Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 1,880,872 C→G A502G (GCC→GGC)  PA14_RS08740 → DEAD/DEAH box helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084631,880,8720CG99.8% 2314.9 / NA 593A502G (GCC→GGC) PA14_RS08740DEAD/DEAH box helicase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base G (284/308);  minor base T (1/0);  total (285/308)
Fisher's exact test for biased strand distribution p-value = 4.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 595 total aligned reads displayed.

CCGCGCCGAGCCCTACGCCGGGCTGGAGCGCGAGCGCTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGCCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCGGTGGAGAGCCTGGCCGGCGACGTGTTCCAACTGGG  >  NC_008463/1880726‑1881019
                                                                                                                                                  |                                                                                                                                                   
 cgcgCCGAGCCCTACGCCGGGCTGGAGCGCGAGCGCTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGgcgcgc                                                                                                                                                >  2:5703199/1‑151 (MQ=255)
   cgccgcGCCCTACGCCGGGCTGGAGCGCGAGCGCTTCGACGAGGTCCTGCGGATGCGCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCgg                                                                                                                                              <  1:7031167/151‑1 (MQ=255)
    gcCGAGCCCTACGCCGGGCTGGAGCGCGAGCGCTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGc                                                                                                                                             <  2:3005688/151‑1 (MQ=255)
     ccGAGCCCTACGCCGGGCTGGAGCGCGAGCGCTTCGACGAAGTCCTGCGGATGCTCGCCGTGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCt                                                                                                                                            <  1:147848/151‑1 (MQ=255)
      cGAGCCCTACGCCGGGCTGGAGCGCGAGCGCTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTg                                                                                                                                           <  1:3366863/151‑1 (MQ=255)
      cGAGCCCTACGCCGGGCTGGAGCGCGAGCGCTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTg                                                                                                                                           >  2:10109079/1‑151 (MQ=255)
      cGAGCCCTACGCCGGGCTGGAGCGCGAGCGCTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTg                                                                                                                                           <  1:2591600/151‑1 (MQ=255)
      cGAGCCCTACGCCGGGCTGGAGCGCGAGCGCTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGGGGCCGCCGTGGCGCGCGGCTg                                                                                                                                           >  2:4743192/1‑151 (MQ=255)
       gAGCCCTACGCCGGGCTGGAGCGCGAGCGCTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGa                                                                                                                                          >  2:3826534/1‑151 (MQ=255)
        aGCCCTACGCCGGGCTGGAGCGCGAGCGCTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGAc                                                                                                                                         <  1:2150739/151‑1 (MQ=255)
        aGCCCTACGCCGGGCTGGAGCGCGAGCGCTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACAACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGAc                                                                                                                                         >  2:2587722/1‑151 (MQ=255)
         gCCCTACGCCGGGCTGGAGCGCGAGCGCTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGAcc                                                                                                                                        <  2:6435619/151‑1 (MQ=255)
            cTACGCCGGGCTGGAGCGCGAGCGCTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACcgcg                                                                                                                                     <  1:10405013/151‑1 (MQ=255)
                gCCGGGCTGGAGCGCGAGCGCTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGa                                                                                                                                 <  1:10613758/151‑1 (MQ=255)
                gCCGGGCTGGAGCGCGAGCGCTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGa                                                                                                                                 <  2:9520854/151‑1 (MQ=255)
                  cGGGCTGGAGCGCGAGCGCTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGAcc                                                                                                                               >  1:6506831/1‑151 (MQ=255)
                     gCTGGAGCGCGAGCGCTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTcg                                                                                                                            <  1:1941056/151‑1 (MQ=255)
                       tGGAGCGCGAGCGCTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTcggg                                                                                                                          <  1:2495449/151‑1 (MQ=255)
                        ggAGCGCGAGCGCTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTcgggc                                                                                                                         <  2:7601436/151‑1 (MQ=255)
                        ggAGCGCGAGCGCTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTcgggc                                                                                                                         >  1:9200006/1‑151 (MQ=255)
                        ggAGCGCGAGCGCTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTcgggc                                                                                                                         >  2:8902087/1‑151 (MQ=255)
                         gagcgcgagcgcTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTcgggcg                                                                                                                        >  1:7261227/1‑151 (MQ=255)
                               gagcgcTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCa                                                                                                                  >  1:7706330/1‑151 (MQ=255)
                                agcgcTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCAt                                                                                                                 <  2:11238512/151‑1 (MQ=255)
                                 gcgcTTCGCCGACGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCAtt                                                                                                                <  2:7314225/151‑1 (MQ=255)
                                  cgcTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTc                                                                                                               <  2:1885121/151‑1 (MQ=255)
                                   gcTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTcc                                                                                                              >  2:4304944/1‑151 (MQ=255)
                                     ttCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCg                                                                                                            >  1:387229/1‑151 (MQ=255)
                                       cgacgaAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGAt                                                                                                          >  2:3742061/1‑151 (MQ=255)
                                        gacgaAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATa                                                                                                         >  1:1144486/1‑151 (MQ=255)
                                         acgaAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATAc                                                                                                        >  1:4697492/1‑151 (MQ=255)
                                           gaAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCg                                                                                                      <  1:1766241/151‑1 (MQ=255)
                                           gaAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCg                                                                                                      >  1:7899983/1‑151 (MQ=255)
                                            aaGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCgg                                                                                                     >  2:630945/1‑151 (MQ=255)
                                             aGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGc                                                                                                    <  1:5021850/151‑1 (MQ=255)
                                              gTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCg                                                                                                   >  2:9158327/1‑151 (MQ=255)
                                                 cTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCAGTCCCGATACCGGCGACt                                                                                                >  1:7593682/1‑151 (MQ=255)
                                                       aTGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCg                                                                                          >  1:9485055/1‑151 (MQ=255)
                                                          cTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGtgc                                                                                       >  2:5161305/1‑151 (MQ=255)
                                                           tCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGtgct                                                                                      <  1:5409389/151‑1 (MQ=255)
                                                             gCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGtgctgc                                                                                    >  2:4507123/1‑151 (MQ=255)
                                                             gCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGtgctgc                                                                                    >  1:8250432/1‑151 (MQ=255)
                                                               cGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGctgctg                                                                                  >  1:10863519/1‑151 (MQ=255)
                                                               cGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGctgctg                                                                                  <  1:1315007/151‑1 (MQ=255)
                                                               cGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGctgctg                                                                                  >  2:10376728/1‑151 (MQ=255)
                                                                gAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTgg                                                                                 >  2:3432576/1‑151 (MQ=255)
                                                                   ggCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGc                                                                              <  2:3308836/151‑1 (MQ=255)
                                                                    gCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGcc                                                                             <  2:7025039/151‑1 (MQ=255)
                                                                     cTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCg                                                                            <  2:146728/151‑1 (MQ=255)
                                                                        ccACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAg                                                                         <  1:102472/151‑1 (MQ=255)
                                                                        ccACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAg                                                                         >  1:4376622/1‑151 (MQ=255)
                                                                         cACTCACGGCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAgg                                                                        <  2:11064860/151‑1 (MQ=255)
                                                                         cACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACGACAGCGTGCTGCTGGAGCCGCAgg                                                                        >  2:4072261/1‑151 (MQ=255)
                                                                             cACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCct                                                                    <  1:1050778/151‑1 (MQ=255)
                                                                               cGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCctgc                                                                  >  1:5848859/1‑151 (MQ=255)
                                                                               cGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCctgc                                                                  >  2:2800881/1‑151 (MQ=255)
                                                                               cGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCAATCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCctgc                                                                  >  2:1739002/1‑151 (MQ=255)
                                                                                gCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCctgct                                                                 >  2:2105711/1‑151 (MQ=255)
                                                                                 ccTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCctgctg                                                                <  1:11084827/151‑1 (MQ=255)
                                                                                  cTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCtgg                                                               >  1:4106205/1‑151 (MQ=255)
                                                                                     ggCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCtggtgg                                                            <  1:8601009/151‑1 (MQ=255)
                                                                                             cggcgCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTc                                                    <  2:7997045/151‑1 (MQ=255)
                                                                                             cggcgCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTc                                                    <  1:8794428/151‑1 (MQ=255)
                                                                                                cgcCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAAc                                                 <  1:3240561/151‑1 (MQ=255)
                                                                                                cgcCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAAc                                                 <  1:2450928/151‑1 (MQ=255)
                                                                                                 gcCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACg                                                >  1:10213641/1‑151 (MQ=255)
                                                                                                 gcCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACg                                                >  2:2376753/1‑151 (MQ=255)
                                                                                                   cTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGGGGGAACGGTCAACGAg                                              >  1:992230/1‑151 (MQ=255)
                                                                                                    tATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAgg                                             <  1:10098135/151‑1 (MQ=255)
                                                                                                     aTCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGCTACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGa                                            <  2:10825181/151‑1 (MQ=255)
                                                                                                      tctcCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGACCGGTCAACGAGGAc                                           >  1:10942016/1‑151 (MQ=255)
                                                                                                        tcCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACtt                                         <  1:2940042/151‑1 (MQ=255)
                                                                                                         ccATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTc                                        <  1:7209837/151‑1 (MQ=255)
                                                                                                          cATCGCGATGCGCTCTACGGCCTGCTCCGTGGCCGCCGTGGCGCGAGGCTGACCGCTCTGACCTCGGGCGGGACCATTACCGATACCGGCGTCTACTGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTcg                                       <  2:7867768/151‑1 (MQ=255)
                                                                                                          cATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTcg                                       <  2:8561902/151‑1 (MQ=255)
                                                                                                             cgcgATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGACCGGTCAACGAGGACTTCGCgg                                    >  2:8117255/1‑151 (MQ=255)
                                                                                                               cgATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCGGTg                                  >  1:670408/1‑151 (MQ=255)
                                                                                                                gATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCGGTgg                                 >  2:9848970/1‑151 (MQ=255)
                                                                                                                   gcgcTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCGGTGgaga                              <  1:2739176/151‑1 (MQ=255)
                                                                                                                   gcgcTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCGGTGgaga                              <  1:8761238/151‑1 (MQ=255)
                                                                                                                   gcgcTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCGGTGgaga                              >  1:6316474/1‑151 (MQ=255)
                                                                                                                    cgcTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCGGTGGagag                             >  2:5419067/1‑151 (MQ=255)
                                                                                                                         aaCGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCCACGAGGACTTCGCGGTGGAGAGCCTgg                        >  2:10604587/1‑151 (MQ=255)
                                                                                                                            ggCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCGGTGGAGAGCCTGGCCg                     >  2:9349544/1‑151 (MQ=255)
                                                                                                                             gCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCGGTGGAGAGCCTGGCCgg                    <  2:10163317/151‑1 (MQ=255)
                                                                                                                               cTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCGGACCTCGGGCGGGACCAGTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCGGTGGAGAGCCTGGCCGGCg                  <  2:6936737/151‑1 (MQ=255)
                                                                                                                                 gCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCGGTGGAGAGCCTGGCCGGCGAc                <  2:6709189/151‑1 (MQ=255)
                                                                                                                                 gCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCGGTGGAGAGCCTGGCCGGCGAc                <  2:9331857/151‑1 (MQ=255)
                                                                                                                                   tCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCTGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCGGTGGAGAGCCTGGCCGGCGACgt              >  1:9665709/1‑151 (MQ=255)
                                                                                                                                   tCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCGGTGGAGAGCCTGGCCGGCGACgt              >  2:4586736/1‑151 (MQ=255)
                                                                                                                                     cGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCGGTGGAGAGCCTGGCCGGCGACgtgt            <  1:5545164/151‑1 (MQ=255)
                                                                                                                                       tGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCGGTGGAGAGCCTGGCCGGCGACGTGTTc          <  1:5641602/151‑1 (MQ=255)
                                                                                                                                        ggCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCGGTGGAGAGCCTGGCCGGCGACGTGTTcc         <  2:8783906/151‑1 (MQ=255)
                                                                                                                                            gccgTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCGGTGGAGAGCCTGGCCGGCGACGTGTTCCAACt     >  2:8352751/1‑151 (MQ=255)
                                                                                                                                            gccgTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCGGTGGAGAGCCTGGCCGGCGACGTGTTCCAACt     <  1:8043488/151‑1 (MQ=255)
                                                                                                                                            gccgTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCGGTGGAGAGCCTGGCCGGCGACGTGTTCCAACt     >  1:4208092/1‑151 (MQ=255)
                                                                                                                                             ccgTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCCGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCGGTGGAGAGCCTGGCCGGCGACGTGTTCCAAcgg    >  2:351103/1‑149 (MQ=255)
                                                                                                                                             ccgTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCGGTGGAGAGCCTGGCCGGCGACGTGTTCCAACTg    <  1:8929423/151‑1 (MQ=255)
                                                                                                                                             ccgTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCGGTGGAGAGCCTGGCCGGCGACGTGTTCCAACTg    >  2:6110760/1‑151 (MQ=255)
                                                                                                                                               gTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCGGTGGAGAGCCTGGCCGGCGACGTGTTCCAACTggg  >  1:6946826/1‑151 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
CCGCGCCGAGCCCTACGCCGGGCTGGAGCGCGAGCGCTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGCCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCGGTGGAGAGCCTGGCCGGCGACGTGTTCCAACTGGG  >  NC_008463/1880726‑1881019

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑