Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084634,616,4540TG32.4% 246.1 / 27.8 139intergenic (‑174/+28)hda/PA14_RS21180DnaA regulatory inactivator Hda/DUF2066 domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base T (52/40);  new base G (42/2);  total (97/42)
Fisher's exact test for biased strand distribution p-value = 1.00e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 144 total aligned reads displayed.

AAACGCCGCAGGAGTCACCGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGC‑GCC‑GGCCCGCT‑CC‑GTT‑GCATAGGAGC‑GGATCGGCGCGCCGACTCACTG‑CCA‑G‑C‑GG‑AAATTCAGTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTACGCTGTCGGCCGGGATTTCCTGCAACTGCGCCAGGCCCA  >  NC_008463/4616304‑4616603
                                                                                                                                                          |                                                                                                                                                            
 accgccgcAGGAGTCACCGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGC‑GCC‑GGCCCGCT‑CC‑Gtt                                                                                                                                                             <  1:3725639/149‑1 (MQ=255)
       gcAGGAGTCACCGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGG‑GGG‑GGGCCGCG‑CC‑GGT‑ggggag                                                                                                                                                      >  1:8163884/1‑146 (MQ=255)
       gcAGGAGTCACCGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGG‑GCC‑GGCCCCCC‑CC‑GGT‑Gcgatg                                                                                                                                                      >  1:3944327/1‑147 (MQ=255)
        cAGGAGTCACCGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCACAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGC‑GCC‑GGCCCGCT‑CC‑GTT‑GCATAgg                                                                                                                                                     <  1:5166415/151‑1 (MQ=255)
        cAGGAGTCACCGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGC‑GCC‑GGCCCGCA‑CC‑CTT‑gacaagg                                                                                                                                                     >  2:3641794/1‑145 (MQ=255)
         aGGAGTCACCGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGG‑GGC‑GGGCCCCC‑CC‑GGT‑Gcggaggg                                                                                                                                                    >  1:8404602/1‑145 (MQ=255)
          ggAGTCACCGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGC‑GGC‑GGCCCCCG‑CC‑CGT‑GCAAAggtg                                                                                                                                                   >  2:7965295/1‑149 (MQ=255)
          ggAGTCACCGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGC‑GCC‑GGCCCGCT‑CC‑GTT‑GCATAGGAg                                                                                                                                                   <  2:5759963/151‑1 (MQ=255)
          ggAGTCACCGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGC‑GCC‑GGCCCGCC‑CC‑GCT‑GGCTAgggg                                                                                                                                                   >  1:6031191/1‑149 (MQ=255)
           gAGTCACCGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGC‑GCC‑GGCCCGCT‑CC‑GTT‑GCATAGGAGc                                                                                                                                                  <  1:8099902/151‑1 (MQ=255)
             gTCACCGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGC‑GCC‑CGCGCCCT‑CC‑GGT‑GCCTAgggtcgg                                                                                                                                                >  1:7422524/1‑146 (MQ=255)
               cACCGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGC‑GCC‑GGCCCGCT‑CC‑GTT‑GCATAGGAGC‑GGAt                                                                                                                                             <  1:10032640/151‑1 (MQ=255)
                aCCGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGC‑GCC‑GGCCCGCG‑CC‑GGT‑GCATAGGTGC‑GGATc                                                                                                                                            >  2:6329617/1‑151 (MQ=255)
                 ccGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGC‑GCC‑GGCCCGCT‑CC‑GTT‑GCATAGGAGC‑GGATCg                                                                                                                                           <  1:1775156/151‑1 (MQ=255)
                   gTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCACAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGC‑GCC‑GGCCCGCT‑CC‑GTT‑GCATAGGAGC‑GGATCGgc                                                                                                                                         <  2:6662718/151‑1 (MQ=255)
                     aaaGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAACGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGC‑GCC‑GGCCCGCT‑CC‑GTT‑GCATAGGAGC‑GGATCGgcgc                                                                                                                                       <  2:4187130/151‑1 (MQ=255)
                     aaaGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGG‑GGC‑GGGCCCCC‑CC‑CGT‑GCCAATGAGC‑GGagccgggc                                                                                                                                       >  1:7706840/1‑144 (MQ=255)
                     aaaGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGC‑GCC‑GGCCCGCT‑CC‑GTT‑GCATAGGAGC‑GGATCGgcgc                                                                                                                                       <  2:7275832/151‑1 (MQ=255)
                         gCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGC‑GCC‑GGCCCGCT‑CC‑GTT‑GCATAGGAGC‑GGATCGGCGCGCCg                                                                                                                                   <  2:3994103/151‑1 (MQ=255)
                           tGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGCTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGACGCGC‑GCC‑GGCCCGCT‑CC‑GTT‑GCATAGGAGC‑GGATCGGCGCGCCGAc                                                                                                                                 <  2:1739001/151‑1 (MQ=255)
                             cAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGC‑GCC‑CGCCCCCG‑CC‑GGT‑TGAGATGAGC‑GGGTCGGGGCGcccccac                                                                                                                               >  2:1997325/1‑146 (MQ=255)
                             cAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGC‑CGG‑GGCCCCCG‑CC‑CGT‑GGAGAGGGGC‑GGGTAGgcgcggccccgc                                                                                                                               >  2:1997765/1‑144 (MQ=255)
                                aGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGG‑GCC‑CGCCCCCG‑CC‑CTT‑GGAAATGAGC‑GGGACGGCGCGCCggcaaaca                                                                                                                            >  2:9585946/1‑144 (MQ=255)
                                aGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGC‑GCC‑GGGCCGCG‑CCGGTG‑GCAT‑GGCGC‑GGGTCGGCCGGGCGCCTCAcg                                                                                                                            >  2:8661493/1‑150 (MQ=255)
                                aGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGC‑GCC‑GGCCCGCG‑CCGGTT‑GAAT‑GGTGC‑GGCTCGGCGCGCCGGCTCAcg                                                                                                                            >  2:9585924/1‑150 (MQ=255)
                                 gTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGC‑GGC‑GGGCCGCG‑CCGGTT‑GC‑TTCGAGC‑GGATCGGCGCGCCGACTCACTg                                                                                                                           >  2:2642146/1‑151 (MQ=255)
                                     ataCGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGG‑GCC‑CGCCCCCG‑CC‑CGT‑GGGAAGGCGC‑GGGTCGGGGGGCCGCCCCACAG‑cccc                                                                                                                      >  1:10160896/1‑149 (MQ=255)
                                      taCGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCTG‑GCC‑GGCCCGCT‑CC‑ATT‑TCATAGGAGC‑GGGTCGGGTGGCCGCCGCAcag‑cca‑g‑c                                                                                                                   >  2:3639502/1‑151 (MQ=255)
                                      taCGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGG‑GCC‑GGCCCGCG‑GC‑GGT‑TCAGAGGTGC‑GGATCGGGGCGGCGACTCACGG‑ccccc                                                                                                                     >  2:6446333/1‑148 (MQ=255)
                                      taCGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGC‑GCC‑GGCCCGCG‑CC‑CGT‑GGAAATGGGC‑GGGTCGGCGCGCCGACGCACGG‑CCA‑G‑c                                                                                                                   >  2:6450273/1‑151 (MQ=255)
                                         gCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGC‑GCC‑GGGCCGCC‑CC‑GTT‑GGCGAGGAGC‑GGATCGGGGCGCCGACGCACGG‑CCC‑G‑C‑GG‑a                                                                                                              >  2:5803769/1‑151 (MQ=255)
                                          ccTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGG‑GGC‑CGGCCGCG‑CC‑CGT‑GCAAAGGAGC‑GGGTCGGCGCGCCGCCACACAG‑cccccggga                                                                                                                 >  2:5100064/1‑144 (MQ=255)
                                           cTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGCGGCC‑GGCCCCGC‑CC‑GTG‑GGA‑ATGAGC‑GGTTCGGCGCGCCGCCACACAG‑CCA‑C‑C‑GG‑gaa                                                                                                            >  2:7704890/1‑151 (MQ=255)
                                           cTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGAGAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGG‑GGC‑GGCCCGCA‑CC‑GGT‑GGAGGGGAGC‑GGCTGGGCGCGCCGCCTCACAG‑ACA‑G‑C‑GG‑gaa                                                                                                            >  2:5886124/1‑151 (MQ=255)
                                             cGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGAGG‑GCC‑GGGCCGCG‑AC‑GTT‑TGTTTGGGGG‑GGCTAGGCGCGGCGAAACACGG‑GCA‑G‑C‑GG‑aaaat                                                                                                          >  2:4287984/1‑149 (MQ=255)
                                              ggCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGC‑GGC‑CGGCCGCG‑CC‑GGG‑GCCGAGGAGA‑GGGTAGGCGGGCCCCCACACAG‑CCA‑C‑C‑GG‑CAAATc                                                                                                         >  2:4811824/1‑151 (MQ=255)
                                                          caATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGC‑CCC‑CGCCCGCG‑CC‑CGT‑GCCTAAGAGC‑CGGAAGGCCCGCCCCCACACAT‑CCA‑G‑C‑CG‑CAAATAAGTTggggagac                                                                                             >  2:8929089/1‑146 (MQ=255)
                                                          caATGACAAACTTGTCAACAAAAATAAAAGGTTGGCCTATCGCCGTGGCTCCTTGCCCAGCGCAGGGGCGGGTCGCGC‑GCC‑GGCCCGCT‑CG‑GTT‑GCATCGGCGC‑GCATCGGCCCCCCGACTCACTG‑CCA‑G‑C‑GG‑AAATTCAGTTGGgacgac                                                                                             <  2:7335368/151‑1 (MQ=255)
                                                           aaTGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGC‑GGC‑GGCCCCCT‑CC‑GTT‑GCATATGTTC‑GGATCGGCGCGCCGACGCACAG‑CCA‑G‑CGGG‑AAATTAGGTTgggcggcc                                                                                             >  2:9663619/1‑146 (MQ=255)
                                                                gCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGC‑GCC‑CGCCCGCT‑CC‑CCT‑GGCGATGGGC‑GGCTCGGCGCGGCCACACACAG‑GCC‑G‑C‑GG‑GAAATAAGTTTGGACGACCACCGc                                                                                       >  2:207512/1‑151 (MQ=255)
                                                                 caccTAGCCAAACAAAATCTACGGTTCGCCGACCCCCGAGCCACCGTGCCCCCCCCTTCGGCGGGCCGCCC‑GCC‑CGCCCGCG‑CG‑GTT‑GATTCGTCGC‑GGATGGGCGCGCCGACTCACTG‑CCA‑G‑C‑GG‑AAATTCAGTTGGGACGACGACGGCa                                                                                      <  2:6240399/148‑1 (MQ=255)
                                                                  aaCTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGC‑GCC‑GGGCCGCT‑CC‑CGT‑GCAAAAGGTC‑GGCTAGGCGCGCCGGCACACAG‑CCA‑C‑C‑GC‑GAAATCAGTGGGGACGACGACGGCAc                                                                                     >  2:11190298/1‑151 (MQ=255)
                                                                   aCTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGC‑GCC‑GGGCCGCTCCC‑GTT‑CCA‑AGGAGC‑GGATCCGCGCGCCGTCGCACAG‑GCA‑GCC‑GG‑CAATAAGGTTGGG‑CGACGACGGCAcc                                                                                    >  2:11200248/1‑151 (MQ=255)
                                                                     tAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCCTCGGCCGGTCGCGC‑GCC‑GGCCCCCC‑CC‑GGT‑GCAAGAGAGC‑GGTCCGGCGCCCCCCCGGACGG‑CCA‑G‑C‑GGCAAAAACAG‑TGGGTAGAAGAAGGCAccgc                                                                                  >  2:9852431/1‑151 (MQ=255)
                                                                     tAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGC‑GCC‑CGCCCCCTCCC‑GTT‑GC‑GAGGAGC‑GGCTGGGCGCGCCCCCTCACGG‑CCC‑C‑C‑GG‑AAAATAAGGTGGGGCGACGACGGCAcccc                                                                                  >  2:1672161/1‑149 (MQ=255)
                                                                              aaaaTCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGG‑GCC‑GGGCCCCG‑CC‑CGT‑GGATATGTGG‑GGATTCGGGCGCCGCCGCAACG‑ACC‑C‑C‑GG‑AAATAAATTTGGGGCGACCACGGCCCCCCCGCGCgggg                                                                         >  1:2645291/1‑151 (MQ=255)
                                                                              aaaaTCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGC‑GCC‑GGCCCGCA‑CC‑GTT‑GCATAGGTGC‑GGATCCGCGCGCCGACTCACAT‑CCA‑G‑C‑GG‑AAAATAATTTTGGACCACGACGGCACCGCCGCGCgggg                                                                         >  2:9930858/1‑151 (MQ=255)
                                                                               aaaTCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGG‑GCC‑GGCCCCCG‑CC‑CGT‑GGAAATGAGC‑GGCTAGGGGCCCCGAAACAAAA‑CCACC‑C‑GG‑AAATAAAGTT‑GGACGACGACGGCAACGCCCCCCGGGgc                                                                        >  2:2283755/1‑151 (MQ=255)
                                                                                             ccGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGG‑GCC‑CGGCCGCG‑AC‑GGT‑GGGTAGGTGC‑GGTAGGGCGGGCCCGCACACAT‑CCA‑A‑C‑GG‑GAAATGAGTTTGGTCCACCACCGGAACCCCCCGCGGGGGGCGGCGGGGTgggc                                                          >  2:5662414/1‑149 (MQ=255)
                                                                                             ccGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGG‑GCC‑CGCCCGCA‑CC‑GGT‑GGGAAGGAGC‑GGGTCGGGGCGCCGCCGCACAG‑CCA‑C‑C‑CG‑CAAAAAACGTTGGAGGACCACGGCACCCCCCCGCGGGGCGCGGCGGGCTGGcc                                                          >  2:7611300/1‑151 (MQ=255)
                                                                                              cgtgcgCCGAGTCTCCTTCCCCTGTGCTTCGGCGGGGCGCGC‑CCC‑GGCCCGCT‑CC‑TTT‑GCATTGGTGC‑GGTTGGGCCCGCCGACTCACTG‑CCA‑G‑C‑GG‑AAATTCAGTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCg                                                         <  2:3322629/146‑1 (MQ=255)
                                                                                              cGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGC‑GCC‑GGCCCGCG‑CC‑GGT‑GCAATGGTCC‑GGATCGCGGCGCCGACTAACTG‑CCA‑G‑C‑GG‑CAAGTCACTTTGGACGACGACCGCACCGCCCCGCGGGGCGCGGCCGGCTGGccc                                                         >  2:4487053/1‑150 (MQ=255)
                                                                                               gATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGG‑GCC‑CGGCCCCA‑CC‑GGT‑GGCGAGGCGG‑GGGTCGGGGGGCCGGCGCACCG‑CCC‑G‑C‑GG‑GCAATGAGGTTGGGGGAGGGCGGGTCCCCCGCGCTGGGCGCGGCGGGGTGGGCgg                                                        >  2:6722617/1‑151 (MQ=255)
                                                                                                aTAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGG‑GCC‑CGCCCCCA‑CC‑GGG‑GGATAGGAGA‑GGATGGGGGGGCCCAAAAACAG‑ACA‑G‑C‑GG‑TAAATAAGTTTTGGCGACGACGGCACCGCCCCGCAGGGGGCGAGGGGCTGGCCggt                                                       >  2:1581431/1‑150 (MQ=255)
                                                                                                  aGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGG‑GCCGGGCCCCGC‑CC‑GTT‑GGA‑GTGAGCGGGGGCGGGGC‑CCCCCCCAAAG‑CCC‑G‑G‑GG‑GACTTAAGTTGTGGCGACGACGGCACCCCCGCGCGGGGCGGGGGGGGCTGGGCGgggg                                                     >  2:4755324/1‑151 (MQ=255)
                                                                                                    ccGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGC‑GCC‑CGCCCGCG‑CC‑GTT‑GGATAGGAGC‑GGCTCGGCGCGCCGCCGCACGG‑CCC‑G‑C‑GG‑TACTTAAGGTGGGACGACCACCGGCCCCCCGCGCGGGGCGCGGGGGGCTGGCCGGGGGcc                                                   >  2:8056748/1‑150 (MQ=255)
                                                                                                      gAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGG‑GCC‑GGCCCGCG‑CC‑CTT‑GGAGTGGGGC‑GGCTCGGCGCGCCGGCGCACGG‑CCC‑G‑C‑GG‑GACTTCGGGTGGGGCGGCGACGGGGCCGCCGCGCGGGGGGCGGGGGGCGGGGCGGGGGCCTc                                                 >  2:7253382/1‑151 (MQ=255)
                                                                                                         gCTCCTTGCCCAGAGCATCGGCCGGTCGCGG‑GCC‑GGCCCCCG‑CC‑GGT‑GGGTTGGAGC‑GGGTCGGCGCGCCCCCTCACAGCCCA‑C‑C‑GG‑CAATAAAGTTGGG‑CGCCGAAGGCCCCCCCCCGCGGGGGGCGGCGGGCGGGGCGgggggaggccc                                              >  2:5143190/1‑144 (MQ=255)
                                                                                                         gCTCCTTGCCCAGAGCATCGGCCGGTCGCGC‑GGC‑GGGCCCCG‑CC‑GGT‑TCAAAGGAGC‑GGAACGGCGCCCCCACAGAAAG‑CCC‑G‑C‑GG‑TAATTAAGTTGGGGCGTCCACGGCACCGCCCCGCAGGGGGCTGAGGGCAGGCCGGAGGCAACCac                                              >  2:1823042/1‑150 (MQ=255)
                                                                                                            ccTTGCCCAGAGCATCGGCCGGTCGCGG‑GGC‑GGGCCCCC‑CC‑GGT‑GCCGAGGGGC‑GGTGCGGCGCGCCGACACCACG‑CCC‑A‑C‑GG‑GCAATGACTTGGGGCGACGACGGCGCCGCCCCGCCGGGCGCGTGGGGCGGGCCGGGGGCCTCCACCgg                                           >  2:9747065/1‑151 (MQ=255)
                                                                                                             ctgccccAGCGCTCCGGCCGGGCGCCC‑GCC‑GGCCCGCG‑CG‑GTG‑CCAGCGGTGC‑GGCGCGGCGCCCCGACTCACTG‑CCA‑G‑C‑GG‑AAATTCAGTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGt                                          <  2:7795654/147‑1 (MQ=255)
                                                                                                              cgccccAGACCCTCGGCCGGCCGCGC‑GCC‑GCCCCGCT‑CC‑GGT‑GCCTCGGAGC‑GGCGCGGCGCCCCGACTCACTG‑CCA‑G‑C‑GG‑AAATTCAGTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTa                                         <  2:9771992/148‑1 (MQ=255)
                                                                                                               tGCCCAGTGAATGGGCGGGTCGCGC‑GCC‑GGCCCGCG‑CG‑TTT‑TCTTTGTTGG‑GTTGCGGCGCGCCGACTCACTG‑CCA‑G‑C‑GG‑AAATTCAGTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTAc                                        <  2:8207740/151‑1 (MQ=255)
                                                                                                                  ccAGAGCATCGGCCGGTCGCGG‑GCC‑GGCCCCCC‑CC‑GGT‑GCCTAAGAGC‑CGATAGGCGCGCCCACACACAC‑CCC‑C‑C‑CG‑CAATTACGTATTGGCGACCAAGGCCCCGCCCCCCGGGGCGCGGCGGGCGGGCCGGGGGCCTACAGCgggtagcg                                     >  2:1194753/1‑145 (MQ=255)
                                                                                                                   ctgaccttCGGCCGGCCGCGC‑GCC‑GCCCCGCT‑CC‑GTG‑GAATCGGCGG‑GGCTGGGCGCCCCGACTCACTG‑CCA‑G‑C‑GG‑AAATTCAGTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTACGCTg                                    <  2:7479092/144‑1 (MQ=255)
                                                                                                                   cAGAGCTTCGGGCGGCCGGCC‑GCC‑GGCCCGCT‑CC‑TTT‑GATTCGTTGC‑GTTTCGCCCCCCCGACTCACTG‑CCA‑G‑C‑GG‑AAATTCAGTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTACGCTg                                    <  1:8069841/151‑1 (MQ=255)
                                                                                                                     gccctggggCGGGGCGCGC‑GCC‑GCCCCGCG‑CG‑GTG‑CTTTGGGCGC‑GGCGGGGCGCCCCGACTCACTG‑CCA‑G‑C‑GG‑AAATTCAGTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTACGCTGTc                                  <  1:2419269/144‑1 (MQ=255)
                                                                                                                     gcccccgggCCGGTCGCCC‑GCC‑GGCCCGCC‑CC‑GTG‑TAATCGGCGG‑GCTTCGGCGCCCCGACTCACTG‑CCA‑G‑C‑GG‑AAATTCAGTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTACGCTGTc                                  <  2:861556/144‑1 (MQ=255)
                                                                                                                       cccgcGGCCGGGCGCGC‑GCC‑GCCCCGC‑‑CC‑GTTGGCTTAGGCGG‑GGACGGGCCCCCCGACTCACTG‑CCA‑G‑C‑GG‑AAATTCAGTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTACGCTGTCgg                                <  1:6448234/147‑1 (MQ=255)
                                                                                                                            gggggggcgccc‑gCG‑GCCCCGCT‑CC‑TTT‑CCTTCGGGGG‑GGTTGGGCGCCCCGACTCACTG‑CCA‑G‑C‑GG‑AAATTCAGTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTACGCTGTCGGCCggg                           <  1:10847599/144‑1 (MQ=255)
                                                                                                                               cggggccgc‑gcC‑GGCCCCCG‑CC‑GTT‑CCTTCGGTGC‑GGTTGGGCGCCCCGACTCACTG‑CCA‑G‑C‑GG‑AAATTCAGTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTACGCTGTCGGCCGGGAtt                        <  2:11187426/145‑1 (MQ=255)
                                                                                                                                 gTCGCGC‑GCC‑GGCCCGCT‑CC‑GGT‑GCATAGGAGC‑GGATCGGCGCGCCGACTCACTG‑CCA‑G‑C‑GG‑AAAATCAGTTGGGACGACGACGGCACCGCCGCGCGGGGCGCTGCGGGCTGGCCGGCGGCATCCAGCGGGACGCGGTCGGCCGGGATTTc                      >  2:5402109/1‑151 (MQ=255)
                                                                                                                                       c‑gcC‑GGCCCGCT‑CC‑GTT‑GCATAGGAGC‑GGATCGGCGCGCCGACTCACTG‑CCA‑G‑C‑GG‑AAATTCAGTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTACGCTGTCGGCCGGGATTTCCTGCaa                >  2:605663/1‑151 (MQ=255)
                                                                                                                                         gcC‑GGCCCGCT‑CC‑GTT‑GCATAGGAGC‑GGTTCGGCGCGCCGACTCACTG‑CCA‑G‑C‑GG‑AAATTCAGTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTACGCTGTCGGCCGGGATTTCCTGCAAc               >  1:8244700/1‑151 (MQ=255)
                                                                                                                                          cc‑gccccgcc‑CC‑GTT‑CCAGCGGCGC‑GGACCGGCGCCCCGACTCACAG‑CCA‑G‑C‑GG‑AAATTCAGTTGGGACGCCGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTACGCTGTCGGCCGGGATTTCCTGCAAcc              <  1:3517235/151‑2 (MQ=255)
                                                                                                                                          cc‑GGCCCGCT‑CC‑GTT‑GCATAGGAGC‑GGATCGGCGCGCCGACTCACTG‑CCA‑G‑C‑GG‑AAATTCAGTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCCGCGGTCCGCTGTCGGCCGGGCTTTCCTGCAAcg              >  1:6227066/1‑150 (MQ=255)
                                                                                                                                           c‑GGCCCGCT‑CC‑GTT‑GCATAGGAGC‑GGATCGGCGCGCCGACTCACTG‑CCA‑G‑C‑GG‑AAATTCAGTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTACGCTGTCGGCCGGGATTTCCTGCAACTg             >  2:2301011/1‑151 (MQ=255)
                                                                                                                                            cgccccGCT‑CG‑GTG‑TCATCGGCGC‑GGACGGGCGCCCCGACTCACTG‑CCA‑G‑C‑GG‑AAATTCAGTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTACGCTGTCGGCCGGGATTTCCTGCAACTg             <  1:11197641/148‑1 (MQ=255)
                                                                                                                                             ggCCCGCT‑CC‑GTT‑GCATAGGAGC‑GGATCGGCGCGCCGACTCACTG‑CCA‑G‑C‑GG‑AAATTCAGTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTACGCTGTCGGCCGGGATTTCCTGCAACTgc            >  2:9197819/1‑151 (MQ=255)
                                                                                                                                              gCCCGCT‑CC‑GTT‑GCAGAGGAGC‑GGATCGGCGCGCCGACTCACTG‑CCA‑G‑C‑GG‑CCCTTCCGTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGCGGCATCGAGCGGAACGCTGTCGGCCGGGCTTTCGTGCAACTgcg           >  2:7570044/1‑151 (MQ=255)
                                                                                                                                               cccGCT‑CC‑GTT‑GCATAGGAGC‑GGATCGGCGCGCCGACTCACTG‑CCC‑G‑C‑GG‑AAATTCAGTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTACGCTGTCGGCCGGGATTTCCTGCAACTgcgc          >  1:8572340/1‑151 (MQ=255)
                                                                                                                                               cccGCT‑CC‑GTT‑GCATAGGAGC‑GGATCGGCGCGCCGACTCACTG‑CCA‑G‑C‑GG‑AAATTCAGTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTACGCTGTCGGCCGGGATTTCCTGCAACTgcgc          >  1:8572336/1‑151 (MQ=255)
                                                                                                                                               cccGCT‑CC‑GTT‑GCATAGGAGC‑GGATCGGCGCGCCGACTCACTG‑CCA‑G‑C‑GG‑AAATTCAGTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTACGCTGTCGGCCGGGATTTCCTGCAACTgcgc          >  1:499493/1‑151 (MQ=255)
                                                                                                                                                ccGCT‑CC‑TGT‑GCCTAGGAGC‑GGAGCGCCGCGCCGACTCACGG‑CCA‑G‑C‑GG‑AAATTCAGGTGTGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTACGCTGTCGGCCGGGATTTCCTGCAACTGCGcc         <  2:4323255/151‑1 (MQ=255)
                                                                                                                                                ccGCT‑CC‑GTT‑GCATAGGAGC‑GGATCGGCGCGCCGACTCACTG‑CCA‑G‑C‑GG‑AAATTCAGTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTACGCTGTCGGCCGGGATTTCCTGCAACTGCGcc         >  1:10205278/1‑151 (MQ=255)
                                                                                                                                                ccGCT‑CC‑GTT‑GCATAGGAGC‑GGATCGGCGCGCCGACTCACTG‑CCA‑G‑C‑GG‑AAATTCAGTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTACGCTGTCGGCCGGGATTTCCTGCAACTGCGcc         >  2:3219029/1‑151 (MQ=255)
                                                                                                                                                 cGCT‑CT‑GTT‑GCTTCGGTGC‑GTAGCGGCGCCCCGACTCACTG‑CCA‑G‑C‑GG‑AAATTCAGTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTACGCTGTCGGCCGGGATTTCCTGCAACTGCGCCa        <  2:1300421/151‑1 (MQ=255)
                                                                                                                                                 cGCT‑CC‑GTT‑GCATAGGAGC‑GGATCGGCGCGCCGACTCACTG‑CCA‑G‑C‑GG‑AAATTCAGTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTACGCTGTCGGCCGGGATTTCCTGCAACTGCGCCa        >  2:9949354/1‑151 (MQ=255)
                                                                                                                                                 cGCT‑CC‑GTT‑GCATAGGAGC‑GGATCGGCGCGCCGACTCACTG‑CCA‑G‑C‑GG‑AAATTCAGTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTACGCTGTCGGCCGGGATTTCCTGCAACTGCGCCa        >  1:7924346/1‑151 (MQ=255)
                                                                                                                                                  gCT‑CC‑GTT‑GCATAGGAGC‑GGATCGGCGCGCCGACTCACTG‑CCA‑G‑C‑GG‑AAATTCAGTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTACGCTGTCGGCCGGGATTTCCTGCAACTGCGCCAg       >  2:8267245/1‑151 (MQ=255)
                                                                                                                                                  gCT‑CC‑GTT‑GCATAGGAGC‑GGATCGGCGCGCCGACTCACTG‑CCA‑G‑C‑GG‑AAATTCAGTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTACGCTGTCGGCCGGGATTTCCTGCAACTGCGCCAg       >  2:2629931/1‑151 (MQ=255)
                                                                                                                                                   cT‑CC‑GTT‑GCATAGGAGC‑GGATCGGCGCGCCGACTCACTG‑CCA‑G‑C‑GG‑AAATTCAGTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTACGCTGTCGGCCGGGATTTCCTGCAACTGCGCCAgg      >  2:5294192/1‑151 (MQ=255)
                                                                                                                                                   cT‑CC‑GTT‑GCATAGGAGC‑GGATCGGCGCGCCGACTCACTG‑CCA‑G‑C‑GG‑AAATTCAGTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTACGCTGTCGGCCGGGATTTCCTGCAACTGCGCCAgg      >  2:11007166/1‑151 (MQ=255)
                                                                                                                                                   cT‑CC‑GTT‑GCATAGGAGC‑GGATCGGCGCGCCGACTCACTG‑CCA‑G‑C‑GG‑AAATTCAGTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTACGCTGTCGGCCGGGATTTCCTGCAACTGCGCCAgg      >  1:7596523/1‑151 (MQ=255)
                                                                                                                                                    t‑CC‑GTT‑GCATAGGAGC‑GGATCGGCGCGCCGACTCACTG‑CCA‑G‑C‑GG‑AAATTCAGTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTACGCTGTCGGCCGGGATTTCCTGCAACTGCGCCAGGc     >  1:5095555/1‑151 (MQ=255)
                                                                                                                                                      cc‑GTT‑GCATAGGAGC‑GGATCGGCGCGCCGACTCACTG‑CCA‑G‑C‑GG‑AAATTCAGTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTACGCTGTCGGCCGGGATTTCCTGCAACTGCGCCAGGcc    >  2:3404045/1‑151 (MQ=255)
                                                                                                                                                       c‑GTT‑GCATAGGAGC‑GGATCGGCGCGCCGACTCACTG‑CCA‑G‑C‑GG‑AAATTCAGTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTACGCTGTCGGCCGGGATTTCCTGCAACTGCGCCAGGccc   >  1:3595771/1‑151 (MQ=255)
                                                                                                                                                         gTT‑GCATAGGAGC‑GGATCGGCGCGCCGACTCACTG‑CCA‑G‑C‑GG‑AAATTCAGTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCCTCCCGCGGTACGCTGTCGGCCGGGATTTCCTGCCACTGCGCCAGGCCCa  >  2:9816056/1‑151 (MQ=255)
                                                                                                                                                         gTT‑GCATAGGAGC‑GGATCGGCGCGCCGACTCACTG‑CCA‑G‑C‑GG‑AAATTCAGTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTACGCTGTCGGCCGGGATTTCCTGCAACTGCGCCAGGCCCa  >  2:11027124/1‑151 (MQ=255)
                                                                                                                                                         gTT‑GCATAGGAGC‑GGATCGGCGCGCCGACTCACTG‑CCA‑G‑C‑GG‑AAATTCAGTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTACGCTGTCGGCCGGGATTTCCTGCAACTGCGCCAGGCCCa  >  1:5791604/1‑151 (MQ=255)
                                                                                                                                                          |                                                                                                                                                            
AAACGCCGCAGGAGTCACCGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGC‑GCC‑GGCCCGCT‑CC‑GTT‑GCATAGGAGC‑GGATCGGCGCGCCGACTCACTG‑CCA‑G‑C‑GG‑AAATTCAGTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTACGCTGTCGGCCGGGATTTCCTGCAACTGCGCCAGGCCCA  >  NC_008463/4616304‑4616603

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑