Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 3,670,543 +A coding (543/1020 nt) PA14_RS16675 → ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084633,670,5421.A99.2% 1676.4 / 1.8 402coding (542/1020 nt)PA14_RS16675ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base . (2/1);  new base A (183/216);  total (185/217)
Fisher's exact test for biased strand distribution p-value = 5.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 422 total aligned reads displayed.

GACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCGTTTTCCTGG‑AGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTCCGCGCCGAGTTCCTCCGCGGG  >  NC_008463/3670391‑3670687
                                                                                                                                                        |                                                                                                                                                 
gACGTGCTCGCCCGGGTGATTTACGGTTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑GTTCCTgg                                                                                                                                                    >  1:10446074/1‑151 (MQ=255)
gACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTgg                                                                                                                                                    >  1:2793997/1‑151 (MQ=255)
 aCGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGG‑a                                                                                                                                                  >  1:11028597/1‑151 (MQ=255)
 aCGTGCTCGACCGGGTGATTTACTGCTTCCGCGTGTCGATCCTCTTCGCCCTGAACCTGACCGAGCTCAGTTATCTGATCTGCGTCACCGTCGGCGCCCTGCAAGGATAATACTGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGG‑a                                                                                                                                                  >  2:6141291/1‑151 (MQ=255)
  cGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGG‑aa                                                                                                                                                 <  2:3492535/151‑2 (MQ=255)
   gTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCAACTACGGCGGCACGGTCGACCTGCTCGGCCAGCG‑TTTCCTGG‑aag                                                                                                                                                >  2:3295847/1‑149 (MQ=255)
    tGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGG‑aagt                                                                                                                                               >  1:10505521/1‑148 (MQ=255)
      cTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGG‑aagtct                                                                                                                                             >  2:4949351/1‑146 (MQ=255)
           ccGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCg                                                                                                                                         <  1:3315716/151‑1 (MQ=255)
           ccGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCg                                                                                                                                         >  2:5998675/1‑151 (MQ=255)
             gggTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCggg                                                                                                                                       >  1:1469042/1‑151 (MQ=255)
              ggTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCgggg                                                                                                                                      <  2:3945386/151‑1 (MQ=255)
              ggTGATTTACCGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCgggg                                                                                                                                      >  2:8363566/1‑151 (MQ=255)
               gTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCCAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGc                                                                                                                                     >  2:1536574/1‑151 (MQ=255)
                tGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCt                                                                                                                                    <  1:2574645/151‑1 (MQ=255)
                 gATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTg                                                                                                                                   <  1:7676694/151‑1 (MQ=255)
                         gCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGct                                                                                                                           >  2:1937323/1‑151 (MQ=255)
                             ccGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCACGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTAc                                                                                                                       >  2:119491/1‑151 (MQ=255)
                               gcgTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACct                                                                                                                     <  1:10015023/151‑1 (MQ=255)
                                cgTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACctg                                                                                                                    <  1:1552546/151‑1 (MQ=255)
                                 gtgtCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACctgc                                                                                                                   >  1:314961/1‑151 (MQ=255)
                                 gtgtCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACctgc                                                                                                                   >  2:1718125/1‑151 (MQ=255)
                                  tgtCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACctgct                                                                                                                  >  2:3217898/1‑151 (MQ=255)
                                     cGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGat                                                                                                               <  2:844588/151‑1 (MQ=255)
                                      gctCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGatc                                                                                                              <  1:5296460/149‑1 (MQ=255)
                                      gATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGatc                                                                                                              <  1:10212750/151‑1 (MQ=255)
                                      gATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGatc                                                                                                              <  1:4235175/151‑1 (MQ=255)
                                       aTCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGatca                                                                                                             >  1:8734144/1‑151 (MQ=255)
                                          ctctTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATcc                                                                                                          >  1:7402112/1‑151 (MQ=255)
                                          ctctTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATcc                                                                                                          >  1:6231625/1‑151 (MQ=255)
                                             ttCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTgg                                                                                                       <  2:9698148/151‑1 (MQ=255)
                                              tCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCAGCCTGGc                                                                                                      >  2:10507250/1‑151 (MQ=255)
                                                 ccctgaccctgaCCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAg                                                                                                   >  2:3397599/1‑151 (MQ=255)
                                                 ccctgaccctgaCCGTGCTCAGTTCACTGATCGGCGTCTCCGTCGGAGCCCTGCAAGGATACTACGTCGTCTGGGTCGACCTGATCTGCCAGAG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCTGTGCTCTACCTGCTGATCATCCTGGCCAg                                                                                                   <  2:6737372/151‑1 (MQ=255)
                                                    tgaccctgaccGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCtt                                                                                                <  1:7070948/151‑1 (MQ=255)
                                                    tgaccctgaccGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCtt                                                                                                <  2:3682810/151‑1 (MQ=255)
                                                     gaccctgaccGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTc                                                                                               <  1:5967070/151‑1 (MQ=255)
                                                     gaccctgaccGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTc                                                                                               >  1:5549299/1‑151 (MQ=255)
                                                       ccctgaccGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGt                                                                                             <  1:8518497/151‑1 (MQ=255)
                                                         ctgaccGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCGCGGCAAGC‑TTTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGTTCATCCTGGCCAGCTTCGTcc                                                                                           >  2:2657224/1‑151 (MQ=255)
                                                          tgaccGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCa                                                                                          >  2:4314840/1‑151 (MQ=255)
                                                          tgaccGTGCTCAGTTCTCTGACCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCa                                                                                          >  1:4705604/1‑151 (MQ=255)
                                                           gaccGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAg                                                                                         >  1:637947/1‑151 (MQ=255)
                                                            accGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGc                                                                                        >  2:2959295/1‑151 (MQ=255)
                                                             ccGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGcc                                                                                       >  1:3441332/1‑151 (MQ=255)
                                                             ccGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGcc                                                                                       >  1:4908161/1‑151 (MQ=255)
                                                                  cTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACt                                                                                  >  1:3679959/1‑151 (MQ=255)
                                                                     aGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCGTCGTCCAGCCGAACTTCt                                                                               >  2:3049493/1‑151 (MQ=255)
                                                                     aGTTCTCTGATCGGCGTCACCGTCGGCGCCCAGCAAGGCTACTACGGCGGCTGGGTCGACCCGCTCGGCCAGCGTGTCCTCGA‑AGTCTGGTCGGGGCTGCCGGCGCACGACCTGCTGATCATCCTGGCCACCATCGTCCAGCCGAACTTCt                                                                               <  2:509368/151‑1 (MQ=255)
                                                                      gTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCtg                                                                              <  1:5668156/151‑1 (MQ=255)
                                                                            tGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGgct                                                                        <  2:7299190/151‑1 (MQ=255)
                                                                              aTCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGgctgc                                                                      >  2:8640886/1‑151 (MQ=255)
                                                                                cGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGctgctg                                                                    >  2:6121702/1‑151 (MQ=255)
                                                                                cGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGctgctg                                                                    <  1:3582442/151‑1 (MQ=255)
                                                                                cGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGatgatg                                                                    >  2:1013281/1‑151 (MQ=255)
                                                                                 ggCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTgg                                                                   >  1:9628297/1‑151 (MQ=255)
                                                                                  gCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTggg                                                                  <  2:8226639/151‑1 (MQ=255)
                                                                                  gCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTggg                                                                  <  1:2909445/151‑1 (MQ=255)
                                                                                     tCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGcat                                                               <  2:2781112/151‑1 (MQ=255)
                                                                                     tCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGcat                                                               <  2:2186377/151‑1 (MQ=255)
                                                                                       aCCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGcatca                                                             >  1:8027178/1‑151 (MQ=255)
                                                                                        ccGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGcatcat                                                            <  2:10988070/151‑1 (MQ=255)
                                                                                           tCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCAtgct                                                         >  1:5835002/1‑151 (MQ=255)
                                                                                            cGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCAtgctg                                                        <  2:1270663/151‑1 (MQ=255)
                                                                                            cGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCAtgctg                                                        >  2:958730/1‑151 (MQ=255)
                                                                                             ggCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCAtgctgc                                                       >  1:1085809/1‑151 (MQ=255)
                                                                                                  ccTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGttt                                                  <  2:9061984/151‑1 (MQ=255)
                                                                                                  ccTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGttt                                                  <  1:1987065/151‑1 (MQ=255)
                                                                                                    tGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTc                                                <  2:7959552/151‑1 (MQ=255)
                                                                                                    tGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTc                                                <  2:6476947/151‑1 (MQ=255)
                                                                                                        aGGCTACTTCGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTgg                                            <  1:10627763/151‑1 (MQ=255)
                                                                                                        aGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTgg                                            >  2:9501703/1‑151 (MQ=255)
                                                                                                             actacGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAg                                       >  1:6926051/1‑151 (MQ=255)
                                                                                                             actacGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAg                                       >  1:11141528/1‑151 (MQ=255)
                                                                                                               tacGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGcc                                     >  1:5010192/1‑151 (MQ=255)
                                                                                                                 cggcggCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCtg                                   <  1:2319432/151‑1 (MQ=255)
                                                                                                                  ggcggcTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCtgg                                  >  1:8264724/1‑151 (MQ=255)
                                                                                                                   gcggcTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCtggt                                 <  1:9305431/151‑1 (MQ=255)
                                                                                                                   gcggcTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCtggt                                 <  1:1283030/151‑1 (MQ=255)
                                                                                                                         gggTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGt                           <  2:576074/151‑1 (MQ=255)
                                                                                                                             cGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTc                       >  1:6082863/1‑151 (MQ=255)
                                                                                                                              gACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTcc                      >  2:5273272/1‑151 (MQ=255)
                                                                                                                              gACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTcc                      >  1:9926205/1‑151 (MQ=255)
                                                                                                                               aCCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTCcg                     >  1:6542377/1‑151 (MQ=255)
                                                                                                                                ccTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTCcgc                    >  2:4081480/1‑151 (MQ=255)
                                                                                                                                 cTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTCcgcg                   >  1:8921358/1‑151 (MQ=255)
                                                                                                                                  tGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTCCgcgc                  >  2:4792456/1‑151 (MQ=255)
                                                                                                                                   gCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCCGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTCCGCGcc                 <  1:6008441/151‑1 (MQ=255)
                                                                                                                                     tCGGCCAGCG‑TGTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTCCGCGCCGa               <  1:10795557/151‑1 (MQ=255)
                                                                                                                                       ggCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTCCGCGCCGAGt             >  1:9038084/1‑151 (MQ=255)
                                                                                                                                       ggCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTCCGCGCCGAGt             <  1:9439948/151‑1 (MQ=255)
                                                                                                                                       ggCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTCCGCGCCGAGt             <  2:10717903/151‑1 (MQ=255)
                                                                                                                                        gCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTCCGCGCCGAGtt            >  1:3928702/1‑151 (MQ=255)
                                                                                                                                             gcgtttCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGACCTTCTGGTGGCTGCTGGGCCTCTGGCTGCTGTGTGCCTGGAGGAGCCTGGTGTACGTGGTCCGCGCCGAGTtcctc        >  2:4219851/4‑151 (MQ=255)
                                                                                                                                              cgtttCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTCCGCGCCGAGTtcctcc       >  1:7813150/3‑151 (MQ=255)
                                                                                                                                               gtttCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTCCGCGCCGAGTTCCTCcg      <  1:2730104/150‑1 (MQ=255)
                                                                                                                                                tttCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCGGTTTTCCTGGATGAGCCTGGTGGACGTGGTCCGCGCCGAGTTCCTCcgc     >  2:6356943/1‑151 (MQ=255)
                                                                                                                                                 ttCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTCCGCGCCGAGTTCCTCcgcg    >  2:6959691/1‑151 (MQ=255)
                                                                                                                                                 ttCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTCCGCGCCGAGTTCCTCcgcg    >  2:4411189/1‑151 (MQ=255)
                                                                                                                                                  tCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTCCGCGCCGAGTTCCTCCGCgg   <  1:11001173/151‑1 (MQ=255)
                                                                                                                                                        |                                                                                                                                                 
GACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCGTTTTCCTGG‑AGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTCCGCGCCGAGTTCCTCCGCGGG  >  NC_008463/3670391‑3670687

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑