Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 4,700,807 Δ1 bp intergenic (+165/‑150) PA14_RS21585 → / → pbpG aspartate/tyrosine/aromatic aminotransferase/D‑alanyl‑D‑alanine endopeptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084634,700,8070G.100.0% 2341.2 / NA 471intergenic (+165/‑150)PA14_RS21585/pbpGaspartate/tyrosine/aromatic aminotransferase/D‑alanyl‑D‑alanine endopeptidase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (220/251);  total (220/251)

Only 100 of 471 total aligned reads displayed.

AGCCGGCTGTGATGGCCGGCTTTTTTTCGCCCCAGCGAATGCCGCGCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGCGCGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCACCAGTTCACACTGCGCCCCACGGCCCCCACTTTCTCGTCTTAGTGAGCAAC  >  NC_008463/4700661‑4700952
                                                                                                                                                  |                                                                                                                                                 
aGCCGGCTGTGATGGCCGGCTTTTTTTCGCCCCAGCGAATGCCGCGCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAAc                                                                                                                                              >  2:2880449/1‑151 (MQ=255)
  ccGGCTGTGATGGCCGGCTTTTTTTCGCCCCAGCGAATGCCGCGCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACct                                                                                                                                            >  1:8266548/1‑151 (MQ=255)
    ggCTGTGATGGCCGGCTTTTTTTCGCCCCAGCGAATGCCGCGCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACctct                                                                                                                                          <  1:9195542/151‑1 (MQ=255)
      cTGTGATGGCCGGCTTTTTTTCGCCCCAGCGAATGCCGCGCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTcc                                                                                                                                        <  2:8645207/151‑1 (MQ=255)
        gtgATGGCCGGCTTTTTTTCGCCCCAGCGAATGCCGCGCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGa                                                                                                                                      >  1:5114808/1‑151 (MQ=255)
        gtgATGGCCGGCTTTTTTTCGCCCCAGCGAATGCCGCGCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGa                                                                                                                                      >  2:709906/1‑151 (MQ=255)
        gtgATGGCCGGCTTTTTTTCGCCCCAGCGAATGCCGCGCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGa                                                                                                                                      <  1:2544344/151‑1 (MQ=255)
          gATGGCCGGCTTTTTTTCGCCCCGGCGAATGCCGCGCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAAc                                                                                                                                    <  1:10021764/151‑1 (MQ=255)
           aTGGCCGGCTTTTTTTCGCCCCAGCGAATGCCGCGCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAAcc                                                                                                                                   <  2:8938470/151‑1 (MQ=255)
             ggCCGGCTTTTTTTCGCCCCAGCGAATGCCGCGCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGt                                                                                                                                 <  1:854321/151‑1 (MQ=255)
             ggCCGGCTTTTTTTCGCCCCAGCGAATGCCGCGCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGt                                                                                                                                 <  1:213688/151‑1 (MQ=255)
             ggCCGGCTTTTTTTCGCCCCAGCGAATGCCGCGCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGt                                                                                                                                 <  1:2191588/151‑1 (MQ=255)
               ccGGCTTTTTTTCGCCCCAGCGAATGCCGCGCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGc                                                                                                                               >  1:7707341/1‑151 (MQ=255)
                   cTTTTTTTCGCCCCAGCGAATGCCGCGCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTGTCCGC‑CGTACCTCTCCGAACCGTGcctag                                                                                                                           >  1:10445625/1‑148 (MQ=255)
                   cTTTTTTTCGCCCCAGCGAATGCCGCGCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAAc                                                                                                                           <  1:8033185/151‑1 (MQ=255)
                    tttttttCGCCCCAGCGAATGCCGCGCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCGCCGAACCGTGCCAccc                                                                                                                          >  1:9559619/1‑151 (MQ=255)
                        tttCGCCCCAGCGAATGCCGCGCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATaa                                                                                                                      <  1:3029925/151‑1 (MQ=255)
                            gCCCCAGCGAATGCCGCGCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCa                                                                                                                  >  2:5583563/1‑151 (MQ=255)
                               ccAGCGAATGCCGCGCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCa                                                                                                               >  1:3435737/1‑151 (MQ=255)
                               ccAGCGAATGCCGCGCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCa                                                                                                               <  1:11103319/151‑1 (MQ=255)
                                   cGAATGCCGCGCCCTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGACCCGCTCCGACCCGTGCCAACCATAAGTCAGCACgtt                                                                                                           >  2:8275289/1‑151 (MQ=38)
                                    gAATGCCGCGCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTc                                                                                                          <  2:9509409/151‑1 (MQ=255)
                                    gAATGCCGCGCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTc                                                                                                          >  1:7285836/1‑151 (MQ=255)
                                    gAATGCCGCGCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTc                                                                                                          >  2:7551753/1‑151 (MQ=255)
                                     aaTGCCGCGCTCTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCa                                                                                                         <  2:2104295/151‑1 (MQ=255)
                                           gcgcACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGccc                                                                                                   <  1:7431199/151‑1 (MQ=255)
                                           gcgcACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGccc                                                                                                   >  1:6774793/1‑151 (MQ=255)
                                            cgcCCTCCCGCCGCGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTGGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGACCCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCt                                                                                                  <  1:10370698/151‑1 (MQ=255)
                                              cACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTAc                                                                                                >  2:9202372/1‑151 (MQ=255)
                                              cACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTAc                                                                                                <  2:5367988/151‑1 (MQ=255)
                                               aCTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACg                                                                                               <  2:1869408/151‑1 (MQ=255)
                                               aCTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACg                                                                                               >  1:507178/1‑151 (MQ=255)
                                                 tCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTg                                                                                             <  1:452413/151‑1 (MQ=255)
                                                 tCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTg                                                                                             <  1:8808018/151‑1 (MQ=255)
                                                 tCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTg                                                                                             >  2:4806742/1‑151 (MQ=255)
                                                 tCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTg                                                                                             >  1:10828312/1‑151 (MQ=255)
                                                   ccgccgAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCt                                                                                           <  2:7918831/151‑1 (MQ=255)
                                                     gccgAGGCGGCGGATACCCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCttt                                                                                         <  2:11110659/151‑1 (MQ=255)
                                                     gccgAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCttt                                                                                         <  1:579742/151‑1 (MQ=255)
                                                        gAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTcc                                                                                      <  2:9329375/151‑1 (MQ=255)
                                                        gAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTcc                                                                                      <  1:389258/151‑1 (MQ=255)
                                                          ggcggcGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTa                                                                                    <  2:2446935/151‑1 (MQ=255)
                                                          ggcggcGGATAACCGCGTTGCGGGTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTa                                                                                    <  2:6154768/151‑1 (MQ=255)
                                                              gcggATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACaaa                                                                                <  1:2816868/151‑1 (MQ=255)
                                                                ggATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAag                                                                              >  2:10652856/1‑151 (MQ=255)
                                                                   tAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGc                                                                           <  1:3786108/151‑1 (MQ=255)
                                                                   tAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGc                                                                           <  1:2646259/151‑1 (MQ=255)
                                                                   tAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGc                                                                           >  1:10535764/1‑151 (MQ=255)
                                                                    aaCCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCa                                                                          <  1:10108688/151‑1 (MQ=255)
                                                                       cgcgTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCACCCATAAGTCAGCACTGTCAAAGCCCTACGGGCTGTTCCTACAAAAGAGCAGaa                                                                       >  2:8063987/1‑151 (MQ=255)
                                                                       cgcgTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATaa                                                                       >  1:10631737/1‑151 (MQ=255)
                                                                              cGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTc                                                                <  1:4940965/151‑1 (MQ=255)
                                                                               ggTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCa                                                               >  2:9798408/1‑151 (MQ=255)
                                                                                  tATTCGCCCTACCGCACCACGACCGTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAACGAGCATAAAATGCTCAAtt                                                            <  1:5539434/151‑1 (MQ=255)
                                                                                   aTTCGCCCTACCGCACCACCACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGTACCGTGCCAACCATAACTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAACAGCATAAACTGCTCAATTa                                                           <  2:5812780/151‑1 (MQ=255)
                                                                                     tCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATg                                                         <  2:8392298/151‑1 (MQ=255)
                                                                                     tCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATg                                                         <  1:3231782/151‑1 (MQ=255)
                                                                                      cGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATgg                                                        <  1:3526087/151‑1 (MQ=255)
                                                                                       gCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTCCAAAAGAGCATAAAATGCTCAATTATggg                                                       >  2:90552/1‑151 (MQ=255)
                                                                                       gCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATggg                                                       >  2:10068042/1‑151 (MQ=255)
                                                                                        cccTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGc                                                      >  1:3164373/1‑151 (MQ=255)
                                                                                         ccTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCa                                                     >  1:9796601/1‑151 (MQ=255)
                                                                                           tACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCAcc                                                   >  2:3816368/1‑151 (MQ=255)
                                                                                            aCCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCACCa                                                  <  1:2961024/151‑1 (MQ=255)
                                                                                             ccGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCACCAg                                                 >  1:6900061/1‑151 (MQ=255)
                                                                                             ccGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCACCAg                                                 <  1:4748226/151‑1 (MQ=255)
                                                                                             ccGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTATCAAAGCCCTACGTGCTATTCCTACAAAAGAGCATCATCTGCTCAATTATGGGCACCAg                                                 >  2:6242347/1‑151 (MQ=255)
                                                                                              cGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCACCAGt                                                >  2:4935595/1‑151 (MQ=255)
                                                                                               gCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGACCCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCACCAGtt                                               >  2:1467228/1‑151 (MQ=255)
                                                                                               gCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAATATGCTCAATTATGGGCACCAGtt                                               >  2:6800821/1‑151 (MQ=255)
                                                                                               gCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCACCAGtt                                               >  1:596797/1‑151 (MQ=255)
                                                                                                caccacGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCACCAGTTc                                              <  1:1096850/151‑1 (MQ=255)
                                                                                                   cacGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCACCAGTTcaca                                           <  1:1898100/151‑1 (MQ=255)
                                                                                                      gACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCACCAGTTCACACTg                                        <  1:1651105/151‑1 (MQ=255)
                                                                                                      gACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCACCAGTTCACACTg                                        >  1:866601/1‑151 (MQ=255)
                                                                                                           tgtgGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCACCAGTTCACACTGCGccc                                   <  1:1391628/151‑1 (MQ=255)
                                                                                                              gggCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGACCCGTCCCAACCAGACCTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAACGAGCATAAAATGCTCAATTATGGGCACCAGTTCACACTGCGCCCCAc                                <  2:6767281/151‑1 (MQ=255)
                                                                                                              gggCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAATGCCCGACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCACCAGTTCACACTGCGCCCCAc                                >  2:4246160/1‑151 (MQ=255)
                                                                                                               ggCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCACCAGTTCACACTGCGCCCCACg                               <  2:2222775/151‑1 (MQ=255)
                                                                                                                  gTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCACCAGTTCACACTGCGCCCCACGGcc                            >  2:124250/1‑151 (MQ=255)
                                                                                                                   tAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCACCAGTTCACACTGCGCCCCACGGccc                           <  2:10826653/151‑1 (MQ=255)
                                                                                                                   tAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCAGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCACCAGTTCACACTGCGCCCCACGGccc                           >  2:3390785/1‑151 (MQ=255)
                                                                                                                    aGGGCGAATACCGCCACAGGCGTTATCCGC‑CGACCCTCTCCGAACCGTGCCACCCATAAGTCAGCACTTTCAAAGCCCTACGTGGTTTTCCTTCAAAAGTGCATAAAATGCTCAAGTATGGGCACCAGTTCACACTGCGCCCCACGGcccc                          >  2:7369203/1‑151 (MQ=255)
                                                                                                                    aGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCACCAGTTCACACTGCGCCCCACGGcccc                          <  2:198696/151‑1 (MQ=255)
                                                                                                                    aGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCACCAGTTCACACTGCGCCCCACGGcccc                          >  2:462070/1‑151 (MQ=255)
                                                                                                                       gCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCACCAGTTCACACTGCGCCCCACGGCCCCCAc                       >  1:7846148/1‑151 (MQ=255)
                                                                                                                       gCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCACCAGTTCACACTGCGCCCCACGGCCCCCAc                       >  1:6549349/1‑151 (MQ=255)
                                                                                                                       gCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCACCAGTTCACACTGCGCCCCACGGCCCCCAc                       >  2:10202620/1‑151 (MQ=255)
                                                                                                                         gAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCACCAGTTCACACTGCGCCCCACGGCCCCCACtt                     >  1:4664818/1‑151 (MQ=255)
                                                                                                                         gAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCACCAGTTCACACTGCGCCCCACGGCCCCCACtt                     >  1:1183095/1‑151 (MQ=255)
                                                                                                                          aaTACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCACCAGTTCACACTGCGCCCCACGGCCCCCACttt                    >  2:6417226/1‑151 (MQ=255)
                                                                                                                                 ccACAGGCGTTATCCGC‑CGCACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACACAAGAGATTAAAATGCTGATTTCTGGGCACCAGTTGACACTGCGCCCCACGGCCCCCACTTTCTCGTCt             <  1:431589/151‑1 (MQ=255)
                                                                                                                                  cacaGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCACCAGTTCACACTGCGCCCCACGGCCCCCACTTTCTCGTCtt            >  2:4518410/1‑151 (MQ=255)
                                                                                                                                  cacaGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCACCAGTTCACACTGCGCCCCACGGCCCCCACTTTCTCGTCtt            >  1:9888634/1‑151 (MQ=255)
                                                                                                                                   acaGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCACCAGTTCACACTGCGCCCCACGGCCCCCACTTTCTCGTCTTa           >  2:1647186/1‑151 (MQ=255)
                                                                                                                                   acaGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCACCAGTTCACACTGCGCCCCACGGCCCCCACTTTCTCGTCTTa           >  2:3052507/1‑151 (MQ=255)
                                                                                                                                      ggCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCACCAGTTCACACTGCGCCCCACGGCCCCCACTTTCTCGTCTTAGTg        >  2:3868875/1‑151 (MQ=255)
                                                                                                                                       gCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCACCAGTTCACACTGCGCCCCACGGCCCCCACTTTCTCGTCTTAGTGa       >  2:396886/1‑151 (MQ=255)
                                                                                                                                       gCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCACCAGTTCACACTGCGCCCCACGGCCCCCACTTTCTCGTCTTAGTGa       <  2:7699508/151‑1 (MQ=255)
                                                                                                                                        cGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCACCAGTTCACACTGCGCCCCACGGCCCCCACTTTCTCGTCTTAGTGAg      >  2:9615800/1‑151 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
AGCCGGCTGTGATGGCCGGCTTTTTTTCGCCCCAGCGAATGCCGCGCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGCGCGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCACCAGTTCACACTGCGCCCCACGGCCCCCACTTTCTCGTCTTAGTGAGCAAC  >  NC_008463/4700661‑4700952

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑