Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 560,192 A→C 5.7% noncoding (14/77 nt) argU → tRNA‑Arg

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273560,1920AC 5.7% 755.3 / 2.2 225noncoding (14/77 nt)argUtRNA‑Arg
Reads supporting (aligned to +/- strand):  ref base A (101/104);  new base C (2/18);  total (103/122)
Fisher's exact test for biased strand distribution p-value = 6.61e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 230 total aligned reads displayed.

TTGTTTGCCGCTATAGCGAAATAAATCAGAAAATCAGACGCGGTCGTTCACTTGTTCAGCAACCAGATCAAAAGCCATTGACTCAGCAAGGGTTGACCGTATAATTCACGCGATTACACCGCATTGCGGTATCAACGCGCCCTTAGCTCAGTTGGATAGAGCAACGACCTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTACAATTCAATCAGTTACGCCTTCTTTATATCCTCCATAATTTCAGAGTGGGACATATTTGGGACATTATCACCAAAAATGTCGT  >  CP009273/560043‑560341
                                                                                                                                                     |                                                                                                                                                     
ttgtttgCCGCTATAGCGAAATAAATCAGAAAATCAGACGCGGTCGTTCACTTGTTCAGCAACCAGATCAAAAGCCATTGACTCAGCAAGGGTTGACCGTATAATTCACGCGATTACACCGCATTGCGGTATCAACGCGCCCTTAGCTCa                                                                                                                                                       >  2:3021024/1‑150 (MQ=255)
  gtttgCCGCTATAGCGAAATAAATCAGAAAATCAGACGCGGTCGTTCACTTGTTCAGCAACCAGATCAAAAGCCATTGACTCAGCAAGGGTTGACCGTATAATTCACGCGATTACACCGCATTGCGGTATCAACGCGCCCTTAGCTCAGt                                                                                                                                                     <  2:2067628/150‑1 (MQ=255)
  gtttgCCGCTATAGCGAAATAAATCAGAAAATCAGACGCGGTCGTTCACTTGTTCAGCAACCAGATCAAAAGCCATTGACTCAGCAAGGGTTGACCGTATAATTCACGCGATTACACCGCATTGCGGTATCAACGCGCCCTTAGCTCAGt                                                                                                                                                     <  2:4480195/150‑1 (MQ=255)
           tataGCGAAATAAATCAGAAAATCAGACGCGGTCGTTCACTTGTTCAGCAACCAGATCAAAAGCCATTGACTCAGCAAGGGTTGACCGTATAATTCACGCGATTACACCGCATTGCGGTATCAACGCGCCCTTAGCTCAGTTGGATagag                                                                                                                                            >  2:766049/1‑150 (MQ=255)
           tataGCGAAATAAATCAGAAAATCAGACGCGGTCGTTCACTTGTTCAGCAACCAGATCAAAAGCCATTGACTCAGCAAGGGTTGACCGTATAATTCACGCGATTACACCGCATTGCGGTATCAACGCGCCCTTAGCTCAGTTGGATagag                                                                                                                                            <  2:596576/150‑1 (MQ=255)
           tataGCGAAATAAATCAGAAAATCAGACGCGGTCGTTCACATGTTCAGCAACCAGATCAAAAGCCAATGAATCAGCAAGGGTTGACCGTATAATTAACGCGATTACACCCCAATGCGGTATCAAAGCGCCCATAGCTCAGTTGGATagag                                                                                                                                            >  1:189790/1‑150 (MQ=255)
            ataGCGAAATAAATCAGAAAATCAGACGCGGTCGTTCACTTGTTCAGCAACCAGATCAAAAGCCATTGACTCAGCAAGGGTTGACCGTATAATTCACGCGATTACACCGCATTGCGGTATCAACGCGCCCTTAGCTCAGTTGGATAGAGc                                                                                                                                           >  2:1225760/1‑150 (MQ=255)
             taGCGAAATAAATCAGAAAATCAGACGCGGTCGTTCACTTGTTCAGCAACCAGATCAAAAGCCATTGACTCAGCAAGGGTTGACCGTATAATTCACGCGATTACACCGCATTGCGGTATCAACGCGCCCTTAGCTCAGTTGGATAGAGCa                                                                                                                                          <  2:1377300/150‑1 (MQ=255)
             taGCGAAATAAATCAGAAAATCAGACGCGGTCGTTCACTTGTTCAGCAACCAGATAAAAAGCCATTGACTCAGCAAGGGTTGACCGTATAATTCACGCGATTACACCGCATTGCGGTATCAACGCGCCCTTAGCTCAGTTGGATAGAGCa                                                                                                                                          >  2:1916159/1‑150 (MQ=255)
              aGCGAAATAAATCAGAAAATCAGACGCGGTCGTTCACTTGTTCAGCAACCAGATCAAAAGCCATTGACTCAGCAAGGGTTGACCGTATAATTCACGCGATTACACCGCATTGCGGTATCAACGCGCCCTTAGCTCAGTTGGATAGAGCaa                                                                                                                                         <  2:1106471/150‑1 (MQ=255)
               gCGAAATAAATCAGAAAATCAGACGCGGTCGTTCACTTGTTCAGCAACCAGATCAAAAGCCATTGACTCAGCAAGGGTTGACCGTATAATTCACGCGATTACACCGCATTGCGGTATCAACGCGCCCTTAGCTCAGTTGGATAGAGCAAc                                                                                                                                        <  1:2214609/150‑1 (MQ=255)
               gCGAAATAAATCAGAAAATCAGACGCGGTCGTTCACTTGTTCAGCAACCAGATCAAAAGCCATTGACTCAGCAAGGGTTGACCGTATAATTCACGCGATTACACCGCATTGCGGTATCAACGCGCCCTTAGCTCAGTTGGATAGAGCAAc                                                                                                                                        <  2:1903353/150‑1 (MQ=255)
                  aaataaatCAGAAAATCAGACGCGGTCGTTCACTTGTTAAGCAACCAGATCAAAAGCCAATGACTCAGCAAGGGTTGTCCGTATAAATAACGCGATTACACCGCATTGCGGTATCAACGCGCCCTTAGCTCAGTTTGCTAGAGCAACGAc                                                                                                                                     >  1:230300/1‑150 (MQ=255)
                     taaatCAGAAAATCAGACGCGGTCGTTCACTTTTTCAGCAACCAGATAAAAAGCCAATTAATCAGCAAGGGTTTACCGTATAATTCACGCCATTACACCGCATTGCGGTATCAACGCGCCCATAGCTCATTTGGATAGAGCAACGACCtt                                                                                                                                  >  2:189342/1‑150 (MQ=255)
                      aaatcagaaaatcagaCGCGGTCGTTCACTTGTTCAGCAACCAGATCAAAAGCCATTGACTCAGCAAGGGTTGACCGTATAATTCACGCGATTACACCGCATTGCGGTATCAACGCGCCCTTAGCTCAGTTGGATAGAGCAACGACCTTc                                                                                                                                 <  1:2537944/150‑1 (MQ=255)
                      aaatcagaaaatcagaCGCGGTCGTTCACTTGTTCAGCAACCAGATCAAAAGCCATTGACTCAGCAAGGGTTGACCGTATAATTCACGCGATTACACCGCATTGCGGTATCAACGCGCCCTTAGCTCAGTTGGATAGAGCAACGACCTTc                                                                                                                                 <  1:766049/150‑1 (MQ=255)
                       aatcagaaaatcagaCGCGGTCGTTCACTTTTTCAGCAACCAGATAAAAATCCAATGACACAGCAAGGGTTGACCGTATAAATAACGCGATTACACCGCATTGCGGGATCAACGCGCCCATAGCTCAGTTGGATAGAGCAACGACCATCt                                                                                                                                >  1:2936887/1‑150 (MQ=255)
                       aatcagaaaatcagaCGCGGTCGTTCACTTGTTCAGCAACCAGATCAAAAGCCATTGACTCAGCAAGGGTTGACCGTATAATTCACGCGATTACACCGCATTGCGGTATCAACGCGCCCTTAGCTCCGTTGGATAGAGCAACGACCTTCt                                                                                                                                <  1:1425886/150‑1 (MQ=255)
                       aatcagaaaatcagaCGCGGTCGTTCACTTGTTCAGCAACCAGATCAAAAGCCATTGACTAAGCAAGGGTTGACCGTATAATTAACGCGATTACACCGCATTGCGGTATCAACGCGCCCTTATCTCAGTTGGATAGAGCAACGACCTTca                                                                                                                                >  1:4702243/1‑149 (MQ=255)
                       aatcagaaaatcagaCGCGGTCGTTCACTTGTTCAGCAACCAGATCAAAAGCCAGTGACTCAGCAAGGGTTGACCGTATAATTCACGCGATTACACCGCATTGCGGTATCAACGCGCCCTTAGCTCAGTTGGATAGAGCAACGACCTTCt                                                                                                                                >  1:2075288/1‑150 (MQ=255)
                         tcagaaaatcagaCGCGGTCGTTCACTTGTTCAGCAACCAGATCAAAAGCCATTGACTCAGCAAGGGTTGACCGTATAATTCACGCGATTACACCGCATTGCGGTATCAACGCGCCCTTAGCTCAGTTGGATAGAGCAACGACCTTCTaa                                                                                                                              <  2:1940464/150‑1 (MQ=255)
                           agaaaatcagaCGCGGTCGTTAACTTGTTCAGCAACCAGATAAAAAGCCATTGACTCAGCAAGGGTTTACCGTATAATTAACGCGATTACACCGCATTTCGGTATCAACGCGCCCTTAGCTCAGTTGGATAGAGCAACGACCTTCTAAGt                                                                                                                            >  2:4200621/1‑150 (MQ=255)
                                      cgcgGTCGTTCACTTGTTCAGCAACCAGATCAAAAGCCATTGACTCAGCAAGGGTTGACCGTATAATTCACGCGATTACACCGCATTGCGGTATCAACGCGCCCTTAGCTCAGTTGGATAGAGCAACGACCTTCTAAGTCGTGGGCCGCa                                                                                                                 <  1:2575101/150‑1 (MQ=255)
                                      cgcgGTCGTTCACTTGTTCAGCAACCAGATCAAAAGCCATTGACTCAGCAAGGGTTGACCGTATAATTCACGCGATTACACCGCATTGCGGTATCAACGCGCCCTTAGCTCAGTTGGATAGAGCAACGACCTTCTAAGTCGTGGGCCGCa                                                                                                                 <  1:410045/150‑1 (MQ=255)
                                         ggTCGTTCACTTGTTCAGCAACCAGATCAAAAGCCATTGACTCAGCAAGGGTTGACCGTATACTTCACGCGATTACACCGCATTGCGGTATCAACGCGCCCTTAGCTCAGTTGGATAGAGCAACGACCTTCTAAGTCGTGGGCCGCAGGt                                                                                                              >  2:3723979/1‑150 (MQ=255)
                                         ggTCGTTCACTTGTTCAGCAACCAGATCAAAAGCCATTGACTCAGCAAGGGTTGACCGTATAATTCACGCGATTACACCGCATTGCGGTATCAACGCGCCCTTAGCTCAGTTGGATAGAGCAACGACCTTCTAAGTCGTGGGCCGCAGGt                                                                                                              >  2:345355/1‑150 (MQ=255)
                                         ggTCGTTCACTTGTTCAGCAACCAGATCAAAAGCCATTGACTCAGCAAGGGTTGACCGTATAATTCACGCGATTACACCGCATTGCGGTATCAACGCGCCCTTAGCTCAGTTGGATAGAGCAACGACCTTCTAAGTCGTGGGCCGCAGGt                                                                                                              >  2:4920660/1‑150 (MQ=255)
                                         ggTCGTTCACTTGTTCAGCAACCAGATCAAAAGCCATTGACTCAGCAAGGGTTGACCGTATAATTCACGCGATTACACCGCATTGCGGTATCAACGCGCCCTTAGCTCAGTTGGATAGAGCAACGACCTTCTAAGTCGTGGGCCGCAGGt                                                                                                              >  2:781812/1‑150 (MQ=255)
                                          gTCGTTCACTTGTTCAGCAACCAGATCAAAAGCCATTGACTCAGCAAGGGTTGACCGTATAATTCACGCGATTACACCGCATTGCGGTATCAACGCGCCCTTAGCTCAGTTGGATAGAGCAACGACCTTCTAAGTCGTGGGCCGCAGGtt                                                                                                             <  2:3897834/150‑1 (MQ=255)
                                             gTTCACTTGTTCAGCAACCAGATCAAAAGCCATTGACTCAGCAAGGGTTGACCGTATAATTCACGCGATTACACCGCATTGCGGTATCAACGCGCCCTTAGCTCAGTTGGATAGAGCAACGACCTTCTAAGTCGTGGGCCGCAGGTTCGa                                                                                                          >  1:1086200/1‑150 (MQ=255)
                                             gTTCACTTGTTCAGCAACCAGATCAAAAGCCATTGACTCAGCAAGGGTTGACCGTATAATTCACGCGATTACACCGCATTGCGGTATCAACGCGCCCTTAGCTCAGTTGGATAGAGCAACGACCTTCTAAGTCGTGGGCCGCAGGTTCGa                                                                                                          >  1:1086280/1‑150 (MQ=255)
                                                 aCTTGTTCAGCAACCAGATCAAAAGCCATTGACTCAGCAAGGGTTGACCGTATAATTCACGCGATTACACCGCATTGCGGTATCAACGCGCCCTTAGCTCCGTTGGATAGAGCAACGACCTTCTAAGTCGTGGGCCGCAGGTTCGAATcc                                                                                                      <  1:63529/150‑1 (MQ=255)
                                                 aCTTGTTCAGCAACCAGATCAAAAGCCATTGACTCAGCAAGGGTTGACCGTATAATTCACGCGATTACACCGCATTGCGGTATCAACGCGCCCTTAGCTCAGTTGGATAGAGCAACGACCTTCTAAGTCGTGGGCCGCAGGTTCGAATcc                                                                                                      <  2:1604563/150‑1 (MQ=255)
                                                  cTTGTTCAGCAACCAGATCAAAAGCCATTGACTCAGCAAGGGTTGACCGTATAATTCACGCGATTACACCGCATTGCGGTATCAACGCGCCCTTAGCTCCGTTGGATAGAGCAACGACCTTCTAAGTCGTGGGCCGCAGGTTCGAATCCt                                                                                                     <  1:2043467/150‑1 (MQ=255)
                                                   ttGTTCAGCAACCAGATCAAAAGCCATTGACTCAGCAAGGGTTGACCGTATAATTCACGCGATTACACCGCATTGCGGTATCAACGCGCCCTTAGCTCAGTTGGATAGAGCAACGACCTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTg                                                                                                    >  1:474117/1‑150 (MQ=255)
                                                     gTTCAGCAACCAGATCAAAAGCCATTGACTCAGCAAGGGTTGACCGTATAATTCACGCGATTACACCGCATTGCGGTATCAACGCGCCCTTAGCTCCGTTGGATAGAGCAACGACCTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCa                                                                                                  <  1:3787631/150‑1 (MQ=255)
                                                     gTTCAGCAACCAGATCAAAAGCCATTGACTCAGCAAGGGTTGACCGTATAATTCACGCGATTACACCGCATTGCGGTATCAACGCGCCCTTAGCTCAGTTGGATAGAGCAACGACCTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCa                                                                                                  <  2:793155/150‑1 (MQ=255)
                                                       tCAGCAACCAGATCAAAAGCCATTGACTCAGCAAGGGTTGACCGTATAATTCACGCGATTACACCGCATTGCGGTATCAACGCGCCCTTAGCTCCGTTGGATAGAGCAACGACCTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAgg                                                                                                <  1:479667/150‑1 (MQ=255)
                                                        cAGCAACCAGATCAAAAGCCATTGACTCAGCAAGGGTTGACCGTATAATTCACGCGATTACACCGCATTGCGGTATCAACGCGCCCTTAGCTCAGTTGGATAGAGCAACGACCTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAggg                                                                                               >  1:5193374/1‑150 (MQ=255)
                                                          gCAACCAGATCAAAAGCCATTGACTCAGCAAGGGTTGACCGTATAATTCACGCGATTACACCGCATTGCGGTATCAACGCGCCCTTAGCTCAGTTGGATAGAGCAACGACCTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGgcg                                                                                             <  2:1305370/150‑1 (MQ=255)
                                                           cAACCAGATCAAAAGCCATTGACTCAGCAAGGGTTGACCGTATAATTCACGCGATTACACCGCATTGCGGTATCAACGCGCCCTTAGCTCAGTTGGATAGAGCAACGACCTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGgcgc                                                                                            >  2:52402/1‑150 (MQ=255)
                                                           cAACCAGATCAAAAGCCATTGACTCAGCAAGGGTTGACCGTATAATTCACGCGATTACACCGCATTGCGGTATCAACGCGCCCTTAGCTCAGTTGGATAGAGCAACGACCTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGgcgc                                                                                            >  2:4052198/1‑150 (MQ=255)
                                                               cAGATCAAAAGCCATTGACTCAGCAAGGGTTGACCGTATAATTCACGCGATTACACCGCATTGCGGTATCAACGCGCCCTTAGCTCAGTTGGATAGAGCAACGACCTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCa                                                                                        <  2:1492879/150‑1 (MQ=255)
                                                                aGATCAAAAGCCATTGACTCAGCAAGGGTTGACCGTATAATTCACGCGATTACACCGCATTGCGGTATCAACGCGCCCTTAGCTCAGTTGGATAGAGCAACGACCTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCAt                                                                                       >  1:4344050/1‑150 (MQ=255)
                                                                   tCAAAAGCCATTGACTCAGCAAGGGTTGACCGTATAATTCACGCGATTACACCGCATTGCGGTATCAACGCGCCCTTAGCTCAGTTGGATAGAGCAACGACCTTCTACGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTAc                                                                                    <  2:5039973/150‑1 (MQ=255)
                                                                     aaaaGCCATTGACTCAGCAAGGGTTGACCGTATAATTCACGCGATTACACCGCATTGCGGTATCAACGCGCCCTTAGCTCAGTTGGATAGAGCAACGACCTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTACaa                                                                                  <  2:2693876/150‑1 (MQ=255)
                                                                     aaaaGCCATTGACTCAGCAAGGGTTGACCGTATAATTCACGCGATTACACCGCATTGCGGTATCAACGCGCCCTTAGCTCAGTTGGATAGAGCAACGACCTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTACaa                                                                                  <  1:2595069/150‑1 (MQ=255)
                                                                     aaaaGCCATTGACTCAGCAAGGGTTGACCGTATAATTCACGCGATTACACCGCATTGCGGTATCAACGCGCCCTTAGCTCAGTTGGATAGAGCAACGACCTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTACaa                                                                                  >  1:353632/1‑150 (MQ=255)
                                                                     aaaaGCCATTGACTCAGCAAGGGTTGACCGTATAATTCACGCGATTACACCGCATTGCGGTATCAACGCGCCCTTAGCTCAGTTGGATAGAGCAACGACCTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTACaa                                                                                  >  1:3523531/1‑150 (MQ=255)
                                                                      aaaGCCATTGACTCAGCAAGGGTTGACCGTATAATTCACGCGATTACACCGCATTGCGGTATCAACGCGCCCTTAGCTCAGTTGGATAGAGCAACGACCTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTACAAt                                                                                 <  1:1127287/150‑1 (MQ=255)
                                                                       aaGCCATTGACTCAGCAAGGGTTGACCGTATAATTCACGCGATTACACCGCATTGCGGTATCAACGCGCCCTTAGCTCAGTTGGATAGAGCAACGACCTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTACAAtt                                                                                <  2:4191740/150‑1 (MQ=255)
                                                                           cATTGACTCAGCAAGGGTTGACCGTATAATTCACGCGATTACACCGCATTGCGGTATCAACGCGCCCTTAGCTCAGTTGGATAGAGCAACGACCTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTACAATTCAAt                                                                            >  2:4586499/1‑150 (MQ=255)
                                                                           cATTGACTCAGCAAGGGTTGACCGTATAATTCACGCGATTACACCGCATTGCGGTATCAACGCGCCCTTAGCTCAGTTGGATAGAGCAACGACCTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTACAATTCAAt                                                                            <  1:2690716/150‑1 (MQ=255)
                                                                            aTTGACTCAGCAAGGGTTGACCGTATAATTCACGCGATTACACCGCATTGCGGTATCAACGCGCCCTTAGCTCAGTTGGATAGAGCAACGACCTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTACAATTCAATc                                                                           <  2:1598187/150‑1 (MQ=255)
                                                                            aTTGACTCAGCAAGGGTTGACCGTATAATTCACGCGATTACACCGCATTGCGGTATCAACGCGCCCTTAGCTCAGTTGGATAGAGCAACGACCTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTACAATTCAATc                                                                           <  2:4235770/150‑1 (MQ=255)
                                                                                 cTCAGCAAGGGTTGACCGTATAATTCACGCGATTACACCGCATTGCGGTATCAACGCGCCCTTAGCTCAGTTGGATAGAGCAACGACCTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTACAATTCAATCAGTTa                                                                      >  2:474831/1‑150 (MQ=255)
                                                                                 cTCAGCAAGAGTTGACCGTATAATTCACGCGATTACACCGCATTGCGGTATCAACGCGCCCTTAGCTCAGTTGGATAGAGCAACGACCTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTACAATTCAATCAGTTa                                                                      >  1:4050940/1‑150 (MQ=255)
                                                                                   cAGCAAGGGTTGACCGTATAATTCACGCGATTACACCGCATTGCGGTATCAACGCGCCCTTAGCTCAGTTGGATAGAGCAACGACCTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTACAATTCAATCAGTTACg                                                                    >  1:127470/1‑150 (MQ=255)
                                                                                      cAAGGGTTGACCGTATAATTCACGCGATTACACCGCATTGCGGTATCAACGCGCCCTTAGCTCAGTTGGATAGAGCAACGACCTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTACAATTCAATCAGTTACGCct                                                                 <  1:1123322/150‑1 (MQ=255)
                                                                                          ggTTGACCGTATAATTCACGCGATTACACCGCATTGCGGTATCAACGCGCCCTTAGCTCCGTTGGATAGAGCAACGACCTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTACAATTCAATCAGTTACGCcttctt                                                             <  1:189342/150‑1 (MQ=255)
                                                                                          ggTTGACCGTATAATTCACGCGATTACACCGCATTGCGGTATCAACGCGCCCTTAGCTCAGTTGGATAGAGCAACGACCTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTACAATTCAATCAGTTACGCcttctt                                                             <  1:4760966/150‑1 (MQ=255)
                                                                                            ttGACCGTATAATTCACGCGATTACACCGCATTGCGGTATCAACGCGCCCTTAGCTCCGTTGGATAGAGCAACGACCTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTACAATTCAATCAGTTACGCCTTCTTta                                                           <  1:768413/150‑1 (MQ=255)
                                                                                            ttGACCGTATAATTCACGCGATTACACCGCATTGCGGTATCAACGCGCCCTTAGCTCCGTTGGATAGAGCAACGACCTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTACAATTCAATCAGTTACGCCTTCTTta                                                           <  1:3364941/150‑1 (MQ=255)
                                                                                             tGACCGTATAATTCACGCGATTACACCGCATTGCGGTATCAACGCGCCCTTAGCTCAGTTGGATAGAGCAACGACCTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTACAATTCAATCAGTTACGCCTTCTTtat                                                          <  1:1225760/150‑1 (MQ=255)
                                                                                                ccGTATAATTCACGCGATTACACCGCATTGCGGTATCAACGCGCCCTTAGCTCAGTTGGATAGAGCAACGACCTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTACAATTCAATCAGTTACGCCTTCTTTATAtc                                                       <  2:1065916/150‑1 (MQ=255)
                                                                                                   tataATTCACGCGATTACACCGCATTGCGGTATCAACGCGCCCTTAGCTCAGTTGGATAGAGCAACGACCTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTACAATTCAATCAGTTACGCCTTCTTTATAtcctc                                                    >  1:711237/1‑150 (MQ=255)
                                                                                                    ataATTCACGCGATTACACCGCATTGCGGTATCAACGCGCCCTTAGCTCAGTTGGATAGAGCAACGACCTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTACAATTCAATCAGTTACGCCTTCTTTATAtcctcc                                                   <  2:2858964/150‑1 (MQ=255)
                                                                                                    ataATTCACGCGATTACACCGCATTGCGGTATCAACGCGCCCTGAGCTCAGTTGGATAGAGCAACGACCTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTACAATTCAATCAGTTACGCCTTCTTTATAtcctcc                                                   <  1:4438385/150‑1 (MQ=255)
                                                                                                         tCACGCGATTACACCGCATTGCGGTATCAACGCGCCCTTAGCTCAGTTGGATAGAGCAACGACCTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTACAATTCAATCAGTTACGCCTTCTTTATATCCTCCATAAt                                              >  1:413954/1‑150 (MQ=255)
                                                                                                         tCACGCGATTACACCGCATTGCGGTATCAACGCGCCCTTAGCTCAGTTGGATAGAGCAACGACCTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTACAATTCAATCAGTTACGCCTTCTTTATATCCTCCATAAt                                              >  1:893423/1‑150 (MQ=255)
                                                                                                           aCGCGATTACACCGCATTGCGGTATCAACGCGCCCTTAGCTCAGTTGGATAGAGCAACGACCTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTACAATTCAATCAGTTACGCCTTCTTTATATCCTCCATAAttt                                            <  1:2634031/150‑1 (MQ=255)
                                                                                                            cgcgATTACACCGCATTGCGGTATCAACGCGCCCTTAGCTCAGTTGGATAGAGCAACGACCTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTACAATTCAATCAGTTACGCCTTCTTTATATCCTCCATAATTTc                                           <  2:5049276/150‑1 (MQ=255)
                                                                                                              cgATTACACCGCATTGCGGTATCAACGCGCCCTTAGCTCAGTTGGATAGAGCAACGACCTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTACAATTCAATCAGTTACGCCTTCTTTATATCCTCCATAATTTCag                                         >  1:3761914/1‑150 (MQ=255)
                                                                                                                  tACACCGCATTGCGGTATCAACGCGCCCTTAGCTCAGTTGGATAGAGCAACGACCTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTACAATTCAATCAGTTACGCCTTCTTTATATCCTCCATAATTTCAGAGTg                                     >  1:3907467/1‑150 (MQ=255)
                                                                                                                  tACACCGCATTGCGGTATCAACGCGCCCTTAGCTCAGTTGGATAGAGCAACGACCTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTACAATTCAATCAGTTACGCCTTCTTTATATCCTCCATAATTTCAGAGTg                                     <  2:1758450/150‑1 (MQ=255)
                                                                                                                  tACACCGCATTGCGGTATCAACGCGCCCTTAGCTCAGTTGGATAGAGCAACGACCTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTACAATTCAATCAGTTACGCCTTCTTTATATCCTCCATAATTTCAGAGTg                                     >  1:3906983/1‑150 (MQ=255)
                                                                                                                   acacCGCATTGCGGTATCAACGCGCCCTTAGCTCAGTTGGATAGAGCAACGACCTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTACAATTCAATCAGTTACGCCTTCTTTATATCCTCCATAATTTCAGAGTgg                                    <  2:703434/150‑1 (MQ=255)
                                                                                                                   acacCGCATTGCGGTATCAACGCGCCCTTAGCTCAGTTGGATAGAGCAACGACCTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTACAATTCAATCAGTTACGCCTTCTTTATATCCTCCATAATTTCAGAGTgg                                    >  1:1623040/1‑150 (MQ=255)
                                                                                                                    cacCGCATTGCGGTATCAACGCGCCCTTAGCTCAGTTGGATAGAGCAACGACCTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTACAATTCAATCAGTTACGCCTTCTTTATATCCTCCATAATTTCAGAGTggg                                   <  1:425484/150‑1 (MQ=255)
                                                                                                                    cacCGCATTGCGGTATCAACGCGCCCTTAGCTCAGTTGGATAGAGCAACGACCTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTACAATTCAATCAGTTACGCCTTCTTTATATCCTCCATAATTTCAGAGTggg                                   >  1:4922467/1‑150 (MQ=255)
                                                                                                                     acCGCATTGCGGTATCAACGCGCCCTTAGCTCAGTTGGATAGAGCAACGACCTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTACAATTCAATCAGTTACGCCTTCTTTATATCCTCCATAATTTCAGAGTGGGa                                  <  2:479651/150‑1 (MQ=255)
                                                                                                                       cGCATTGCGGTATCAACGCGCCCTTAGCTCAGTTGGATAGAGCAACGACCTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTACAATTCAATCAGTTACGCCTTCTTTATATCCTCCATAATTTCAGAGTGGGACa                                >  2:1492456/1‑150 (MQ=255)
                                                                                                                         cATTGCGGTATCAACGCGCCCTTAGCTCAGTTGGATAGAGCAACGACCTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTACAATTCAATCAGTTACGCCTTCTTTATATCCTCCATAATTTCAGAGTGGGACata                              <  1:4417883/150‑1 (MQ=255)
                                                                                                                          aTTGCGGTATCAACGCGCCCTTAGCTCAGTTGGATAGAGCAACGACCTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTACAATTCAATCAGTTACGCCTTCTTTATATCCTCCATAATTTCAGAGTGGGACatat                             <  2:1522339/150‑1 (MQ=255)
                                                                                                                           ttGCGGTATCAACGCGCCCTTAGCTCAGTTGGATAGAGCAACGACCTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCCGGGCGCGCCATTACAATTCAATCAGTTACGCCTTCTGTATATCCTCCATAATTTCCGAGTGGGACATAtt                            >  2:665522/1‑150 (MQ=255)
                                                                                                                           ttGCGGTATCAACGCGCCCTTAGCTCAGTTGGATAGAGCAACGACCTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTACAATTCAATCAGTTACGCCTTCTTTATATCCTCCATAATTTCAGAGTGGGACATAtt                            <  1:3191207/150‑1 (MQ=255)
                                                                                                                                 tATCAACGCGCCCTTAGCTCAGTTGGATAGAGCAACGACCTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTACAATTCAATCAGTTACGCCTTCTTTATATCCTCCATAATTTCAGAGTGGGACATATTTGGGAc                      >  1:2743756/1‑150 (MQ=255)
                                                                                                                                    cAACGCGCCCTTAGCTCAGTTGGATAGAGCAACGACCTTCTAAGTCGTGGGCCGCAGGTTCGACTCCTGCAGGGCGCGCCATTACAATTCAATCAGTTACGCCTTCTTTATATCCTCCATAATTTCAGAGTGGGACATATTTGGGACAtt                   >  2:4634199/1‑150 (MQ=255)
                                                                                                                                     aaCGCGCCCTTAGCTCAGTTGGATAGAGCAACGACCTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTACAATTCAATCAGTTACGCCTTCTTTATATCCTCCATAATTTCAGAGTGGGACATATTTGGGACATTa                  <  2:2738649/150‑1 (MQ=255)
                                                                                                                                         cgcCCTTAGCTCAGTTGGATAGAGCAACGACCTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTACAATTCAATCAGTTACGCCTTCTTTATATCCTCCATAATTTCAGAGTGGGACATATTTGGGACATTATCAc              >  1:3282830/1‑150 (MQ=255)
                                                                                                                                           cccTTAGCTCAGTTGGATAGAGCAACGACCTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTACAATTCAATCAGTTACGCCTTCTTTATATCCTCCATAATTTCAGAGTGGGACATATTTGGGACATTATCACCa            <  1:4519209/150‑1 (MQ=255)
                                                                                                                                            ccTTAGCTCAGTTGGATAGAGCAACGACCTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTACAATTCAATCAGTTACGCCTTCTTTATATCCTCCATAATTTCAGAGTGGGACATATTTGGGACATTATCACCaa           <  2:230300/150‑1 (MQ=255)
                                                                                                                                             cTTAGCTCAGTTGGATAGAGCAACGACCTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTACAATTCAATCAGTTACGCCTTCTTTATATCCTCCATAATTTCAGAGTGGGACATATTTGGGACATTATCACCaaa          >  1:2801071/1‑150 (MQ=255)
                                                                                                                                               tAGCTCAGTTGGATAGAGCAACGACCTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTACAATTCAATCAGTTACGCCTTCTTTATATCCTCCATAATTTCAGAGTGGGACATATTTGGGACATTATCACCaaaaa        >  1:3019688/1‑150 (MQ=255)
                                                                                                                                                aGCTCAGTTGGATAGAGCAACGACCTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTACAATTCAATCAGTTACGCCTTCTTTATATCCTCCATAATTTCAGAGTGGGACATATTTGGGACATTATCACCAAAAAt       >  2:3618009/1‑150 (MQ=255)
                                                                                                                                                aGCTCAGTTGGATAGAGCAACGACCTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTACAATTCAATCAGTTACGCCTTCTTTATATCCTCCATAATTTCAGAGTGGGACATATTTGGGACATTATCACCAAAAAt       <  2:4344050/150‑1 (MQ=255)
                                                                                                                                                  cTCAGTTGGATAGAGCAACGACCTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTACAATTCAATCAGTTACGCCTTCTTTATATCCTCCATAATTTCAGAGTGGGACATATTTGGGACATTATCACCAAAAATgt     <  2:2899486/150‑1 (MQ=255)
                                                                                                                                                  cTCAGTTGGATAGAGCAACGACCTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTACAATTCAATCAGTTACGCCTTCTTTATATCCTCCATAATTTCAGAGTGGGACATATTTGGGACATTATCACCAAAAATgt     <  1:940783/150‑1 (MQ=255)
                                                                                                                                                     aGTTGGATAGAGCAACGACCTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCCGGGCGCGCCATTACAATTAAATCAGTTACGCCTTCTTTATATCATCCATAATTTCAGAGTGGGACATATTTGTGACATTATCACCAAAAATgtcgt  >  2:3605786/1‑150 (MQ=255)
                                                                                                                                                     aGTTGGATAGAGCAACGACCTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTACAATTCAATCAGTTACGCCTTCTTTATATCCTCCATAATTTCAGAGTGGGACATATTTGGGACATTATCACCAAAAATgtcgt  >  2:733833/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TTGTTTGCCGCTATAGCGAAATAAATCAGAAAATCAGACGCGGTCGTTCACTTGTTCAGCAACCAGATCAAAAGCCATTGACTCAGCAAGGGTTGACCGTATAATTCACGCGATTACACCGCATTGCGGTATCAACGCGCCCTTAGCTCAGTTGGATAGAGCAACGACCTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTACAATTCAATCAGTTACGCCTTCTTTATATCCTCCATAATTTCAGAGTGGGACATATTTGGGACATTATCACCAAAAATGTCGT  >  CP009273/560043‑560341

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑