BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_008463	3,670,537	Δ1 bp	coding (537/1020 nt)	PA14_RS16675 →	ABC transporter permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_008463	3,670,534	0	T	.	99.0%	1889.0 / 7.4	389	coding (534/1020 nt)	PA14_RS16675	ABC transporter permease
Reads supporting (aligned to +/- strand): ref base T (2/2); new base . (184/201); total (186/203)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 400 total aligned reads displayed.

AGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCGTTTTCCTGG‑A‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTCCGCGCCGAGTTCC  >  NC_008463/3670384‑3670679

ggCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCGt                                                                                                                                                     <  2:1633977/150‑1 (MQ=255)

 gCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCGtt                                                                                                                                                    <  2:10008541/151‑1 (MQ=255)

   ccGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCGtttc                                                                                                                                                  <  1:8891060/151‑2 (MQ=255)

   ccGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCGtttc                                                                                                                                                  >  2:9457343/1‑150 (MQ=255)

       gACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTgg                                                                                                                                             >  1:2793997/1‑151 (MQ=255)

        aCGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGG‑a                                                                                                                                           >  1:11013294/1‑151 (MQ=255)

        aCGTGCTCGACCGGGTGATTTACTGCTTCCGCGTGTCGATCCTCTTCGCCCTGAACCTGACCGAGCTCAGTTATCTGATCTGCGTCACCGTCGGCGCCCTGCAAGGATAATACTGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGG‑a                                                                                                                                           >  2:6141291/1‑151 (MQ=255)

         cGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGCCCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACGACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGG‑aa                                                                                                                                          <  2:3492714/151‑2 (MQ=255)

         cGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGG‑aa                                                                                                                                          >  1:4909788/1‑150 (MQ=255)

          gTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCAACTACGGCGGCACGGTCGACCTGCTCGGCCAGCG‑TTTCCTGG‑aag                                                                                                                                         >  2:3295847/1‑149 (MQ=255)

            gCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGG‑aagtc                                                                                                                                       <  1:1423296/151‑5 (MQ=255)

             cTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGG‑aagtct                                                                                                                                      <  1:9111859/151‑6 (MQ=255)

              tCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTg                                                                                                                                     <  1:3981039/151‑1 (MQ=255)

               cGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTgg                                                                                                                                    <  1:5105861/151‑1 (MQ=255)

                 cccGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTc                                                                                                                                  >  1:6032061/1‑151 (MQ=255)

                  ccGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCg                                                                                                                                 <  1:3316342/151‑1 (MQ=255)

                  ccGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCg                                                                                                                                 >  2:5998675/1‑151 (MQ=255)

                     ggTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCgggg                                                                                                                              <  1:6683961/151‑1 (MQ=255)

                        gATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTg                                                                                                                           <  1:7676694/151‑1 (MQ=255)

                           ttACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCg                                                                                                                        <  2:6980374/151‑1 (MQ=255)

                             aCGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGt                                                                                                                      >  1:480274/1‑151 (MQ=255)

                              cGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTg                                                                                                                     <  2:2224325/151‑1 (MQ=255)

                                  ttCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGctct                                                                                                                 >  1:7096216/1‑151 (MQ=255)

                                  ttCCGCGTGTCGATCATCTTCGCCCTGACACTGACCGAGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCGGTGGAAGGCTAGTACGGCGGCTTGGTCGAGCTGCTCGGGCGGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGctcg                                                                                                                 >  2:3961385/1‑150 (MQ=255)

                                   tCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTa                                                                                                                <  2:8438062/151‑1 (MQ=255)

                                      gcgTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACct                                                                                                             <  1:10015023/151‑1 (MQ=255)

                                       cgTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACctg                                                                                                            <  1:1552225/151‑1 (MQ=255)

                                        gtgtCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACctgc                                                                                                           >  1:325355/1‑151 (MQ=255)

                                         tgtCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACctgct                                                                                                          >  2:3217898/1‑151 (MQ=255)

                                          gtCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACctgctg                                                                                                         <  2:5114710/151‑1 (MQ=255)

                                            cGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGat                                                                                                       >  1:7838985/1‑151 (MQ=255)

                                            cGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGat                                                                                                       >  2:4534349/1‑151 (MQ=255)

                                             gATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGatc                                                                                                      >  1:9592544/1‑151 (MQ=255)

                                              aTCCTCTTCGCCGTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCGTGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGatca                                                                                                     <  2:6379188/151‑1 (MQ=255)

                                               tCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGatcat                                                                                                    <  2:4960526/151‑1 (MQ=255)

                                               tCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGatcat                                                                                                    >  1:10813651/1‑151 (MQ=255)

                                               tCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGatcat                                                                                                    <  2:1511568/151‑1 (MQ=255)

                                                 ctctTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATcc                                                                                                  >  1:7402112/1‑151 (MQ=255)

                                                     tCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGc                                                                                              >  1:10353401/1‑151 (MQ=255)

                                                     tCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCAGCCTGGc                                                                                              >  2:10507250/1‑151 (MQ=255)

                                                        ccctgaccctgaCCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAg                                                                                           >  2:3397701/1‑151 (MQ=255)

                                                        ccctgaccctgaCCGTGCTCAGTTCACTGATCGGCGTCTCCGTCGGAGCCCTGCAAGGATACTACGTCGTCTGGGTCGACCTGATCTGCCAGAG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCTGTGCTCTACCTGCTGATCATCCTGGCCAg                                                                                           <  2:6737372/151‑1 (MQ=255)

                                                            gaccctgaccGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTc                                                                                       >  1:5549299/1‑151 (MQ=255)

                                                              ccctgaccGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGt                                                                                     <  1:8518497/151‑1 (MQ=255)

                                                                ctgaccGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTcc                                                                                   <  1:10664499/151‑1 (MQ=255)

                                                                ctgaccGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCGCGGCAAGC‑TTTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGTTCATCCTGGCCAGCTTCGTcc                                                                                   >  2:2657224/1‑151 (MQ=255)

                                                                   accGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGc                                                                                >  2:2959295/1‑151 (MQ=255)

                                                                       tGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGaa                                                                            <  1:11077503/151‑1 (MQ=255)

                                                                            aGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCt                                                                       >  1:10867581/1‑151 (MQ=255)

                                                                            aGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCt                                                                       <  1:11226011/151‑1 (MQ=255)

                                                                            aGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCGTCGTCCAGCCGAACTTCt                                                                       >  2:3049493/1‑151 (MQ=255)

                                                                             gTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCtg                                                                      <  1:5669672/151‑1 (MQ=255)

                                                                              ttCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCtgg                                                                     <  1:6263851/151‑1 (MQ=255)

                                                                               tctcTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCtggt                                                                    <  2:3744794/151‑1 (MQ=255)

                                                                                   tGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGgct                                                                <  2:7299190/151‑1 (MQ=255)

                                                                                     aTCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGgctgc                                                              >  2:8640886/1‑151 (MQ=255)

                                                                                      tCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGgctgct                                                             <  1:6107642/151‑1 (MQ=255)

                                                                                       cGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGctgctg                                                            >  2:6121702/1‑151 (MQ=255)

                                                                                       cGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGctgctg                                                            >  2:530831/1‑151 (MQ=255)

                                                                                        ggCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTgg                                                           >  2:1943363/1‑151 (MQ=255)

                                                                                        ggCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGG‑ACGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAGCTGCTGGTGGCTGCTgg                                                           >  2:9646638/1‑151 (MQ=255)

                                                                                          cGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGc                                                         <  2:10236719/151‑1 (MQ=255)

                                                                                            tCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGcat                                                       <  2:2781112/151‑1 (MQ=255)

                                                                                              aCCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGcatca                                                     <  1:2658967/151‑1 (MQ=255)

                                                                                              aCCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGcatca                                                     <  1:8278209/151‑1 (MQ=255)

                                                                                              aCCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGcatca                                                     >  1:8027245/1‑151 (MQ=255)

                                                                                               ccGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGcatcat                                                    >  2:7814184/1‑151 (MQ=255)

                                                                                                cGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCAtg                                                   >  1:5433356/1‑151 (MQ=255)

                                                                                                cGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCAtg                                                   >  2:8045572/1‑151 (MQ=255)

                                                                                                   cGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCAtgctg                                                >  2:958250/1‑151 (MQ=255)

                                                                                                     gcgcCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATgctgct                                              <  1:3617472/151‑1 (MQ=255)

                                                                                                       gcCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGt                                            >  1:10541655/1‑151 (MQ=255)

                                                                                                         ccTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGttt                                          <  2:9061984/151‑1 (MQ=255)

                                                                                                         ccTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGttt                                          <  1:1987065/151‑1 (MQ=255)

                                                                                                           tGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTc                                        <  2:6476947/151‑1 (MQ=255)

                                                                                                            gCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTcc                                       <  1:10459298/151‑1 (MQ=255)

                                                                                                               aGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTgg                                    >  2:10860511/1‑151 (MQ=255)

                                                                                                                 gCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGAt                                  <  1:3496196/151‑1 (MQ=255)

                                                                                                                   tactacGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGa                                >  1:8816581/1‑151 (MQ=255)

                                                                                                                   tactacGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGa                                <  1:2206751/151‑1 (MQ=255)

                                                                                                                    actacGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAg                               <  1:371425/151‑1 (MQ=255)

                                                                                                                    actacGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTGCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAg                               >  2:1728819/1‑151 (MQ=255)

                                                                                                                     ctacGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGc                              <  1:9307668/151‑1 (MQ=255)

                                                                                                                      tacGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGcc                             >  1:2936709/1‑151 (MQ=255)

                                                                                                                       acGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCt                            <  2:11090439/151‑1 (MQ=255)

                                                                                                                         ggcggcTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCtgg                          <  2:8328843/151‑1 (MQ=255)

                                                                                                                          gcggcTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCtggt                         <  1:4359759/151‑1 (MQ=255)

                                                                                                                              cTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGAc                     >  1:6421428/1‑151 (MQ=255)

                                                                                                                                gggTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGt                   <  2:576074/151‑1 (MQ=255)

                                                                                                                                  gTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTgg                 >  1:9881101/1‑151 (MQ=255)

                                                                                                                                  gTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTgg                 >  1:9019445/1‑151 (MQ=255)

                                                                                                                                     gACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTcc              <  2:1259772/151‑1 (MQ=255)

                                                                                                                                       ccTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTCcgc            >  1:6968016/1‑151 (MQ=255)

                                                                                                                                         tGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTCCgcgc          >  2:4795578/1‑151 (MQ=255)

                                                                                                                                          gCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTCCGCGcc         <  1:2417375/151‑1 (MQ=255)

                                                                                                                                          gCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTCCGCGcc         <  1:6002522/151‑1 (MQ=255)

                                                                                                                                              ggCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTCCGCGCCGAGt     >  2:4117309/1‑151 (MQ=255)

                                                                                                                                              ggCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTCCGCGCCGAGt     <  2:10717903/151‑1 (MQ=255)

                                                                                                                                               gCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTCCGCGCCGAGtt    <  1:585735/151‑1 (MQ=255)

                                                                                                                                                ccAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTCCGCGCCGAGTtc   >  2:4230497/1‑151 (MQ=255)

AGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCGTTTTCCTGG‑A‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTCCGCGCCGAGTTCC  >  NC_008463/3670384‑3670679

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`