Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 888,038 2 bp→TG coding (1562‑1563/1878 nt) PA14_RS04165 → sigma‑54‑dependent Fis family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_008463888,0380CT99.8% 2213.7 / NA 523P521M (CCT→CTG) PA14_RS04165sigma‑54‑dependent Fis family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (261/261);  minor base G (0/1);  total (261/262)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*NC_008463888,0390TG99.8% 2077.8 / NA 523P521M (CCT→CTG) PA14_RS04165sigma‑54‑dependent Fis family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base G (261/261);  minor base C (0/1);  total (261/262)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 523 total aligned reads displayed.

AGGATCTCTACTTCCGCCTCGGCGGCGCCCGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCCTGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGACCTTCCGGCAGAGCTGCGTGGCGAAGGCCGGACACCGGC  >  NC_008463/887894‑888182
                                                                                                                                                ||                                                                                                                                               
 ggATCTCTACTTCCGCCTCGGCGGCGCCCGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATg                                                                                                                                            >  1:9440548/1‑150 (MQ=255)
 ggATCTCTACTTCCGCCTCGGCGGCGCCCGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATg                                                                                                                                            >  2:1164183/1‑150 (MQ=255)
 ggATCTCTACTTCCGCCTCGGCGGCGCCCGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATg                                                                                                                                            >  1:4255067/1‑150 (MQ=255)
     ctctACTTCCGCCTCGGCGGCGCCCGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTg                                                                                                                                        >  1:4983663/1‑150 (MQ=255)
      tctACTTCCGCCTCGGCGGCGCCCGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGc                                                                                                                                       <  2:9847225/150‑1 (MQ=255)
      tctACTTCCGCCTCGGCGGCGCCCGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGc                                                                                                                                       >  2:3008624/1‑150 (MQ=255)
         aCTTCCGCCTCGGCGGCGCCCGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCg                                                                                                                                    <  1:8488529/150‑1 (MQ=255)
         aCTTCCGCCTCGGCGGCGCCCGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCg                                                                                                                                    <  2:7675477/150‑1 (MQ=255)
          cTTCCGCCTCGGCGGCGCCCGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCgg                                                                                                                                   <  2:6293131/150‑1 (MQ=255)
          cTTCCGCCTCGGCGGCGCCCGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCgg                                                                                                                                   >  2:10420774/1‑150 (MQ=255)
           ttCCGCCTCGGCGGCGCCCGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGc                                                                                                                                  <  2:3850753/150‑1 (MQ=255)
            tCCGCCTCGGCGGCGCCCGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCt                                                                                                                                 <  1:2995691/150‑1 (MQ=255)
             ccGCCTCGGCGGCGCCCGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTa                                                                                                                                <  2:5374071/150‑1 (MQ=255)
             ccGCCTCGGCGGCGCCCGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTa                                                                                                                                <  2:7327403/150‑1 (MQ=255)
                 cTCGGCGGCGCCCGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGc                                                                                                                            <  1:389161/150‑1 (MQ=255)
                 cTCGGCGGCGCCCGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGc                                                                                                                            >  2:2878475/1‑150 (MQ=255)
                  tCGGCGGCGCCCGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGCGGCAGCCCTGGAATGCCTGCTCGGCTATCGCt                                                                                                                           >  2:4273751/1‑150 (MQ=255)
                  tCGGCGGCGCCCGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCt                                                                                                                           >  1:3523966/1‑150 (MQ=255)
                   cggcggCGCCCGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTg                                                                                                                          >  2:3766006/1‑150 (MQ=255)
                    ggcggcGCCCGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTgg                                                                                                                         <  1:9691606/150‑1 (MQ=255)
                    ggcggcGCCCGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTgg                                                                                                                         >  2:5791077/1‑150 (MQ=255)
                     gcggcgCCCGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGc                                                                                                                        <  2:540536/150‑1 (MQ=255)
                     gcggcgCCCGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGc                                                                                                                        <  1:1338291/150‑1 (MQ=255)
                       ggcgCCCGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCg                                                                                                                      <  1:9153306/150‑1 (MQ=255)
                        gcgcCCGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCgg                                                                                                                     >  1:5977503/1‑150 (MQ=255)
                           cccGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCa                                                                                                                  <  2:1471709/150‑1 (MQ=255)
                           cccGCGTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCa                                                                                                                  <  2:6469690/150‑1 (MQ=255)
                               cTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCTCTGGCCGGGCAATGt                                                                                                              >  2:5497892/1‑150 (MQ=255)
                                    aaCTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCCATGTCCGcc                                                                                                         >  2:3973162/1‑150 (MQ=255)
                                    aaCTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGcc                                                                                                         <  2:10711204/150‑1 (MQ=255)
                                       tGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGt                                                                                                      <  2:9352900/150‑1 (MQ=255)
                                         ccgccgCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTg                                                                                                    <  2:1920604/150‑1 (MQ=255)
                                           gccgcTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCg                                                                                                  >  2:10250262/1‑150 (MQ=255)
                                              gcTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCa                                                                                               >  1:8670873/1‑150 (MQ=255)
                                              gcTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCa                                                                                               <  1:8194699/150‑1 (MQ=255)
                                                tACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCAtg                                                                                             >  1:7648336/1‑150 (MQ=255)
                                                tACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCAtg                                                                                             >  1:7981513/1‑150 (MQ=255)
                                                    cgAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGtt                                                                                         <  2:9032269/150‑1 (MQ=255)
                                                    cgAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGAACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGtt                                                                                         >  2:2187140/1‑150 (MQ=255)
                                                     gAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTg                                                                                        <  2:7830532/150‑1 (MQ=255)
                                                     gAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTg                                                                                        <  1:6825071/150‑1 (MQ=255)
                                                         gCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCt                                                                                    <  1:6418023/150‑1 (MQ=255)
                                                            gCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCAGGTGTTGCGCTACg                                                                                 >  2:1689931/1‑150 (MQ=255)
                                                                 cGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTgc                                                                            <  2:2325305/150‑1 (MQ=255)
                                                                 cGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTgc                                                                            <  2:7066628/150‑1 (MQ=255)
                                                                  ggCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTgcg                                                                           >  2:6745513/1‑150 (MQ=255)
                                                                      ggCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCt                                                                       >  1:4618446/1‑150 (MQ=255)
                                                                      ggCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCt                                                                       >  1:8307256/1‑150 (MQ=255)
                                                                          cTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTgc                                                                   <  2:10035158/150‑1 (MQ=255)
                                                                          cTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTgc                                                                   >  1:2669829/1‑150 (MQ=255)
                                                                          cTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTgc                                                                   >  1:1250443/1‑150 (MQ=255)
                                                                           tGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTgcg                                                                  <  1:2826288/150‑1 (MQ=255)
                                                                            gATCCGGCGCATCCTCGACGAGGAATCCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTCCGCCTGCGCGCTGTgcgg                                                                 <  2:753786/150‑1 (MQ=255)
                                                                            gATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTgcgg                                                                 >  1:5490682/1‑150 (MQ=255)
                                                                             aTCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTgcggc                                                                <  2:3060696/150‑1 (MQ=255)
                                                                             aTCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTgcggc                                                                >  2:3324837/1‑150 (MQ=255)
                                                                              tCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTgcggcg                                                               <  2:8572403/150‑1 (MQ=255)
                                                                               ccGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTgcggcgg                                                              <  2:9216268/150‑1 (MQ=255)
                                                                                cGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTgcggcggc                                                             <  1:1972800/150‑1 (MQ=255)
                                                                                cGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTgcggcggc                                                             <  2:4653308/150‑1 (MQ=255)
                                                                                  gcgcATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGgcgc                                                           <  2:7036586/150‑1 (MQ=255)
                                                                                    gcATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCa                                                         <  2:3531779/150‑1 (MQ=255)
                                                                                     cATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCAc                                                        >  1:2333415/1‑150 (MQ=255)
                                                                                       tCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCAccc                                                      >  1:2129855/1‑150 (MQ=255)
                                                                                             acgaGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAAc                                                <  1:10315145/150‑1 (MQ=255)
                                                                                              cgaGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACt                                               >  2:605516/1‑150 (MQ=255)
                                                                                               gaGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTc                                              <  1:5221134/150‑1 (MQ=255)
                                                                                                aGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCg                                             <  1:1600813/150‑1 (MQ=255)
                                                                                                aGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCg                                             <  2:842721/150‑1 (MQ=255)
                                                                                                  gAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGcc                                           <  1:776915/150‑1 (MQ=255)
                                                                                                      ccGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGAcc                                       <  2:4152386/150‑1 (MQ=255)
                                                                                                      ccGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGAcc                                       >  1:659021/1‑150 (MQ=255)
                                                                                                        gcgcACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGACCtt                                     <  1:8858652/150‑1 (MQ=255)
                                                                                                        gcgcACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGACCtt                                     <  1:10083611/150‑1 (MQ=255)
                                                                                                         cgcACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGACCTTc                                    >  1:7819847/1‑150 (MQ=255)
                                                                                                         cgcACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCAGGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGACCTTc                                    >  2:8207609/1‑150 (MQ=255)
                                                                                                             cTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGACCTTCCGGc                                <  2:4567667/150‑1 (MQ=255)
                                                                                                               gcggcgTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGACCTTCCGGCag                              <  1:7235466/150‑1 (MQ=255)
                                                                                                                 ggcgTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGACCTTCCGGCagag                            >  1:3145439/1‑150 (MQ=255)
                                                                                                                  gcgTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGACCTTCCGGCAGAGc                           >  1:6209091/1‑150 (MQ=255)
                                                                                                                  gcgTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGACCTTCCGGCAGAGc                           <  2:2475315/150‑1 (MQ=255)
                                                                                                                    gTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGACCTTCCGGCAGAGCTg                         >  2:8039834/1‑150 (MQ=255)
                                                                                                                      aCGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGACCTTCCGGCAGAGCTGCg                       <  1:8800174/150‑1 (MQ=255)
                                                                                                                      aCGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGACCTTCCGGCAGAGCTGCg                       >  2:5103086/1‑150 (MQ=255)
                                                                                                                       cGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGACCTTCCGGCAGAGCTGCGt                      >  1:6073406/1‑150 (MQ=255)
                                                                                                                         cATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGACCTTCCGGCAGAGCTGCGTgg                    >  2:6189257/1‑150 (MQ=255)
                                                                                                                         cATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGACCTTCCGGCAGAGCTGCGTgg                    <  1:9305418/150‑1 (MQ=255)
                                                                                                                          aTCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGACCTTCCGGCAGAGCTGCGTGGc                   >  1:4891856/1‑150 (MQ=255)
                                                                                                                          aTCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGACCTTCCGGCAGAGCTGCGTGGc                   <  1:2734261/150‑1 (MQ=255)
                                                                                                                            cGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGACCTTCCGGCAGAGCTGCGTGGCGa                 <  1:7372553/150‑1 (MQ=255)
                                                                                                                             gAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGACCTTCCGGCAGAGCTGCGTGGCGaa                >  2:7221077/1‑150 (MQ=255)
                                                                                                                               aCTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGACCTTCCGGCAGAGCTGCGTGGCGAAgg              >  1:10657959/1‑150 (MQ=255)
                                                                                                                                cTCGGCGAGGCAGCCCTGGACTGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGACCTTCCGGCAGAGCTGCGTGGCGAAGGc             <  1:4508434/150‑1 (MQ=255)
                                                                                                                                cTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGACCTTCCGGCAGAGCTGCGTGGCGAAGGc             >  1:5742031/1‑150 (MQ=255)
                                                                                                                                 tCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCGGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGACCTTCCGGCAGAGCTGCGTGGCGAAGGcc            <  2:9496804/150‑1 (MQ=255)
                                                                                                                                   ggCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGACCTTCCGGCAGAGCTGCGTGGCGAAGGCCgg          >  2:7107528/1‑150 (MQ=255)
                                                                                                                                     cGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGACCTTCCGGCAGAGCTGCGTGGCGAAGGCCGGac        >  1:6958908/1‑150 (MQ=255)
                                                                                                                                      gAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGACCTTCCGGCAGAGCTGCGTGGCGAAGGCCGGacc       >  1:7580070/1‑149 (MQ=255)
                                                                                                                                        ggCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGACCTTCCGGCAGAGCTGCGTGGCGAAGGCCGGACAcc     <  1:9878336/150‑1 (MQ=255)
                                                                                                                                           aGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGACCTTCCGGCAGAGCTGCGTGGCGAAGGCCGGACACCGGc  >  2:128179/1‑150 (MQ=255)
                                                                                                                                                ||                                                                                                                                               
AGGATCTCTACTTCCGCCTCGGCGGCGCCCGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCCTGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGACCTTCCGGCAGAGCTGCGTGGCGAAGGCCGGACACCGGC  >  NC_008463/887894‑888182

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑