BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_008463	757,675	A→G	T671A (ACC→GCC)	fusA →	elongation factor G

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_008463	757,675	0	A	G	99.7%	1301.5 / NA	340	T671A (ACC→GCC)	fusA	elongation factor G
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (113/226); minor base T (0/1); total (113/227)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 347 total aligned reads displayed.

GGTAGAGGTGGTGACTCCTGAGGACTACATGGGTGACGTGATGGGCGACCTGAACCGTCGTCGTGGTCTGATTCAGGGGATGGAGGACACGGTTTCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCAGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACAAGGTTGATTCAGCCCTTTAAGTAAGAGGTTTACTGTCGTGGCT  >  NC_008463/757527‑757821

ggTAGAGGTGGTGACTCCTGAGGACTACATGGGTGACGTGATGGGCGACCTGAACCGTCGTCGTGGTCTGATTCAGGGGATGGAGGACACGGTTTCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGcggcc                                                                                                                                                  >  2:984086/1‑151 (MQ=255)

 gTAGAGGTGGTGACTCCTGAGGACTACATGGGTGACGTGATGGGCGACCTGAACCGTCGTCGTGGTCTGATTCAGGGGATGGAGGACACGGTTTCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGcggccg                                                                                                                                                 <  1:5992217/151‑1 (MQ=255)

  tAGAGGTGGTGACTCCTGAGGACTACATGGGTGACGTGATGGGCGACCTGAACCGTCGTCGTGGTCTGATTCAGGGGATGGAGGACACGGTTTCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGcggccga                                                                                                                                                >  2:5437631/1‑151 (MQ=255)

    gagGTGGTGACTCCAGAGGACTACATGGGTGACGTGATGGGCGACCTGAACCGTCGTCGTGGTCTGATTCAGGGGATGGAGGACACGGTTTCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGGCCGACg                                                                                                                                              <  1:1035755/151‑1 (MQ=255)

     agGTGGTGACTCCTGAGGACTACATGGGTGACGTGATGGGCGACCTGAACCGTCGTCGTGGTCTGATTCAGGGGATGGAGGACACGGTTTCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGGCCGACGt                                                                                                                                             <  1:8718196/151‑1 (MQ=255)

      ggtggtGACTCCTGAGGACTACATGGGTGACGTGATGGGCGACCTGAACCGTCGTCGTGGTCTGATTCAGGGGATGGAGGACACGGTTTCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGGCCGACGTg                                                                                                                                            <  2:3063002/151‑1 (MQ=255)

        tggtgACTCCTGAGGACTACATGGGTGACGTGATGGGCGACCTGAACCGTCGTCGTGGTCTGATTCAGGGGATGGAGGACACGGTTTCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGGCCGACGTGCg                                                                                                                                          <  1:485282/151‑1 (MQ=255)

         ggtgACTCCTGAGGACTACATGGGTGACGTGATGGGCGACCTGAACCGTCGTCGTGGTCTGATTCAGGGGATGGAGGACACGGTTTCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGGCCGACGTGCGt                                                                                                                                         <  2:6423175/151‑1 (MQ=255)

               tCCTGAGGACTACATGGGTGACGTGATGGGCGACCTGAACCGTCGTCGTGGTCTGATTCAGGGGATGGAGGACACGGTTTCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGGCCGACGTGCGTTCCATg                                                                                                                                   >  1:5831981/1‑151 (MQ=255)

                 cTGAGGACTACATGGGTGACGTGATGGGCGACCTGAACCGTCGTCGTGGTCTGATTCAGGGGATGGAGGACACGGTTTCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGGCCGACGTGCGTTCCATGTc                                                                                                                                 <  1:6227242/151‑1 (MQ=255)

                   gAGGACTACATGGGTGACGTGATGGGCGACCTGAACCGTCGTCGTGGTCTGATTCAGGGGATGGAGGACACGGTTTCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGGCCGACGTGCGTTCCATGTccc                                                                                                                               <  1:8033443/151‑1 (MQ=255)

                    atgACTACATGGGTGACGTGATGGGCGACCTGAACCGTCGTCGTGGTCTGATTCAGGGGATGGAGGACACGGTTTCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGGCCGACGTGCGTTCCATGTCCCa                                                                                                                              >  1:4355712/3‑151 (MQ=255)

                     ggACTACATGGGTGACGTGATGGGCGACCTGAACCGTCGTCGTGGTCTGATTCAGGGGATGGAGGACACGGTTTCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGGCCGACGTGCGTTCCATGTccctg                                                                                                                             <  2:8884176/151‑3 (MQ=255)

                       aCTACATGGGTGACGTGATGGGCGACCTGAACCGTCGTCGTGGTCTGATTCAGGGGATGGAGGACACGGTTTCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGGCCGACGTGCGTTCCATGTCCCAggg                                                                                                                           <  2:7998727/151‑1 (MQ=255)

                         gaCATGGGTGACGTGATGGGCGACCTGAACCGTCGTCGTGGTCTGATTCAGGGGATGGAGGACACGGTTTCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGGCCGACGTGCGTTCCATGTCCCAGGGTc                                                                                                                         <  1:10852130/150‑1 (MQ=255)

                            aTGGGTGACGTGATGGGCGACCTGAACCGTCGTCGTGGTCTGATTCAGGGGATGGAGGACACGGTTTCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGGCCGACGTGCGTTCCATGTCCCAGGGTcgcg                                                                                                                      <  2:1684239/151‑1 (MQ=255)

                            aTGGGTGACGTGATGGGCGACCTGAACCGTCGTCGTGGTCTGATTCAGGGGATGGAGGACACGGTTTCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGGCCGACGTGCGTTCCATGTCCCAGGGTcgcg                                                                                                                      <  1:5443300/151‑1 (MQ=255)

                            aTGGGTGACGTGATGGGCGACCTGAACCGTCGTCGTGGTCTGATTCAGGGGATGGAGGACACGGTTTCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGGCCGACGTGCGTTCCATGTCCCAGGGTcgcg                                                                                                                      <  2:3656030/151‑1 (MQ=255)

                                gTGACGTGATGGGCGACCTGAACCGTCGTCGTGGTCTGATTCAGGGGATGGAGGACACGGTTTCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGGCCGACGTGCGTTCCATGTCCCAGGGTCGCGCGAg                                                                                                                  <  1:8421341/151‑1 (MQ=255)

                                 tGACGTGATGGGCGACCTGAACCGTCGTCGTGGTCTGATTCAGGGGATGGAGGACACGGTTTCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGGCCGACGTGCGTTCCATGTCCCAGGGTCGCGCGAGc                                                                                                                 <  2:185607/151‑1 (MQ=255)

                                 tGACGTGATGGGCGACCTGAACCGGCGTCGTGGTCTGATTCAGGGGATGGAGGACACGGTTTCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGGCCGACGTGCGTTCCATGTCCCAGGGTCGCGCGAGc                                                                                                                 >  2:10523062/1‑151 (MQ=255)

                                  gACGTGATGGGCGACCTGAACCGTCGTCGTGGTCTGATTCAGGGGATGGAGGACACGGTTTCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGGCCGACGTGCGTTCCATGTCCCAGGGTCGCGCGAGCt                                                                                                                <  2:1480794/151‑1 (MQ=255)

                                  gACGTGATGGGCGACCTGAACCGTCGTCGTGGTCTGATTCAGGGGATGGAGGACACGGTTTCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGGCCGACGTGCGTTCCATGTCCCAGGGTCGCGCGAGCt                                                                                                                >  2:9652543/1‑151 (MQ=255)

                                   aCGTGATGGGCGACCTGAACCGTCGTCGTGGTCTGATTCAGGGGATGGAGGACACGGTTTCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGGCCGACGTGCGTTCCATGTCCCAGGGTCGCGCGAGCTa                                                                                                               <  2:8434396/151‑1 (MQ=255)

                                    cGTGATGGGCGACCTGAACCGTCGTCGTGGTCTGATTCAGGGGATGGAGGACACGGTTTCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGGCCGACGTGCGTTCCATGTCCCAGGGTCGCGCGAGCTAc                                                                                                              <  1:7256522/151‑1 (MQ=255)

                                      tGATGGGCGACCTGAACCGTCGTCGTGGTCTGATTCAGGGGATGGAGGACACGGTTTCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGGCCGACGTGCGTTCCATGTCCCAGGGTCGCGCGAGCTACTc                                                                                                            <  1:2947985/151‑1 (MQ=255)

                                         tGGGCGACCTGAACCGTCGTCGTGGTCTGATTCAGGGGATGGAGGACACGGTTTCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGGCCGACGTGCGTTCCATGTCCCAGGGTCGCGCGAGCTACTCCAt                                                                                                         >  2:10943245/1‑151 (MQ=255)

                                          gggCGACCTGAACCGTCGTCGTGGTCTGATTCAGGGGATGGAGGACACGGTTTCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGGCCGACGTGCGTTCCATGTCCCAGGGTCGCGCGAGCTACTCCATg                                                                                                        <  1:8282549/151‑1 (MQ=255)

                                           ggCGACCTGAACCGTCGTCGTGGTCTGATTCAGGGGATGGAGGACACGGTTTCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGGCCGACGTGCGTTCCATGTCCCAGGGTCGCGCGAGCTACTCCATgg                                                                                                       <  2:2799185/151‑1 (MQ=255)

                                                 cTGAACCGTCGTCGTGGTCTGATTCAGGGGATGGAGGACACGGTTTCCGGTAATGTTATTCGTGCCGAGGTTCCGCTGGGTGATATGTTCGGTTATGCGGCCGACGCGCGTTCCATGTCCCAGGGTCGCGCGAGCTACTCCATGGAATtct                                                                                                 <  2:330142/151‑1 (MQ=255)

                                                  tGAACCGTCGTCGTGGTCTGATTCAGGGGATGGAGGACACGGTTTCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGGCCGACGTGCGTTCCATGTCCCAGGGTCGCGCGAGCTACTCCATGGAATtctc                                                                                                <  2:8632994/151‑1 (MQ=255)

                                                        gtcgtcgtGGTCTGATTCAGGGGATGGAGGACACGGTTTCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGGCCGACGTGCGTTCCATGTCCCAGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATa                                                                                          >  1:7150752/1‑151 (MQ=255)

                                                         tcgtcgtGGTCTGATTCAGGGGATGGAGGACACGGTTTCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGGCCGACGTGCGTTCCATGTCCCAGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATAc                                                                                         <  2:4943827/151‑1 (MQ=255)

                                                            tcgtGGTCTGATTCAGGGGATGGAGGACACGGTTTCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGGCCGACGTGCGTTCCATGTCCCAGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGcc                                                                                      <  2:2417699/151‑1 (MQ=255)

                                                             cgtGGTCTGATTCAGGGGATGGAGGACACGGTTTCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGGCCGACGTGCGTTCCATGTCCCAGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCg                                                                                     >  1:10730296/1‑151 (MQ=255)

                                                              gtGGTCTGATTCAGGGGATGGAGGACACGGTTTCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGGCCGACGTGCGTTCCATGTCCCAGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGa                                                                                    >  1:7563895/1‑151 (MQ=255)

                                                                  tCTGATTCAGGGGATGGAGGACACGGTTTCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGGCCGACGTGCGTTCCATGTCCCAGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCt                                                                                <  1:4601913/151‑1 (MQ=255)

                                                                   cTGATTCAGGGGATGGAGGACACGGTTTCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGGCCGACGTGCGTTCCATGTCCCAGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTc                                                                               <  1:6470862/151‑1 (MQ=255)

                                                                   cTGATTCAGGGGATGGAGGACACGGTTTCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGGCCGACGTGCGTTCCATGTCCCAGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTc                                                                               <  2:11168273/151‑1 (MQ=255)

                                                                    tGATTCAGGGGATGGAGGACACGGTTTCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGGCCGACGTGCGTTCCATGTCCCAGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTcc                                                                              <  1:923349/151‑1 (MQ=255)

                                                                    tGATTCAGGGGATGGAGGACACGGTTTCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGGCCGACGTGCGTTCCATGTCCCAGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTcc                                                                              >  2:5885154/1‑151 (MQ=255)

                                                                     gATTCAGGGGATGGAGGACACGGTTTCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGGCCGACGTGCGTTCCATGTCCCAGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCg                                                                             >  2:6774965/1‑151 (MQ=255)

                                                                      aTTCAGGGGATGGAGGACACGGTTTCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGGCCGACGTGCGTTCCATGTCCCAGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGt                                                                            <  1:3154636/151‑1 (MQ=255)

                                                                       ttCAGGGGATGGAGGACCCGGTTTGCGGTAAGGTTAGTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTCTGCGGCCGACGTGCGTTCCATGTCCCAGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTc                                                                           <  2:10445590/151‑1 (MQ=255)

                                                                       ttCAGGGGATGGAGGACACGGTTTCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGGCCGACGTGCGTTCCATGTCCCAGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTc                                                                           <  2:6899070/151‑1 (MQ=255)

                                                                       ttCAGGGGATGGAGGACACGGTTTCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGGCCGACGTGCGTTCCATGTCCCAGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTc                                                                           <  1:4997101/151‑1 (MQ=255)

                                                                           ggggATGGAGGACACGGTTTCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGGCCGACGTGCGTTCCATGTCCCAGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAAc                                                                       <  2:4603498/151‑1 (MQ=255)

                                                                             ggATGGAGGACACGGTTTCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGGCCGACGTGCGTTCCATGTCCCAGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACAt                                                                     >  2:2045571/1‑151 (MQ=255)

                                                                             ggATGGAGGACACGGTTTCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGGCCGACGTGCGTTCCATGTCCCAGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACAt                                                                     >  2:6211833/1‑151 (MQ=255)

                                                                               aTGGAGGACACGGTTTCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGGCCGACGTGCGTTCCATGTCCCAGGGTCGCGCGAGCTCCTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACAtcg                                                                   >  2:10241000/1‑151 (MQ=255)

                                                                                 ggaggaCACGGTTTCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGGCCGACGTGCGTTCCATGTCCCAGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACAtcgtc                                                                 >  1:8909789/1‑151 (MQ=255)

                                                                                  gaggaCACGGTTTCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGGCCGACGTGCGTTCCATGTCCCAGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACAtcgtcg                                                                <  2:7568355/151‑1 (MQ=255)

                                                                                   aggaCACGGTTTCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGGCCGACGTGCGTTCCATGTCCCAGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGa                                                               >  1:188289/1‑151 (MQ=255)

                                                                                    ggaCACGGTTTCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGGCCGACGTGCGTTCCATGTCCCAGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGaa                                                              <  2:9750711/151‑1 (MQ=255)

                                                                                     gaCACGGTTTCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGGCCGACGTGCGTTCCATGTCCCAGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAg                                                             <  1:6880326/151‑1 (MQ=255)

                                                                                      acacGGTTTCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGGCCGACGTGCGTTCCATGTCCCAGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAgc                                                            <  1:2465773/151‑1 (MQ=255)

                                                                                       cacGGTTTCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGGCCGACGTGCGTTCCATGTCCCAGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAgcg                                                           >  1:9699558/1‑151 (MQ=255)

                                                                                        acGGTTTCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGGCCGACGTGCGTTCCATGTCCCAGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAgcgc                                                          <  2:10110246/151‑1 (MQ=255)

                                                                                         cGGTTTCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGGCCGACGTGCGTTCCATGTCCCAGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCt                                                         <  2:2440022/151‑1 (MQ=255)

                                                                                            tttCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGGCCGACGTGCGTTCCATGTCCCAGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGt                                                      >  1:10026784/1‑151 (MQ=255)

                                                                                             ttCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGGCCGACGTGCGTTCCATGTCCCAGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCt                                                                                <  2:4120461/124‑1 (MQ=255)

                                                                                             ttCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGGCCGACGTGCGTTCCATGTCCCAGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGtt                                                     <  2:2192094/151‑1 (MQ=255)

                                                                                             ttCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGGCCGACGTGCGTTCCATGTCCCAGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGtt                                                     <  1:1676231/151‑1 (MQ=255)

                                                                                                cGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGGCCGACGTGCGTTCCATGTCCCAGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTaaa                                                  <  1:6689238/151‑1 (MQ=255)

                                                                                                cGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGGCCGACGTGCGTTCCATGTCCCAGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTaaa                                                  <  2:7751316/151‑1 (MQ=255)

                                                                                                 ggTAAGGTTTTTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGGCCGACGTGCGTTCCATGTCCCAGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTaaaa                                                 <  1:10279841/151‑1 (MQ=255)

                                                                                                     aGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGGCCGACGTGCGTTCCATGTCCCAGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACa                                             >  2:1224488/1‑151 (MQ=255)

                                                                                                        ttATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGGCCGACGTGCGTTCCATGTCCCAGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACAAgg                                          <  1:9447799/151‑1 (MQ=255)

                                                                                                        ttATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGGCCGACGTGCGTTCCATGTCCCAGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACAAgg                                          >  1:2575387/1‑151 (MQ=255)

                                                                                                          aTTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGGCCGACGTGCGTTCCATGTCCCAGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACAAGGtt                                        >  1:4203777/1‑151 (MQ=255)

                                                                                                          aTTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGGCCGACGTGCGTTCCATGTCCCAGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACAAGGtt                                        <  2:5197314/151‑1 (MQ=255)

                                                                                                           ttCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGGCCGACGTGCGTTCCATGTCCCAGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACAAGGTTg                                       <  2:8349672/151‑1 (MQ=255)

                                                                                                              gTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGGCCGACGTGCGTTCCATGACCCAGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACAAGGTTTAtt                                    >  2:7410034/1‑151 (MQ=255)

                                                                                                               tGCCGAGGTTCCGCGGGGTGAGATGTTCGGTTATGCGGCCGACGTGCGTTCCATGTCCCAGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACAAGGTTGATTc                                   >  2:9902500/1‑151 (MQ=255)

                                                                                                                  cGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGGCCGACGTGCGTTCCATGTCCCAGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACAAGGTTGATTCAGc                                >  1:10173129/1‑151 (MQ=255)

                                                                                                                  cGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGGCCGACGTGCGTTCCATGACCCAGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACAAGGTTGATTCAGc                                >  1:9651045/1‑151 (MQ=255)

                                                                                                                   gAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGGCCGACGTGCGTTCCATGTCCCAGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACAAGGTTGATTCAGcc                               >  1:2343689/1‑151 (MQ=255)

                                                                                                                   gAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGGCCGACGTGCGTTCCATGTCCCAGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACAAGGTTGATTCAGcc                               <  1:6947818/151‑1 (MQ=255)

                                                                                                                    aGGTTCCGCTGGGTGAGATGTTCGGTTATGCGGCCGACGTGCGTTCCATGTCCCAGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACAAGGTTGATTCAGccc                              >  1:1542410/1‑151 (MQ=255)

                                                                                                                    aGGTTCCGCTGGGTGAGATGTTCGGTTATGCGGCCGACGTGCGTTCCATGTCCCAGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACAAGGTTGATTCAGccc                              <  2:10786408/151‑1 (MQ=255)

                                                                                                                    aGGTTCCGCTGGGTGAGATGTTCGGTTATGCGGCCGACGTGCGTTCCATGTCCCAGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACAAGGTTGATTCAGccc                              >  1:3525738/1‑151 (MQ=255)

                                                                                                                      gTTCCGCTGGGTGAGATGTTCGGTTATGCGGCCGACGTGCGTTCCATGTCCCAGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACAAGGTTGATTCAGCCCtt                            >  2:3813985/1‑151 (MQ=255)

                                                                                                                        tCCGCTGGGTGAGATGTTCGGTTATGCGGCCGACGTGCGTTCCATGTCCCAGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACAAGGTTGATTCAGCCCTTta                          <  1:1598918/151‑1 (MQ=255)

                                                                                                                           gCTGGGTGAGATGTTCGGTTATGCGGCCGACGTGCGTTCCATGTCCCAGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACAAGGTTGATTCAGCCCTTtaagt                       >  2:9441553/1‑151 (MQ=255)

                                                                                                                            cTGGGTGAGATGTTCGGTTATGCGGCCGACGTGCGTTCCATGTCCCAGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACAAGGTTGATTCAGCCCTTtaagta                      <  1:1712360/151‑1 (MQ=255)

                                                                                                                             tGGGTGAGATGTTCGGTTATGCGGCCGACGTGCGTTCCATGTCCCAGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACAAGGTTGATTCAGCCCTTtaagtaa                     <  2:3508251/151‑1 (MQ=255)

                                                                                                                             tGGGTGAGATGTTCGGTTATGCGGCCGACGTGCGTTCCATGTCCCAGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACAAGGTTGATTCAGCCCTTtaagtaa                     <  2:6170416/151‑1 (MQ=255)

                                                                                                                              gggTGAGATGTTCGGTTATGCGGCCGACGTGCGTTCCATGTCCCAGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACAAGGTTGATTCAGCCCTTtaagtaag                    >  1:3678423/1‑151 (MQ=255)

                                                                                                                               ggTGAGATGTTCGGTTATGCGGCCGACGTGCGTTCCATGTCCCAGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACAAGGTTGATTCAGCCCTTTAAGTAaga                   >  2:10425283/1‑151 (MQ=255)

                                                                                                                                gTGAGATGTTCGGTTATGCGGCCGACGTGCGTTCCATGTCCCAGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACAAGGTTGATTCAGCCCTTTAAGTAagag                  >  1:7857584/1‑151 (MQ=255)

                                                                                                                                   agaTGTTCGGTTATGCGGCCGACGTGCGTTCCATGTCCCAGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACAAGGTTGATTCAGCCCTTTAAGTAAGAGGtt               >  2:8108536/1‑151 (MQ=255)

                                                                                                                                   agaTGTTCGGTTATGCGGCCGACGTGCGTTCCATGTCCCAGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACAAGGTTGATTCAGCCCTTTAAGTAAGAGGtt               >  2:9147914/1‑151 (MQ=255)

                                                                                                                                      tGTTCGGTTATGCGGCCGACGTGCGTTCCATGTCCCAGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACAAGGTTGATTCAGCCCTTTAAGTAAGAGGTTTAc            >  2:942220/1‑151 (MQ=255)

                                                                                                                                       gTTCGGTTATGCGGCCGACGTGCGTTCCATGTCCCAGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACAAGGTTGATTCAGCCCTTTAAGTAAGAGGTTTACt           <  1:1160374/151‑1 (MQ=255)

                                                                                                                                        ttCGGTTATGCGGCCGACGTGCGTTCCATGTCCCAGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACAAGGTTGATTCAGCCCTTTAAGTAAGAGGTTTACTg          <  1:10552419/151‑1 (MQ=255)

                                                                                                                                         tCGGTTATGCGGCCGACGTGCGTTCCATGTCCCAGGGTCGCGCGAGCGACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACAAGGTTGATTCAGCCCTTTAAGTAAGAGGTTTACTGt         <  2:7188338/151‑1 (MQ=255)

                                                                                                                                           ggTTATGCGGCCGACGTGCGTTCCATGTCCCAGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACAAGGTTGATTCAGCCCTTTAAGTAAGAGGTTTACTGTCg       >  2:2347655/1‑151 (MQ=255)

                                                                                                                                           ggTTATGCGGCAGACGTGCGTTCCATGTCCCAGGGTCGCGCGAGCTACTCCATGGAATTCTCCTAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACAAGGTTGATTCAGCCCTTTAAGTAAGAGGTTTACTGTCg       <  2:3179232/151‑1 (MQ=255)

                                                                                                                                               aTGCGGCCGACGTGCGTTCCAGGTCCCAGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACAAGGTTGAGTCAGCCCTTTAAGTAAGAGGTTTACTGTCGTGGc   >  2:6990443/1‑151 (MQ=255)

                                                                                                                                                tGCGGCCGACGTGCGTTCCATGTCCCAGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACAAGGTTGATTCAGCCCTTTAAGTAAGAGGTTTACTGTCGTGGCt  <  1:5091813/151‑1 (MQ=255)

GGTAGAGGTGGTGACTCCTGAGGACTACATGGGTGACGTGATGGGCGACCTGAACCGTCGTCGTGGTCTGATTCAGGGGATGGAGGACACGGTTTCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCAGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACAAGGTTGATTCAGCCCTTTAAGTAAGAGGTTTACTGTCGTGGCT  >  NC_008463/757527‑757821

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`