Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 3,051,413 T→C K369E (AAG→GAG)  PA14_RS13990 ← SfnB family sulfur acquisition oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084633,051,4130TC100.0% 2051.8 / NA 530K369E (AAG→GAG) PA14_RS13990SfnB family sulfur acquisition oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (271/259);  total (271/259)

Only 100 of 530 total aligned reads displayed.

GGCTCATCGGGCGCTCCTCAGATCCATGAGTGACGCGCCGGACGGGCGCCGTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTTGAGGTTGAACTTGGCGAGCGTGGCGCGGCTGCCGGCCAGCTTGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTCGGCGTCCACCGGCCGCGCGGCCACTTCGTC  >  NC_008463/3051265‑3051560
                                                                                                                                                    |                                                                                                                                                   
 gCTCATCGGGCGCTCCTCAGATCCATGAGTGACGCGCCGGACGGGCGCCGTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGc                                                                                                                                                  <  2:9245318/151‑1 (MQ=255)
   tCATCGGGCGCTCCTCAGATCCATGAGTGACGCGCCGGACGGGCGCCGTTCAGGTAGGAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGa                                                                                                                                                <  2:6021304/151‑1 (MQ=255)
    cATCGGGCGCTCCTCAGATCCATGAGTGACGCGCCGGACGGGCGCCGTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAg                                                                                                                                               >  1:5501144/1‑151 (MQ=255)
    cATCGGGCGCTCCTCAGATCCATGAGTGACGCGCCGGACGGGCGCCGTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAg                                                                                                                                               <  1:8969657/151‑1 (MQ=255)
     aTCGGGCGCTCCTCAGATCCATGAGTGACGCGCCGGACGGGCGCCGTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGc                                                                                                                                              >  2:10334284/1‑151 (MQ=255)
       cGGGCGCTCCTCAGATCCATGAGTGACGCGCCGGACGGGCGCCGTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGt                                                                                                                                            >  1:5241310/1‑151 (MQ=255)
       cGGGCGCTCCTCAGATCCATGAGTGACGCGCCGGACGGGCGCCGTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGt                                                                                                                                            >  1:5959913/1‑151 (MQ=255)
        gggCGCTCCTCAGATCCATGAGTGACGCGCCGGACGGGCGCCGTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTg                                                                                                                                           >  2:8862771/1‑151 (MQ=255)
        gggCGCTCCTCAGATCCATGAGTGACGCGCCGGACGGGCGCCGTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTg                                                                                                                                           <  2:1764126/151‑1 (MQ=255)
        gggCGCTCCTCAGATCCATGAGTGACGCGCCGGACGGGCGCCGTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTg                                                                                                                                           <  2:10795742/151‑1 (MQ=255)
         ggCGCTCCTCAGATCCATGAGTGACGCGCCGGACGGGCGCCGTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGGACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTgg                                                                                                                                          <  1:5402180/151‑1 (MQ=255)
           cgcTCCTCAGATCCATGAGTGACGCGCCGGACGGGCGCCGTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGgcg                                                                                                                                        <  2:359786/151‑1 (MQ=255)
             ctcctcAGATCCATGAGTGACGCGCCGGACGGGCGCCGTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGcgcg                                                                                                                                      <  2:2884137/151‑1 (MQ=255)
             ctcctcAGATCCATGAGTGACGCGCCGGACGGGCGCCGTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGcgcg                                                                                                                                      >  1:6596841/1‑151 (MQ=255)
                ctcAGATCCATGAGTGACGCGCCGGACGGGCGCCGTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCt                                                                                                                                   <  2:5083811/151‑1 (MQ=255)
                    gATCCATGAGTGACGCGCCGGACGGGCGCCGTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCg                                                                                                                               >  2:435046/1‑151 (MQ=255)
                     aTCCATGAGTGACGCGCCGGACGGGCGCCGTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCgg                                                                                                                              >  1:5836175/1‑151 (MQ=255)
                     aTCCATGAGTGACGCGCCGGACGGGCGCCGTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCgg                                                                                                                              <  1:4218445/151‑1 (MQ=255)
                     aTCCATGAGTGACGCGCCGGACGGGCGCCGTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCgg                                                                                                                              <  2:5277400/151‑1 (MQ=255)
                     aTCCATGAGTGACGCGCCGGACGGGCGCCGTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGAACCGGGTCGTGCAGTGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCgg                                                                                                                              >  2:11102330/1‑151 (MQ=255)
                      tCCATGAGTGACGCGCCGGACGGGCGCCGTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGc                                                                                                                             <  1:6715186/151‑1 (MQ=255)
                         aTGAGTGACGCGCCGGACGGGCGCCGTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAg                                                                                                                          >  2:7337562/1‑151 (MQ=255)
                          tGAGTGACGCGCCGGACGGGCGCCGTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGc                                                                                                                         >  1:3743406/1‑151 (MQ=255)
                           gAGTGACGCGCCGGACGGGCGCCGTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCt                                                                                                                        <  1:9709766/151‑1 (MQ=255)
                            gctgtcgcgCCGGACGTGCGCCGTTCAGTTCGTGCACGCCTACCCCGCGGCCCTTCCCGCGTACCGGGGCGAGCAGGGTGGGGGTGCGGGCGTTGCGCCAGTGCCGGACGAGGTTGAACTCGGCGAGCGTGCCCCGGCTGCCGGCCAGCtc                                                                                                                       >  2:5422113/6‑150 (MQ=255)
                            aGTGACGCGCCGGACGGGCGCCGTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGGACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCtc                                                                                                                       <  2:5868246/151‑2 (MQ=255)
                               gACGCGCCGGACGGGCGCCGTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGaa                                                                                                                    <  1:7781186/151‑1 (MQ=255)
                                 cgcgCCGGACGGGCGCCGTTCAGGTAGGAGTCGCCGACCGCGTGGACCTTCCCGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGCGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAaga                                                                                                                  <  1:5157520/151‑1 (MQ=38)
                                  gcgcCGGACGGGCGCCGTCCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGTGGTTGAACTCGGCGTGCGTGGCGCGGCTGCCGGCCAGCTCGAagag                                                                                                                 <  1:11087337/151‑1 (MQ=25)
                                    gcCGGACGGGCGCCGTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCt                                                                                                               >  2:3101776/1‑151 (MQ=255)
                                     ccGGACGGGCGCCGTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCtt                                                                                                              <  2:6320687/151‑1 (MQ=255)
                                       ggACGGGCGCCGTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGTGCTtct                                                                                                            >  2:6722014/1‑151 (MQ=255)
                                       ggACGGGCGCCGTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTtct                                                                                                            <  2:5657079/151‑1 (MQ=255)
                                        gACGGGCGCCGTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTtctc                                                                                                           <  1:9594264/151‑1 (MQ=255)
                                            ggCGCCGTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTg                                                                                                       <  1:7167078/151‑1 (MQ=255)
                                             gcgcCGTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCGCGCTgg                                                                                                      >  1:10408869/1‑151 (MQ=255)
                                                ccGTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTggcgg                                                                                                   >  1:7413592/1‑151 (MQ=255)
                                                   ttCAGGTAGTAGTCGCCGCCCGCGTGGACCGTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGCCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCa                                                                                                <  1:4932941/151‑1 (MQ=25)
                                                   ttCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCa                                                                                                >  2:6183891/1‑151 (MQ=255)
                                                    tCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAg                                                                                               >  1:2276241/1‑151 (MQ=255)
                                                     cAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAgg                                                                                              <  1:7922348/151‑1 (MQ=255)
                                                       ggTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCt                                                                                            <  2:8354696/151‑1 (MQ=255)
                                                        gtagtaGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTg                                                                                           <  2:4056930/151‑1 (MQ=255)
                                                        gtagtaGACGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTg                                                                                           <  2:6405547/151‑1 (MQ=255)
                                                          agtagtCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGAt                                                                                         >  1:8809783/1‑151 (MQ=255)
                                                             agtCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGAtctc                                                                                      <  1:6265807/151‑1 (MQ=255)
                                                              gtCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCg                                                                                     >  1:3843965/1‑151 (MQ=255)
                                                              gtCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCg                                                                                     <  2:9545126/151‑1 (MQ=255)
                                                               tCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCgg                                                                                    >  1:1191659/1‑151 (MQ=255)
                                                                cGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGt                                                                                   <  2:9669922/151‑1 (MQ=255)
                                                                  ccGACCGCGTGGACCTTCCAGCTTACCGTGTCGTGCTGGGTGTGGGTGCGTGCGTTGCGCCAGTGCCGGTCGAGGATGAACTCGGCGAGCGTGTCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGc                                                                                 <  1:8156535/151‑1 (MQ=255)
                                                                   cGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCt                                                                                >  2:3448273/1‑151 (MQ=255)
                                                                    gACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTc                                                                               >  1:9840826/1‑151 (MQ=255)
                                                                    gACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTc                                                                               <  1:8401092/151‑1 (MQ=255)
                                                                     aCCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGTGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCa                                                                              >  2:4935665/1‑151 (MQ=255)
                                                                              aCCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTgg                                                                     <  2:6588012/151‑1 (MQ=255)
                                                                                cTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGcc                                                                   >  1:9384543/1‑151 (MQ=255)
                                                                                cTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGcc                                                                   >  2:8424434/1‑151 (MQ=255)
                                                                                  tCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTc                                                                 >  2:2444409/1‑151 (MQ=255)
                                                                                   ccAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCg                                                                <  1:2458606/151‑1 (MQ=255)
                                                                                     aGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGc                                                              >  1:1060774/1‑151 (MQ=255)
                                                                                        gTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCAc                                                           >  1:4277433/1‑151 (MQ=255)
                                                                                         tACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACg                                                          <  1:1994950/151‑1 (MQ=255)
                                                                                          aCCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACgg                                                         <  2:7645287/151‑1 (MQ=255)
                                                                                             gggTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGa                                                      >  1:916284/1‑151 (MQ=255)
                                                                                               gTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATc                                                    <  1:4032139/151‑1 (MQ=255)
                                                                                                tCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCg                                                   <  1:4523359/151‑1 (MQ=255)
                                                                                                 cGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGa                                                  >  2:142365/1‑151 (MQ=255)
                                                                                                 cGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGa                                                  <  1:10754430/151‑1 (MQ=255)
                                                                                                  gTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAg                                                 <  2:4706098/151‑1 (MQ=255)
                                                                                                    gCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGgc                                               <  2:10036765/151‑1 (MQ=255)
                                                                                                       ggggGTGGGTGCGGGCGTTGCGCCAGAGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAATAGCTTCTCGCTCGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGcgcg                                            >  2:11188882/1‑151 (MQ=37)
                                                                                                         gtgtGGGTGCGGGCGTTGCGCCTGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGTCGATCGAGGCGCGTg                                          <  2:6778520/151‑1 (MQ=255)
                                                                                                           gtgGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCTGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTgcc                                        <  2:4464429/151‑1 (MQ=255)
                                                                                                             gggTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTgccgc                                      <  2:7931813/151‑1 (MQ=255)
                                                                                                              ggTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTgccgcc                                     >  1:265299/1‑151 (MQ=255)
                                                                                                              ggTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTgccgcc                                     <  2:5358992/151‑1 (MQ=255)
                                                                                                              ggTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTgccgcc                                     <  1:3013500/151‑1 (MQ=255)
                                                                                                                 gCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGAc                                  <  1:7643865/151‑1 (MQ=255)
                                                                                                                  cGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACt                                 >  2:2010913/1‑151 (MQ=255)
                                                                                                                   gggCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTc                                >  2:10192101/1‑151 (MQ=255)
                                                                                                                    ggCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTCg                               <  1:7279601/151‑1 (MQ=255)
                                                                                                                     gCGTTGCGCCAGTGCCGGTCGAGGTTGACCTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTCgg                              <  2:10518904/151‑1 (MQ=255)
                                                                                                                       gTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTCGGCg                            >  1:1474826/1‑151 (MQ=255)
                                                                                                                              cAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCTCCGACTCGGCGTCCACCg                     <  2:9011285/151‑1 (MQ=255)
                                                                                                                              cAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTCGGCGTCCACCg                     >  2:2152523/1‑151 (MQ=255)
                                                                                                                              cAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTCGGCGTCCACCg                     >  2:11382/1‑151 (MQ=255)
                                                                                                                                 tGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCGTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTCGGCGTCCACCGGcc                  <  2:3878553/151‑1 (MQ=255)
                                                                                                                                  gCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTCGGCGTCCACCGGCcg                 >  1:6960339/1‑151 (MQ=255)
                                                                                                                                    cGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTCGGCGTCCCCCGGCcgcg               >  1:9657677/1‑151 (MQ=255)
                                                                                                                                    cGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTCGGCGTCCACCGGCcgcg               >  2:7043352/1‑151 (MQ=255)
                                                                                                                                      gtcgaggttgaACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTCGGCGTCCACCGGCcgcgcg             <  2:8715468/148‑1 (MQ=255)
                                                                                                                                       tcgaggttgaACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTCGGCGTCCACCGGCCGCGCgg            >  2:8107296/3‑151 (MQ=255)
                                                                                                                                         gaggttgaACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGACACGGCGATCGAGGCGCGTGCCGCCGACTCGGCGTCCACCGGCCGCGCGGcc          >  1:3224156/1‑151 (MQ=255)
                                                                                                                                         gaggttgaACTCGGCGAGCGAGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTCGGCGTCCACCGGCCGCGCGGcc          <  1:6352203/151‑1 (MQ=255)
                                                                                                                                              tgaACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTCGGCGTCCACCGGCCGCGCGGCCACTtc     >  1:3585860/1‑151 (MQ=255)
                                                                                                                                               gaACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTCGGCGTCCACCGGCCGCGCGGCCACTtcg    <  2:9431910/151‑1 (MQ=255)
                                                                                                                                                aaCTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTCGGCGTCCACCGGCCGCGCGGCCACTtcgt   >  1:574428/1‑151 (MQ=255)
                                                                                                                                                aaCTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTCGGCGTCCACCGGCCGCGCGGCCACTtcgt   >  1:10095887/1‑151 (MQ=255)
                                                                                                                                                 aCTCGGCGAGCGTGGCGCGTCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTCGGCGTCCACCGGCCGCGCGGCCACTtcgtc  <  1:8522285/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GGCTCATCGGGCGCTCCTCAGATCCATGAGTGACGCGCCGGACGGGCGCCGTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTTGAGGTTGAACTTGGCGAGCGTGGCGCGGCTGCCGGCCAGCTTGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTCGGCGTCCACCGGCCGCGCGGCCACTTCGTC  >  NC_008463/3051265‑3051560

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑