Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 3,051,443 T→C K359E (AAG→GAG)  PA14_RS13990 ← SfnB family sulfur acquisition oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084633,051,4430TC100.0% 2103.5 / NA 546K359E (AAG→GAG) PA14_RS13990SfnB family sulfur acquisition oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (269/277);  total (269/277)

Only 100 of 546 total aligned reads displayed.

TGACGCGCCGGACGGGCGCCGTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTTGAGGTTGAACTTGGCGAGCGTGGCGCGGCTGCCGGCCAGCTTGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTCGGCGTCCACCGGCCGCGCGGCCACTTCGTCGAGGACCTGGCCGGCCTTCTCCAGCAGGGC  >  NC_008463/3051295‑3051590
                                                                                                                                                    |                                                                                                                                                   
tGACGCGCCGGACGGGCGCCGTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGa                                                                                                                                                   <  2:2059478/151‑1 (MQ=255)
    gcgcCGGACGGGCGCCGTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGAAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAagag                                                                                                                                               >  1:641721/1‑151 (MQ=255)
      gcCGGACGGGCGCCGTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCt                                                                                                                                             <  2:610141/151‑1 (MQ=255)
         ggACGGGCGCCGTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGTCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTtct                                                                                                                                          <  2:5743100/151‑1 (MQ=255)
         ggACGGGCGCCGTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGTGCTtct                                                                                                                                          >  2:6722014/1‑151 (MQ=255)
           aCGGGCGCCGTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCg                                                                                                                                        >  1:9021008/1‑151 (MQ=255)
            cGGGCGCCGTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGc                                                                                                                                       <  2:9750328/151‑1 (MQ=255)
               gcgcCGTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTgg                                                                                                                                    <  2:10804389/151‑1 (MQ=255)
                cgcCGTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTggc                                                                                                                                   <  1:7610280/151‑1 (MQ=255)
                 gcCGTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTggcg                                                                                                                                  >  1:10905128/1‑151 (MQ=255)
                     ttCAGGTAGTAGTCGCCGCCCGCGTGGACCGTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGCCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCa                                                                                                                              <  1:4932941/151‑1 (MQ=25)
                     ttCAGGTAGTAGTCGCCGACCGCGAGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCTGTGCCGGTCGAGGTTGTACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCa                                                                                                                              <  1:724044/151‑1 (MQ=25)
                      tCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAg                                                                                                                             >  2:9446909/1‑151 (MQ=255)
                       cAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAgg                                                                                                                            <  1:7805110/151‑1 (MQ=255)
                        aGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGACCTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGc                                                                                                                           >  2:10367733/1‑151 (MQ=255)
                        aGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGc                                                                                                                           >  1:7384399/1‑151 (MQ=255)
                        aGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGc                                                                                                                           >  2:4859336/1‑151 (MQ=255)
                         ggTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCt                                                                                                                          >  1:8127632/1‑151 (MQ=255)
                            agtagtCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGAt                                                                                                                       >  2:270835/1‑151 (MQ=255)
                            agtagtCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGAt                                                                                                                       >  1:8809670/1‑151 (MQ=255)
                            agtagtCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGAt                                                                                                                       >  1:5230435/1‑151 (MQ=255)
                                gtCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCg                                                                                                                   <  2:3432799/151‑1 (MQ=255)
                                   gCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTg                                                                                                                >  1:11253235/1‑151 (MQ=255)
                                     cGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCt                                                                                                              >  1:3632292/1‑151 (MQ=255)
                                      gACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTc                                                                                                             >  2:5344879/1‑151 (MQ=255)
                                            gTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGATCTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCAcc                                                                                                       <  1:2979039/151‑1 (MQ=255)
                                             tGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCt                                                                                                      <  1:5905166/151‑1 (MQ=255)
                                               gACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTg                                                                                                    >  1:9562584/1‑151 (MQ=255)
                                                aCCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTgg                                                                                                   <  2:1014181/151‑1 (MQ=255)
                                                aCCGTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTgg                                                                                                   <  2:6588030/151‑1 (MQ=255)
                                                  cTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGcc                                                                                                 >  2:6463190/1‑151 (MQ=255)
                                                  cTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGcc                                                                                                 >  1:9376063/1‑151 (MQ=255)
                                                   ttCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCt                                                                                                >  2:11178247/1‑151 (MQ=255)
                                                     ccAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCg                                                                                              <  1:2458606/151‑1 (MQ=255)
                                                     ccAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCg                                                                                              >  1:6257956/1‑151 (MQ=255)
                                                     ccAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCg                                                                                              <  1:9840400/151‑1 (MQ=255)
                                                       aGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGc                                                                                            >  1:4493556/1‑151 (MQ=255)
                                                          gTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCAc                                                                                         >  2:7273600/1‑151 (MQ=255)
                                                          gTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCAc                                                                                         >  1:4277433/1‑151 (MQ=255)
                                                          gTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCAc                                                                                         >  1:1028106/1‑151 (MQ=255)
                                                          gTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCAc                                                                                         >  1:10444051/1‑151 (MQ=255)
                                                           tACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACg                                                                                        <  1:1994950/151‑1 (MQ=255)
                                                            aCCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACgg                                                                                       >  2:429243/1‑151 (MQ=255)
                                                               gggTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGa                                                                                    >  1:4089666/1‑151 (MQ=255)
                                                                 gTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATc                                                                                  >  2:1889752/1‑151 (MQ=255)
                                                                   cGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGa                                                                                >  2:10005837/1‑151 (MQ=255)
                                                                    gTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAg                                                                               <  1:7763587/151‑1 (MQ=255)
                                                                    gTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAg                                                                               <  2:6276292/151‑1 (MQ=255)
                                                                      gCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGgc                                                                             <  1:5292300/151‑1 (MQ=255)
                                                                        aGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGCAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGgcgc                                                                           <  1:2352300/151‑1 (MQ=255)
                                                                         gggTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGcgcg                                                                          <  1:8950721/151‑1 (MQ=255)
                                                                           gtgtGGGTGCGGGCGTTGCGCCTGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGTCGATCGAGGCGCGTg                                                                        <  2:6778520/151‑1 (MQ=255)
                                                                           gtgtGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTg                                                                        >  2:9598145/1‑151 (MQ=255)
                                                                               gggTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTgccgc                                                                    <  2:7931813/151‑1 (MQ=255)
                                                                                  tGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGa                                                                 >  1:5776159/1‑151 (MQ=255)
                                                                                  tGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGa                                                                 <  1:3899118/151‑1 (MQ=255)
                                                                                     gggCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTc                                                              <  2:4412092/151‑1 (MQ=255)
                                                                                       gCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTCgg                                                            <  2:10518961/151‑1 (MQ=255)
                                                                                       gCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTCgg                                                            >  1:8396540/1‑151 (MQ=255)
                                                                                        cggtGCGCCAGTGCCGGACGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGACGAGCTTCTCGCTGGCGGCCAGGCTGCTCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGCGGCGCGTGCCGCCGACTCGGc                                                           <  1:3513010/148‑1 (MQ=38)
                                                                                           tGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTCGGCGTc                                                        <  2:10198695/151‑1 (MQ=255)
                                                                                              gcCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTCGGCGTCCAc                                                     >  2:929014/1‑151 (MQ=255)
                                                                                               ccAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTCGGCGTCCAcc                                                    <  2:877520/151‑1 (MQ=255)
                                                                                                cAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGACGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCATGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTCGGCGTCCACCg                                                   <  2:2502709/151‑1 (MQ=255)
                                                                                                   tGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTCGGCGTCCACCGGcc                                                <  2:3224145/151‑1 (MQ=255)
                                                                                                   tGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTCGGCGTCCACCGGcc                                                <  2:7506471/151‑1 (MQ=255)
                                                                                                   tGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCGTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTCGGCGTCCACCGGcc                                                <  2:3878553/151‑1 (MQ=255)
                                                                                                    gCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTCGGCGTCCACCGGCcg                                               >  1:6949235/1‑151 (MQ=255)
                                                                                                      cGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTCGGCGTCCACCGGCcgcg                                             >  1:9657633/1‑151 (MQ=255)
                                                                                                      cGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTCGGCGTCCACCGGCcgcg                                             >  2:7043352/1‑151 (MQ=255)
                                                                                                        gtcgaggttgaACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTCGGCGTCCACCGGCcgcgcg                                           <  2:8977602/148‑1 (MQ=255)
                                                                                                         tcgaggttgaACTCGGCGCGCGTGGCGCGGCTGCCGGCCAGCACGAAGAGCTTCTCGCTGGCGGCCAGGCAGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGCCTCGGCGTCCACCGGCCGCGCgg                                          <  2:5933390/149‑1 (MQ=39)
                                                                                                         tcgaggttgaACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTCGGCGTCCACCGGCCGCGCgg                                          >  2:8107296/3‑151 (MQ=255)
                                                                                                          cgaggttgaACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTCGGCGTCCACCGGCCGCGCGGc                                         >  2:2330698/2‑151 (MQ=255)
                                                                                                           gaggttgaACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTCGGCGTCCACCGGCCGCGCGGcc                                        <  1:6351967/151‑1 (MQ=255)
                                                                                                            aggttgaACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTCGGCGTCCACCGGCCGCGCGGCCa                                       >  1:1324666/1‑151 (MQ=255)
                                                                                                               ttgaACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTCGGCGTCCACCGGCCGCGCGGCCACtt                                    <  1:10611711/151‑1 (MQ=255)
                                                                                                                   aCTCGGCGAGCGTGGCGCGTCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTCGGCGTCCACCGGCCGCGCGGCCACTtcgtc                                <  1:8522285/151‑1 (MQ=255)
                                                                                                                    ctcggCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACGCGGCGTCCACCGGCCGCGCGGCCACTtcgtcg                               >  2:2972880/4‑151 (MQ=255)
                                                                                                                     tcggCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTCGGCGTCCACCGGCCGCGCGGCCACTTCGTCGa                              <  1:4719617/149‑1 (MQ=255)
                                                                                                                         cGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCGGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTCGGCGTCCACCGGCCGCGCGGCCACTTCGTCGAGGAc                          <  2:8396582/151‑1 (MQ=255)
                                                                                                                           aGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTCGGCGTCCACCGGCCGCGCGGCCACTTCGTCGAGGACCt                        >  1:9699284/1‑151 (MQ=255)
                                                                                                                            gCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTCGGCGTCCACCGGCCGCGCGGCCACTTCGTCGAGGACCTg                       <  2:5588190/151‑1 (MQ=255)
                                                                                                                            gCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTCGGCGTCCACCGGCCGCGCGGCCACTTCGTCGAGGACCTg                       <  2:8329126/151‑1 (MQ=255)
                                                                                                                                 gcgcGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTCGGCGTCCACCGGCCGCGCGGCCACTTCGTCGAGGACCTggccgg                  <  1:1735354/151‑1 (MQ=255)
                                                                                                                                   gcgGCTGCCGGCCCGCTCGAAGAGCGTCTCGCAGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTCGGCGTCCACCGGCCGCGCGGCCACTTCGTCGACGACCTggccggcc                <  2:1628168/151‑1 (MQ=255)
                                                                                                                                   gcgGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTCGGCGTCCACCGGCCGCGCGGCCACTTCGTCGAGGACCTggccggcc                <  2:5465865/151‑1 (MQ=255)
                                                                                                                                    cgGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTCGGCGTCCACCGGCCGCGCGGCCACTTCGTCGAGGACCTGGCCGGCCt               >  1:7056221/1‑151 (MQ=255)
                                                                                                                                    cgGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTCGGCGTCCACCGGCCGCGCGGCCACTTCGTCGAGGACCTGGCCGGCCt               <  1:5526240/151‑1 (MQ=255)
                                                                                                                                     ggCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGGGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTCGGCGTCCACCGGCCGCGCGGCCACTTCGTCGAGGACCTGGCCGGCCtt              <  1:2857440/151‑1 (MQ=255)
                                                                                                                                        tGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTCGGCGTCCACCGGCCGCGCGGCCACTTCGTCGAGGACCTGGCCGGCCTtctc           <  1:6820323/151‑1 (MQ=255)
                                                                                                                                        tGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTCGGCGTCCACCGGCCGCGCGGCCACTTCGTCGAGGACCTGGCCGGCCTtctc           >  2:4581704/1‑151 (MQ=255)
                                                                                                                                          ccGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTCGGCGTCCACCGGCCGCGCGGCCACTTCGTCGAGGACCTGGCCGGCCTTCTCca         >  1:10006823/1‑151 (MQ=255)
                                                                                                                                          ccGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTCGGCGTCCACCGGCCGCGCGGCCACTTCGTCGAGGACCTGGCCGGCCTTCTCca         >  2:3684380/1‑151 (MQ=255)
                                                                                                                                           cGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTCGGCGTCCACCGGCCGCGCGGCCACTTCGTCGAGGACCTGGCCGGCCTTCTCcag        >  2:5189283/1‑151 (MQ=255)
                                                                                                                                              ccAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTCGGCGTCCACCGGCCGCGCGGCCACTTCGTCGAGGACCTGGCCGGCCTTCTCcagcag     <  2:7673552/151‑1 (MQ=255)
                                                                                                                                              ccAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTCGGCGTCCACCGGCCGCGCGGCCACTTCGTCGAGGACCTGGCCGGCCTTCTCcagcag     <  2:1134154/151‑1 (MQ=255)
                                                                                                                                                aGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTCGGCGTCCACCGGCCGCGCGGCCACTTCGTCGAGGACCTGGCCGGCCTTCTCCAGCAggg   <  1:2707536/151‑1 (MQ=255)
                                                                                                                                                 gCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTCGGCGTCCACCGGCCGCGCGGCCACTTCGTCGAGGACCTGGCCGGCCTTCTCCAGCAGGGc  <  2:8672550/151‑1 (MQ=255)
                                                                                                                                                 gCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTCGGCGTCCACCGGCCGCGCGGCCACTTCGTCGAGGACCTGGCCGGCCTTCTCCAGCAGGGc  >  2:5002438/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
TGACGCGCCGGACGGGCGCCGTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTTGAGGTTGAACTTGGCGAGCGTGGCGCGGCTGCCGGCCAGCTTGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTCGGCGTCCACCGGCCGCGCGGCCACTTCGTCGAGGACCTGGCCGGCCTTCTCCAGCAGGGC  >  NC_008463/3051295‑3051590

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑