Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 2,354,149 A→G intergenic (‑34/+17) PA14_RS10945 ← / ← lipA lipase secretion chaperone/triacylglycerol lipase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084632,354,1490AG99.7% 1411.4 / ‑5.6 373intergenic (‑34/+17)PA14_RS10945/lipAlipase secretion chaperone/triacylglycerol lipase
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (171/201);  total (171/202)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 373 total aligned reads displayed.

CCGCCTGCGCGCTGGCCGGGGGCGCCGTCTCGGGTGCGGGGGAAGGTTCCAGCCAGACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAAGCCCCGGCCGGGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCACCTCGTCCAGGTGGTTCATCCGGTAGTTGTCGCGGAT  >  NC_008463/2354001‑2354297
                                                                                                                                                    |                                                                                                                                                    
ccgccTGCGCGCTGGCCGGGGGCGCCGTCTCGGGTGCGGGGGAAGGTTCCAGCCAGACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGc                                                                                                                                                    <  1:1985126/151‑1 (MQ=255)
   ccTGCGCGCTGGCCGGGGGCGCCGTCTCGGGTGCGGGGGAAGGTTCCAGCCAGACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGcccc                                                                                                                                                 <  2:2088271/151‑1 (MQ=255)
   ccTGCGCGCTGGCCGGGGGCGCCGTCTCGGGTGCGGGGGAAGGTTCCAGCCAGACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGcccc                                                                                                                                                 <  2:10554004/151‑1 (MQ=255)
   ccTGCGCGCTGGCCGGGGGCGCCGTCTCGGGTGCGGGGGAAGGTTCCAGCCAGACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGcccc                                                                                                                                                 >  1:2612926/1‑151 (MQ=255)
    cTGCGCGCTGGCCGGGGGCGCCGTCTCGGGTGCGGGGGAAGGTTCCAGCCAGACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCccg                                                                                                                                                <  1:4776532/151‑1 (MQ=255)
     tGCGCGCTGGCCGGGGGCGCCGTCTCGGGTGCGGGGGAAGGTTCCAGCCAGACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCccgg                                                                                                                                               >  1:6543934/1‑151 (MQ=255)
       cgcgcTGGCCGGGGGCGCCGTCTCGGGTGCGGGGGAAGGTTCCAGCCAGACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCccggcc                                                                                                                                             >  1:7385463/1‑151 (MQ=255)
         cgcTGGCCGGGGGCGCCGTCTCGGGTGCGGGGGAAGGTTCCAGCCAGACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCccggccgg                                                                                                                                           <  1:5005136/151‑1 (MQ=255)
            tGGCCGGGGGCGCCGTCTCGGGTGCGGGGGAAGGTTCCAGCCAGACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGCGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCCggggt                                                                                                                                        <  2:4850107/151‑2 (MQ=255)
            tGGCCGGGGGCGCCGTCTCGGGTGCGGGGGAAGGTTCCAGCCAGACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCCggggt                                                                                                                                        >  2:5768448/1‑150 (MQ=255)
             ggCCGGGGGCGCCGTCTCGGGTGCGGGGGAAGGTTCCAGCCAGACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCCggggtc                                                                                                                                       <  2:7364697/151‑3 (MQ=255)
                 gggggCGCCGTCTCGGGTGCGGGGGAAGGTTCCAGCCAGACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTAc                                                                                                                                  >  2:3157604/1‑151 (MQ=255)
                 gggggCGCCGTCTCGGGTGCGGGGGAAGGTTCCAGCCAGACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTAc                                                                                                                                  <  2:10490902/151‑1 (MQ=255)
                  ggggCGCCGTCTCGGGTGCGGGGGAAGGTTCCAGCCCGCCGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACa                                                                                                                                 <  1:353748/151‑1 (MQ=255)
                  ggggCGCCGTCTCGGGTGCGGGGGAAGGTTCCAGCCAGACGACCCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACa                                                                                                                                 <  2:8251825/151‑1 (MQ=255)
                   gggCGCCGTCTCGGGTGCGGGGGAAGGTTCCAGCCAGACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCTGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAg                                                                                                                                <  1:533911/151‑1 (MQ=255)
                    ggCGCCGTCTCGGGTGCGGGGGAAGGTTCCAGCCAGACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAgg                                                                                                                               <  2:9343169/151‑1 (MQ=255)
                     gcgcCGTCTCGGGTGCGGGGGAAGGTTCCAGCCAGACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGc                                                                                                                              >  2:1037230/1‑151 (MQ=255)
                      cgcCGTCTCGGGTGCGGGGGACGGTTCCAGCCAGACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCt                                                                                                                             <  2:8668330/151‑1 (MQ=255)
                       gcCGTCTCGGGTGCGGGGGAAGGTTCCAGCCAGACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGTCTg                                                                                                                            >  1:8443944/1‑151 (MQ=255)
                         cGTCTCGGGTGCGGGGGAAGGTTCCAGCCAGACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGc                                                                                                                          >  1:1406461/1‑151 (MQ=255)
                          gTCTCGGGTGCGGGGGAAGGTTCCAGCCAGACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCg                                                                                                                         >  2:7737837/1‑151 (MQ=255)
                                 gTGCGGGGGAAGGTTCCAGCCAGACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCa                                                                                                                  <  1:2337480/151‑1 (MQ=255)
                                  tGCGGGGTAAGGTTCCAGCCAGACGAACCAGGCCTGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAg                                                                                                                 <  1:3911511/151‑1 (MQ=255)
                                  tGCGGGGGAAGGTTCCAGCCAGACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAg                                                                                                                 <  2:8463723/151‑1 (MQ=255)
                                   gCGGGGGAAGGTTCCAGCCAGACGAACCAGGCCAGGCTGGCGGCGACCGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGTGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAgg                                                                                                                <  1:4434230/151‑1 (MQ=255)
                                   gCGGGGGAAGGTTCCAGCCAGACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAgg                                                                                                                <  2:11230430/151‑1 (MQ=255)
                                    cGGGGGAAGGTTCCAGCCAGACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGc                                                                                                               >  2:2030560/1‑151 (MQ=255)
                                    cGGGGGAAGGTTCCAGCCAGACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGc                                                                                                               >  1:623328/1‑151 (MQ=255)
                                      ggggAAGGTTCCAGCCAGACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGAAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGGTCTTCAGGCgg                                                                                                             >  2:2701839/1‑151 (MQ=255)
                                        ggAAGGTTCCAGCCAGACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGtt                                                                                                           >  1:9386720/1‑151 (MQ=255)
                                            ggTTCCAGCCAGACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCg                                                                                                       <  2:657881/151‑1 (MQ=255)
                                             gTTCCAGCCAGACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAATGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGt                                                                                                      >  1:9508746/1‑151 (MQ=255)
                                             gTTCCAGCCAGACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGt                                                                                                      <  2:6912550/151‑1 (MQ=255)
                                              gtCCAGCCAGCCGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTg                                                                                                     <  2:10145200/150‑1 (MQ=255)
                                               tCCACCCAGCCGAACCAGGCCAGGCGGGCGGCGACCGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGc                                                                                                    <  1:1095138/151‑1 (MQ=255)
                                                  agccagACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTgg                                                                                                 <  1:3605128/151‑1 (MQ=255)
                                                   gccagACGAACCTGGCCTGGCTGGCGGCGAACGCCAGTGGATTCTGCTGGAGGATTTTCTTCACGCGTGGGGTCTTCCGGTAAAGGGCGGGGGCCGAGGCCCGGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGc                                                                                                <  1:8752762/151‑1 (MQ=255)
                                                    ccagACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCg                                                                                               <  2:7115399/151‑1 (MQ=255)
                                                      agACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGt                                                                                             >  1:4305577/1‑151 (MQ=255)
                                                       gACGAACCAGGCCAGGCTGGCGGCGAACGCCCGTGGAATCAGCAGGAGGATTATCTTCACGCGAGGGGTCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGATGGCGTGCTGGCGGtc                                                                                            >  2:613674/1‑150 (MQ=255)
                                                       gACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTa                                                                                            <  2:2784790/151‑1 (MQ=255)
                                                       gACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTa                                                                                            >  1:3966685/1‑151 (MQ=255)
                                                        aCGAACCAGGCCCGGCTGGCGGCGAACGCCAGTGTACTAAGCAGGAGGATGTTCTTCCCGCGAGGGGGCGTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAg                                                                                           <  1:5702297/151‑1 (MQ=255)
                                                        aCGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAg                                                                                           >  2:10890623/1‑151 (MQ=255)
                                                        aCGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAg                                                                                           <  1:6087511/151‑1 (MQ=255)
                                                         cGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGa                                                                                          >  1:5230881/1‑151 (MQ=255)
                                                           aaCCCGGCCAGGCGGGCGGCGAACGCCAGTGGAATCAGCCGGAGGATTTACTTCACGCGAGGGGGCTACCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACg                                                                                        <  1:7660213/151‑1 (MQ=255)
                                                            aCCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGTGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACgg                                                                                       >  1:637251/1‑150 (MQ=255)
                                                            aCCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTACACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGc                                                                                       <  2:3448283/151‑1 (MQ=255)
                                                                ggccaggcTGGCGGCGAACGCCCGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGAc                                                                                   >  1:7123952/1‑151 (MQ=255)
                                                                 gccaggcTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCTGGCGGTTGGCGTGCTGGCGGTAGACGCTGAcc                                                                                  >  1:7191508/1‑151 (MQ=255)
                                                                 gccaggcTGGCGGCCAACGCCAGTGGAATCAGCAGGAGGATTTTCATCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGAcc                                                                                  <  1:5276255/151‑1 (MQ=255)
                                                                  ccaggcTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGAAGACGCTGACCg                                                                                 >  2:4652960/1‑151 (MQ=255)
                                                                    aggcTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCggg                                                                               >  1:5061354/1‑151 (MQ=255)
                                                                      gcTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCt                                                                             >  1:4418697/1‑151 (MQ=255)
                                                                        tGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTgg                                                                           >  1:6727726/1‑151 (MQ=255)
                                                                           cggcgAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGtct                                                                        >  1:2778843/1‑151 (MQ=255)
                                                                           cggcgAACGCCAGTGGAATCAGCAGGAGGATTTTCTACACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGtct                                                                        <  1:893364/151‑1 (MQ=255)
                                                                                aaCGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAAc                                                                   <  2:10845125/151‑1 (MQ=255)
                                                                                 aCGCCAGTGGACTCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCTGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACa                                                                  <  2:3958873/151‑1 (MQ=255)
                                                                                 aCGCCAGTGGAATCAGCAGGAGGATTTTCGTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACa                                                                  <  2:2347742/151‑1 (MQ=255)
                                                                                   gCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAgg                                                                >  1:3040330/1‑151 (MQ=255)
                                                                                            aTCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAgg                                                       <  2:9603502/151‑1 (MQ=255)
                                                                                              cagcagGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGcc                                                     <  2:3348495/151‑1 (MQ=255)
                                                                                                gcagGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGa                                                   <  2:10234084/151‑1 (MQ=255)
                                                                                                  aggaggATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAg                                                 >  2:3527116/1‑151 (MQ=255)
                                                                                                     aggaTTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGAcc                                              <  2:415929/151‑1 (MQ=255)
                                                                                                       gaTTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTg                                            <  2:4536005/151‑1 (MQ=255)
                                                                                                         ttttCTTAACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGCGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGt                                          <  2:5336207/151‑1 (MQ=255)
                                                                                                          tttCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGtt                                         <  1:9085633/151‑1 (MQ=255)
                                                                                                           ttcttcACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTc                                        >  2:6298750/1‑151 (MQ=255)
                                                                                                           gtcttcACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTc                                        <  1:1104729/150‑1 (MQ=255)
                                                                                                            tcttcACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCa                                       <  1:380849/151‑1 (MQ=255)
                                                                                                            tcttcACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCa                                       <  2:4081522/151‑1 (MQ=255)
                                                                                                             cttcACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCAc                                      >  2:5646363/1‑151 (MQ=255)
                                                                                                              ttcACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCAcc                                     >  1:10026952/1‑151 (MQ=255)
                                                                                                              ttcACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCAcc                                     <  1:700187/151‑1 (MQ=255)
                                                                                                               tcACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCACCt                                    >  1:7790252/1‑151 (MQ=255)
                                                                                                                cACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCACCTc                                   >  1:9849916/1‑151 (MQ=255)
                                                                                                                 aCGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCACCTCg                                  >  2:9007773/1‑151 (MQ=255)
                                                                                                                    cgAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCACCTCGTcc                               <  2:1223241/151‑1 (MQ=255)
                                                                                                                     gAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCACCTCGTCCa                              >  2:5620907/1‑151 (MQ=255)
                                                                                                                       gggggCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCACCTCGTCCAgg                            >  2:11089316/1‑151 (MQ=255)
                                                                                                                         gggCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCACCTCGTCCAggtg                          <  2:2503985/151‑1 (MQ=255)
                                                                                                                           gCTGCCGGGACAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCACCTCGTCCAggtggt                        <  2:10734975/151‑1 (MQ=255)
                                                                                                                               ccGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCACCTCGTCCAGGTGGTTCAt                    >  1:8304367/1‑151 (MQ=255)
                                                                                                                                 gggAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCACCTCGTCCAGGTGGTTCATcc                  >  1:1634228/1‑151 (MQ=255)
                                                                                                                                  ggAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCACCTCGTCCAGGTGGTTCATCCg                 >  2:1870628/1‑151 (MQ=255)
                                                                                                                                  ggAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCACCTCGTCCAGGTGGTTCATCCg                 <  2:1678793/151‑1 (MQ=255)
                                                                                                                                  ggAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCACCTCGTCCAGGTGGTTCATCCg                 <  1:9759450/151‑1 (MQ=255)
                                                                                                                                  ggAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCACCTCGTCCAGGTGGTTCATCCg                 <  1:1509703/151‑1 (MQ=255)
                                                                                                                                     aaGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCACCTCGTCCAGGTGGTTCATCCGGTa              >  1:8605634/1‑151 (MQ=255)
                                                                                                                                     aaGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCACCTCGTCCAGGTGGTTCATCCGGTa              >  1:7941749/1‑151 (MQ=255)
                                                                                                                                       gggccggggccgAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCACCTCGTCCAGGTGGTTCATCCGGTAGt            <  2:780725/151‑1 (MQ=255)
                                                                                                                                        ggccggggccgCGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCACCTCGTCCAGGTGGTTCATCCGGTAGtt           <  2:3778500/151‑1 (MQ=255)
                                                                                                                                           cggggccgAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCACCTCGTCCAGGTGGTTCATCCGGTAGTTGTc        <  1:10555686/151‑1 (MQ=255)
                                                                                                                                             gggccgAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCACCTCGTCCAGGTGGTTCATCCGGTAGTTGTcgc      >  2:7533929/1‑151 (MQ=255)
                                                                                                                                              ggccgCGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCACCTCGTCCAGGTGGTTCATCCGGTAGTTGTcgcg     <  1:2111816/151‑1 (MQ=255)
                                                                                                                                                 cgAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCACCTCGTCCAGGTGGTTCATCCGGTAGTTGTCGCGGAt  <  2:5972616/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CCGCCTGCGCGCTGGCCGGGGGCGCCGTCTCGGGTGCGGGGGAAGGTTCCAGCCAGACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAAGCCCCGGCCGGGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCACCTCGTCCAGGTGGTTCATCCGGTAGTTGTCGCGGAT  >  NC_008463/2354001‑2354297

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑