Predicted mutation
evidence seq id position mutation annotation gene description
MC JC NC_008463 5,131,729 Δ34 bp pseudogene (288‑321/1180 nt) PA14_RS23490 ← acyl‑CoA dehydrogenase family protein

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ NC_008463 5131729 5131762 34 638 [0] [1] 637 PA14_RS23490 acyl‑CoA dehydrogenase family protein

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_008463 = 51317280 (0.000)545 (1.210) 211/264 0.0 100% pseudogene (322/1180 nt) PA14_RS23490 acyl‑CoA dehydrogenase family protein
?NC_008463 5131763 = 0 (0.000)pseudogene (287/1180 nt) PA14_RS23490 acyl‑CoA dehydrogenase family protein

Only 100 of 638 total aligned reads displayed.

TACCGCCGTGGTCTTCACCCCTTGCAGGTCGGAACCGGCCCCCGGCTCGGTCATGGCGATGGCCGCGACCATCTCGCCGGATACCAGCTTCGGCAGGTACTTGCGCTTCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGGCGCAGCGATGTCCGAATGTAGGGCGCAGCGATGTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCCCGACCGATCTCTTCGATGACCACGGCGCTGTAGAGGAAGTCCGCGCCCATCCCGCCATACTCTTCCGGCAGGT  >  NC_008463/5131578‑5131874
                                                                                                                                                      |                                                                                                                                                  
  ccgccgTGGTCTTCACCCCTTGCAGGTCGGAACCGGCCCCCGGCTCGGTCATGGCGATGGCCGCGACCATCTCGCCGGATACCAGCTTCGGCAGGTACTTGCGCTTCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtc                                                                                                                                                  <  2:4797097‑M1/151‑3 (MQ=255)
   cgccgTGGTCTTCACCCCTTGCAGGTCGGAACCGGCCCCCGGCTCGGTCATGGCGATGGCCGCGACCATCTCGCCGGATACCAGCTTCGGCAGGTACTTGCGCTTCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtcc                                                                                                                                                 <  1:10837071‑M1/151‑4 (MQ=255)
    gccgTGGTCTTCACCCCTTGCAGGTCGGAACCGGCCCCCGGCTCGGTCATGGCGATGGCCGCGACCATCTCGCCGGATACCAGCTTCGGCAGGTACTTGCGCTTCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccg                                                                                                                                                >  1:10004909‑M1/1‑147 (MQ=255)
       gTGGTCTTCACCCCTTGCAGGTCGGAACCGGCCCCCGGCTCGGTCATGGCGATGGCCGCGACCATCTCGCCGGATACCAGCTTCGGCAGGTACTTGCGCTTCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccgaat                                                                                                                                             >  2:9502604‑M1/1‑144 (MQ=255)
       gTGGTCTTCACCCCTTGCAGGTCGGAACCGGCCCCCGGCTCGGTCATGGCGATGGCCGCGACCATCTCGCCGGATACCAGCTTCGGCAGGTACTTGCGCTTCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccgaat                                                                                                                                             >  2:2665729‑M1/1‑144 (MQ=255)
         ggTCTTCACCCCTTGCAGGTCGGAACCGGCCCCCGGCTCGGTCATGGCGATGGCCGCGACCATCTCGCCGGATACCAGCTTCGGCAGGTACTTGCGCTTCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccgaatgc                                                                                                                                           >  1:5414573‑M1/1‑142 (MQ=255)
            cTTCACCCCTTGCAGGTCGGAACCGGCCCCCGGCTCGGTCATGGCGATGGCCGCGACCATCTCGCCGGATACCAGCTTCGGCAGGTACTTGCGCTTCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccgaatgcagg                                                                                                                                        <  1:2406568‑M1/151‑13 (MQ=255)
              tCACCCCTTGCAGGTCGGAACCGGCCCCCGGCTCGGTCATGGCGATGGCCGCGACCATCTCGCCGGATACCAGCTTCGGCAGGTACTTGCGATTCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccgaatgcaggga                                                                                                                                      <  1:2146237‑M1/151‑15 (MQ=255)
                aCCCCTTGCAGGTCGGAACCGGCCCCCGGCTCGGTCATGGCGATGGCCGCGACCATCTCGCCGGATACCAGCTTCGGCAGGTACTTGCGCTTCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccgaatgcagggaga                                                                                                                                    >  1:7111435‑M1/1‑135 (MQ=255)
                 ccccTTGCAGGTCGGAACCGGCCCCCGGCTCGGTCATGGCGATGGCCGCGACCATCTCGCCGGATACCAGCTTCGGCAGGTACTTGCGCTTCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccgaatgcagggagaa                                                                                                                                   <  1:10546554‑M1/151‑18 (MQ=255)
                     ttGCAGGTCGGAACCGGCCCCCGGCTCGGTCATGGCGATGGCCGCGACCATCTCGCCGGATACCAGCTTCGGCAGGTACTTGCGCTTCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccgaatgcagggagaaaccg                                                                                                                               <  2:10875609‑M1/151‑22 (MQ=255)
                      tGCAGGTCGGAACCGGCCCCCGGCTCGGTCATGGCGATGGCCGCGACCATCTCGCCGGATACCAGCTTCGGCAGGTACTTGCGCTTCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccgaatgcagggagaaaccga                                                                                                                              >  1:5593826‑M1/1‑129 (MQ=255)
                        cAGGTCGGAACCGGCCCCCGGCTCGGTCATGGCGATGGCCGCGACCATCTCGCCGGATACCAGCTTCGGCAGGTACTTGCGCTTCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatc                                                                                                                            <  1:5686452‑M1/151‑25 (MQ=255)
                         aGGTCGGAACCGGCCCCCGGCTCGGTCATGGCGATGGCCGCGACCATCTCGCCGGATACCAGCTTCGGCAGGTACTTGCGCTTCAGCGCCTCGCTGCCGTAGTGGAGGAGGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcc                                                                                                                           >  2:167931‑M1/1‑126 (MQ=255)
                           gTCGGAACCGGCCCCCGGCTCGGTCATGGCGATGGCCGCGACCATCTCGCCGGATACCAGCTTCGGCAGGTACTTGCGCTTCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccg                                                                                                                         <  1:4115982‑M1/151‑28 (MQ=255)
                              ggAACCGGCCCCCGGCTCGGTCATGGCGATGGCCGCGACCATCTCGCCGGATACCAGCTTCGGCAGGTACTTGCGCTTCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccgaatgcagggagaacccgatcccggtc                                                                                                                      >  2:1018231‑M1/1‑121 (MQ=255)
                              ggAACCGGCCCCCGGCTCGGTCATGGCGATGGCCGCGACCATCTCGCCGGATACCAGCTTCGGCAGGTACTTGCGCTTCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccggtc                                                                                                                      <  1:695540‑M1/151‑31 (MQ=255)
                               gAACCGGCCCCCGGCTCGGTCATGGCGATGGCCGCGACCATCTCGCCGGATACCAGCTTCGGCAGGTACTTGCGCTTCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccggtca                                                                                                                     >  1:3247303‑M1/1‑120 (MQ=255)
                                  ccGGCCCCCGGCTCGGTCATGGCGATGGCCGCGACCATCTCGCCGGATACCAGCTTCGGCAGGTACTTGCGCTTCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccggtcaggc                                                                                                                  <  1:2583252‑M1/151‑35 (MQ=255)
                                    ggCCCCCGGCTCGGTCATGGCGATGGCCGCGACCATCTCGCCGGATACCAGCTTCGGCAGGTACTTGCGCTTCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccggtcaggccg                                                                                                                >  1:2873486‑M1/1‑115 (MQ=255)
                                    ggCCCCCGGCTCGGTCATGGCGATGGCCGCGACCATCTCGCCGGATACCAGCTTCGGCAGGTACTTGCGCTTCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccggtcaggccg                                                                                                                <  1:4799725‑M1/151‑37 (MQ=255)
                                      cccccGGCTCGGTCATGGCGATGGCCGCGACCATCTCGCCGGATACCAGCTTCGGCAGGTACTTGCGCTTCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccggtcaggccggc                                                                                                              <  2:8270821‑M1/151‑39 (MQ=255)
                                          cGGCTCGGTCATGGCGATGGCCGCGACCATCTCGCCGGATACCAGCTTCGGCAGGAACTTGCGCTTCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccggtcaggccggcccga                                                                                                          <  2:7433128‑M1/151‑43 (MQ=255)
                                           ggCTCGGTCATGGCGATGGCCGCGACCATCTCGCCGGATACCAGCTTCGGCAGGTACTTGCGCTTCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccggtcaggccggcccgac                                                                                                         >  2:7715867‑M1/1‑108 (MQ=255)
                                            gCTCGGTCATGGCGATGGCCGCGACCATCTCGCCGGATACCAGCTTCGGCAGGTACTTGCGCTTCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccggtcaggccggcccgacc                                                                                                        >  1:5132002‑M1/1‑107 (MQ=255)
                                             cTCGGTCATGGCGATGGCCGCGACCATCTCGCCGGATACCAGCTTCGGCAGGTACTTGCGCTTCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccggtcaggccggcccgaccg                                                                                                       <  2:5929099‑M1/151‑46 (MQ=255)
                                               cGGTCATGGCGATGGCCGCGACCATCTCGCCGGATACCAGCTTCGGCAGGTACTTGCGCTTCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccggtcaggccggcccgaccgat                                                                                                     >  1:7293742‑M1/1‑104 (MQ=255)
                                                 gTCATGGCGATGGCCGCGACCATCTCGCCGGATACCAGCTTCGGCAGGTACTTGCGCTTCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccggtcaggccggcccgaccgatct                                                                                                   <  1:9277680‑M1/151‑50 (MQ=255)
                                                   cATGGCGATGGCCGCGACCATCTCGCCGGATACCAGCTTCGGCAGGTACTTGCGCTTCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccggtcaggccggcccgaccgatctct                                                                                                 >  1:9335778‑M1/1‑100 (MQ=255)
                                                   cATGGCGATGGCCGCGACCATCTCGCCGGATACCAGCTTCGGCAGGTACTTGCGCTTCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccggtcaggccggcccgaccgatctct                                                                                                 <  1:3921743‑M1/151‑52 (MQ=255)
                                                    aTGGCGATGGCCGCGACCATCTCGCCGGATACCAGCTTCGGCAGGTACTTGCGCTTCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccggtcaggccggcccgaccgatctctt                                                                                                >  2:3192432‑M1/1‑99 (MQ=255)
                                                          aTGGCCGCGACCATCTCGCCGGATACCAGCTTCGGCAGGTACTTGCGCTTCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccggtcaggccggcccgaccgatctcttcgatga                                                                                          >  2:7948726‑M1/1‑93 (MQ=255)
                                                            ggCCGCGACCATCTCGCCGGATACCAGCTTCGGCAGGTACTTGCGCTTCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccggtcaggccggcccgaccgatctcttcgatgacc                                                                                        >  2:10535926‑M1/1‑91 (MQ=255)
                                                               cgcgACCATCTCGCCGGATACCAGCTTCGGCAGGTACTTGCGCTTCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccggtcaggccggcccgaccgatctcttcgatgaccacg                                                                                     <  1:8505325‑M1/151‑64 (MQ=255)
                                                                 cgACCATCTCGCCGGATACCAGCTTCGGCAGGTACTTGCGCTTCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccggtcaggccggcccgaccgatctcttcgatgaccacggc                                                                                   <  2:386237‑M1/151‑66 (MQ=255)
                                                                  gACCATCTCGCCGGATACCAGCTTCGGCAGGTACTTGCGCTTCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccggtcaggccggcccgaccgatctcttcgatgaccacggcg                                                                                  >  2:3382624‑M1/1‑85 (MQ=255)
                                                                     cATCTCGCCGGATACCAGCTTCGGCAGGTACTTGCGCTTCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccggtcaggccggcccgaccgatctcttcgatgaccacggcgctg                                                                               <  2:9261772‑M1/151‑70 (MQ=255)
                                                                      aTCTCGCCGGATACCAGCTTCGGCAGGTACTTGCGCTTCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccggtcaggccggcccgaccgatctcttcgatgaccacggcgctgt                                                                              >  1:2960477‑M1/1‑81 (MQ=255)
                                                                          cGCCGGATACCAGCTTCGGCAGGTACTTGCGCTTCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccggtcaggccggcccgaccgatctcttcgatgaccacggcgctgtagag                                                                          <  2:5591908‑M1/151‑75 (MQ=255)
                                                                               gATACCAGCTTCGGCAGGTACTTGCGCTTCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccggtcaggccggcccgaccgatctcttcgatgaccacggcgctgtagaggaagt                                                                     <  2:5292459‑M1/151‑80 (MQ=255)
                                                                                aTACCAGCTTCGGCAGGTACTTGCGCTTCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccggtcaggccggcccgaccgatctcttcgatgaccacggcgctgtagaggaagtc                                                                    <  1:10362254‑M1/151‑81 (MQ=255)
                                                                                  aCCAGCTTCGGCAGGTACTTGCGCTTCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccggtcaggccggcccgaccgatctcttcgatgaccacggcgctgtagaggaagtccg                                                                  <  1:2734189‑M1/151‑83 (MQ=255)
                                                                                   ccAGCTTCGGCAGGTACTTGCGCTTCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccggtcaggccggcccgaccgatctcttcgatgaccacggcgctgtagaggaagtccgc                                                                 >  1:5908339‑M1/1‑68 (MQ=255)
                                                                                     aGCTTCGGCAGGTACTTGCGCTTCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccggtcaggccggcccgaccgatctcttcgatgaccacggcgctgtagaggaagtccgcgc                                                               >  1:8890981‑M1/1‑66 (MQ=255)
                                                                                      gCTTCGGCAGGTACTTGCGCTTCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccggtcaggccggcccgaccgatctcttcgatgaccacggcgctgtagaggaagtccgcgcc                                                              >  2:8591762‑M1/1‑65 (MQ=255)
                                                                                       cTTCGGCAGGTACTTGCGCTTCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccggtcaggccggcccgaccgatctcttcgatgaccacggcgctgtagaggaagtccgcgccc                                                             >  2:2454651‑M1/1‑64 (MQ=255)
                                                                                        ttCGGCAGGTACTTGCGCTTCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccggtcaggccggcccgaccgatctcttcgatgaccacggcgctgtagaggaagtccgcgccca                                                            >  1:6872131‑M1/1‑63 (MQ=255)
                                                                                         tCGGCAGGTACTTGCGCTTCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccggtcaggccggcccgaccgatctcttcgatgaccacggcgctgtagaggaagtccgcgcccat                                                           <  2:7568224‑M1/151‑90 (MQ=255)
                                                                                           ggCAGGTACTTGCGCTTCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccggtcaggccggcccgaccgatctcttcgatgaccacggcgctgtagaggaagtccgcgcccatcc                                                         >  1:4616530‑M1/1‑60 (MQ=255)
                                                                                            gCAGGTACTTGCGCTTCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccggtcaggccggcccgaccgatctcttcgatgaccacggcgctgtagaggaagtccgcgcccatccc                                                        <  2:4488338‑M1/151‑93 (MQ=255)
                                                                                             cAGGTACTTGCGCTTCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccggtcaggccggcccgaccgatctcttcgatgaccacggcgctgtagaggaagtccgcgcccatcccg                                                       <  1:1536432‑M1/151‑94 (MQ=255)
                                                                                             cAGGTACTTGCGCTTCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccggtcaggccggcccgaccgatctcttcgatgaccacggcgctgtagaggaagtccgcgcccatcccg                                                       <  1:9811520‑M1/151‑94 (MQ=255)
                                                                                              aGGTACTTGCGCTTCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccggtcaggccggcccgaccgatctcttcgatgaccacggcgctgtagaggaagtccgcgcccatcccgc                                                      >  2:5807344‑M1/1‑57 (MQ=255)
                                                                                               ggTACTTGCGCTTCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccggtcaggccggcccgaccgatctcttcgatgaccacggcgctgtagaggaagtccgcgcccatcccgcc                                                     >  1:4749573‑M1/1‑56 (MQ=255)
                                                                                                   cTTGCGCTTCAGCGCCACGCTGCCTTAGTGGAGGATGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccggtcaggccggcccgaccgatctcttcgaagaccacgtcgctgtagaggaagtccgcgcccatcccgccatac                                                 >  2:11118540‑M1/1‑52 (MQ=255)
                                                                                                    ttGCGCTTCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccggtcaggccggcccgaccgatctcttcgatgaccacggcgctgtagagggagtccgcgcccatcccgccatact                                                >  1:4371154‑M1/1‑51 (MQ=255)
                                                                                                     tGCGCTTCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccggtcaggccggcccgaccgatctcttcgatgaccacggcgctgtagaggaagtccgcgcccatcccgccatactc                                               <  2:1245582‑M1/151‑102 (MQ=255)
                                                                                                     tGCGCTTCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccggtcaggccggcccgaccgatctcttcgatgaccacggcgctgtagaggaagtccgcgcccatcccgccatactc                                               >  2:7207442‑M1/1‑50 (MQ=255)
                                                                                                      gcgcTTCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccggtcaggccggcccgaccgatctcttcgatgaccacggcgctgtagaggaagtccgcgcccatcccgccatactct                                              >  1:2327969‑M1/1‑49 (MQ=255)
                                                                                                      gcgcTTCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgagcccggtcaggccggcccgaccgatctcttcgatgaccacggcgctgtagaggaagtccgcgcccatcccgccatactct                                              >  2:295046‑M1/1‑49 (MQ=255)
                                                                                                         cTTCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccggtcaggccggcccgaccgatctcttcgatgaccacggcgctgtagaggaagtccgcgcccatcccgccatactcttcc                                           <  1:1978148‑M1/151‑106 (MQ=255)
                                                                                                         cTTCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccggtcaggccggcccgaccgatctcttcgatgaccacggcgctgtagaggaagtccgcgcccatcccgccatactcttcc                                           >  2:3447464‑M1/1‑46 (MQ=255)
                                                                                                          ttCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccggtcaggccggcccgaccgatctcttcgatgaccacggcgctgtagaggaagtccgcgcccatcccgccatactcttccg                                          <  1:9122854‑M1/151‑107 (MQ=255)
                                                                                                           tCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccggtcaggccggcccgaccgatctcttcgatgaccacggcgctgtagaggaagtccgcgcccatcccgccatactcttccgg                                         >  1:6024521‑M1/1‑44 (MQ=255)
                                                                                                           tCAGCGCCTCGCTGCCGGAGGGGAGGAGGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccggtcaggccggcccgaccgatcggttcgatgaccacggcgctgtagaggaagtccgcgcccctcccgccatactcttccgg                                         >  2:6657312‑M1/1‑44 (MQ=255)
                                                                                                            cAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccggtcaggccggcccgaccgatctcttcgatgaccacggcgctgtagaggaagtccgcgcccatcccgccatactcttccggc                                        <  2:1482461‑M1/151‑109 (MQ=255)
                                                                                                              gcgcCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccggtcaggccggcccgaccgatctcttcgatgaccacggcgctgtagaggaagtccgcgcccatcccgccatactcttccggcag                                      <  1:751172‑M1/151‑111 (MQ=255)
                                                                                                                gcCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccggtcaggacggcccgaccgatctcttcgatgaccacggcgctgtagaggaagtccgcgcccatcccgccatactcttccggcaggt                                    <  2:6044935‑M1/151‑113 (MQ=255)
                                                                                                                 ccTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccggtcaggccggcccgaccgatctcttcgatgaccacggcgctgtagaggaagtccgcgcccatcccgccatactcttccggcaggtg                                   >  2:4715145‑M1/1‑38 (MQ=255)
                                                                                                                 ccTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccggtcaggccggcccgaccgatcccttcgatgaccacggcgctgtagaggaagtccgcgcccatcccgccatactcttccggcaggtg                                   <  2:6811404‑M1/151‑114 (MQ=255)
                                                                                                                  cTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccggtcaggccggcccgaccgatctcttcgatgaccacggcgctgtagaggaagtccgcgcccatcccgccatactcttccggcaggtgc                                  <  1:8386675‑M1/151‑115 (MQ=255)
                                                                                                                  cTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccggtcaggccggcccgaccgatctcttcgatgaccacggcgctgtagaggaagtccgcgcccatcccgccatactcttccggcaggtgc                                  <  2:6475987‑M1/151‑115 (MQ=255)
                                                                                                                     gCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccggtcaggccggcccgaccgatctcttcgatgaccacggcgctgtagaggaagtccgcgcccatcccgccatactcttccggcaggtgcgaa                               <  1:9628381‑M1/151‑118 (MQ=255)
                                                                                                                           gTAGTGGAGGATGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccggtcaggccggcccgaccgatctcttcgatgaccacggcgctgtagaggaagtccgcgcccatcccgccatactcttccggcaggtgcgaacagagc                         >  1:7735482‑M1/1‑28 (MQ=255)
                                                                                                                            tAGTGGAGGATGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccggtcaggccggcccgaccgatctcttcgatgaccacggcgctgtagaggaagtccgcgcccatcccgccatactcttccggcaggtgcgaacagagca                        <  1:1207595‑M1/151‑125 (MQ=255)
                                                                                                                                ggaggaTGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccggtcaggccggcccgaccgatctcttcgatgaccacggcgctgtagaggaagtccgcgcccatcccgccatactcttccggcaggtgcgaacagagcattcc                    <  2:3662135‑M1/151‑129 (MQ=255)
                                                                                                                                ggaggaTGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccggtcaggccggcccgaccgatctcttcgatgaccacggcgctgtagaggaagtccgcgcccatcccgccagactcttccggcaggtgcgaacagagcattcc                    >  1:4973099‑M1/1‑23 (MQ=255)
                                                                                                                                 gaggaTGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccggtcaggccggcccgaccgatctcttcgatgaccacggcgctgtagaggaagtccgcgcccatcccgccatactcttccggcaggtgcgaacagagcattccg                   >  2:9026191‑M1/1‑22 (MQ=255)
                                                                                                                                  aggaTGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccggtcaggccggcccgaccgatctcttcgatgaccacggcgctgtagaggaagtccgcgcccatcccgccatactcttccggcaggtgcgaacagagcattccgg                  >  2:2166414‑M1/1‑21 (MQ=255)
                                                                                                                                  aggaTGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccggtcaggccggcccgaccgatctcttcgatgaccacggcgctgtagaggaagtccgcgcccatcccgccatactcttccggcaggtgcgaacagagcattccgg                  <  2:2091401‑M1/151‑131 (MQ=255)
                                                                                                                                    gaTGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccggtcaggccggcccgaccgatctcttcgatgaccacggcgctgtagaggaagtccgcgcccatcccgccatactcttccggcaggtgcgaacagagcattccggcc                >  2:8931783‑M1/1‑19 (MQ=255)
                                                                                                                                     aTGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccggtcaggccggcccgaccgatctcttcgatgaccacggcgctgtagaggaagtccgcgcccatcccgccatactcttccggcaggtgcgaacagagcattccggcct               <  1:6411682‑M1/151‑134 (MQ=255)
                                                                                                                                      tGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccggtcaggccggcccgaccgatctcttcgatgaccacggcgctgtagaggaagtccgcgcccatcccgccatactcttccggcaggtgcgaacagagcattccggcctc              >  2:4165260‑M1/1‑17 (MQ=255)
                                                                                                                                      tGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccggtcaggccggcccgaccgatctcttcgatgaccacggcgctgtagaggaagtccgcgcccatcccgccatactcttccggcaggtgcgaacagagcattccggcctc              <  1:3539046‑M1/151‑135 (MQ=255)
                                                                                                                                      tGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccggtcaggccggcccgaccgatctcttcgatgaccacggcgctgtagaggaagtccgcgcccatcccgccatactcttccggcaggtgcgaacagagcattccggcctc              <  2:69677‑M1/151‑135 (MQ=255)
                                                                                                                                      tGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccggtcaggccggcccgaccgatctcttcgatgaccacggcgctgtagaggaagtccgcgcccatcccgccatactcttccggcaggtgcgaacagagcattccggcctc              <  1:9452552‑M1/151‑135 (MQ=255)
                                                                                                                                       gTAGGGCGCAGCGATGtccgaatgcagtgagaaaccgatcccggtcaggccggcccgaccgatctcttcgatgaccacggcgctgtagaggaagtccgcgcccatcccgccatactcttccggcaggtgcgaacagagcattccggcctcg             <  2:10376205‑M1/151‑136 (MQ=255)
                                                                                                                                        tAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccggtcaggccggcccgaccgatctcttcgatgaccacggcgctgtagaggaagtccgcgcccatcccgccatactcttccggcaggtgcgaacagagcattccggcctcgc            >  2:1969444‑M1/1‑15 (MQ=255)
                                                                                                                                          gggCGCAGCGATGtccgaatgcagggagaaaccgatcccggtcaggccggcccgaccgatctcttcgatgaccacggcgctgtagaggaagtccgcgcccatcccgccatactcttccggcaggtgcgaacagagcattccggcctcgccg          <  1:11085661‑M1/151‑139 (MQ=255)
                                                                                                                                           ggCGCAGCGATGtccgaatgcagggagaaaccgatcccggtcaggccggcccgaccgatctcttcgatgaccacggcgctgtagaggaagtccgcgcccatcccgccatactcttccggcaggtgcgaacagagcattccggcctcgccgg         >  2:6312771‑M1/1‑12 (MQ=255)
                                                                                                                                           ggCGCAGCGATGtccgaatgcagggagaaaccgatcccggtcaggccggcccgaccgatctcttcgatgaccacggcgctgtagaggaagtccgcgcccatcccgccatactcttccggcaggtgcgaacagagcattccggcctcgccgg         <  2:8472247‑M1/151‑140 (MQ=255)
                                                                                                                                           ggCGCAGCGATGtccgaatgcagggagaaaccgatcccggtcaggccggcccgaccgatctcttcgatgaccacggcgctgtagaggaagtccgcgcccatcccgccatactcttccggcaggtgcgaacagagcattccggcctcgccgg         >  1:3679202‑M1/1‑12 (MQ=255)
                                                                                                                                              gcAGCGATGtccgaatgcagggagaaaccgatcccggtcaggccggcccgaccgatctcttcgatgaccacggcgctgtagaggaagtccgcgcccatcccgccatactcttccggcaggtgcgaacagagcattccggcctcgccggcct      <  1:6706877‑M1/151‑143 (MQ=255)
                                                                                                                                               cAGCGATGtgcgaatgcagggagaaaccgatcccggtcaggccggcccgaccgatctcttcgatgaccacggcgctgtagaggaagtccgcgcccatcccgccatactcttacggcaggtgcgaacagagcattccggcctcgccggcctt     >  2:5090306‑M1/1‑8 (MQ=255)
                                                                                                                                               cAGCGATGtccgaatgcagggagaaaccgatcccggtcaggccggcccgaccgatctcttcgatgaccacggcgctgtagaggaagtccgcgcccatcccgccatactcttccggcaggtgcgaacagagcattccggcctcgccggcctt     >  1:8111977‑M1/1‑8 (MQ=255)
                                                                                                                                                atccatgtccgaatgcagggagaaaccgatcccggacaggcccgccagacagatctcttcgaagaccacggcgctgaagaggaagtccgctcccatcccgacatactcttcctgcaggtgcgaacagagcattacggcctcgccgaccttg    >  2:9132167‑M1/5‑7 (MQ=255)
                                                                                                                                                aGCGCTGtccgaatgcagggagaaaccgatcccggtcaggccggcccgaccgatctcttcgatgaccacggcgctgtagaggaagtccgcgcccatcccgccatactcttccggcaggtgcgaacagagcattccggcctcgccggccttg    <  1:7219701‑M1/151‑145 (MQ=255)
                                                                                                                                                aGCGATGtccgaatgcagggagaaaccgatcccggtcaggccggcccgaccgatctcttcgatgaccacggcgctgtagaggaagtccgcgcccatcccgccatactcttccggcaggtgcgaacagagcattccggcctcgccggccttg    >  2:5395146‑M1/1‑7 (MQ=255)
                                                                                                                                                  cGATGtccgaatgcagggagaaaccgatcccggtcaggccggcccgaccgatctcttcgatgaccacggcgctgtagaggaagtccgcgcccatcccgccatactcttccggcaggtgcgaacagagcattccggcctcgccggccttgtt  >  2:6548686‑M1/1‑5 (MQ=255)
                                                                                                                                                  cGATGtccgaatgcagggagaaaccgatcccggtcaggccggcccgaccgatctcttcgatgaccacggcgctgtagaggaagtccgcgcccatcccgccatactcttccggcaggtgcgaacagagcattccggcctcgccggccttgtt  <  2:8206882‑M1/151‑147 (MQ=255)
                                                                                                                                                      |                                                                                                                                                  
TACCGCCGTGGTCTTCACCCCTTGCAGGTCGGAACCGGCCCCCGGCTCGGTCATGGCGATGGCCGCGACCATCTCGCCGGATACCAGCTTCGGCAGGTACTTGCGCTTCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGGCGCAGCGATGTCCGAATGTAGGGCGCAGCGATGTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCCCGACCGATCTCTTCGATGACCACGGCGCTGTAGAGGAAGTCCGCGCCCATCCCGCCATACTCTTCCGGCAGGT  >  NC_008463/5131578‑5131874

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑