BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_008463	5,931,608	(T)_6→5	intergenic (‑27/‑151)	PA14_RS27130 ← / → PA14_RS27135	LysR family transcriptional regulator/MFS transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_008463	5,931,603	0	T	.	100.0%	2183.5 / NA	443	intergenic (‑22/‑156)	PA14_RS27130/PA14_RS27135	LysR family transcriptional regulator/MFS transporter
Reads supporting (aligned to +/- strand): ref base T (0/0); new base . (180/263); total (180/263)

Only 100 of 479 total aligned reads displayed.

CGATTTCCTCCTCCAGCGCCTGGATCTGCTGGCTCAACGGCGGCTGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTATTTAGGTGTTTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACAACGACCCCTTGTCCCTGAAATCCAGGTGGCGCGGCCTCGTTGTGCCTGCTCGCCACCGTCCCCAG  >  NC_008463/5931452‑5931749

  aTTTCCGCCTCCAGCGCCTGGATCTGCTGGCTCAACGGCGGCTGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTGttt                                                                                                                                                  <  2:8632850/151‑1 (MQ=255)

   tttCCTCCTCCAGCGCCTGGATCTGCTGGCTCAACGGCGGCTGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTGtttt                                                                                                                                                 >  1:8931202/1‑151 (MQ=255)

    ttCCTCCTCCAGCGCCTGGATCTGCTGGCTCAACGGCGGCTGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTGttttt                                                                                                                                                >  2:9508308/1‑151 (MQ=255)

      cctcctccAGCGCCTGGATCTGCTGGCTCAACGGCGGCTGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTGtttttac                                                                                                                                              <  2:7364242/151‑3 (MQ=255)

        tcctccAGCGCCTGGATCTGCTGGCTCAACGGCGGCTGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGTGTTCCAAGCGGCCCCTCCT‑CTTAGGTGtttggacat                                                                                                                                            >  2:11036192/1‑145 (MQ=255)

         cctccAGCGCCTGGATCTGCTGGCTCAACGGCGGCTGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACata                                                                                                                                          <  2:3067112/151‑1 (MQ=255)

         cctccAGCGCCTGGATCTGCTGGCTCAACGGCGGCTGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACata                                                                                                                                          >  1:8639136/1‑151 (MQ=255)

         cctccAGCGCCTGGATCTGCTGGCTCAACGGCGGCTGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACata                                                                                                                                          <  1:1655547/151‑1 (MQ=255)

          ctccAGCGCCTGGATCTGCTGGCTCAACGGCGGCTGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACatat                                                                                                                                         <  2:4675261/151‑1 (MQ=255)

          ctccAGCGCCTGGATCTGCTGGCTCAACGGCGGCTGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACatat                                                                                                                                         >  1:7853082/1‑151 (MQ=255)

             cAGCGCCTGGATCTGCTGGCTCAACGGCGGCTGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCaa                                                                                                                                      <  2:3922356/151‑1 (MQ=255)

             cAGCGCCTGGATCTGCTGGCTCAACGGCGGCTGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCaa                                                                                                                                      <  1:3319520/151‑1 (MQ=255)

             cAGCGCCTGGATCTGCTGGCTCAACGGCGGCTGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCAGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCaa                                                                                                                                      >  1:8231984/1‑151 (MQ=255)

              aGCGCCTGGATCTGCTGGCTCAACGGCGGCTGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAAt                                                                                                                                     >  2:9690901/1‑151 (MQ=255)

                    tGGATCTGCTGGCTCAACGGCGGCTGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTc                                                                                                                               >  1:9296947/1‑151 (MQ=255)

                     ggATCTGCTGGCTCAACGGCGGCTGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCg                                                                                                                              <  1:5919159/151‑1 (MQ=255)

                      gATCTGCTGGCTCAACGGCGGCTGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGa                                                                                                                             <  1:5742536/151‑1 (MQ=255)

                       aTCTGCTGGCTCAACGGCGGCTGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGAt                                                                                                                            <  2:3464210/151‑1 (MQ=255)

                        tCTGCTGGCTCAACGGCGGCTGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATc                                                                                                                           <  2:9195890/151‑1 (MQ=255)

                         ctgctgGCTCAACGGCGGCTGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCa                                                                                                                          <  2:10656722/151‑1 (MQ=255)

                             tgGCTCAACTGCGGCTGGGAGATCCCCGGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCACCTCCTA‑TTAGGTG‑TTTTTACATTTCAAGCGTGTCGATCAatat                                                                                                                      >  2:6200875/1‑151 (MQ=255)

                             tgGCTCAACGGCGGCTGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAatat                                                                                                                      >  2:7968373/1‑151 (MQ=255)

                             tgGCTCAACGGCGGCTGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAatat                                                                                                                      <  1:7327448/151‑1 (MQ=255)

                             tgGCTCAACGGCGGCTGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAatat                                                                                                                      <  2:6753070/151‑1 (MQ=255)

                              ggCTCAACGGCGGCTGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAatcta                                                                                                                     <  1:7527444/151‑1 (MQ=255)

                                  cAACGGCGGCTGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTgg                                                                                                                 >  1:517215/1‑151 (MQ=255)

                                        cggcTGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCa                                                                                                           <  1:4550010/151‑1 (MQ=255)

                                          gcTGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCata                                                                                                         <  1:6443784/151‑1 (MQ=255)

                                            tGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCAtata                                                                                                       <  1:6140724/151‑1 (MQ=255)

                                             gggAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATaa                                                                                                      >  1:1929544/1‑151 (MQ=255)

                                               gagaTCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAAc                                                                                                    >  1:6683868/1‑151 (MQ=255)

                                                agaTCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAAcc                                                                                                   >  1:1173936/1‑151 (MQ=255)

                                                    ccccAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCT‑TTTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACctatc                                                                                               <  1:10849484/151‑1 (MQ=255)

                                                       cAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTGTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACctatctat                                                                                            <  2:10811869/151‑1 (MQ=255)

                                                        aGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATCGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCtata                                                                                           >  1:4219208/1‑151 (MQ=255)

                                                        aGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCtata                                                                                           >  2:5918128/1‑151 (MQ=255)

                                                        aGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCtata                                                                                           <  2:6439413/151‑1 (MQ=255)

                                                        aGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCtata                                                                                           <  1:3273785/151‑1 (MQ=255)

                                                         ggCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTatat                                                                                          <  1:3582543/151‑1 (MQ=255)

                                                         ggCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTatat                                                                                          <  2:7666284/151‑1 (MQ=255)

                                                           cgcTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATcc                                                                                        >  1:4919141/1‑151 (MQ=255)

                                                           cgcTCGGCGGCGCCGCCGACGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCAGAGTTCCACGCGGCCCCTCCT‑CTAAGCTG‑TTTTTACATATCAATCGTGACGATCAATCTATTGGAAATCATATAAACCTATCTAGATcc                                                                                        <  2:11129907/151‑1 (MQ=255)

                                                            gcTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCt                                                                                       <  2:9614766/151‑1 (MQ=255)

                                                            gcTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCt                                                                                       <  1:2286931/151‑1 (MQ=255)

                                                              tCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCttt                                                                                     >  2:5617471/1‑151 (MQ=255)

                                                               cggcggCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTg                                                                                    >  1:7075603/1‑151 (MQ=255)

                                                               cggcggCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTg                                                                                    <  2:7473759/151‑1 (MQ=255)

                                                                ggcggcGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGc                                                                                   >  2:544697/1‑151 (MQ=255)

                                                                         gcCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAAcc                                                                          <  1:9618791/151‑1 (MQ=255)

                                                                         gcCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAAcc                                                                          <  1:3768412/151‑1 (MQ=255)

                                                                           cGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTc                                                                        <  1:290194/151‑1 (MQ=255)

                                                                            gAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTcc                                                                       <  1:9758010/151‑1 (MQ=255)

                                                                              cgTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCGTTGCCACATAACCTCcag                                                                     >  2:6903210/2‑150 (MQ=255)

                                                                                tGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCcaaca                                                                   <  1:8852076/151‑1 (MQ=255)

                                                                                 gCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCcaacaa                                                                  <  1:1238150/151‑1 (MQ=255)

                                                                                  cAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCaacaac                                                                 <  1:2724031/151‑1 (MQ=255)

                                                                                    gCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACAACGa                                                               <  1:10001637/151‑1 (MQ=255)

                                                                                     ctcctcGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACAACGAc                                                              <  1:9186575/151‑1 (MQ=255)

                                                                                     ctcctcGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACAACGAc                                                              >  1:2327562/1‑151 (MQ=255)

                                                                                           ggcgacggcgaTGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACAACGACCCCtgg                                                        >  1:8494262/1‑149 (MQ=255)

                                                                                           ggcgacggcgaTGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACAACGACCCCTTg                                                        <  1:11199725/151‑1 (MQ=255)

                                                                                            gcgacggcgaTGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACAACGACCCCTTGt                                                       >  2:2556896/1‑151 (MQ=255)

                                                                                                    gaTGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACAACGACCCCTTGTCCCTGaaa                                               <  2:4584652/151‑1 (MQ=255)

                                                                                                    gaTGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACAACGACCCCTTGTCCCTGaaa                                               <  1:10100157/151‑1 (MQ=255)

                                                                                                    gaTGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACAACGACCCCTTGACCCTGaaa                                               <  2:3842553/151‑1 (MQ=255)

                                                                                                     aTGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACAACGACCCCTTGTCCCTGAAAt                                              >  2:2050633/1‑151 (MQ=255)

                                                                                                         aagaGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACAACGACCCCTTGTCCCTGAAATCCAg                                          <  2:8150601/148‑1 (MQ=255)

                                                                                                         aaTAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACAACGACCCCTTGTCCCTGAAATCCAg                                          <  2:1394911/151‑1 (MQ=255)

                                                                                                          aTAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACAACGACCCCTTGTCCCTGAAATCCAgg                                         >  2:2315038/1‑151 (MQ=255)

                                                                                                           tAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACAACGACCCCTTGTCCCTGAAATCCAGGt                                        <  2:9410449/151‑1 (MQ=255)

                                                                                                              cgcAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACAACGACCCCTTGTCCCTGAAATCCAGGTGgc                                     >  2:4259638/1‑151 (MQ=255)

                                                                                                              cgcAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACAACGACCCCTTGTCCCTGAAATCCAGGTGgc                                     >  1:2445461/1‑151 (MQ=255)

                                                                                                               ccAGGTGCCTGAGTTCCACGCGGCCCCTCCTA‑GTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATCCACCTATCAATATCCTTTGCCACAATAACTCCAACAACGACCCCTTCACCCTGAAATCCaggaggag                                    >  2:8866727/2‑149 (MQ=255)

                                                                                                                cAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAAAATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACAACGACCCCTTGTCCCTGAAATCCAGGTGgcgc                                   >  1:1539635/1‑151 (MQ=255)

                                                                                                                  ggTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACCACGACCCCTTGTCCCTGAAATCCAGGTGGCGCgg                                 <  2:9834835/151‑1 (MQ=255)

                                                                                                                  ggTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACAACGACCCCTTGTCCCTGAAATCCAGGTGGCGCgg                                 <  1:7177524/151‑1 (MQ=255)

                                                                                                                    tGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACAACGACCCCTTGTCCCTGAAATCCAGGTGGCGCGGcc                               >  1:3826568/1‑151 (MQ=255)

                                                                                                                     gCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACAACGACCCCTTGTCCCTGAAATCCAGGTGGCGCGGCCt                              >  1:1780313/1‑151 (MQ=255)

                                                                                                                      ccTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACAACGACCCCTTGTCCCTGAAATCCAGGTGGCGCGGCCTc                             <  2:7857834/151‑1 (MQ=255)

                                                                                                                      ccTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACAACGACCCCTTGTCCCTGAAATCCAGGTGGCGCGGCCTc                             >  1:298456/1‑151 (MQ=255)

                                                                                                                      ccTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACAACGACCCCTTGTCCCTGAAATCCAGGTGGCGCGGCCTc                             <  1:820250/151‑1 (MQ=255)

                                                                                                                       cTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACAACGACCCCTTGTCCCTGAAATCCAGGTGGCGCGGCCTCg                            >  2:4421379/1‑151 (MQ=255)

                                                                                                                         gAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGACATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACAACGACCCCTTGTCCCTGAAATCCAGGTGGCGCGGCCTCGtt                          <  2:4921725/151‑1 (MQ=255)

                                                                                                                          aGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACAACGACCCCTTGTCCCTGAAATCCAGGTGGCGCGGCCTCGTtg                         >  2:175435/1‑151 (MQ=255)

                                                                                                                          aGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACAACGACCCCTTGTCCCTGAAATCCAGGTGGCGCGGCCACGTtg                         >  2:1513396/1‑151 (MQ=255)

                                                                                                                           gTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACAACGACCCCTTGTCCCTGAAATCCAGGTGGCGCGGCCTCGTtgt                        >  2:1001716/1‑151 (MQ=255)

                                                                                                                            ttCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACAACGACCCCTTGTCCCTGAAATCCAGGTGGCGCGGCCTCGTtgtg                       <  2:846505/151‑1 (MQ=255)

                                                                                                                             tCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACAACGACCCCGTGTCCCTGAACTCCAGGTGGCGCGGCCGCGTTGTGc                      >  2:5704481/1‑151 (MQ=255)

                                                                                                                              ccAAGCGGCCCCTCCTA‑TTAGGTG‑ATTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACAACGACCCCTTGTCCCTGAAATCCAGGTGGCGCGGCCTCGTTGTGcc                     >  1:1072181/1‑151 (MQ=255)

                                                                                                                               cAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACAACGACCCCTTGTCCCTGAAATCCAGGTGGCGCGGCCTCGTTGTGCCt                    >  1:11013453/1‑151 (MQ=255)

                                                                                                                               cAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACAACGACCCCTTGTCCCTGAAATCCAGGTGGCGCGGCCTCGTTGTGCCt                    >  1:542610/1‑151 (MQ=255)

                                                                                                                               cAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACAACGACCCCTTGTCCCTGAAATCCAGGTGGCGCGGCCTCGTTGTGCCt                    >  2:1930902/1‑151 (MQ=255)

                                                                                                                                    ggCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACAACGACCACTTGTCCCTGAAATCCAGGTGGCGCGGCCTCGTTGTGCCTGCTCg               >  2:3622815/1‑151 (MQ=255)

                                                                                                                                     gCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACAACGACCCCTTGTCCCTGAAATCCAGGTGGCGCGGCCTCGTTGTGCCTGCTCGc              >  2:2791514/1‑151 (MQ=255)

                                                                                                                                        cctcctA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACAACGACCCCTTGTCCCTGAAATCCAGGTGGCGCGGCCTCGTTGTGCCTGCTCGCCAc           >  2:5216787/1‑151 (MQ=255)

                                                                                                                                          tcctA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACAACGACCCCTTGTCCCTGAAATCCAGGTGGCGCGGCCTCGTTGTGCCTGCTCGCCACCg         >  1:3989795/1‑151 (MQ=255)

                                                                                                                                              tattAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACAACGACCCCTTGTCCCTGAAATCCAGGTGGCGCGGCCTCGTTGTGCCTGCTCGCCACCGTcc      >  2:8993758/3‑151 (MQ=255)

                                                                                                                                              tattAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACAACGACCCCTTGTCCCTGAAATCCAGGTGGCGCGGCCTCGTTGTGCCTGCTCGCCACCGTcc      >  2:6809231/3‑151 (MQ=255)

                                                                                                                                                ttAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACAACGACCCCTTGTCCCTGAAATCCAGGTGGCGCGGCCTCGTTGTGCCTGCTCGCCACCGTcccc    >  1:1382250/1‑151 (MQ=255)

                                                                                                                                                  aGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCGTTGCCACATAACCTCCAACAACGACCCCTTGTCCCTGAAATCCAGGTGGCGCGGCCTCGTTGTGCCTGCTCGCCACCGTCCCCag  >  1:2097141/1‑151 (MQ=255)

CGATTTCCTCCTCCAGCGCCTGGATCTGCTGGCTCAACGGCGGCTGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTATTTAGGTGTTTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACAACGACCCCTTGTCCCTGAAATCCAGGTGGCGCGGCCTCGTTGTGCCTGCTCGCCACCGTCCCCAG  >  NC_008463/5931452‑5931749

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`