Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084635,248,9500TG24.2% 898.0 / 16.0 364L666R (CTG→CGG) tpsA4two‑partner secretion system exoprotein TpsA4
Reads supporting (aligned to +/- strand):  ref base T (60/191);  new base G (78/2);  total (171/193)
Fisher's exact test for biased strand distribution p-value = 8.52e-35
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 372 total aligned reads displayed.

GGAGCAGCGAGAATGTCGGTTCCGGCCTGGCGAATCCAAGCAGCGCGGGAAAAGGTCCGACGCTGCATGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCTGTCC‑TGGGACAGCGG‑CAACGGCCTGACGCTAACCGCCGAGCGCGGGGACCTCAGGATCAATGGCGCGCTGACGGCCCAGGGGGAAAACGCCAGCCTTACTCTCAATGCCGGGCAGCGCCCTCTCCGTATCGACGACAGCCTCTCTCTCAC  >  NC_008463/5248800‑5249100
                                                                                                                                                      |                                                                                                                                                        
ggAGCAGCGAGAATGTCGGTTCCGGCCTGGCGAATCCAAGCAGCGCGGGAAAAGGTCCGACGCTGCATGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCt                                                                                                                                                          <  2:8022589/151‑1 (MQ=255)
ggAGCAGCGAGAATGTCGGTTCCGGCCTGGCGAATCCAAGCAGCGCGGGAAAAGGTCCGACGCTGCATGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCt                                                                                                                                                          >  2:6089115/1‑151 (MQ=255)
 gAGCAGCGAGAATGTCGGTTCCGGCCTGGCGAATCCAAGCAGCGCGGGAAAAGGTCCGACGCTGCATGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCTg                                                                                                                                                         >  1:8182066/1‑151 (MQ=255)
  agcagcGCGCATGACGGTTCCGGCCGGGCGCATCCACGCCGCGCGGGAAAACGGCCGCCGCTGCATGCCGATACCCTGGCCCGCAACCTGTCCACAAGCCACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCTGt                                                                                                                                                        <  2:9688578/151‑1 (MQ=255)
  agcagcGAGAATGTCGGTTCCGGCCTGGCGAATCCAAGCAGCGCGGGAAAAGGTCCGACGCTGCATGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGgtgt                                                                                                                                                        <  1:3477137/151‑1 (MQ=255)
    cagcGAGAATGTCGGTTCCGGCCTGGCGAATCCAAGCAGCGCGGGAAAAGGTCCGACGCTGCATGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGGCGCGGTcc                                                                                                                                                      >  1:10659037/1‑151 (MQ=255)
    cagcGAGAATGTCGGTTCCGGCCTGGCGAATCCAAGCAGCGCGGGAAAAGGTCCGACGCTGCATGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGGCCCGGTcc                                                                                                                                                      >  1:8616787/1‑151 (MQ=255)
     agcGAGAATGTCGGTTCCGGCCTGGCGAATCCAAGCAGCGCGGGAAAAGGTCCGACGCTGCATGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCGGTAC‑t                                                                                                                                                    >  1:10959919/1‑151 (MQ=255)
       cGAGAATGTCGGTTCCGGCCTGGCGAATCCAAGCAGCGCGGGAAAAGGTCCGACGCTGCATGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCTGTCC‑Tgg                                                                                                                                                  <  1:5023375/151‑1 (MQ=255)
          gaATGTCGGTTCCGGCCTGGCGAATCCAAGCAGCGCGGGAAAAGGTCCGACGCTGCATGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCAGTAC‑AGGGAc                                                                                                                                               >  2:9136732/1‑151 (MQ=255)
                 ggTTCCGGCCTGGCGAATCCAAGCAGCGCGGGAAAAGGTCCGACGCTGCATGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGGCGCGGTCC‑GGGGGCAGCGG‑cc                                                                                                                                       >  1:4171170/1‑150 (MQ=255)
                     ccGGCCTGGCGAATCCAAGCAGCGCGGGAAAAGGTCCGACGCTGCATGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCTGGCC‑TGGGACACCGG‑CCACgg                                                                                                                                   >  1:5746233/1‑151 (MQ=255)
                        gCCTGGCGAATCCAAGCAGCGCGGGAAACGGTCCGACGCGGCTTGCCGCTACCCTGGCCCGCAACCTTTCCACCAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCTGTCC‑TGGGACAGCGG‑CAACGGCCt                                                                                                                                <  2:490937/151‑1 (MQ=255)
                         ccTGGCGAATCCAAGCAGCGCGGGAAAAGGTCCGACGCTGCATGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGGCGCTGTCC‑GGGGGCAGCGG‑CAACGGCCTg                                                                                                                               >  1:6389800/1‑151 (MQ=255)
                         ccTGGCGAATCCAAGCAGCGCGGGAAAAGGTCCGACGCTGCATGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCTGTCC‑TGGGACAGCGG‑CAACGGCCTg                                                                                                                               <  1:4573450/151‑1 (MQ=255)
                         ccTGGCGAATCCAAGCAGCGCGGGAAAAGGTCCGACGCTGCATGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCGGTGC‑GGGGGCAGCGG‑CCACGGccag                                                                                                                               >  1:10993250/1‑149 (MQ=255)
                         ccTGGCGAATCCAAGCAGCGCGGGAAAAGGTCCGACGCTGCATGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCCCAGTGC‑AGGGGCAGCGG‑CCACGGccgg                                                                                                                               >  1:782635/1‑149 (MQ=255)
                          cTGGCGAATCCAAGCAGCGCGGGAAAAGGTCCGACGCTGCATGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGGCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCTGGCC‑TGGGACAGCGG‑CCACGGCCTGa                                                                                                                              >  2:9928396/1‑151 (MQ=255)
                           tGGCGAATCCAAGCAGCGCGGGAAAAGGTCCGACGCTGCATGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCTGGGCAACGCGCATGTCGGCGCGCCGCTGTCC‑TGGGACAGCGG‑CAACGGCCTGAc                                                                                                                             <  1:10295052/151‑1 (MQ=255)
                           tGGCGAATCCAAGCAGCGCGGGAAAAGGTCCGACGCTGCATGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCGGTGC‑GGGGGCAGCGGCCAACGGcgggg                                                                                                                              >  1:8900840/1‑147 (MQ=255)
                            ggCGAATCCAAGCAGCGCGGGAAAAGGTCCGACGCTGCATGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGGCGCGGTGC‑TGGGGCAGCGG‑CAACGGCCGGACg                                                                                                                            >  2:9323512/1‑151 (MQ=255)
                              cGAATCCAAGCAGCGCGGGAAAAGGTCCGACGCTGCATGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCTGTCC‑TGGGACAGCGG‑CAACGGCCTGACGCt                                                                                                                          <  1:136016/151‑1 (MQ=255)
                                  tCCAAGCAGCGCGGGAAAAGGTCCGACGCTGCATGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCTGTCC‑TGGGACAGCGG‑CAACGGCCTGACGCTAAcc                                                                                                                      <  1:6538242/151‑1 (MQ=255)
                                         agcGCGGGAAAAGGTCCGACGCTGCATGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCTGTCC‑TGGGACAGCGG‑CAACGGCCTGACGCTAACCGCCGAgc                                                                                                               <  1:3020728/151‑1 (MQ=255)
                                         agcGCGGGAAAAGGTCCGACGCTGCATGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCGGTCC‑TGGGACAGCGG‑CCACGGCCGGTAGCTAAACGCCGAgc                                                                                                               >  2:11240172/1‑151 (MQ=255)
                                              gggAAAAGGTCCGACGCTGCATGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGGCGCGGGCC‑TGGGGCCGCGG‑CCACGGCCGGTCCCGCACCGCCCCGCGCggg                                                                                                          >  1:9755571/1‑151 (MQ=255)
                                              gggAAAAGGTCCGACGCTGCATGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCTGTCC‑TGGGACAGCGG‑CAACGGCCTGACGCTAACCGCCGAGCGCggg                                                                                                          <  1:6677586/151‑1 (MQ=255)
                                              gggAAAAGGTCCGACGCTGCATGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCTGTCC‑TGGGACAGCGG‑CAACGGCCTGACGCTAACCGCCGAGCGCggg                                                                                                          <  1:730422/151‑1 (MQ=255)
                                              gggAAAAGGTCCGACGCTGCATGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCTGTAC‑GGGGACAGCGG‑CAACGGCCTGACGCAAACCGCCCAGCGCggg                                                                                                          >  2:832321/1‑151 (MQ=255)
                                               ggAAAAGGTCCGACGCTGCATGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCTGTCC‑TGGGACAGCGG‑CAACGGCCTGACGCTAACCGCCGAGCGCgggg                                                                                                         <  1:5253034/151‑1 (MQ=255)
                                                 aaaaGGTCCGACGCTGCATGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGGCGCGGTGC‑AGGGGCCGCGG‑GCCCCGCCGGACGCGAAACCCCCCGCGCgggggc                                                                                                       >  1:808485/1‑149 (MQ=255)
                                                 aaaaGGTCCGACGCTGCATGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCCCTGTAC‑AGGGACAGCGG‑CAACCGCCGGTCGCGAACCCCCCAGCGCggggcc                                                                                                       >  2:5907559/1‑149 (MQ=255)
                                                       tCCGACGCTGCATGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGGCGCGGTGC‑GGGGTCAGCGG‑CAACGGCCGGACGCGAAACGCCCCGCGCGGGGGccgccgg                                                                                                 >  2:4746045/1‑146 (MQ=255)
                                                       tCCGACGCTGCATGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGGCGCGGTGC‑GGGGGCAGCCG‑CAACCGCCCGACGCGAAACCCCCAGCGCGGGGGCCCCAgg                                                                                                 >  1:1682161/1‑151 (MQ=255)
                                                       tCCGACGCTGCATGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCTGTCC‑CGGGACAGCGG‑CCACGGCCGGACGCTAACCCCCGCGCGCCGGGGCCCCAgg                                                                                                 >  2:10404105/1‑151 (MQ=255)
                                                        ccGACGCTGCATGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCCCAGTAC‑GGGGACCGCGG‑CAAACGCCATACGCAAAACCCCCAGCGCCGGGACCCCAGGa                                                                                                >  2:3507439/1‑151 (MQ=255)
                                                           aCGCTGCATGCCGAGACCCTGGCCCGCAACCGTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGAGCGCCGCTGTCC‑TGGGACAGCGG‑CAACGGCCTGACGCTAACCGCCGAGCGCGGGGACCTCAGGATCa                                                                                             <  2:8682651/151‑1 (MQ=255)
                                                             gCTGCATGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGGCCCTGTCC‑TGGGACAGCGG‑CAACCGCCAGACGCGAACCGCCGAGCGCGGGGGCCCCCGGATCaaa                                                                                           >  2:7667512/1‑150 (MQ=255)
                                                             gCTGCATGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCAAGTCGGCGCGCCCCTGTCC‑AGGGACACCGG‑CAACAGCCAGACCCACAACGCCGAGCGCGGGGACCTCAGGAtacat                                                                                           >  2:6971681/1‑147 (MQ=255)
                                                                   tGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGGCCCTGTCA‑GTGGGCAGCGG‑GAACCCCCTGACCCGAACCCCCCAGCCCCGGGGCCGCAAGAACAAAGgcgcg                                                                                     >  1:2547290/1‑151 (MQ=255)
                                                                   tGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCTGTCC‑TGGGACAGCGG‑CAACGGCCTGACGCTAACCGCCGAGCGCGGGGACCTCAGGATCAATGgcgcg                                                                                     <  2:5100212/151‑1 (MQ=255)
                                                                        aTACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGGCCCGGTCC‑AGGGACCGCGG‑CAACCGCCAGACGCAAACCCCCCCGCGCGGGGCCCCAACGATCAAATGGGCGCGGAc                                                                                >  1:9875831/1‑151 (MQ=255)
                                                                        aTACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCAGTCC‑TGGGACCGCGG‑CCACGGCCAGAAGCAAACCCCCGAGCGCGGGGCCCACAGGATCCAAGGCGCGCGGAc                                                                                >  2:1454781/1‑151 (MQ=255)
                                                                         tACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGGCGCTGTGC‑TGGGACAGCGG‑GAACCGCCTGTAGCTAACCGCCGAGGGCGGGGGGCTCAGGGTCTATGGCGGGGTGACg                                                                               >  2:7458033/1‑151 (MQ=255)
                                                                          aCCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCTGTCC‑TGGGACAGCGG‑CAACGGCCTGACGCTAACCGCCGAGCGCGGGGACCTCAGGATCAATGGCGCGCTGACgg                                                                              <  1:5651846/151‑1 (MQ=255)
                                                                             cTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCTGTCC‑TGGGACAGCGG‑CAACGGCCTGACGCTAACCGCCGAGCGCGGGGACCTCAGGATCAATGGCGCGCGGACGGccc                                                                           <  2:1928447/151‑1 (MQ=255)
                                                                              tGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCCTGTCGGCGCGCCCCGGGCC‑GGGGGCAGCGG‑CCCCGGCCTGACGCGAAACCCCCCGCGCGGGGCCCACAGGCTCCAAGGCGCGCGGCCGGCCCa                                                                          >  2:10887889/1‑151 (MQ=255)
                                                                              tGGCCCGCAACCTGTCCACAAGCCACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCTGTCC‑TGGGACAGCGG‑CAACGGCCTGACGCTAACCGCCGAGCGCGGGGACCTCAGGATCAATGGCGCGCTGACGGCCCa                                                                          <  1:2073258/151‑1 (MQ=255)
                                                                               ggCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCTGTCC‑TGGGACAGCGG‑CAACGGCCTGACGCTAACCGCCGAGCGCGGGGACCTCAGGATCAATGGCGCGCTGACGGCCCAg                                                                         <  2:4023584/151‑1 (MQ=255)
                                                                                gCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCTGTCC‑TGGGACAGCGG‑CAACGGCCTGACGCTAACCGCCGAGCGCGGGGACCTCAGGATCAATGGCGCGCTGACGGCCCAgg                                                                        <  2:7194784/151‑1 (MQ=255)
                                                                                gCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCTGTCC‑TGGGACAGCGG‑CAACGGCCTGACGCTAACCGCCGAGCGCGGGGACCTCAGGATCAATGGCGCGCTGACGGCCCAgg                                                                        <  1:6505797/151‑1 (MQ=255)
                                                                                 cccGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCGGTGC‑GGGGGCAGCGG‑CCAACGCCTGACGCAAACCCCCCAGCGCGGGGCCCGGAGGATCAAAGGCGCGCGGACGGCCCCggg                                                                       >  2:1222773/1‑151 (MQ=255)
                                                                                  ccGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCTGTCC‑TGGGACAGCGG‑CAACGGCCTGACGCTAACCGCCGAGCGCGGGGACCTCAGGACCAATGGCGCGCTGACGGCCCAgggg                                                                      <  1:7233912/151‑1 (MQ=255)
                                                                                  ccGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCGGTCC‑AGGGGCAGCGG‑CAACGGCCGGACGCGAACCGCCCAGCGCGGGGAGCTCAGGGTCAATGGCGCGCGGTCGGCCCCgggg                                                                      >  2:5563938/1‑151 (MQ=255)
                                                                                  ccGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCCCGGTCC‑TGGGACAGCGG‑CAACGGCCGGACGCTAAACGCCCAGCGCGGGGACCGCAGGATCAAATGCGCGCTGACGGCCCCgggg                                                                      >  2:10216261/1‑151 (MQ=255)
                                                                                   cGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCAGTCC‑TGGGACAGCGG‑CCACGGCCGGGAGCGAACCCCCGAGCGCGGGGCCCGCCGGATCAATGGCGGGGGGTCGGCCCAggggg                                                                     >  2:9407725/1‑151 (MQ=255)
                                                                                     cAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCCCTGTCC‑GGGGGCACCGG‑CAACGGCCGGAAGCGAACCCCCCGGCGCGGGGGCCGCAGGGTAAAATGCGCGCGGGAGGCCCCCGGGGaa                                                                   >  2:736264/1‑151 (MQ=255)
                                                                                      acccTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCCCGGTAC‑TGGGTCAGCGGCCAACGG‑CAGACGCGAACCCCCCAGCCCGGGGGGCGCCCGATAAAAGGCGCGCGGACGGCCCCggggggca                                                                  >  1:9228289/3‑148 (MQ=255)
                                                                                        ccTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCTGTGA‑TGGGTACGCGG‑CCACCGCCGGGCGCTAACCGCCGAGCGCGGGGGGCTCAGGGTGAAAGGGGCGCGGGCGGCCCCGGGGGagaaa                                                                >  2:177841/1‑150 (MQ=255)
                                                                                        ccTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCTGTCC‑TGGGACAGCGG‑CAACGGCCTGACGCTAACCGCCGAGCGCGGGGACCTCAGGATCAATGGCGCGCTGACGGCCCAGGGGGAAAAc                                                                <  1:7420457/151‑1 (MQ=255)
                                                                                        ccTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCTGTCC‑TGGGACAGCGG‑CAACGGCCTGACGCTAACCGCCGAGCGCGGGGACCTCAGGATCAATGGCGCGCTGACGGCCCAGGGGGAAAAc                                                                <  1:441935/151‑1 (MQ=255)
                                                                                         cTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGGCGCAGTGC‑GACGGCCGCGG‑CAACCGCCTCGAGCAAAACGCCGAGGGCGGCGCCCGGAGGGGCCAAGGCGCGCGGGCCGCCCCgggggggctgg                                                               >  2:2985522/1‑145 (MQ=255)
                                                                                          tttCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCTGTCC‑TGGGACAGCGG‑CAACGGCCTGACGCTAACCGCCGAGCGCGGGGACCTCAGGATCAATGGCGCGCTGACGGCCCAGGGGGAAAACGc                                                              <  2:777270/151‑1 (MQ=255)
                                                                                           ttCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCTGTCC‑TGGGACAGCGG‑CAACGGCCTGACGCTAACCGCCGAGCGCGGGGACCTCAGGATCAATGGCGCGCTGACGGCCCAGGGGGAAAACGcc                                                             <  1:1095069/151‑1 (MQ=255)
                                                                                            tCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCTGTCC‑TGGGACAGCGG‑CAACGGCCTGACGCTAACCGCCGAGCGCGGGGACCTCAGGATCAATGGCGCGCGGACGGCCCAGGGGGAAAACGCCa                                                            <  2:10589755/151‑1 (MQ=255)
                                                                                             ccACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCTTGTCGGCGCGCCGCTGTCC‑TGGGTCAGCGG‑CAACGGCCTGACGCTAACCGCCGAGCGCGGGGACCTCAGGATCAATGGCGCGCTGACGGCCCAGGGGGAAAACGCCAg                                                           <  2:5174405/151‑1 (MQ=255)
                                                                                                  aGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGGCGCTGGGC‑GGGGGCCGCGG‑GAAAGGCCAGACGCGAACCCCCGGGCGCGGGGGCCTCATGGTACATGGCGCGCGGACGGCCCAGGGGGCAAACGCCAGCCGTa                                                      >  2:819446/1‑151 (MQ=255)
                                                                                                   gCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCTGTCC‑TGGGACAGCGG‑CAACGGCCTGACGCTAACCGCCGAGCGCGGGGACCTCAGGATCAATGGCGCGCTGACGGCCCAGGGGGAAAACGCCAGCCTTAc                                                     <  1:6217924/151‑1 (MQ=255)
                                                                                                    cAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCTGTCC‑TGGGACAGCGG‑CAACGGCCTGACGCTAACCGCCGAGCGCGGGGACCTCAGGATCAATGGCGCGCTGACGGCCCAGGGGGAAAACGCCAGCCTTAct                                                    <  1:1038027/151‑1 (MQ=255)
                                                                                                    cAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGAGGCGCGGTCC‑GGGGACAGCCG‑CAACCGCCGGACGCAAACCGCCCATCGCGGGGCGCGCAAGATAACAAGCGCGCAGTCGGCCCCGGGGGGAAAAGCCAcccagaaa                                                    >  2:9453862/1‑146 (MQ=255)
                                                                                                     aaCGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCGGTCC‑GGGGCCCGCGG‑CAACCGCCAGACGCGAAACCCCGCGCGCGGGGGCCGCAGGATCCATGGCGCGCGGACGGCCCACGGGGGAAACGCCAGCCATaaac                                                   >  2:10611919/1‑148 (MQ=255)
                                                                                                      aCGTCGACGGGGACGCCCCCCGGGGCAACGCGCCTGGCGGCGCGCCGCTGTCC‑GGGGACAGCGG‑CAACGGCCTGCCGCTAACCGCCGCGCGCGGGGACCACAGGATCAATGGCGCGCTGACGGCCCAGGGGGAAAACGCCAGCCTTCctct                                                  <  2:2607255/151‑1 (MQ=255)
                                                                                                      aCGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCTGTCC‑TGGGACAGCGG‑CAACGGCCTGACGCTAACCGCCGAGCGCGGGGACCTCAGGATCAATGGCGCGCTGACGGCCCAGGGGGAAAACGCCAGCCTTActct                                                  <  2:1426102/151‑1 (MQ=255)
                                                                                                       cGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCCCAGGGC‑AGGGACAGCGG‑CAAACGCCAGAACCAAACCGCCCAGCGCCGGGCCCACAGGATCAATGGCGCGCTGAAGGGCCCCGGGGTAAACCCCCGCCGTaaacaa                                                 >  2:4486727/1‑146 (MQ=255)
                                                                                                           gAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCGGTCC‑AGGGACAGCCG‑CAACCGCCGGACCAAAACCGCCGAGCGCCGGGCCCGCCGGTTAAAAGGCGCGCGGACGGCCCAGGGGGCAAACGCCCCCCGTAATCTCAATg                                             >  2:6529185/1‑151 (MQ=255)
                                                                                                            aaGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCTGTCC‑TGGGACAGCGG‑CAACGGCCTGACGCTAACCGCCGAGCGCGGGGACCTCAGGATCAATGGCGCGCTGACGGCCCAGGGGGAAAACGCCAGCCTTACTCTCAATGc                                            <  2:525108/151‑1 (MQ=255)
                                                                                                            aaGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCTGTCC‑TGGGACAGCGG‑CAACGGCCTGACGCTAACCGCCGAGCGCGGGGACCTCAGGATCAATGGCGCGCTGACGGCCCAGGGGGAAAACGCCAGCCTTACTCTCAATGc                                            <  1:3845814/151‑1 (MQ=255)
                                                                                                                ggTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCTGTCC‑TGGGACAGCGG‑CAACGGCCTGACGCTAACCGCCGAGCGCGGGGACCTCAGGATCAATGGCGCGCTGACGGCCCAGGGGGAAAACGCCAGCCTTACTCTCAATGCCggg                                        <  2:11159099/151‑1 (MQ=255)
                                                                                                                 gTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCTGTCC‑TGGGACAGCGG‑CAACGGCCTGACGCTAACCGCCGAGCGCGGGGACCTCAGGATCAATGGCGCGCTGACGGACCAGGGGGAAAACGCCAGCCTTACTCTCACTGCCGGGc                                       <  2:3922709/151‑1 (MQ=255)
                                                                                                                 gTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCCCGGGCCATGGCAC‑CCGG‑CAACCGCCGGACCCGAACCGCCCAGCGCGGGGGCCTCAGGATGAAGTGCGCCCTGCCGGCCCACGGGGAACTCGCCAGCCGGACGGTCAAAGCCgggg                                       >  2:5435853/1‑150 (MQ=255)
                                                                                                                  tCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCTGTCC‑TGGGACAGCGG‑CAACGGCCTGACGCTAACCGCCGAGCGCGGGGACCTCAGGATCAATGGCGCGCTGACGGCCCAGGGGGAAAACGCCAGCCTTACTCTCAATGCCGGGCa                                      <  1:3125341/151‑1 (MQ=255)
                                                                                                                     ccACCCGGGGCAACGCGCATGTCGGCGCGCCGCTGTCC‑TGGGACAGCGG‑CAACGGCCTGACGCTAACCGCCGAGCGCGGGGACCTCAGGATCAATGGCGCGCTGACGGCCCAGGGGGAAAACGCCAGCCTTACTCTCAATGCCGGGCAgcg                                   <  2:8848294/151‑1 (MQ=255)
                                                                                                                       aCCCGGGGCAACGCGCATGTCGGCGCGCCGCGGTGC‑GGGGGCCGGGG‑GGACCGGCAGTCGGGAACCGCCCTGCGCGGGGGCCGCGGGCTGAATGGCGGGCTGTGGGGCCCGGGGGGTATCACCCGCCTGGCGCTCCATGACGGgcggcggc                                 >  2:3731613/1‑149 (MQ=255)
                                                                                                                       aCCCGGGGCAACGCGCATGTCGGCGCGCCGCAGGGC‑GGGGACACCCG‑CACACGCCGGCCGCGAACCGCCCAGCGCGGGGGCCCCCGGATCAAAGGCGCCCTGAAGGCCCCCGGGGAAAACGCCAGCCAAAGACTAAATGCCGGGgcgcgcg                                 >  2:6650876/1‑150 (MQ=255)
                                                                                                                       aCCCGGGGCAACGCGCATGTCGGCGCGCCGCAGGAC‑TGGGACCGCCG‑CAAAGGCCTGACGCTAACCGCCGAGCGCCGGGACCGCTGGATAACTGGCGCGCTGTCGGCCCAGGGGGATAACCCCAGCCATAAACACGAAGCCGGGCAGCGcc                                 >  2:6474625/1‑151 (MQ=255)
                                                                                                                        cccGGGGCAACGCGCATGTCGGCGCGCCGCTGTCC‑TGGGACAGCGG‑CAACGGCCTGACGCTAACCGCCGAGCGCGGGGACCTCAGGATCAATGGCGCGCTGACGGCCCAGGGGGAAAACGCCAGCCTTACTCTCAATGCCGGGCAGCGccc                                <  1:7777861/151‑1 (MQ=255)
                                                                                                                             ggCAACGCGCATGTCGGCGCGCCGCTGTCC‑TGGGACAGCGG‑CAACGGCCTGACGCTAACCGCCGAGCGCGGGGACCTCAGGATCAATGGCGCGCTGACGGCCCAGGGGGAAAACGCCAGCCTTACTCTCAATGCCGGGCAGCGCCCTCTcc                           <  2:10960472/151‑1 (MQ=255)
                                                                                                                              gCAACGCGCATGTCGGCGCGCCGCTGTCC‑TGGGACAGCGG‑CAACGGCCTGACGCTAACCGCCGAGCGCGGGGACCTCAGGATCAATGGCGCGCTGACGGCCCAGGGGGAAAACGCCAGCCTTACTCTCAATGCCGGGCAGCGCCCTCTCCg                          <  2:3416257/151‑1 (MQ=255)
                                                                                                                               cAACGCGCATGTCGGCGCGCCGCTGTCC‑TGGGACAGCGG‑CAACGGCCTGACGCTAACCGCCGAGCGCGGGGACCTCAGGATCAATGGCGCGCTGACGGCCCAGGGGGAAAACGCCAGCCTTACTCTCAATGCCGGGCAGCGCCCTCTCCGt                         <  1:155235/151‑1 (MQ=255)
                                                                                                                                aaCGCGCATGTCGGCGCGCCGCTGTCC‑TGGGACAGCGG‑CAACGGCCTGACGCTAACCGCCGAGCGCGGGGACCTCAGGATCAATGGCGCGCTGACGGCCCAGGGGGAAAACGCCAGCCTTACTCTCAATGCCGGGCAGCGCCCTCTCCGTa                        <  2:5344305/151‑1 (MQ=255)
                                                                                                                                 aCGCGCATGTCGGCGCGCCGCTGTCC‑TGGGACAGCGG‑CAACGGCCTGACGCTAACCGCCGAGCGCGGGGACCTCAGGATCAATGGCGCGCTGACGGCCCAGGGGGAAAACGCCAGCCTTACTCTCAATGCCGGGCAGCGCCCTCTCCGTAt                       <  1:2799178/151‑1 (MQ=255)
                                                                                                                                        tGTCGGCGCGCCGCTGTCC‑TGGGACAGCGG‑CAACGGCCTGACGCTAACCGCCGAGCGCGGGGACCTCAGGATCAATGGCGCGCTGACGGCCCAGGGGGAAAACGCCAGCCTTACTCTCAATGCCGGGCAGCGCCCTCTCCGTAACgacgac                <  2:3795297/151‑1 (MQ=255)
                                                                                                                                            ggCGCGCCGCTGTCC‑TGGGACAGCGG‑CAACGGCCTGACGCTAACCGCCGAGCGCGGGGACCTCAGGATCAATGGCGCGCTGACGGCCCAGGGGGAAAACGCCAGCCTTACTCTCAATGCCGGGCAGCGCCCTCTCCGTATCGACGACAGcc            <  1:967060/151‑1 (MQ=255)
                                                                                                                                            ggCGCGCCGCTGTCC‑TGGGACAGCGG‑CAAAGGCCTGACGCTAACCGCCGATCGCGGGGACCTCAGGATAAATGGCGCGCTGACGGCCCAGGGGGAAAACGCCAGCCTTACACTAAATGCCGGGCAGCGCCCTCTCCGTATCGACGACAGcc            >  2:4153249/1‑151 (MQ=255)
                                                                                                                                             gcgcgcCGCTGTCC‑TGGGACAGCGG‑CAACGGCCTGACGCTAACCGCCGAGCGCGGGGACCTCAGGATCAATGGCGCGCTGACGGCCCAGGGGGAAAACGCCAGCCTTACTCTCAATGCCGGGCAGCGCCCTCTCCGTATCGACGACAGCct           <  2:2264292/151‑1 (MQ=255)
                                                                                                                                              cgcgcCGCTGTCC‑TGGGACAGCGG‑CAACGGCCTGACGCTAACCGCCGAGCGCGGGGACCTCAGGATCAATGGCGCGCTGACGGCCCAGGGGGAAAACGCCAGCCTTACTCTCAATGCCGGGCAGCGCCCTCTCCGTATCGACGACAGCctc          <  1:9077401/151‑1 (MQ=255)
                                                                                                                                               gcgcCGCTGTCC‑TGGGACAGCGG‑CAACGGCCTGACGCTAACCGCCGAGCGCGGGGACCTCAGGATCAATGGCGCGCTGACGGCCCAGGGGGAAAACGCCAGCCTTACTCTCACTGCCGGGCAGCGCCCTCTCCGTATCGACGACAGCctct         <  2:6483835/151‑1 (MQ=255)
                                                                                                                                               gcgcCGCTGTCC‑TGGGACAGCGG‑CAACGGCCTGACGCGAACCGCCGAGCGCGGGGACCTCAGGATCAATGGCGCGCTGACGGCCCAGGGGGAAAACGCCAGCCTTACTCTCAATGCCGGGCAGCGCCCTCTCCGTATCGACGACAGCctct         <  2:7301883/151‑1 (MQ=255)
                                                                                                                                                     cTGTCC‑TGGGACAGCGG‑CAACGGCCTGACGCTAACCGCCGAGCGCGGGGACCTCAGGATCAATGGCGCGCTGACGGCCCAGGGGGAAAACGCCAGCCTTACTCTCAATGCCGGGCAGCGCCCTCTCCGTATCGACGACAGCCTCTCTCTCa   >  1:8438485/1‑151 (MQ=255)
                                                                                                                                                      tGTCC‑TGGGACAGCGG‑CAACGGCCTGACGCTAACCGCCGAGCGCGGGGACCTCAGGATCAATGGCGCGCTGACGGCCCAGGGGGAAAACGCCAGCCTTACTCTCAATGCCGGGCAGCGCCCTCTCCGTATCGACGACAGCCTCTCTCTCAc  >  1:7051095/1‑151 (MQ=255)
                                                                                                                                                      |                                                                                                                                                        
GGAGCAGCGAGAATGTCGGTTCCGGCCTGGCGAATCCAAGCAGCGCGGGAAAAGGTCCGACGCTGCATGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCTGTCC‑TGGGACAGCGG‑CAACGGCCTGACGCTAACCGCCGAGCGCGGGGACCTCAGGATCAATGGCGCGCTGACGGCCCAGGGGGAAAACGCCAGCCTTACTCTCAATGCCGGGCAGCGCCCTCTCCGTATCGACGACAGCCTCTCTCTCAC  >  NC_008463/5248800‑5249100

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑