Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 927,917 T→C I56T (ATC→ACC)  PA14_RS04340 → PAS domain S‑box protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_008463927,9170TC99.9% 2531.9 / NA 653I56T (ATC→ACC) PA14_RS04340PAS domain S‑box protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (320/332);  minor base G (1/0);  total (321/332)
Fisher's exact test for biased strand distribution p-value = 4.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 658 total aligned reads displayed.

AAGTTCAGGCTGAGCCGCTGGGCGGGCGCGACACTGGCGCTCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGATCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGACCCGCGACATCGACCGCGAGTTTCCCGGCGCCCGCGGCTT  >  NC_008463/927769‑928064
                                                                                                                                                    |                                                                                                                                                   
 aGTTCAGGCTGAGCCGCTGGGCGGGCGCGACACTGGCGCTCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACccg                                                                                                                                                  <  2:10706035/151‑1 (MQ=255)
   ttCAGGCTGAGCCGCTGGGCGGGCGCGACACTGGCGCTCGGACAGTTGCTGTCCGGGCTTGGCGCCTGGGTTCTGGCGCTGGTCAACGAGCAACATGCGCGGATGGCGCTGGATCGCGAGGCGGAACTGCTGGCCGAGGCGGTGACccgac                                                                                                                                                <  2:2707731/151‑3 (MQ=255)
      aGGCTGAGCCGCTGGGCGGGCGCGACACTGGCGCTCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCcgcg                                                                                                                                             >  2:9157730/1‑151 (MQ=255)
      aGGCTGAGCCGCTGGGCGGGCGCGACACTGGCGCTCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCcgcg                                                                                                                                             <  1:9426402/151‑1 (MQ=255)
        gCTGAGCCGCTGGGCGGGCGCGACACTGGCGCTCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTg                                                                                                                                           <  1:9693071/151‑1 (MQ=255)
          tGAGCCGCTGGGCGGGCGCGACACTGGCGCTCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGa                                                                                                                                         >  1:3311491/1‑151 (MQ=255)
             gCCGCTGGGCGGGCGCGACACTGGCGCTCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGACct                                                                                                                                      <  1:3879572/151‑1 (MQ=255)
               cGCTGGGCGGGCGCGACACTGGCGCTCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAActct                                                                                                                                    >  1:3634300/1‑151 (MQ=255)
                    ggcgggcgCGACCCTGGCGCTCGGACTGTTGCGGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAg                                                                                                                               <  2:1424920/151‑1 (MQ=255)
                    ggcgggcgCGACACTGGCGCTCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAg                                                                                                                               <  2:4409741/151‑1 (MQ=255)
                      cgggcgCGACACTGGCGCTCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTa                                                                                                                             <  2:964804/151‑1 (MQ=255)
                       gggcgCGACACTGGCGCTCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTAt                                                                                                                            >  1:10815602/1‑151 (MQ=255)
                             gACACTGGCGCTCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTg                                                                                                                      <  2:188558/151‑1 (MQ=255)
                                acTGGCGCTCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGt                                                                                                                   >  1:10280236/1‑151 (MQ=255)
                                acTGGCGCTCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGt                                                                                                                   <  2:8380980/151‑1 (MQ=255)
                                 cTGGCGCTCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTg                                                                                                                  <  1:9234827/151‑1 (MQ=255)
                                  tGGCGCTCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTgg                                                                                                                 <  1:2010260/151‑1 (MQ=255)
                                        tCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGACCGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTgcg                                                                                                           >  2:2765947/1‑151 (MQ=255)
                                          ggACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCACCGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGcgcg                                                                                                         <  2:8044878/151‑1 (MQ=255)
                                          ggACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGcgcg                                                                                                         <  2:4054743/151‑1 (MQ=255)
                                           gACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCgg                                                                                                        >  1:5697443/1‑151 (MQ=255)
                                           gACTGGTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCgg                                                                                                        <  2:8197145/151‑1 (MQ=255)
                                            aCTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGt                                                                                                       >  2:10287063/1‑151 (MQ=255)
                                            aCTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGt                                                                                                       >  2:3454467/1‑151 (MQ=255)
                                            aCTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGt                                                                                                       <  1:3157698/151‑1 (MQ=255)
                                              tGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGc                                                                                                     >  2:8634574/1‑151 (MQ=255)
                                                  gCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTg                                                                                                 <  1:9524319/151‑1 (MQ=255)
                                                   cTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGc                                                                                                <  2:7177257/151‑1 (MQ=255)
                                                    tGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCt                                                                                               <  1:4220689/151‑1 (MQ=255)
                                                    tGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCt                                                                                               <  2:7556801/151‑1 (MQ=255)
                                                    tGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCt                                                                                               >  2:8858105/1‑151 (MQ=255)
                                                     gTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGTGGCGGTGTCCCGCCGCGTGGAACTCTACGAGTCTGGCTTGCGTGGGGTGCGCGGTGCCTTGCTg                                                                                              >  2:1231958/1‑151 (MQ=255)
                                                     gTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTg                                                                                              <  1:1066411/151‑1 (MQ=255)
                                                     gTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTg                                                                                              >  1:7241545/1‑151 (MQ=255)
                                                     gTCCGGGCTGGGCGCCTGGGGGCTGGCGATCGACAACGTGCAAAAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGATGGACTAGGCGGTGACCCGCCGAGTGTAACTCTACCAGGATGGCTTGCGTGGGGTGCGCGGTGCCGTGCTg                                                                                              >  2:677955/1‑151 (MQ=255)
                                                      tCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGa                                                                                             >  1:4463332/1‑151 (MQ=255)
                                                        cGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACa                                                                                           >  2:9330201/1‑151 (MQ=255)
                                                        cGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACa                                                                                           <  2:1734138/151‑1 (MQ=255)
                                                         gggCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAg                                                                                          >  1:1705798/1‑151 (MQ=255)
                                                         gggCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAg                                                                                          >  1:8408299/1‑151 (MQ=255)
                                                          ggCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGc                                                                                         <  2:515629/151‑1 (MQ=255)
                                                            cTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCgg                                                                                       >  2:11224380/1‑151 (MQ=255)
                                                                 cgcCTGGGGGCTGGCGCTGGACAACGAGCAACAGGCGCGGAAGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGAGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGGGCGCGGTGCCTTGCGGACAGCGGGCGAg                                                                                  >  2:4259332/1‑151 (MQ=255)
                                                                    cTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGgcg                                                                               <  2:4481997/151‑1 (MQ=255)
                                                                    cTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGgcg                                                                               <  1:1462525/151‑1 (MQ=255)
                                                                    cTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGgcg                                                                               <  2:5429459/151‑1 (MQ=255)
                                                                    cTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGgcg                                                                               >  2:5910724/1‑151 (MQ=255)
                                                                        gggCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGcaca                                                                           <  1:5479714/151‑1 (MQ=255)
                                                                          gCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATc                                                                         <  1:8890703/151‑1 (MQ=255)
                                                                          gCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATc                                                                         <  1:5945424/151‑1 (MQ=255)
                                                                          gCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATc                                                                         <  2:9778540/151‑1 (MQ=255)
                                                                           cTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCg                                                                        <  2:5778797/151‑1 (MQ=255)
                                                                            tGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGa                                                                       <  1:10532200/151‑1 (MQ=255)
                                                                              gcgcTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGAcc                                                                     <  1:6646746/151‑1 (MQ=255)
                                                                                 cTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACcgcg                                                                  >  2:3082260/1‑151 (MQ=255)
                                                                                  tGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGa                                                                 >  1:8467995/1‑151 (MQ=255)
                                                                                    gTCAACGAGCATCAGGCGCGGATGGCGCTGGACCGCGAGGCGGAACTGCTGGCCGTGGCGGTGACCCGCCGCGTGGAACTCTACCAGTAGGGCTTGCGTGGGGTGCGCGGTGCCTGGCTGACAGCGGGCGAGGCGCACATCGACCGCGTAc                                                               <  2:3879598/151‑1 (MQ=255)
                                                                                        aCGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGtt                                                           <  1:9135956/151‑1 (MQ=255)
                                                                                          gAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTTTGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTcc                                                         <  1:4639565/151‑1 (MQ=255)
                                                                                          gAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTcc                                                         >  2:1502599/1‑151 (MQ=255)
                                                                                          gAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTcc                                                         <  2:11109383/151‑1 (MQ=255)
                                                                                             cAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTccgcc                                                      >  1:2394965/1‑151 (MQ=255)
                                                                                                cAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCt                                                   <  1:11106366/151‑1 (MQ=255)
                                                                                                 aGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTa                                                  >  1:7175776/1‑151 (MQ=255)
                                                                                                 aGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTa                                                  <  2:7343439/151‑1 (MQ=255)
                                                                                                  ggCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTAc                                                 <  1:7418375/151‑1 (MQ=255)
                                                                                                  ggCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTAc                                                 <  2:6300590/151‑1 (MQ=255)
                                                                                                  ggCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTAc                                                 >  1:3959667/1‑151 (MQ=255)
                                                                                                    cgcgGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAg                                               <  2:10232010/151‑1 (MQ=255)
                                                                                                    cgcgGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAg                                               <  1:4865983/151‑1 (MQ=255)
                                                                                                       ggATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCt                                            <  2:7706700/151‑1 (MQ=255)
                                                                                                       ggATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCt                                            >  2:3263861/1‑151 (MQ=255)
                                                                                                       ggATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGGGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCt                                            >  2:2516288/1‑151 (MQ=255)
                                                                                                        gATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTg                                           >  2:9405601/1‑151 (MQ=255)
                                                                                                              gcTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGACCcgc                                     >  2:6565119/1‑151 (MQ=255)
                                                                                                              gcTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGACCcgc                                     >  1:6462743/1‑151 (MQ=255)
                                                                                                              gcTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTAGGGCTTGCGTGGGGTGCGCGGGGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGACCcgc                                     <  1:2875265/151‑1 (MQ=255)
                                                                                                                     cgcgAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGACCCGCGACATCg                              <  1:118334/151‑1 (MQ=255)
                                                                                                                        gAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCGACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGACCCGCGACATCGAcc                           <  1:2611635/151‑1 (MQ=255)
                                                                                                                          ggCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGACCCGCGACATCGACcgc                         >  1:7025811/1‑151 (MQ=255)
                                                                                                                           gCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGACCCGCGACATCGACcgcg                        <  2:4947415/151‑1 (MQ=255)
                                                                                                                           gCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGACCCGCGACATCGACcgcg                        >  2:10028561/1‑151 (MQ=255)
                                                                                                                             ggAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGACCCGCGACATCGACCGCGAg                      >  1:8125781/1‑151 (MQ=255)
                                                                                                                             ggAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGACCCGCGACATCGACCGCGAg                      >  1:3022983/1‑151 (MQ=255)
                                                                                                                              gAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGACCCGCGACATCGACCGCGAGt                     <  2:10117301/151‑1 (MQ=255)
                                                                                                                                  tgctgGCCGAGGCGGTGACCCGCCGCGTGGACCTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGCCAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGACCCGCGACATCGACCGCGAGTTTcc                 <  2:5589795/151‑1 (MQ=255)
                                                                                                                                   gctgGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGACCCGCGACATCGACCGCGAGTTTccc                >  1:3527808/1‑151 (MQ=255)
                                                                                                                                    ctgGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGACCCGCGACATCGACCGCGAGTTTCCCg               >  2:4172771/1‑151 (MQ=255)
                                                                                                                                     tgGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGACCCGCGACATCGACCGCGAGTTTCCCgg              >  1:6173582/1‑151 (MQ=255)
                                                                                                                                     tgGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGACCCGCGACATCGACCGCGAGTTTCCCgg              <  1:7790579/151‑1 (MQ=255)
                                                                                                                                      ggCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGACCCGCGACATCGACCGCGAGTTTCCCGgc             >  2:6979224/1‑151 (MQ=255)
                                                                                                                                       gCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGACCCGCGACATCGACCGCGAGTTTCCCGgcg            >  2:9893625/1‑151 (MQ=255)
                                                                                                                                        ccGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGACCCGCGACATCGACCGCGAGTTTCCCGgcgc           >  1:10086514/1‑151 (MQ=255)
                                                                                                                                            ggCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGACCCGCGACATCGACCGCGAGTTTCCCGGCGCCcgc       <  2:460311/151‑1 (MQ=255)
                                                                                                                                            ggCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGACCCGCGACATCGACCGCGAGTTTCCCGGCGCCcgc       <  2:2939598/151‑1 (MQ=255)
                                                                                                                                              cGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGACCCGCGACATCGACCGCGAGTTTCCCGGCGCCCGcgg     <  2:11003124/151‑1 (MQ=255)
                                                                                                                                               ggTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGACCCGCGACATCGACCGCGAGTTTCCCGGCGCCCGcggc    >  1:8783601/1‑151 (MQ=255)
                                                                                                                                               ggTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGACCCGCGACATCGACCGCGAGTTTCCCGGCGCCCGcggc    >  1:50493/1‑151 (MQ=255)
                                                                                                                                                gTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGACCCGCGACATCGACCGCGAGTTTCCCGGCGCCCGcggct   <  2:6204070/151‑1 (MQ=255)
                                                                                                                                                gTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGACCCGCGACATCGACCGCGAGTTTCCCGGCGCCCGcggct   >  1:4829459/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
AAGTTCAGGCTGAGCCGCTGGGCGGGCGCGACACTGGCGCTCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGATCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGACCCGCGACATCGACCGCGAGTTTCCCGGCGCCCGCGGCTT  >  NC_008463/927769‑928064

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑