Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 5,866,730 T→G A733A (GCA→GCC)  PA14_RS26890 ← ATP‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084635,866,7300TG99.6% 1993.3 / NA 527A733A (GCA→GCC) PA14_RS26890ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base G (315/210);  minor base C (2/0);  total (317/210)
Fisher's exact test for biased strand distribution p-value = 5.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 527 total aligned reads displayed.

CGGATGAGCGGATTATTGGCGGCGGATGTAAGACGCCGTTGAACGCGCGGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCTGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAGCGCCACCAGGCCCATGGAGTGGCAGCTTCCGGACAGGCGATGG  >  NC_008463/5866582‑5866877
                                                                                                                                                    |                                                                                                                                                   
cGGATGAGCGGATTATTGGCGGCGGATGTAAGACGCCGTTGAACGCGCGGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGgc                                                                                                                                                   >  1:3521554/1‑151 (MQ=255)
 ggATGAGCGGATTATTGGCGGCGGATGTAAGACGCCGTTGAACGCGCGGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGgcg                                                                                                                                                  >  2:9370539/1‑151 (MQ=255)
 ggATGAGCGGATTATTGGCGGCGGATGTAAGACGCCGTTGAACGCGCGGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGgcg                                                                                                                                                  >  1:5291427/1‑151 (MQ=255)
 ggATGAGCGGATTATTGGCGGCGGATGTAAGACGCCGTTGAACGCGCGGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGgcg                                                                                                                                                  >  2:4137453/1‑151 (MQ=255)
 ggATGAGCGGATTATTGGCGGCGGATGTAAGACGCCGTTGAACGCGCGGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTACGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGgcg                                                                                                                                                  <  2:5479470/151‑1 (MQ=255)
  gATGAGCGGATTATTGGCGGCGGATGTAAGACGCCGTTGAACGCGCGGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGgcgc                                                                                                                                                 <  1:4544261/151‑1 (MQ=255)
    tGAGCGGATTATTGGCGGCGGATGTAAGACGCCGTTGAACGCGCGGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCg                                                                                                                                               >  1:9431418/1‑151 (MQ=255)
    tGAGCGGATTATTGGCGGCGGATGTAAGACGCCGTTGAACGCGCGGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCg                                                                                                                                               <  1:5600238/151‑1 (MQ=255)
      aGCGGATTATTGGCGGCGGATGTAAGACGCCGTTGAACGCGCGGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTc                                                                                                                                             <  2:1000047/151‑1 (MQ=255)
        cGGATTATTGGCGGCGGATGTAAGACGCCGTTGAACGCGCGGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTcgc                                                                                                                                           >  1:9679080/1‑151 (MQ=255)
        cGGATTATTGGCGGCGGATGTAAGACGCCGTTGAACGCGCGGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTcgc                                                                                                                                           >  1:3030835/1‑151 (MQ=255)
            ttattGGCGGCGGATGTAAGACGCCGTTGAACGCGCGGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCgcggcg                                                                                                                                       >  1:470234/1‑151 (MQ=255)
              attGGCGGCGGATGTAAGACGCCGTTGAACGCGCGGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCa                                                                                                                                     >  1:3276882/1‑151 (MQ=255)
              attGGCGGCGGATGTAAGACGCCGTTGAACGCGCGGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCa                                                                                                                                     <  2:747067/151‑1 (MQ=255)
                    ggcggATGTAAGACGCCGTTGAACGCGCGGATCGAAGATGTAAAAAGCTGTTAGGATGGTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGTAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCtcc                                                                                                                               >  2:10116655/1‑149 (MQ=255)
                    ggcggATGTAAGACGCCGTTGAACGCGCGGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTAc                                                                                                                               >  2:1680282/1‑151 (MQ=255)
                     gcggATGTAAGACGCCGTTGAACGCGCGGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTAcc                                                                                                                              >  1:10410047/1‑151 (MQ=255)
                      cggATGTAAGACGCCGTTGAACGCGCGGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTAccc                                                                                                                             >  2:1255684/1‑151 (MQ=255)
                         atttAAGACTCCGTTGATCGCGCTGATCGAAGATGTTAAAAGCTGTAAGTAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGTGTCGGCTTCGCCGAGGAAGCCGGCGCGGAGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAgt                                                                                                                          <  2:1587670/148‑2 (MQ=255)
                         aTGTAAGACGCCGTTGAACGCGCGGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAgg                                                                                                                          >  2:1391133/1‑151 (MQ=255)
                          tGTAAGACGCCGTTGAACGCGCGGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGc                                                                                                                         <  2:9879633/151‑1 (MQ=255)
                          tGTAAGACGCCGTTGAACGCGCGGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGc                                                                                                                         >  1:1592759/1‑151 (MQ=255)
                               gACGCCGTTGAACGCGCGGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTgcg                                                                                                                    >  2:205544/1‑151 (MQ=255)
                                aCGCCGTTGAACGCGCGGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCCGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTgcgc                                                                                                                   >  2:5738523/1‑151 (MQ=255)
                                  gCCGTTGAACGCGCGGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGACGCCGGCGCGGCGCAGCGCTTCCGCGCCGTCGCGGCGCAGGCTACCCAGGCGAGCGccc                                                                                                                 >  2:7272308/1‑151 (MQ=255)
                                  gCCGTTGAACGCGCGGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGccc                                                                                                                 >  1:8335270/1‑151 (MQ=255)
                                   ccGTTGAACGCGCGGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGcccc                                                                                                                >  1:4235042/1‑151 (MQ=255)
                                    cGTTGAACGCGCGGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCa                                                                                                               >  2:10358130/1‑151 (MQ=255)
                                     gTTGAACGCGCGGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAg                                                                                                              >  2:1058409/1‑151 (MQ=255)
                                     gTTGAACGCGCGGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAg                                                                                                              <  1:7244195/151‑1 (MQ=255)
                                         aaCGCGCGGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTc                                                                                                          >  1:625937/1‑151 (MQ=255)
                                           cgcgcgGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTcgc                                                                                                        <  1:2405640/151‑1 (MQ=255)
                                             cgcgGCACGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCAGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCgg                                                                                                      <  1:4922825/151‑1 (MQ=255)
                                               cgGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGt                                                                                                    <  1:6735274/151‑1 (MQ=255)
                                                 gATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCg                                                                                                  <  1:9228073/151‑1 (MQ=255)
                                                    cGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGATTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGAcc                                                                                               >  1:8785083/1‑151 (MQ=255)
                                                      aaGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGAcccc                                                                                             >  1:10157259/1‑151 (MQ=255)
                                                       aGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGGCGACCCCg                                                                                            >  1:1355137/1‑151 (MQ=255)
                                                          tGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGcc                                                                                         <  1:10011177/151‑1 (MQ=255)
                                                          tGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGcc                                                                                         >  2:7826331/1‑151 (MQ=255)
                                                          tGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGcc                                                                                         >  1:126076/1‑151 (MQ=255)
                                                           gTACAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTCCCCAGGCGTGCGCCCCAGGCGCGCGGGTCGACCCCGGCcg                                                                                        >  2:5615039/1‑151 (MQ=255)
                                                            tAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGGCGACCCCGGCcgc                                                                                       >  2:3793302/1‑151 (MQ=255)
                                                             aaaaaGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCcgcg                                                                                      >  2:2553159/1‑151 (MQ=255)
                                                             aaaaaGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCcgcg                                                                                      >  1:3774172/1‑151 (MQ=255)
                                                               aaaGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGcc                                                                                    >  2:510636/1‑151 (MQ=255)
                                                                 aGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCa                                                                                  <  1:9100315/151‑1 (MQ=255)
                                                                  gCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAg                                                                                 <  2:6840219/151‑1 (MQ=255)
                                                                      tAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGACGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCTGGCTACCCAGGCGTGCGCCCCAGGCGCGCGGGTCGACCCCGGCCGCGCCCTGGGcc                                                                             >  2:8616936/1‑151 (MQ=255)
                                                                       aaGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCt                                                                            >  2:5180293/1‑151 (MQ=255)
                                                                        aGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTc                                                                           <  1:10805102/151‑1 (MQ=255)
                                                                            aGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCgcgc                                                                       >  2:11105748/1‑151 (MQ=255)
                                                                             gttgttCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCt                                                                      <  1:5549117/151‑1 (MQ=255)
                                                                              ttgttCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCgcgcgc                                                                     >  2:2805535/1‑149 (MQ=255)
                                                                              ttgttCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTc                                                                     <  1:3687098/151‑1 (MQ=255)
                                                                              ttgttCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTc                                                                     >  1:6636485/1‑151 (MQ=255)
                                                                               tgttCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTcc                                                                    >  2:10423296/1‑151 (MQ=255)
                                                                                gttCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCa                                                                   >  2:7580655/1‑151 (MQ=255)
                                                                                gttCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCa                                                                   >  1:10723735/1‑151 (MQ=255)
                                                                                      cgcgccgcTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCTCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGc                                                             <  2:9031382/151‑1 (MQ=255)
                                                                                      cgcgccgcTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCCGGGCCTCGCGCTCCAGCTCGc                                                             >  2:1090182/1‑151 (MQ=255)
                                                                                      cgcgccgcTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCAGGCGCGGCGCAGGGCTTCGGCGCAGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCAAGGGCCTCGCGCTCCAGCTCGc                                                             >  2:6246553/1‑151 (MQ=255)
                                                                                       gcgccgcTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGcc                                                            >  2:8282873/1‑151 (MQ=255)
                                                                                       gcgccgcTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGcc                                                            >  1:4808353/1‑151 (MQ=255)
                                                                                          ccgcTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAg                                                         >  1:7629528/1‑151 (MQ=255)
                                                                                            gcTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGc                                                       >  2:3590761/1‑151 (MQ=255)
                                                                                             cTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCTGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGcc                                                      >  2:4849959/1‑151 (MQ=255)
                                                                                              tCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCg                                                     <  2:8766245/151‑1 (MQ=255)
                                                                                                    ggCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCg                                               >  1:4310642/1‑151 (MQ=255)
                                                                                                    ggCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGAGCCGTCGCGGCGCAGGATACCCAGGCGTGCGCCCCAGGCTCGCGTGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCg                                               >  2:4380220/1‑151 (MQ=255)
                                                                                                     gCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGa                                              >  2:6072914/1‑151 (MQ=255)
                                                                                                       cGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAgc                                            >  2:10781020/1‑151 (MQ=255)
                                                                                                        gCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGTCTTCGGCGCGGTCGCGGCGCAGGAGACCCAGGCGAGCGCGCCGGGCTCGCGGGTCGACCCCGGCCGCGGCAAGGGCATCGCGCTCCAGCTCGCCCAGGACGGCGCCGAgcg                                           >  2:3927630/1‑151 (MQ=255)
                                                                                                        gCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAgcg                                           <  2:1969001/151‑1 (MQ=255)
                                                                                                          tGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAGCGcc                                         <  2:977843/151‑1 (MQ=255)
                                                                                                           gAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAGCGcca                                        >  2:9089835/1‑151 (MQ=255)
                                                                                                           gAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAGCGcca                                        >  2:6619761/1‑151 (MQ=255)
                                                                                                              tCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCCGGCGCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAGCGccacca                                     <  2:3123970/151‑1 (MQ=255)
                                                                                                              tCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAGCGccacca                                     <  1:1113693/151‑1 (MQ=255)
                                                                                                                   ttCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAGCGCCACCAGGccc                                <  1:10547507/151‑1 (MQ=255)
                                                                                                                   atCGCCGAGGAAGCCGGCGCGGCGCAGGGCCTCGGCGCCGTCGCGGCGCAGGCTCCCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCCGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAGCGCCACCCGGccc                                <  2:11172348/150‑1 (MQ=255)
                                                                                                                     cGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAGCGCCACCAGGCCCAt                              >  2:5004256/1‑151 (MQ=255)
                                                                                                                      gCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAGCGCCACCAGGCCCATg                             >  1:2660102/1‑151 (MQ=255)
                                                                                                                          cggACGCCGGCGCGGCGCCGGGCGTCGGCGCCGACGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAGCGCCACCAGGCCCATGGAGt                         <  2:3442057/150‑1 (MQ=255)
                                                                                                                            gAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAGCGCCACCAGGCCCATGGAGTgg                       <  1:4047228/151‑1 (MQ=255)
                                                                                                                              aGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAGCGCCACCAGGCCCATGGAGTGGCa                     <  1:2201789/151‑1 (MQ=255)
                                                                                                                               gCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAGCGCCACCAGGCCCATGGAGTGGCAg                    <  2:7996409/151‑1 (MQ=255)
                                                                                                                                  ggcgcggcgcAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAGCGCCACCAGGCCCATGGAGTGGCAGcgt                 >  1:8161477/1‑149 (MQ=255)
                                                                                                                                  ggcgcggcgcAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAGCGCCACCAGGCCCATGGAGTGGCAGCtg                 >  1:740545/1‑150 (MQ=255)
                                                                                                                                   gcgcgtcgcAGTGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCACAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGTCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAGCGCCACCAGGCCCATGGAGTGGCAGCTTc                <  2:3300662/151‑1 (MQ=255)
                                                                                                                                    cgcggcgcAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAGCGCCACCAGGCCCATGGAGTGGCAGCTTcc               >  1:5972386/1‑151 (MQ=255)
                                                                                                                                      cggcgcAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAGCGCCACCAGGCCCATGGAGTGGCAGCTTCCgg             >  2:5974881/1‑151 (MQ=255)
                                                                                                                                         cgcAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAGCGCCACCAGGCCCATGGAGTGGCAGCTTCCGGACa          <  1:10891571/151‑1 (MQ=255)
                                                                                                                                           cAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAGCGCCACCAGGCCCATGGAGTGGCAGCTTCCGGACAgg        >  1:187108/1‑151 (MQ=255)
                                                                                                                                           cAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAGCGCCACCAGGCCCATGGAGTGGCAGCTTCCGGACAgg        <  1:7940686/151‑1 (MQ=255)
                                                                                                                                            aGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAGCGCCACCAGGCCCATGGAGTGGCAGCTTCCGGACAggc       <  1:7089132/151‑1 (MQ=255)
                                                                                                                                            aGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAGCGCCACCAGGCCCATGGAGTGGCAGCTTCCGGACAggc       >  1:64201/1‑151 (MQ=255)
                                                                                                                                             gggCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAGCGCCACCAGGCCCATGGAGTGGCAGCTTCCGGACAggcg      >  2:8860553/1‑151 (MQ=255)
                                                                                                                                             gggCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAGCGCCACCAGGCCCATGGAGTGGCAGCTTCCGGACAggcg      <  2:7050550/151‑1 (MQ=255)
                                                                                                                                                cTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAGCGCCACCAGGCCCATGGAGTGGCAGCTTCCGGACAggcgatg   >  2:4648244/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CGGATGAGCGGATTATTGGCGGCGGATGTAAGACGCCGTTGAACGCGCGGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCTGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAGCGCCACCAGGCCCATGGAGTGGCAGCTTCCGGACAGGCGATGG  >  NC_008463/5866582‑5866877

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑