Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 791,862 3 bp→GCT coding (1842‑1844/5430 nt) pchF → pyochelin non‑ribosomal peptide synthetase PchF

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_008463791,8620CG99.6% 1897.0 / ‑4.5 489A614A (GCC→GCG) pchFpyochelin non‑ribosomal peptide synthetase PchF
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base G (296/190);  total (297/192)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*NC_008463791,8630TC99.4% 1903.2 / ‑1.4 489W615M (TGG→CTG) pchFpyochelin non‑ribosomal peptide synthetase PchF
Reads supporting (aligned to +/- strand):  ref base T (1/2);  new base C (295/189);  total (297/192)
Fisher's exact test for biased strand distribution p-value = 5.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*NC_008463791,8640GT99.2% 2061.6 / ‑4.3 489W615M (TGG→CTG) pchFpyochelin non‑ribosomal peptide synthetase PchF
Reads supporting (aligned to +/- strand):  ref base G (2/2);  new base T (295/189);  total (297/192)
Fisher's exact test for biased strand distribution p-value = 6.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 489 total aligned reads displayed.

CGCGGTGATGCTGCCGAAAAGCGCCGCGCAATTGGTCGCGGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCCCCGAAGTGGTCGCCTGGGTCTGCCTGGAAAGCGATGTCCCGAACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTGGCGGCGGACGACCTCGCCTACGTGATCTACACCTCCG  >  NC_008463/791721‑792004
                                                                                                                                             |||                                                                                                                                            
  cgGTGATGCTGCCGAAAAGCGCCGCGCAATTGGTCGCGGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGcc                                                                                                                                      >  2:5656628/1‑150 (MQ=255)
   ggTGATGCTGCCGAAAAGCGCCGCGCAATTGGTCGCGGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCt                                                                                                                                     <  1:4501751/150‑1 (MQ=255)
   ggTGATGCTGCCGAAAAGCGCCGCGCAATTGGTCGCGGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCt                                                                                                                                     <  2:5246862/150‑1 (MQ=255)
     tGATGCTGCCGAAAAGCGCCGCGCAATTGGTCGCGGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTgg                                                                                                                                   >  2:3616846/1‑150 (MQ=255)
         gctgcCGAAAAGCGCCGCGCAATTGGTCGCGGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAg                                                                                                                               <  2:8024878/150‑1 (MQ=255)
          ctgcCGAAAAGCGCCGCGCAATTGGTCGCGGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGc                                                                                                                              <  1:816367/150‑1 (MQ=255)
          ctgcCGAAAAGCGCCGCGCAATTGGTCGCGGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGc                                                                                                                              >  2:4408050/1‑150 (MQ=255)
          ctgcCGAAAAGCGCCGCGCAATTGGTCGCGGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGc                                                                                                                              >  2:4996184/1‑150 (MQ=255)
            gcCGAAAAGCGCCGCGCAATTGGTCGCGGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGa                                                                                                                            >  1:10296750/1‑150 (MQ=255)
                aaaaGCGCCGCGCAATTGGTCGCGGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTc                                                                                                                        >  1:6372389/1‑150 (MQ=255)
                 aaaGCGCCGCGCAATTGGTCGCGGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTcc                                                                                                                       >  1:7955018/1‑150 (MQ=255)
                 aaaGCGCCGCGCAATTGGTCGCGGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTcc                                                                                                                       <  1:5773436/150‑1 (MQ=255)
                  aaGCGCCGCGCAATTGGTCGCGGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTccc                                                                                                                      <  2:4593495/150‑1 (MQ=255)
                     cgccgcGCACTTGGGCGCGGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGGGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGACGTGGTCGCGCTGGTCTGCCTGGAAAGCGCTGTCCCgga                                                                                                                   <  2:10397723/150‑3 (MQ=255)
                     cgccgcGCAATTGGTCGCGGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCgga                                                                                                                   <  2:8245139/150‑3 (MQ=255)
                     cgccgcGCAAGTGGTCGCGGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCgga                                                                                                                   <  2:2877209/150‑3 (MQ=255)
                      gccgcGCAATTGGTCGCGGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGAc                                                                                                                  >  2:3482312/1‑150 (MQ=255)
                       ccgcGCAATTGGTCGCGGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACg                                                                                                                 >  1:4062786/1‑150 (MQ=255)
                        cgcgCAATTGGTCGCGGTGATCGGAATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGc                                                                                                                <  2:6882281/150‑1 (MQ=255)
                            cAATTGGTCGCGGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGc                                                                                                            >  2:2700002/1‑150 (MQ=255)
                            cAATTGGTCGCGGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGc                                                                                                            <  2:10159329/150‑1 (MQ=255)
                             aaTTGGTCGCGGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATTTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCt                                                                                                           >  2:8483763/1‑150 (MQ=255)
                             aaTTGGTCGCGGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCGGCGGCGCCAGGCGCTCCTCGCCAGCGCCGAAGTGGTCGCGGTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCt                                                                                                           >  2:7829548/1‑150 (MQ=255)
                               ttGGTCGCGGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTgc                                                                                                         >  2:7543262/1‑150 (MQ=255)
                                      cgGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCg                                                                                                  >  1:9953547/1‑150 (MQ=255)
                                        gTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTg                                                                                                >  1:1790542/1‑150 (MQ=255)
                                         tGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTgg                                                                                               >  1:9572997/1‑150 (MQ=255)
                                          gATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGc                                                                                              <  2:4108670/150‑1 (MQ=255)
                                             cGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGTCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCAt                                                                                           >  1:6837373/1‑150 (MQ=255)
                                             cGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCAt                                                                                           <  2:2320526/150‑1 (MQ=255)
                                             cGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCAt                                                                                           <  1:6615456/150‑1 (MQ=255)
                                                cATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACCTCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGa                                                                                        <  1:1277903/150‑1 (MQ=255)
                                                cATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGa                                                                                        <  2:8335784/150‑1 (MQ=255)
                                                 aTCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGAc                                                                                       <  1:2992984/150‑1 (MQ=255)
                                                     tccAGGCCGGCGCAGCCTATGTGCCGGTGGACCTCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGc                                                                                   <  1:8277864/150‑1 (MQ=255)
                                                      ccAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGACTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCt                                                                                  >  1:10542705/1‑150 (MQ=255)
                                                       cAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCTCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTg                                                                                 >  2:4670912/1‑150 (MQ=255)
                                                         ggCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGgc                                                                               <  1:6737643/150‑1 (MQ=255)
                                                           ccGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGgccg                                                                             >  2:3292444/1‑150 (MQ=255)
                                                            cGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGgccgc                                                                            <  2:10678820/150‑1 (MQ=255)
                                                             ggCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGgccgcc                                                                           <  2:9781427/150‑1 (MQ=255)
                                                                gcAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGAc                                                                        >  1:8455316/1‑150 (MQ=255)
                                                                  aGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAg                                                                      <  2:9694353/150‑1 (MQ=255)
                                                                   gCCGATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAgc                                                                     >  1:4964135/1‑150 (MQ=255)
                                                                      tATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGcc                                                                  >  1:4016010/1‑150 (MQ=255)
                                                                           gCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGACGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGgcc                                                             >  1:7077951/1‑150 (MQ=255)
                                                                              ggTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGgccgcc                                                          <  2:9380486/150‑1 (MQ=255)
                                                                               gTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCa                                                         >  2:9291027/1‑150 (MQ=255)
                                                                                 ggACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCAcc                                                       <  1:3569181/150‑1 (MQ=255)
                                                                                 ggACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCAcc                                                       >  2:5574871/1‑150 (MQ=255)
                                                                                  gACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACCGCGCCTGGCCGCCAccg                                                      >  2:10503519/1‑150 (MQ=255)
                                                                                    cctCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCAccgcc                                                    <  2:8469239/148‑1 (MQ=255)
                                                                                      tCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCAccgcccg                                                  >  1:2458345/1‑150 (MQ=255)
                                                                                       ccGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCATGGCCGCCAccgcccgc                                                 >  2:2225455/1‑150 (MQ=255)
                                                                                        cGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCcgcg                                                <  2:374982/150‑1 (MQ=255)
                                                                                        cGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCcgcg                                                >  2:10041005/1‑150 (MQ=255)
                                                                                           cAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCgcggcg                                             >  1:8924083/1‑150 (MQ=255)
                                                                                           cAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCgcggcg                                             <  2:5697774/150‑1 (MQ=255)
                                                                                            aGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGcggcgg                                            <  1:379407/150‑1 (MQ=255)
                                                                                             gccgccGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGa                                           >  2:2508421/1‑150 (MQ=255)
                                                                                             gccgccGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGa                                           >  1:3118480/1‑150 (MQ=255)
                                                                                             gccgccGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGa                                           >  2:2945808/1‑150 (MQ=255)
                                                                                             gccgccGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGa                                           <  2:10328276/150‑1 (MQ=255)
                                                                                              ccgccgCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAg                                          <  1:5888029/150‑1 (MQ=255)
                                                                                              ccgccgCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAg                                          >  1:8717249/1‑150 (MQ=255)
                                                                                               cgccgcTGCGGCGCCATGCGACCCTCGCCAGCGCCTATGTGGTCGCGGTGGTCTGCCTGGACAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAgg                                         <  1:25610/150‑1 (MQ=255)
                                                                                               cgccgcTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAgg                                         >  1:2188953/1‑150 (MQ=255)
                                                                                               cgccgcTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAgg                                         <  1:5383604/150‑1 (MQ=255)
                                                                                                gccgcTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGt                                        <  1:4753903/150‑1 (MQ=255)
                                                                                                 ccgcTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGCAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTg                                       >  1:422277/1‑150 (MQ=255)
                                                                                                 ccgcTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTg                                       >  2:10614258/1‑150 (MQ=255)
                                                                                                    cTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTggcg                                    >  1:7322417/1‑150 (MQ=255)
                                                                                                     tGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTggcgg                                   <  2:1883196/150‑1 (MQ=255)
                                                                                                     tGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTggcgg                                   <  1:2075602/150‑1 (MQ=255)
                                                                                                     tGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCAGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTggcgg                                   <  1:6182309/150‑1 (MQ=255)
                                                                                                       cggcgCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTGgcggcg                                 <  1:7778885/150‑1 (MQ=255)
                                                                                                       cggcgCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTGgcggcg                                 >  1:625797/1‑150 (MQ=255)
                                                                                                         gcgcCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTGGCGGCGga                               <  1:1490220/150‑1 (MQ=255)
                                                                                                          cgcCAGGCGATCCTCGCCAGCGCCGACGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTGGCGGCGgac                              <  1:3359307/150‑1 (MQ=255)
                                                                                                            ccAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTGGCGGCGgacga                            >  1:8060972/1‑150 (MQ=255)
                                                                                                              aGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTGGCGGCGGACGAcc                          >  1:9789327/1‑150 (MQ=255)
                                                                                                               ggCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTGGCGGCGGACGACCt                         >  2:4854509/1‑150 (MQ=255)
                                                                                                                  gATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTGGCGGCGGACGACCTCGc                      >  2:6363193/1‑150 (MQ=255)
                                                                                                                   aTCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTGGCGGCGGACGACCTCGcc                     >  1:9483335/1‑150 (MQ=255)
                                                                                                                   aTCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTGGCGGCGGACGACCTCGcc                     >  2:7189687/1‑150 (MQ=255)
                                                                                                                      cTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTGGCGGCGGACGACCTCGCCTAc                  >  1:6039727/1‑150 (MQ=255)
                                                                                                                         gCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTGGCGGCGGACGACCTCGCCTACGTg               >  1:5307810/1‑150 (MQ=255)
                                                                                                                          ccAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACCGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTGGCGGCGGACGACCTCGCCTACGTGa              >  2:6645158/1‑150 (MQ=255)
                                                                                                                           cAGCGCCGCAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTGGCGGCGGACGACCTCGCCTACGTGAt             <  2:1629265/150‑1 (MQ=255)
                                                                                                                             gcgccGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTGGCGGCGGACGACCTCGCCTACGTGATCt           <  2:8959245/147‑1 (MQ=255)
                                                                                                                              cgccGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTGGCGGCGGACGACCTCGCCTACGTGATCTa          >  2:8637602/3‑150 (MQ=255)
                                                                                                                                ccGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTGGCGGCGGACGACCTCGCCTACGTGATCTaca        >  2:1506582/1‑150 (MQ=255)
                                                                                                                                ccGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTGGCGGCGGACGACCTCGCCTACGTGATCTaca        <  1:5129094/150‑1 (MQ=255)
                                                                                                                                 cGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTGGCGGCGGACGACCTCGCCTACGTGATCTacac       >  1:1636515/1‑150 (MQ=255)
                                                                                                                                 cGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTGGCGGCGGACGACCTCGCCTACGTGATCTacac       >  1:9061573/1‑150 (MQ=255)
                                                                                                                                  gAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTGGCGGCGGACGACCTCGCCTACGTGATCTACAcc      <  1:7555641/150‑1 (MQ=255)
                                                                                                                                   aaGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCAATCGACAGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTGGCGGCGGACGACCTCGCCTGCGGGATCTGCACCt     >  2:3040091/1‑150 (MQ=255)
                                                                                                                                    aGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTGGCGGCGGACGACCTCGCCTACGTGATCTACACCTc    <  1:2351758/150‑1 (MQ=255)
                                                                                                                                      tGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTGGCGGCGGACGACCTCGCCTACGTGATCTACACCTCCg  <  1:10052954/150‑1 (MQ=255)
                                                                                                                                      tGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCGGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTGGCGGCGGACGACCTCGCCTACGTGATCTACACCTCCg  <  1:273362/150‑1 (MQ=255)
                                                                                                                                             |||                                                                                                                                            
CGCGGTGATGCTGCCGAAAAGCGCCGCGCAATTGGTCGCGGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCCCCGAAGTGGTCGCCTGGGTCTGCCTGGAAAGCGATGTCCCGAACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTGGCGGCGGACGACCTCGCCTACGTGATCTACACCTCCG  >  NC_008463/791721‑792004

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑