Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 3,198,441 T→G A547A (GCA→GCC)  PA14_RS14615 ← acyl‑CoA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084633,198,4410TG100.0% 1460.6 / NA 384A547A (GCA→GCC) PA14_RS14615acyl‑CoA synthetase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (246/138);  total (246/138)

Only 100 of 387 total aligned reads displayed.

TCAGGGTGAAACGGTTTCGGCGCGCCACCAGTTGCTGGAACCGGGCACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCTGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCTTGAAGTGCGCCAGGCGCTCGCGGCACCAGCGGACCAG  >  NC_008463/3198294‑3198587
                                                                                                                                                   |                                                                                                                                                  
tCAGGGTGAAACGGTTTCGGCGCGCCACCAGTTGCTGGAACCGGGCACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCg                                                                                                                                                 <  2:1989635/151‑1 (MQ=255)
tCAGGGTGAAACGGTTTCGGCGCGCCACCAGTTGCTGGAACCGGGCACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATGGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCg                                                                                                                                                 >  1:4223291/1‑151 (MQ=255)
  aGGGTGAAACGGTTTCGGCGCGCCACCAGTTGCTGGAACCGGGCACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTc                                                                                                                                               >  1:4486616/1‑151 (MQ=255)
      tGAAACGGTTTCGGCGCGCCACCAGTTGCTGGAACCGGGCACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTTGGTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCg                                                                                                                                           >  1:918373/1‑151 (MQ=255)
         aaCGGTTTCGGCGCGCCACCAGTTGCTGGAACCGGGCACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGcc                                                                                                                                        >  2:4096887/1‑151 (MQ=255)
               ttCGGCGCGCCACCAGTTGCTGGAACCGGGCACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGcctcctcc                                                                                                                                  <  2:10638056/151‑1 (MQ=255)
               ttCGGCGCGCCACCAGTTGCTGGAACCGGGCACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGcctcctcc                                                                                                                                  >  1:1224850/1‑151 (MQ=255)
                  ggCGCGCCACCAGTTGCTGGAACCGGGCACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGcctcctcctcc                                                                                                                               <  1:2560307/151‑1 (MQ=255)
                     gcgcCACCAGTTGCTGGAACCGGGCACTGGCGCGGATACGTGCGTATAGCAGGCTGGTCATGGGACACCTCGGGCCACCTAGGTATGGTTAGGGAAGGTGCGGGTGCGGCAGCGCGATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGc                                                                                                                            >  2:1669517/1‑151 (MQ=255)
                     gcgcCACCAGTTGCTGGAACCGGGCACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGc                                                                                                                            >  1:1462730/1‑151 (MQ=255)
                      cgcCACCAGTTGCTGGAACCGGGCACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGcc                                                                                                                           >  2:8518593/1‑151 (MQ=255)
                         caccaGTTGCTGGAACCGGGCACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCggg                                                                                                                        >  1:5050139/1‑151 (MQ=255)
                           ccaGTTGCTGGAACCGGGCACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGcc                                                                                                                      <  2:10548797/151‑1 (MQ=255)
                            caGTTGCTGGAACCGGGCACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGccc                                                                                                                     >  1:278183/1‑151 (MQ=255)
                             aGTTGCTGGAACCGGGCACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCa                                                                                                                    >  2:3165247/1‑151 (MQ=255)
                                tGCTGGAACCGGGCACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTc                                                                                                                 >  2:7555019/1‑151 (MQ=255)
                                 gCTGGAACCGGGCACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTcg                                                                                                                >  2:10105153/1‑151 (MQ=255)
                                  cTGGAACCGGGCACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTcgc                                                                                                               >  1:9717482/1‑151 (MQ=255)
                                   tGGAACCGGGCACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTcgcg                                                                                                              >  1:7054493/1‑151 (MQ=255)
                                   tGGAACCGGGCACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGATATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTcgcg                                                                                                              >  2:9321515/1‑151 (MQ=255)
                                     gAACCGGGCACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCa                                                                                                            <  2:7254142/151‑1 (MQ=255)
                                      aaCCGGGCACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAg                                                                                                           >  2:2634128/1‑151 (MQ=255)
                                      aaCCGGGCACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAg                                                                                                           <  2:8711015/151‑1 (MQ=255)
                                         cGGGCACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGAc                                                                                                        >  1:2375104/1‑151 (MQ=255)
                                          gggCACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACg                                                                                                       >  2:5691802/1‑151 (MQ=255)
                                           ggCACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGa                                                                                                      >  1:5635429/1‑151 (MQ=255)
                                           ggCACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGa                                                                                                      >  2:1208803/1‑151 (MQ=255)
                                            gCACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATGGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGTCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGaa                                                                                                     >  1:8683554/1‑151 (MQ=255)
                                              aCTGTCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACt                                                                                                   >  2:2283062/1‑151 (MQ=255)
                                              aCTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACt                                                                                                   >  1:5140068/1‑151 (MQ=255)
                                                tGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTc                                                                                                 <  1:4901715/151‑1 (MQ=255)
                                                 ggCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCt                                                                                                <  2:2419629/151‑1 (MQ=255)
                                                    gcgGATAAGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGGTATTGATCTGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGCCGAACTTCTGGa                                                                                             >  1:3836732/1‑151 (MQ=255)
                                                     cgGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGGTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGAt                                                                                            <  2:3292436/151‑1 (MQ=255)
                                                        aTACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCtt                                                                                         >  2:492954/1‑151 (MQ=255)
                                                         tACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTg                                                                                        >  1:3066960/1‑151 (MQ=255)
                                                           cGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGcc                                                                                      <  1:3183783/151‑1 (MQ=255)
                                                            gTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCg                                                                                     >  1:6199035/1‑151 (MQ=255)
                                                            gTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCGCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTACTGGATCTTGCCg                                                                                     >  2:1034680/1‑151 (MQ=255)
                                                                 tataGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGc                                                                                <  2:6484898/151‑1 (MQ=255)
                                                                  ataGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCg                                                                               <  1:3301695/151‑1 (MQ=255)
                                                                  ataGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCg                                                                               >  1:7734509/1‑151 (MQ=255)
                                                                     gCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTc                                                                            >  1:5817800/1‑151 (MQ=255)
                                                                      cAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCt                                                                           >  1:10912924/1‑151 (MQ=255)
                                                                        ggCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTg                                                                         >  1:5361752/1‑151 (MQ=255)
                                                                         gCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTgg                                                                        >  2:4707592/1‑151 (MQ=255)
                                                                          cTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTCTGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACGCGCGCAGGACGAACGTCTGGATCTTGCCGGTGGCGGGCTTggg                                                                       >  2:11092609/1‑151 (MQ=255)
                                                                            ggACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCa                                                                     <  2:1247888/151‑1 (MQ=255)
                                                                               cATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGtt                                                                  >  1:10081990/1‑151 (MQ=255)
                                                                                aTGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTc                                                                 >  1:7922570/1‑151 (MQ=255)
                                                                                 tGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCg                                                                >  1:6164340/1‑151 (MQ=255)
                                                                                   ggACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGt                                                              >  1:11084069/1‑151 (MQ=255)
                                                                                    gACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTa                                                             >  2:8043468/1‑151 (MQ=255)
                                                                                     acacCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTaa                                                            <  1:5296926/151‑1 (MQ=255)
                                                                                     acacCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTaa                                                            <  2:8997753/151‑1 (MQ=255)
                                                                                      cacCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTGGGGCAGTTCGGTAAg                                                           >  1:6599849/1‑151 (MQ=255)
                                                                                       acCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGc                                                          <  1:10762041/151‑1 (MQ=255)
                                                                                        ccTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCg                                                         <  2:3754016/151‑1 (MQ=255)
                                                                                             ggCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTcaca                                                    >  2:4531694/1‑151 (MQ=255)
                                                                                             ggCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTcaca                                                    <  1:4071520/151‑1 (MQ=255)
                                                                                             ggCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTcaca                                                    >  1:2487551/1‑151 (MQ=255)
                                                                                                 aCCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGcc                                                >  2:8823017/1‑151 (MQ=255)
                                                                                                  ccTAGTTATTGTTATTGTATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTTAGCGTCACATGCcg                                               <  2:5100092/151‑1 (MQ=255)
                                                                                                   cTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCcgc                                              >  1:10292417/1‑151 (MQ=255)
                                                                                                     aGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCgcgg                                            <  2:9762155/151‑1 (MQ=255)
                                                                                                       ttATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGTACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGGTCGGTAAGCGTCACATGCCgcggcg                                          >  2:1746491/1‑151 (MQ=255)
                                                                                                          ttGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCt                                       <  2:4756534/151‑1 (MQ=255)
                                                                                                             ttATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCTTGa                                    >  1:8217964/1‑151 (MQ=255)
                                                                                                             ttATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCTTGa                                    >  1:7368526/1‑151 (MQ=255)
                                                                                                              tATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCTTGaa                                   >  2:9484965/1‑151 (MQ=255)
                                                                                                              tATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCTTGaa                                   >  2:3459201/1‑151 (MQ=255)
                                                                                                               aTGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCTTGAAg                                  >  1:363702/1‑151 (MQ=255)
                                                                                                               aTGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCTTGAAg                                  >  1:5942297/1‑151 (MQ=255)
                                                                                                                  gAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCTTGAAGTgc                               >  2:6671993/1‑151 (MQ=255)
                                                                                                                  gAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTACGGTAAGCGTCACATGCCGCGGCGCCTTGAAGTgc                               >  1:1438174/1‑151 (MQ=255)
                                                                                                                   aaTGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCTTGAAGTgcg                              <  1:2091276/151‑1 (MQ=255)
                                                                                                                    aTGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGGCACATGCCGCGGCGCCTTGAAGTgcgc                             >  2:6098388/1‑151 (MQ=255)
                                                                                                                      gtgcgggtgcggCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCTTGAAGTGCGCCa                           >  2:3609078/1‑151 (MQ=255)
                                                                                                                       tgcgggtgcggCAGCGCCATCAGCGGCCGGCGTCGCAGGCCTCCACCTCCTGCCGGGCCCACTCGCTCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTAGGTAAGCTTCACATGCCGCGGCGACTTGAAGTGCGCCAg                          >  2:3371308/1‑151 (MQ=255)
                                                                                                                          gggtgcggCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCTTGAAGTGCGCCAGgcg                       <  1:928206/151‑1 (MQ=255)
                                                                                                                            gtgcggCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCTTGACGTGCGCCAGGCGCt                     >  1:7512171/1‑151 (MQ=255)
                                                                                                                            gtgcggCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCTTGAAGTGCGCCAGGCGCt                     <  1:8093898/151‑1 (MQ=255)
                                                                                                                                ggCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCTTGAAGTGCGCCAGGCGCTcgcg                 >  1:6903317/1‑151 (MQ=255)
                                                                                                                                ggCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCTTGAAGTGCGCCAGGCGCTcgcg                 >  2:5506803/1‑151 (MQ=255)
                                                                                                                                ggCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCTGGAAGTGCGCCAGGCGCTcgcg                 >  2:2949183/1‑151 (MQ=255)
                                                                                                                                ggCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGCAAGCGTCACATGCCGCGGCGCCTTGAAGTGCGCCAGGCGCTcgcg                 >  1:675425/1‑151 (MQ=255)
                                                                                                                                   aGCGCCATCAGCGGCCGGCGTCGCCGGCGTCCTCCTCCTGCCGGGGCCTCTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTGCGGTAAGCGTCACATGGCGCGGCGCGTGGTTGTGCGCCAGGCGCTGGCGGCa              >  2:10800632/1‑151 (MQ=255)
                                                                                                                                   aGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCTGGACGAACTTCTGGTTCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCTTGAAGTGCGCCAGGCGCTCGCGGct              >  2:1875713/1‑150 (MQ=255)
                                                                                                                                   aGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGACCTTCTGGATCTTGCCGGTGGCGGTCTGGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCGTGAAGTGCGCCAGGCGCTCGCGGCa              >  2:5241367/1‑151 (MQ=255)
                                                                                                                                   aGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCTTGAAGTGCGCCAGGCGCTCGCGGCa              >  2:6223166/1‑151 (MQ=255)
                                                                                                                                      gcCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCTTGAAGTGCGCCAGTCGCTCGCGGCACCa           <  1:940588/151‑1 (MQ=255)
                                                                                                                                       ccATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCTTGAAGTGCGCCAGGCGCTCGCGGCACCAg          >  2:5990182/1‑151 (MQ=255)
                                                                                                                                        cATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGACCTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCATGAAGTGCGCCAGGCGCTCGCGGCACCAGc         <  1:4673283/151‑1 (MQ=255)
                                                                                                                                        cATCAGCGGCCGGCGTCGCCGGCCTCATCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCTTGAAGTGCGCCAGGCGCTCGCGGCACCAGc         >  1:8513715/1‑151 (MQ=255)
                                                                                                                                         aTCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCTTGAAGTGCGCCAGGCGCTCGCGGCACCAGCg        >  1:3005108/1‑151 (MQ=255)
                                                                                                                                          tCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCTTGAAGTGCGCCAGGCGCTCGCGGCACCAGCgg       <  1:6023027/151‑1 (MQ=255)
                                                                                                                                           cAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCTTGAAGTGCGCCAGGCGCTCGCGGCACCAGCGGa      <  2:7752427/151‑1 (MQ=255)
                                                                                                                                           cAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCTTGAAGTGCGCCAGGCGCTCGCGGCACCAGCGGa      <  2:2131728/151‑1 (MQ=255)
                                                                                                                                              cggccggcGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCTTGAAGTGCGCCAGGCGCTCGCGGCACCAGCGGACCa   <  2:8594611/151‑1 (MQ=255)
                                                                                                                                               ggCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCTTGAAGTGCGCCAGGCGCTCGCGGCACCAGCGGACCAg  >  1:9970426/1‑151 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TCAGGGTGAAACGGTTTCGGCGCGCCACCAGTTGCTGGAACCGGGCACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCTGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCTTGAAGTGCGCCAGGCGCTCGCGGCACCAGCGGACCAG  >  NC_008463/3198294‑3198587

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑