Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 2,955,357 A→G L314L (TTG→CTG)  PA14_RS13700 ← PepSY domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084632,955,3570AG100.0% 1752.6 / NA 444L314L (TTG→CTG) PA14_RS13700PepSY domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (199/245);  total (199/245)

Only 100 of 444 total aligned reads displayed.

CAGATCAGCAGGCCGCTGAGCACCATCATGCTCAGCAGCAGGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAAGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCAGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAAGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCC  >  NC_008463/2955209‑2955504
                                                                                                                                                    |                                                                                                                                                   
cAGATCAGCAGGCCGCTGAGCACCATCATGCTCAGCAGCAGGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGc                                                                                                                                                   <  1:8526716/151‑1 (MQ=255)
  gATCAGCAGGCCGCTGAGCACCATCATGCTCAGCAGCAGGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCgg                                                                                                                                                 <  2:1685247/151‑1 (MQ=255)
   aTCAGCAGGCCGCTGAGCACCATCATGCTCCGCAGCAGGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAACTCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCggg                                                                                                                                                >  2:9082461/1‑151 (MQ=255)
    tCAGCAGGCCGCTGAGCACCATCATGCTCAGCAGCAGGCCGAAGACGAACCAGATCAGCTTGACCCAGAGACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGggt                                                                                                                                               <  2:8506428/151‑1 (MQ=255)
     cagcagGCCGCTGAGCACCATCATGCTCAGCAGCAGGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGggtg                                                                                                                                              >  1:10442660/1‑151 (MQ=255)
      agcagGCCGCTGAGCACCATCATGCTCAGCAGCAGGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGggtgg                                                                                                                                             <  2:8790359/151‑1 (MQ=255)
        cagGCCGCTGAGCACCATCATGCTCAGCAGCAGGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGtt                                                                                                                                           <  2:4809383/151‑1 (MQ=255)
          ggCCGCTGAGCACCATCATGCTCAGCAGCAGGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTc                                                                                                                                         >  1:8247570/1‑151 (MQ=255)
           gCCGCTGAGCACCATCATGCTCAGCAGCAGGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAACTCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCg                                                                                                                                        <  2:1834911/151‑1 (MQ=255)
            ccGCTGAGCACCATCATGCTCAGCAGCAGGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGa                                                                                                                                       >  1:9114020/1‑151 (MQ=255)
               cTGAGCACCATCATGCTCAGCAGCAGGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCt                                                                                                                                    <  1:7349723/151‑1 (MQ=255)
                tGAGCACCATCATGCTCAGCAGCAGGCCGAAGACGAACCAGATCAGCTTGACCCAGATCCCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCtt                                                                                                                                   <  2:8136174/151‑1 (MQ=255)
                tGAGCACCATCATGCTCAGCAGCAGGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCtt                                                                                                                                   >  1:886599/1‑151 (MQ=255)
                tGAGCACCATCATGCTCAGCAGCAGGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCtt                                                                                                                                   <  2:5276200/151‑1 (MQ=255)
                tGAGCACCATCATGCTCAGCAGCAGGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCtt                                                                                                                                   >  2:7836862/1‑151 (MQ=255)
                  aGCACCATCCTGCTCAGCAGCAGGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGc                                                                                                                                 <  1:2155610/151‑1 (MQ=255)
                    cACCATCATGCTCAGCAGCAGGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGACCTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCg                                                                                                                               >  2:3692346/1‑151 (MQ=255)
                        atcatGCTCAGCAGCAGGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGtat                                                                                                                           <  1:960454/151‑1 (MQ=255)
                        atcatGCTCAGCAGCAGGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGtat                                                                                                                           <  2:1532295/151‑1 (MQ=255)
                          catGCTCAGCAGCAGGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATaa                                                                                                                         >  2:2671861/1‑151 (MQ=255)
                            tGCTCAGCAGCAGGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAgg                                                                                                                       >  1:6897956/1‑151 (MQ=255)
                             gCTCAGCAGCAGGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAggg                                                                                                                      <  2:9379009/151‑1 (MQ=255)
                                cagcagcagGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTc                                                                                                                   >  1:10587799/1‑151 (MQ=255)
                                 agcagcagGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCa                                                                                                                  >  2:704735/1‑151 (MQ=255)
                                    agcagGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTGTCGATCTTGCCGGTATAAGGGTTCAgcg                                                                                                               >  2:2921250/1‑151 (MQ=255)
                                      cagGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGcgcg                                                                                                             <  1:8719124/151‑1 (MQ=255)
                                        ggCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGc                                                                                                           <  1:6964334/151‑1 (MQ=255)
                                         gCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGcc                                                                                                          <  2:3385960/151‑1 (MQ=255)
                                              aGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTg                                                                                                     <  1:5447073/151‑1 (MQ=255)
                                                   aaCCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACa                                                                                                <  2:5778542/151‑1 (MQ=255)
                                                      cAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGa                                                                                             <  2:3597498/151‑1 (MQ=255)
                                                        gtaatgCTTGACCCAGATCCCGCCGAAATCTGCGGAGTGCAGGGGACGCATGGAGTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGAACTTGCCGGTTTAAGGGTTCAGCGCGGCCGACTGGAACATGAGc                                                                                           <  2:8369945/146‑1 (MQ=255)
                                                         aTCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCg                                                                                          >  1:834638/1‑151 (MQ=255)
                                                         aTCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCg                                                                                          >  2:9790053/1‑151 (MQ=255)
                                                           cAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGAGTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGa                                                                                        <  2:3208852/151‑1 (MQ=255)
                                                            aGCTTGACCCAGATACCGCCGACCTCGCCGGTGTGCAGGGGACGCATGGCTTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGAt                                                                                       <  2:4047171/151‑1 (MQ=255)
                                                             gCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATa                                                                                      <  1:5231849/151‑1 (MQ=255)
                                                               ttGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATAcc                                                                                    >  2:9005992/1‑151 (MQ=255)
                                                                 gACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAg                                                                                  <  2:5515504/151‑1 (MQ=255)
                                                                  aCCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGc                                                                                 <  1:1663312/151‑1 (MQ=255)
                                                                    ccAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCcg                                                                               >  1:45454/1‑151 (MQ=255)
                                                                     cAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCcgc                                                                              >  1:11209637/1‑151 (MQ=255)
                                                                      aGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCcgcg                                                                             >  1:10018272/1‑151 (MQ=255)
                                                                      aGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCcgcg                                                                             >  1:4788492/1‑151 (MQ=255)
                                                                       gATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGGGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGt                                                                            <  2:4410196/151‑1 (MQ=255)
                                                                        aTACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTc                                                                           <  1:7588678/151‑1 (MQ=255)
                                                                        aTACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTc                                                                           >  1:5546689/1‑151 (MQ=255)
                                                                        aTACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTc                                                                           <  1:3289659/151‑1 (MQ=255)
                                                                         tACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGACCATGAGCGGATACCAGCCGCGTcc                                                                          >  1:10125429/1‑151 (MQ=255)
                                                                         tACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTcc                                                                          >  1:11146762/1‑151 (MQ=255)
                                                                         tACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTcc                                                                          <  1:3244020/151‑1 (MQ=255)
                                                                         tACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTcc                                                                          <  2:10097190/151‑1 (MQ=255)
                                                                               cgAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCAc                                                                    >  2:5913676/1‑151 (MQ=255)
                                                                                gAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACg                                                                   <  1:2060707/151‑1 (MQ=255)
                                                                                gAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACg                                                                   >  1:9921959/1‑151 (MQ=255)
                                                                                 aaaTCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGACCTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGa                                                                  >  2:5436015/1‑151 (MQ=255)
                                                                                 aaaTCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGa                                                                  <  1:10388692/151‑1 (MQ=255)
                                                                                   aTCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATTCCAGCCGCGTCCGGCCACGAAc                                                                <  2:4004411/151‑1 (MQ=255)
                                                                                       ccGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCg                                                            <  1:10941847/151‑1 (MQ=255)
                                                                                        cGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCgg                                                           >  1:6332465/1‑151 (MQ=255)
                                                                                           tgtgCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATATTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCt                                                        <  2:460017/151‑1 (MQ=255)
                                                                                             tgCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGt                                                      <  1:6360741/151‑1 (MQ=255)
                                                                                              gCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTa                                                     <  2:6658318/151‑1 (MQ=255)
                                                                                              gCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTa                                                     <  1:4233468/151‑1 (MQ=255)
                                                                                                aGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAgg                                                   >  2:451946/1‑151 (MQ=255)
                                                                                                 ggggACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGc                                                  <  2:7342157/151‑1 (MQ=255)
                                                                                                   ggACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGt                                                <  2:4299555/151‑1 (MQ=255)
                                                                                                    gACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGtt                                               >  1:7768602/1‑151 (MQ=255)
                                                                                                       gCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGcc                                            >  1:5031617/1‑151 (MQ=255)
                                                                                                        cATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCg                                           >  2:7556252/1‑151 (MQ=255)
                                                                                                        cATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCg                                           >  1:4062022/1‑151 (MQ=255)
                                                                                                        cATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCg                                           <  2:11003918/151‑1 (MQ=255)
                                                                                                          tGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCggg                                         <  1:2394625/151‑1 (MQ=255)
                                                                                                             aTTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCag                                      <  1:9332859/151‑2 (MQ=255)
                                                                                                             aTTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCag                                      >  2:2033347/1‑150 (MQ=255)
                                                                                                                cGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGaa                                   <  1:10765607/151‑1 (MQ=255)
                                                                                                                cGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGaa                                   >  1:6749380/1‑151 (MQ=255)
                                                                                                                 ggTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAac                                  >  2:2591357/1‑151 (MQ=255)
                                                                                                                    gACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACAcc                               <  1:7277010/151‑1 (MQ=255)
                                                                                                                    gACGAACTGCAGGGGGGGACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGTGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACAcc                               >  2:10712040/1‑151 (MQ=255)
                                                                                                                     aCGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACcg                              <  2:5210176/151‑1 (MQ=255)
                                                                                                                     aCGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCGGTAGGCGTTGCCGGGCAGGAACACcg                              >  1:753619/1‑151 (MQ=255)
                                                                                                                       gAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACcgcg                            >  1:28208/1‑151 (MQ=255)
                                                                                                                         aCTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCt                          >  1:2662359/1‑151 (MQ=255)
                                                                                                                         aCTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCt                          <  1:2563398/151‑1 (MQ=255)
                                                                                                                            gCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGc                       <  2:5713266/151‑1 (MQ=255)
                                                                                                                               ggggggTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTc                    <  1:6048581/151‑1 (MQ=255)
                                                                                                                               ggggggTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTc                    <  1:1322952/151‑1 (MQ=255)
                                                                                                                                gggggTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTcc                   <  1:3904908/151‑1 (MQ=255)
                                                                                                                                  gggTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAg                 >  1:7853348/1‑151 (MQ=255)
                                                                                                                                   ggTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAgg                <  1:10324470/151‑1 (MQ=255)
                                                                                                                                    gTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAgcc               <  1:5588977/151‑3 (MQ=255)
                                                                                                                                      aCGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTg             >  2:2192825/1‑151 (MQ=255)
                                                                                                                                        ggTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGTAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTggg           >  1:656119/1‑151 (MQ=255)
                                                                                                                                        ggTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTggg           >  1:4159710/1‑151 (MQ=255)
                                                                                                                                         gTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTgggg          <  1:3527014/151‑1 (MQ=255)
                                                                                                                                           cGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGAt        <  1:292339/151‑1 (MQ=255)
                                                                                                                                               gAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGc    >  2:1145127/1‑151 (MQ=255)
                                                                                                                                               gAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGc    >  1:3612714/1‑151 (MQ=255)
                                                                                                                                               gAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGc    >  1:7071715/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CAGATCAGCAGGCCGCTGAGCACCATCATGCTCAGCAGCAGGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAAGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCAGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAAGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCC  >  NC_008463/2955209‑2955504

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑