTtcgt                                          <  2:4746766/150‑1 (MQ=12)
                                                 gCGTTCAAAGTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTtcgt                                          <  1:6323285/150‑1 (MQ=255)
                                                  cGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTtcgtc                                         <  1:134722/150‑1 (MQ=12)
                                                   gTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTtcgtcg                                        >  2:5015113/1‑150 (MQ=12)
                                                    ttCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTtcgtcgt                                       <  1:4623986/150‑1 (MQ=12)
                                                    ttCAAAGTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTtcgtcgt                                       <  2:8499078/150‑1 (MQ=255)
                                                     tCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTtcgtcgtc                                      >  2:3799120/1‑150 (MQ=12)
                                                     tCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTtcgtcgtc                                      >  2:5271113/1‑150 (MQ=12)
                                                      cAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTtcgtcgtcg                                     <  1:7114945/150‑1 (MQ=12)
                                                         aTTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTTCGTCGTCGCCg                                  >  2:2691431/1‑150 (MQ=12)
                                                          ttCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTTCGTCGTCGCCgg                                 >  2:3194811/1‑150 (MQ=12)
                                                            cGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTTCGTCGTCGCCGGGa                               <  2:9668357/150‑1 (MQ=12)
                                                            cGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTTCGTCGTCGCCGGGa                               >  1:3677225/1‑150 (MQ=12)
                                                             gCTGTCAAGCCCCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGCGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTTCGTCGTCGCCGGGAg                              >  2:10390349/1‑150 (MQ=11)
                                                              cTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTTCGTCGTCGCCGGGAgt                             >  2:2982651/1‑150 (MQ=12)
                                                              cTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTTCGTCGTCGCCGGGAgt                             <  2:717810/150‑1 (MQ=12)
                                                               tGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTTCGTCGTCGCCGGGAgtg                            >  1:9561084/1‑150 (MQ=12)
                                                                 tCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTACGTCGTCGCCGGGAgtggt                          <  1:3284909/150‑1 (MQ=255)
                                                                    aGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTTCGTCGTCGCCGGGAGTGGTgcg                       >  2:8163441/1‑150 (MQ=12)
                                                                     gCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTTCGTCGTCGCCGGGAGTGGTgcgc                      <  2:1609962/150‑1 (MQ=12)
                                                                     gCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCAGTTACTCTTCGTCGTCGCCGGGAGTGGTgcgc                      >  2:10334830/1‑150 (MQ=255)
                                                                      cACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTTCGTCGTCGCCGGGAGTGGTGCGCa                     >  2:10709790/1‑150 (MQ=12)
                                                                       aCCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTTCGTCGTCGCCGGGAGTGGTGCGCAt                    <  1:927827/150‑1 (MQ=12)
                                                                        ccTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTTCGTCGTCGCCGGGAGTGGTGCGCAtt                   <  1:1809722/150‑1 (MQ=12)
                                                                          tCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTTCGTCGTCGCCGGGAGTGGTGCGCATtat                 <  1:868896/150‑1 (MQ=12)
                                                                          tCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTTCGTCGTCGCCGGGAGTGGTGCGCATtat                 >  1:10556616/1‑150 (MQ=12)
                                                                            ggtGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCGTCGTCGTCGCCGGGAGTGGTGCGCCTTATAg               >  1:1929679/3‑150 (MQ=255)
                                                                             gtGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTTCGTCGTCGCCGGGAGTGGTGCGCATTATAgg              >  1:114780/2‑150 (MQ=17)
                                                                               gAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTTCGTCGTCGCCGGGAGTGGTGCGCATTATAGGga            <  1:2560856/150‑1 (MQ=17)
                                                                               gAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGACGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATACTTGATTTTCAAGAATTAACTCTTCGACGTCGCCGGGAGTGGTGCGCATTATAGGga            >  1:9854941/1‑150 (MQ=11)
                                                                                aaGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTTCGTCGTCGCCGGGAGTGGTGCGCATTATAGGgag           <  2:7070805/150‑1 (MQ=17)
                                                                                aaGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTTCGTCGTCGCCGGGAGTGGTGCGCATTATAGGgag           <  1:9191135/150‑1 (MQ=17)
                                                                                 aGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTTCGTCGTCGCCGGGAGTGGTGCGCATTATAGGgaga          <  2:2328699/150‑1 (MQ=17)
                                                                                 aGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTTCGTCGTCGCCGGGAGTGGTGCGCATTATAGGgaga          <  2:4259523/150‑1 (MQ=17)
                                                                                   cTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTTCGTCGTCGCCGGGAGTGGTGCGCAGTATAGGGAGATa        >  1:2363784/1‑150 (MQ=12)
                                                                                    ttCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTTCGTCGTCGCCGGGAGTGGTGCGCATTATAGGGAGATAg       >  2:8724587/1‑150 (MQ=17)
                                                                                       gTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTTCGTCGTCGCCGGGAGTGGTGCGCATTATAGGGAGATAGaaa    <  2:4043820/150‑1 (MQ=17)
                                                                                       gTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTTCGTCGTCGCCGGGAGTGGTGCGCATTATAGGGAGATAGaaa    <  1:10633591/150‑1 (MQ=17)
                                                                                        tttCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTTCGTCGTCGCCGGGAGTGGTGCGCATTATAGGGAGATAGAAAc   <  1:8351685/150‑1 (MQ=17)
                                                                                        tttCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTTCGTCGTCGCCGGGAGTGGTGCGCATTATAGGGAGATAGAAAc   <  1:9594450/150‑1 (MQ=17)
                                                                                         ttCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTTCGTCGTCGCCGGGAGTGGTGCGCATTATAGGGAGATAGAAACt  <  1:5621236/150‑1 (MQ=17)
                                                                                              ||                                                                                                                                               
TGCGCCGACCTTCAAACCATCTTCCGATCAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGATGAAGCTTCGTTTCGAGCCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTTCGTCGTCGCCGGGAGTGGTGCGCATTATAGGGAGATAGAAACT  >  NC_008463/4957455‑4957693

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑