BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_008463	5,809,365	T→C	K239E (AAG→GAG)	rlmB ←	23S rRNA (guanosine(2251)‑2'‑O)‑methyltransferase RlmB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_008463	5,809,365	0	T	C	100.0%	1662.6 / NA	443	K239E (AAG→GAG)	rlmB	23S rRNA (guanosine(2251)‑2'‑O)‑methyltransferase RlmB
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (247/196); total (247/196)

Only 100 of 444 total aligned reads displayed.

GCGGCAAGGGGCGACATTCTAGAGAAGGGCAACCGGCGAAGCAAGGAAGATTGGCGAATATTTGTACAGCGGATGGAATGGGCGCGCGGGGCCCCCCCCGGGGGGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTTGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGCCTTCCGCGCCCATCACCAGCACGGTCGGCCCCGT  >  NC_008463/5809215‑5809512

gccgCAAGGGTCGACATACTAGAGAAGGCCCACCGGCGAAGCAAGGAAGATTGGCGAATCATAGTCCAGCGGATGGAATG‑‑‑GCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGAGCGCTGGCGGAGGATCTCGAAc                                                                                                                                                 <  2:6679978/148‑1 (MQ=255)

   gcggcaaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAAc                                                                                                                                                 >  1:7207896‑M2/82‑151 (MQ=255)

       caaggggcgacattctagagcagggcaaccggcgaagcaaggaagcttggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGc                                                                                                                                             <  2:5022483‑M2/74‑1 (MQ=255)

       caaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGc                                                                                                                                             >  2:6455108‑M2/78‑151 (MQ=255)

             gcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGc                                                                                                                                       >  1:4116301‑M2/72‑151 (MQ=255)

                 cattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGt                                                                                                                                   >  1:8005645‑M2/68‑151 (MQ=255)

                    tctagagaagggcacccggcgacgcaaggaagagtggcgaatatttctacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGc                                                                                                                                <  2:4947636‑M2/87‑1 (MQ=255)

                    tctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGc                                                                                                                                <  1:4051944‑M2/87‑1 (MQ=255)

                     ctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCCGCGGGGTGCGCTGGCGGAGGGTCGCGAACAGGCAGACGCCGGTGGCg                                                                                                                               >  1:10600656‑M2/64‑151 (MQ=255)

                     ctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCg                                                                                                                               <  1:2566018‑M2/88‑1 (MQ=255)

                       agagaagggcacccggcgaagcacggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGAc                                                                                                                             <  1:8434312‑M2/90‑1 (MQ=255)

                       agagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggagggcatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCCCGCGCTCACTCCCGCGGGGTGCGCTGGCGGAGGATCGCGAACAGGCCGACGCCGGTGGCGAc                                                                                                                             >  2:6123491‑M2/62‑151 (MQ=255)

                       agagaagggcaaccggcgaagcaaggaagatgggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGAc                                                                                                                             <  2:10641079‑M2/90‑1 (MQ=255)

                        gagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGAcc                                                                                                                            >  2:2160668‑M2/61‑151 (MQ=255)

                        gagaagggcaaccggcgaagcaaggaagatgggcgaatatgggtaccgcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGccc                                                                                                                            <  1:8835021‑M2/91‑1 (MQ=255)

                            agggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCgaga                                                                                                                        >  1:10367875‑M2/57‑151 (MQ=255)

                              ggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACg                                                                                                                      >  2:6992789‑M2/55‑151 (MQ=255)

                                caaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGtt                                                                                                                    >  1:9224393‑M2/53‑151 (MQ=255)

                                caaccggagaagcaaggaagattggctaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGtt                                                                                                                    <  1:7526360‑M2/99‑1 (MQ=255)

                                  accggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCa                                                                                                                  >  1:8724202‑M2/51‑151 (MQ=255)

                                    cggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAgg                                                                                                                <  1:3274349‑M2/103‑1 (MQ=255)

                                    cggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAgg                                                                                                                >  2:2267561‑M2/49‑151 (MQ=255)

                                     ggcgcagcaaggaagattggcgaatatttgtacagcggttggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCTCTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGgc                                                                                                               <  2:2027510‑M2/104‑1 (MQ=255)

                                      gcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGgct                                                                                                              >  2:3560229‑M2/47‑151 (MQ=255)

                                        gaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGGCCGCGCCC‑CGGCGGCCCCGCACGCGCACACACCAGCGGGGTGCGCTGGCGGAGGATCACGAACAGGCACACGCCGGTCGCGACCCAGACGATCAGgcagc                                                                                                            >  1:6549765‑M2/45‑151 (MQ=255)

                                         aagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGgctgct                                                                                                           <  1:9716130‑M2/108‑1 (MQ=255)

                                            caaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGAc                                                                                                        <  1:10014259‑M2/111‑1 (MQ=255)

                                              aggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGc                                                                                                      <  1:10274497‑M2/113‑1 (MQ=255)

                                              aggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGc                                                                                                      <  1:5581773‑M2/113‑1 (MQ=255)

                                               ggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCt                                                                                                     <  2:4453786‑M2/114‑1 (MQ=255)

                                                 aagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGc                                                                                                   <  2:4595504‑M2/116‑1 (MQ=255)

                                                  agattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGcc                                                                                                  <  1:4956378‑M2/117‑1 (MQ=255)

                                                        gcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCAt                                                                                            >  2:2605586‑M2/29‑151 (MQ=255)

                                                         cgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATc                                                                                           <  2:6183791‑M2/124‑1 (MQ=255)

                                                         cgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATc                                                                                           <  2:6371316‑M2/124‑1 (MQ=255)

                                                          gaatatttgtacagcggatgtaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGTGACTTTCAGGCTGCTGACGCTGCCGGCcatag                                                                                          <  2:7617801‑M2/125‑3 (MQ=255)

                                                           aatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGCCGTTCAGGCTGCTGACGCAGCCGGCCATCgg                                                                                         >  2:5777979‑M2/26‑151 (MQ=255)

                                                           aatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCgg                                                                                         >  2:3037730‑M2/26‑151 (MQ=255)

                                                           aatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCgg                                                                                         >  2:8656773‑M2/26‑151 (MQ=255)

                                                            atatttgtccagcggatggactggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGACCAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGc                                                                                        <  2:485363‑M2/127‑1 (MQ=255)

                                                             tatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCa                                                                                       >  2:4402337‑M2/24‑151 (MQ=255)

                                                              atttgtacagcggatggaatggCGCGGGGCCGCGCCC‑CGGCGGCCCCGCACGCGCTCACACCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCCGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCAACGGCaa                                                                                      >  1:10424931‑M2/23‑151 (MQ=255)

                                                                  gtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTg                                                                                  >  2:7977191‑M2/19‑151 (MQ=255)

                                                                    acagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACGCCCGCGGGGTGCGCGGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCCTCGGCAACCTGGc                                                                                >  2:6606647‑M2/17‑151 (MQ=255)

                                                                        cggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAgg                                                                            >  1:7405267‑M2/13‑151 (MQ=255)

                                                                        cggatggaatggCGCGGGG‑CCGCGACGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCAGGCGGAGGATCTCGAACAGGCAGACGCCGGTCGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCAACGGCAACCTCGCGAgg                                                                            >  2:5560742‑M2/13‑151 (MQ=255)

                                                                          gatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGaa                                                                          <  2:8940053‑M2/141‑1 (MQ=255)

                                                                           atggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCGGCCGGCCATCGGCAACCTGGCGAGGAAg                                                                         >  2:7400485‑M2/10‑151 (MQ=255)

                                                                               aatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGc                                                                     <  1:7315761‑M2/146‑1 (MQ=255)

                                                                                    cgcgGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGc                                                                <  1:10177151/151‑1 (MQ=255)

                                                                                     gcgGGGCCGCG‑CCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCt                                                               >  1:3734032/1‑151 (MQ=255)

                                                                                     gcgGGGCCGCG‑CCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGAGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCt                                                               >  1:1946067/1‑151 (MQ=255)

                                                                                           gccgcgccGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCgg                                                          <  1:2414796/145‑1 (MQ=255)

                                                                                           gccgcgccGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCgg                                                          <  2:4729054/145‑1 (MQ=255)

                                                                                           gccgcgccGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCgg                                                          >  2:7106081/7‑151 (MQ=255)

                                                                                            ccgcgccGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGATCGCggg                                                         >  1:8307021/6‑151 (MQ=255)

                                                                                             cgcgccGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGt                                                        <  2:10886881/147‑1 (MQ=255)

                                                                                             cgcgccGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGt                                                        <  1:2075177/147‑1 (MQ=255)

                                                                                              gcgccGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTc                                                       <  2:3223929/148‑1 (MQ=255)

                                                                                               cgccGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCa                                                      <  2:10427795/149‑1 (MQ=255)

                                                                                                 ccGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGc                                                    <  2:975521/151‑1 (MQ=255)

                                                                                                  cgcggcggCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGcc                                                   >  2:2396014/7‑151 (MQ=255)

                                                                                                    cggcggCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCgg                                                 <  2:1526337/147‑1 (MQ=255)

                                                                                                    cggcggCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCgg                                                 >  1:1549299/5‑151 (MQ=255)

                                                                                                     ggcggCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGTATCTCGAACCGGCCGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCGGGCCATCGGAAACCTGGCGAGGAAGTCGAAGGGCTGGCGGGTCAGCCGgc                                                <  2:8558205/148‑1 (MQ=255)

                                                                                                     ggcggCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCGCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGACGTCGCAGTGCTCGCGGGTCAGCCGgc                                                >  1:5146923/4‑151 (MQ=255)

                                                                                                        ggCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCa                                             <  2:8783946/151‑1 (MQ=255)

                                                                                                         gCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCAt                                            <  2:1116898/151‑1 (MQ=255)

                                                                                                         gCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCAt                                            <  2:9253952/151‑1 (MQ=255)

                                                                                                         gCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCAt                                            <  2:9059359/151‑1 (MQ=255)

                                                                                                           cccGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGc                                          >  2:1133624/1‑151 (MQ=255)

                                                                                                           cccGCACGCGCACACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGc                                          >  1:6963703/1‑151 (MQ=255)

                                                                                                             cGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGccc                                        >  1:578892/1‑151 (MQ=255)

                                                                                                               cACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCtt                                      <  2:9461091/151‑1 (MQ=255)

                                                                                                                  gcgcTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGcc                                   <  1:4682087/151‑1 (MQ=255)

                                                                                                                   cgcTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGCCt                                  >  1:1357568/1‑151 (MQ=255)

                                                                                                                   cgcTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGCCt                                  <  2:6540880/151‑1 (MQ=255)

                                                                                                                      tCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGCCTTcc                               <  1:9895619/151‑1 (MQ=255)

                                                                                                                       cACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGCGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGCCTTCcg                              >  2:7262147/1‑151 (MQ=255)

                                                                                                                        aCTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGCCTTCcgc                             >  1:11066243/1‑151 (MQ=255)

                                                                                                                        aCTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGCCTTCcgc                             <  2:11117445/151‑1 (MQ=255)

                                                                                                                            cAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGCCTTCCGCGccc                         >  2:10265555/1‑151 (MQ=255)

                                                                                                                            cAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGCCTTCCGCGccc                         <  2:4056720/151‑1 (MQ=255)

                                                                                                                              gCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGCCTTCCGCGCCCAt                       <  2:5217313/151‑1 (MQ=255)

                                                                                                                                ggggTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGCCTTCCGCGCCCATCa                     <  1:3346804/151‑1 (MQ=255)

                                                                                                                                ggggTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGCCTTCCGCGCCCATCa                     >  1:2827427/1‑151 (MQ=255)

                                                                                                                                 gggTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGACGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGCCTTCCGCGCCCATCAc                    >  2:8231417/1‑151 (MQ=255)

                                                                                                                                  ggTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGCCTTCCGCGCCCATCAcc                   >  2:1203283/1‑151 (MQ=255)

                                                                                                                                    tGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGCCTTCCGCGCCCATCACCAg                 >  1:8553226/1‑151 (MQ=255)

                                                                                                                                      cgcTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGCCTTCCGCGCCCATCACCAGCa               >  1:1822969/1‑151 (MQ=255)

                                                                                                                                       gcTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGCCTTCCGCGCCCATCACCAGCAc              >  1:6031657/1‑151 (MQ=255)

                                                                                                                                       gcTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGCCTTCCGCGCCCATCACCAGCAc              >  1:9526719/1‑151 (MQ=255)

                                                                                                                                          ggCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGCCTTCCGCGCCCATCACCAGCACGGt           <  2:3897979/151‑1 (MQ=255)

                                                                                                                                           gCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGCCTTCCGCGCCCATCACCAGCACGGTc          <  1:1725832/151‑1 (MQ=255)

                                                                                                                                           gCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGCCTTCCGCGCCCATCACCAGCACGGTc          <  2:2851880/151‑1 (MQ=255)

                                                                                                                                             ggaggaTCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGCCTTCCGCGCCCATCACCAGCACGGTCgg        >  1:4330803/1‑151 (MQ=255)

                                                                                                                                              gaggaTCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGCCTTCCGCGCCCATCACCAGCACGGTCGGc       >  2:6853601/1‑151 (MQ=255)

                                                                                                                                                ggaTCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGCCTTCCGCGCCCATCACCAGCACGGTCGGccc     >  2:601233/1‑151 (MQ=255)

                                                                                                                                                 gaTCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGACGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGCCTTCCGCGCCCATCACCAGCACGGTCGGcccc    >  1:3935532/1‑151 (MQ=255)

                                                                                                                                                 gaTCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGCCTTCCGCGCCCATCACCAGCACGGTCGGcccc    <  1:781714/151‑1 (MQ=255)

GCGGCAAGGGGCGACATTCTAGAGAAGGGCAACCGGCGAAGCAAGGAAGATTGGCGAATATTTGTACAGCGGATGGAATGGGCGCGCGGGGCCCCCCCCGGGGGGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTTGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGCCTTCCGCGCCCATCACCAGCACGGTCGGCCCCGT  >  NC_008463/5809215‑5809512

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`